Amino acid dipepetide frequency for Pseudomonas phage Psp6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.411AlaAla: 12.411 ± 1.698
0.919AlaCys: 0.919 ± 0.292
5.822AlaAsp: 5.822 ± 0.825
5.899AlaGlu: 5.899 ± 0.954
3.524AlaPhe: 3.524 ± 0.487
7.125AlaGly: 7.125 ± 0.864
2.375AlaHis: 2.375 ± 0.398
4.597AlaIle: 4.597 ± 0.478
6.052AlaLys: 6.052 ± 1.072
9.193AlaLeu: 9.193 ± 0.83
3.371AlaMet: 3.371 ± 0.537
4.52AlaAsn: 4.52 ± 0.681
4.29AlaPro: 4.29 ± 0.472
5.133AlaGln: 5.133 ± 0.808
3.294AlaArg: 3.294 ± 0.607
5.439AlaSer: 5.439 ± 0.62
5.363AlaThr: 5.363 ± 1.114
6.895AlaVal: 6.895 ± 0.652
2.681AlaTrp: 2.681 ± 0.484
2.222AlaTyr: 2.222 ± 0.436
0.0AlaXaa: 0.0 ± 0.0
Cys
1.839CysAla: 1.839 ± 0.468
0.153CysCys: 0.153 ± 0.163
0.843CysAsp: 0.843 ± 0.231
0.996CysGlu: 0.996 ± 0.267
0.383CysPhe: 0.383 ± 0.181
1.226CysGly: 1.226 ± 0.262
0.383CysHis: 0.383 ± 0.188
0.306CysIle: 0.306 ± 0.134
0.689CysLys: 0.689 ± 0.263
0.996CysLeu: 0.996 ± 0.252
0.306CysMet: 0.306 ± 0.196
0.996CysAsn: 0.996 ± 0.259
1.226CysPro: 1.226 ± 0.352
0.536CysGln: 0.536 ± 0.191
0.919CysArg: 0.919 ± 0.27
0.689CysSer: 0.689 ± 0.224
0.536CysThr: 0.536 ± 0.179
1.149CysVal: 1.149 ± 0.272
0.613CysTrp: 0.613 ± 0.258
0.306CysTyr: 0.306 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
6.052AspAla: 6.052 ± 0.706
0.919AspCys: 0.919 ± 0.245
3.524AspAsp: 3.524 ± 0.573
3.831AspGlu: 3.831 ± 0.663
2.068AspPhe: 2.068 ± 0.437
6.435AspGly: 6.435 ± 0.636
1.149AspHis: 1.149 ± 0.293
2.835AspIle: 2.835 ± 0.46
2.835AspLys: 2.835 ± 0.48
4.826AspLeu: 4.826 ± 0.515
1.456AspMet: 1.456 ± 0.297
1.532AspAsn: 1.532 ± 0.356
3.524AspPro: 3.524 ± 0.531
2.222AspGln: 2.222 ± 0.345
3.218AspArg: 3.218 ± 0.511
3.064AspSer: 3.064 ± 0.52
2.988AspThr: 2.988 ± 0.506
3.064AspVal: 3.064 ± 0.526
0.919AspTrp: 0.919 ± 0.237
1.762AspTyr: 1.762 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
5.976GluAla: 5.976 ± 0.726
1.073GluCys: 1.073 ± 0.238
3.447GluAsp: 3.447 ± 0.658
2.911GluGlu: 2.911 ± 0.574
2.452GluPhe: 2.452 ± 0.385
4.214GluGly: 4.214 ± 0.642
1.226GluHis: 1.226 ± 0.394
2.452GluIle: 2.452 ± 0.381
2.222GluLys: 2.222 ± 0.45
6.589GluLeu: 6.589 ± 0.879
2.068GluMet: 2.068 ± 0.448
1.379GluAsn: 1.379 ± 0.355
2.145GluPro: 2.145 ± 0.407
3.294GluGln: 3.294 ± 0.446
4.52GluArg: 4.52 ± 0.631
2.068GluSer: 2.068 ± 0.352
3.218GluThr: 3.218 ± 0.452
3.294GluVal: 3.294 ± 0.501
1.532GluTrp: 1.532 ± 0.421
1.609GluTyr: 1.609 ± 0.375
0.0GluXaa: 0.0 ± 0.0
Phe
2.988PheAla: 2.988 ± 0.434
0.613PheCys: 0.613 ± 0.216
2.145PheAsp: 2.145 ± 0.364
2.068PheGlu: 2.068 ± 0.387
0.919PhePhe: 0.919 ± 0.293
2.988PheGly: 2.988 ± 0.495
0.383PheHis: 0.383 ± 0.151
1.685PheIle: 1.685 ± 0.32
2.222PheLys: 2.222 ± 0.5
2.298PheLeu: 2.298 ± 0.431
0.536PheMet: 0.536 ± 0.212
2.298PheAsn: 2.298 ± 0.401
1.379PhePro: 1.379 ± 0.344
1.302PheGln: 1.302 ± 0.236
1.456PheArg: 1.456 ± 0.319
1.839PheSer: 1.839 ± 0.365
1.685PheThr: 1.685 ± 0.383
2.605PheVal: 2.605 ± 0.486
0.843PheTrp: 0.843 ± 0.289
1.379PheTyr: 1.379 ± 0.359
0.0PheXaa: 0.0 ± 0.0
Gly
7.125GlyAla: 7.125 ± 0.79
1.302GlyCys: 1.302 ± 0.379
4.826GlyAsp: 4.826 ± 0.622
4.903GlyGlu: 4.903 ± 0.58
3.754GlyPhe: 3.754 ± 0.553
5.976GlyGly: 5.976 ± 0.663
1.532GlyHis: 1.532 ± 0.274
3.601GlyIle: 3.601 ± 0.673
4.29GlyLys: 4.29 ± 0.695
6.818GlyLeu: 6.818 ± 0.541
2.681GlyMet: 2.681 ± 0.42
3.218GlyAsn: 3.218 ± 0.504
2.835GlyPro: 2.835 ± 0.46
2.758GlyGln: 2.758 ± 0.547
4.29GlyArg: 4.29 ± 0.505
4.214GlySer: 4.214 ± 0.671
5.056GlyThr: 5.056 ± 0.838
6.895GlyVal: 6.895 ± 0.579
2.452GlyTrp: 2.452 ± 0.439
3.064GlyTyr: 3.064 ± 0.472
0.0GlyXaa: 0.0 ± 0.0
His
2.452HisAla: 2.452 ± 0.465
0.46HisCys: 0.46 ± 0.254
1.379HisAsp: 1.379 ± 0.263
0.766HisGlu: 0.766 ± 0.225
0.766HisPhe: 0.766 ± 0.222
1.456HisGly: 1.456 ± 0.256
0.689HisHis: 0.689 ± 0.23
1.302HisIle: 1.302 ± 0.327
0.613HisLys: 0.613 ± 0.252
1.226HisLeu: 1.226 ± 0.339
0.689HisMet: 0.689 ± 0.241
0.306HisAsn: 0.306 ± 0.174
1.302HisPro: 1.302 ± 0.357
0.689HisGln: 0.689 ± 0.211
1.226HisArg: 1.226 ± 0.308
1.073HisSer: 1.073 ± 0.333
0.766HisThr: 0.766 ± 0.223
1.609HisVal: 1.609 ± 0.413
0.613HisTrp: 0.613 ± 0.207
1.149HisTyr: 1.149 ± 0.3
0.0HisXaa: 0.0 ± 0.0
Ile
5.363IleAla: 5.363 ± 0.692
0.766IleCys: 0.766 ± 0.208
4.06IleAsp: 4.06 ± 0.578
3.831IleGlu: 3.831 ± 0.5
1.073IlePhe: 1.073 ± 0.226
3.601IleGly: 3.601 ± 0.43
0.996IleHis: 0.996 ± 0.251
1.992IleIle: 1.992 ± 0.387
2.452IleLys: 2.452 ± 0.525
2.835IleLeu: 2.835 ± 0.444
0.843IleMet: 0.843 ± 0.277
2.222IleAsn: 2.222 ± 0.396
1.992IlePro: 1.992 ± 0.294
2.222IleGln: 2.222 ± 0.513
2.681IleArg: 2.681 ± 0.45
2.222IleSer: 2.222 ± 0.443
2.375IleThr: 2.375 ± 0.465
2.911IleVal: 2.911 ± 0.449
0.689IleTrp: 0.689 ± 0.25
1.609IleTyr: 1.609 ± 0.389
0.0IleXaa: 0.0 ± 0.0
Lys
5.822LysAla: 5.822 ± 0.578
0.919LysCys: 0.919 ± 0.283
2.298LysAsp: 2.298 ± 0.485
3.677LysGlu: 3.677 ± 0.578
1.456LysPhe: 1.456 ± 0.358
4.06LysGly: 4.06 ± 0.428
1.226LysHis: 1.226 ± 0.319
2.375LysIle: 2.375 ± 0.424
2.528LysLys: 2.528 ± 0.481
4.597LysLeu: 4.597 ± 0.481
1.302LysMet: 1.302 ± 0.305
1.302LysAsn: 1.302 ± 0.277
2.988LysPro: 2.988 ± 0.474
1.915LysGln: 1.915 ± 0.349
3.601LysArg: 3.601 ± 0.63
2.528LysSer: 2.528 ± 0.572
2.375LysThr: 2.375 ± 0.441
3.677LysVal: 3.677 ± 0.416
0.766LysTrp: 0.766 ± 0.246
1.302LysTyr: 1.302 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
9.27LeuAla: 9.27 ± 0.951
1.456LeuCys: 1.456 ± 0.327
5.516LeuAsp: 5.516 ± 0.532
5.286LeuGlu: 5.286 ± 0.807
2.528LeuPhe: 2.528 ± 0.427
6.052LeuGly: 6.052 ± 0.607
1.609LeuHis: 1.609 ± 0.474
2.758LeuIle: 2.758 ± 0.407
4.673LeuLys: 4.673 ± 0.806
6.052LeuLeu: 6.052 ± 0.674
1.839LeuMet: 1.839 ± 0.45
3.064LeuAsn: 3.064 ± 0.582
6.665LeuPro: 6.665 ± 1.088
3.218LeuGln: 3.218 ± 0.593
6.972LeuArg: 6.972 ± 0.88
5.056LeuSer: 5.056 ± 0.522
4.137LeuThr: 4.137 ± 0.637
5.516LeuVal: 5.516 ± 0.639
1.456LeuTrp: 1.456 ± 0.341
2.068LeuTyr: 2.068 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
2.835MetAla: 2.835 ± 0.434
0.46MetCys: 0.46 ± 0.209
1.226MetAsp: 1.226 ± 0.256
1.456MetGlu: 1.456 ± 0.291
0.919MetPhe: 0.919 ± 0.292
1.379MetGly: 1.379 ± 0.334
0.613MetHis: 0.613 ± 0.24
1.609MetIle: 1.609 ± 0.292
1.226MetLys: 1.226 ± 0.333
3.064MetLeu: 3.064 ± 0.505
0.766MetMet: 0.766 ± 0.248
1.149MetAsn: 1.149 ± 0.308
1.992MetPro: 1.992 ± 0.474
1.073MetGln: 1.073 ± 0.259
1.992MetArg: 1.992 ± 0.407
2.298MetSer: 2.298 ± 0.415
1.302MetThr: 1.302 ± 0.345
1.685MetVal: 1.685 ± 0.409
0.23MetTrp: 0.23 ± 0.117
0.919MetTyr: 0.919 ± 0.282
0.0MetXaa: 0.0 ± 0.0
Asn
3.294AsnAla: 3.294 ± 0.539
0.613AsnCys: 0.613 ± 0.343
1.685AsnAsp: 1.685 ± 0.455
1.609AsnGlu: 1.609 ± 0.37
1.609AsnPhe: 1.609 ± 0.341
3.371AsnGly: 3.371 ± 0.483
0.536AsnHis: 0.536 ± 0.188
1.915AsnIle: 1.915 ± 0.469
1.532AsnLys: 1.532 ± 0.261
3.447AsnLeu: 3.447 ± 0.498
1.302AsnMet: 1.302 ± 0.294
1.379AsnAsn: 1.379 ± 0.227
2.681AsnPro: 2.681 ± 0.641
1.302AsnGln: 1.302 ± 0.352
1.609AsnArg: 1.609 ± 0.331
2.145AsnSer: 2.145 ± 0.427
1.609AsnThr: 1.609 ± 0.341
3.141AsnVal: 3.141 ± 0.593
0.536AsnTrp: 0.536 ± 0.185
1.226AsnTyr: 1.226 ± 0.278
0.0AsnXaa: 0.0 ± 0.0
Pro
4.06ProAla: 4.06 ± 0.598
0.613ProCys: 0.613 ± 0.235
3.524ProAsp: 3.524 ± 0.51
3.371ProGlu: 3.371 ± 0.473
0.996ProPhe: 0.996 ± 0.222
5.822ProGly: 5.822 ± 0.79
1.762ProHis: 1.762 ± 0.349
2.911ProIle: 2.911 ± 0.412
2.835ProLys: 2.835 ± 0.379
4.214ProLeu: 4.214 ± 0.545
1.915ProMet: 1.915 ± 0.34
1.226ProAsn: 1.226 ± 0.225
3.754ProPro: 3.754 ± 0.897
2.452ProGln: 2.452 ± 0.65
2.375ProArg: 2.375 ± 0.421
2.222ProSer: 2.222 ± 0.47
4.214ProThr: 4.214 ± 0.631
3.524ProVal: 3.524 ± 0.49
1.073ProTrp: 1.073 ± 0.285
1.532ProTyr: 1.532 ± 0.374
0.0ProXaa: 0.0 ± 0.0
Gln
4.137GlnAla: 4.137 ± 0.723
0.306GlnCys: 0.306 ± 0.203
0.996GlnAsp: 0.996 ± 0.222
1.762GlnGlu: 1.762 ± 0.422
1.532GlnPhe: 1.532 ± 0.31
3.677GlnGly: 3.677 ± 0.617
0.919GlnHis: 0.919 ± 0.338
2.145GlnIle: 2.145 ± 0.53
1.456GlnLys: 1.456 ± 0.473
5.133GlnLeu: 5.133 ± 0.678
1.532GlnMet: 1.532 ± 0.337
1.532GlnAsn: 1.532 ± 0.436
2.528GlnPro: 2.528 ± 0.588
2.145GlnGln: 2.145 ± 0.654
2.835GlnArg: 2.835 ± 0.351
1.456GlnSer: 1.456 ± 0.329
2.452GlnThr: 2.452 ± 0.534
2.911GlnVal: 2.911 ± 0.519
0.306GlnTrp: 0.306 ± 0.138
0.996GlnTyr: 0.996 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
4.903ArgAla: 4.903 ± 0.718
0.996ArgCys: 0.996 ± 0.253
3.064ArgAsp: 3.064 ± 0.506
3.447ArgGlu: 3.447 ± 0.425
1.839ArgPhe: 1.839 ± 0.422
4.597ArgGly: 4.597 ± 0.653
1.149ArgHis: 1.149 ± 0.319
3.218ArgIle: 3.218 ± 0.508
3.831ArgLys: 3.831 ± 0.647
6.129ArgLeu: 6.129 ± 0.689
1.915ArgMet: 1.915 ± 0.415
1.685ArgAsn: 1.685 ± 0.411
2.375ArgPro: 2.375 ± 0.468
1.685ArgGln: 1.685 ± 0.23
2.605ArgArg: 2.605 ± 0.576
2.298ArgSer: 2.298 ± 0.423
3.294ArgThr: 3.294 ± 0.527
4.137ArgVal: 4.137 ± 0.56
1.915ArgTrp: 1.915 ± 0.384
1.992ArgTyr: 1.992 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
6.742SerAla: 6.742 ± 0.861
0.766SerCys: 0.766 ± 0.256
3.218SerAsp: 3.218 ± 0.469
2.528SerGlu: 2.528 ± 0.49
1.609SerPhe: 1.609 ± 0.275
4.52SerGly: 4.52 ± 0.606
0.919SerHis: 0.919 ± 0.287
2.375SerIle: 2.375 ± 0.381
2.911SerLys: 2.911 ± 0.338
3.601SerLeu: 3.601 ± 0.625
1.456SerMet: 1.456 ± 0.273
2.068SerAsn: 2.068 ± 0.298
1.915SerPro: 1.915 ± 0.316
2.375SerGln: 2.375 ± 0.38
2.145SerArg: 2.145 ± 0.402
2.758SerSer: 2.758 ± 0.781
3.371SerThr: 3.371 ± 0.494
3.524SerVal: 3.524 ± 0.429
1.456SerTrp: 1.456 ± 0.348
1.226SerTyr: 1.226 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
5.593ThrAla: 5.593 ± 1.071
0.766ThrCys: 0.766 ± 0.279
2.988ThrAsp: 2.988 ± 0.502
2.988ThrGlu: 2.988 ± 0.471
2.835ThrPhe: 2.835 ± 0.444
5.286ThrGly: 5.286 ± 0.687
1.073ThrHis: 1.073 ± 0.288
3.447ThrIle: 3.447 ± 0.572
2.145ThrLys: 2.145 ± 0.435
3.907ThrLeu: 3.907 ± 0.505
1.149ThrMet: 1.149 ± 0.265
2.452ThrAsn: 2.452 ± 0.475
3.831ThrPro: 3.831 ± 0.529
2.222ThrGln: 2.222 ± 0.384
2.528ThrArg: 2.528 ± 0.451
2.681ThrSer: 2.681 ± 0.53
4.137ThrThr: 4.137 ± 0.622
4.673ThrVal: 4.673 ± 0.78
1.226ThrTrp: 1.226 ± 0.333
1.839ThrTyr: 1.839 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
5.746ValAla: 5.746 ± 0.491
0.766ValCys: 0.766 ± 0.269
3.601ValAsp: 3.601 ± 0.501
4.137ValGlu: 4.137 ± 0.548
1.685ValPhe: 1.685 ± 0.401
5.822ValGly: 5.822 ± 0.803
1.073ValHis: 1.073 ± 0.275
3.064ValIle: 3.064 ± 0.457
3.677ValLys: 3.677 ± 0.526
5.899ValLeu: 5.899 ± 0.715
1.456ValMet: 1.456 ± 0.32
2.528ValAsn: 2.528 ± 0.355
4.443ValPro: 4.443 ± 0.627
2.528ValGln: 2.528 ± 0.501
4.98ValArg: 4.98 ± 0.729
4.98ValSer: 4.98 ± 0.603
5.822ValThr: 5.822 ± 0.954
5.593ValVal: 5.593 ± 0.738
1.226ValTrp: 1.226 ± 0.323
2.068ValTyr: 2.068 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
1.839TrpAla: 1.839 ± 0.348
0.613TrpCys: 0.613 ± 0.185
1.685TrpAsp: 1.685 ± 0.359
0.996TrpGlu: 0.996 ± 0.215
0.996TrpPhe: 0.996 ± 0.332
1.456TrpGly: 1.456 ± 0.341
0.383TrpHis: 0.383 ± 0.132
1.302TrpIle: 1.302 ± 0.326
0.766TrpLys: 0.766 ± 0.22
2.222TrpLeu: 2.222 ± 0.354
0.46TrpMet: 0.46 ± 0.156
0.536TrpAsn: 0.536 ± 0.162
0.919TrpPro: 0.919 ± 0.275
0.689TrpGln: 0.689 ± 0.276
1.839TrpArg: 1.839 ± 0.475
1.302TrpSer: 1.302 ± 0.304
0.996TrpThr: 0.996 ± 0.262
1.379TrpVal: 1.379 ± 0.293
0.689TrpTrp: 0.689 ± 0.168
0.843TrpTyr: 0.843 ± 0.273
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.758TyrAla: 2.758 ± 0.44
0.613TyrCys: 0.613 ± 0.21
2.375TyrAsp: 2.375 ± 0.472
1.226TyrGlu: 1.226 ± 0.237
0.689TyrPhe: 0.689 ± 0.191
2.528TyrGly: 2.528 ± 0.484
0.536TyrHis: 0.536 ± 0.198
0.843TyrIle: 0.843 ± 0.244
1.762TyrLys: 1.762 ± 0.331
2.145TyrLeu: 2.145 ± 0.328
1.073TyrMet: 1.073 ± 0.296
1.149TyrAsn: 1.149 ± 0.284
1.609TyrPro: 1.609 ± 0.346
0.996TyrGln: 0.996 ± 0.302
1.992TyrArg: 1.992 ± 0.388
1.149TyrSer: 1.149 ± 0.263
2.068TyrThr: 2.068 ± 0.532
2.911TyrVal: 2.911 ± 0.364
0.689TyrTrp: 0.689 ± 0.215
1.149TyrTyr: 1.149 ± 0.293
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13054 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski