Aquimarina intermedia

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Aquimarina

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S5C533|A0A5S5C533_9FLAO Enolase OS=Aquimarina intermedia OX=350814 GN=eno PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.25FFKK5 pKa = 9.8HH6 pKa = 5.04TFRR9 pKa = 11.84LFLLLLIIQGCNDD22 pKa = 4.51DD23 pKa = 6.06DD24 pKa = 4.97NFDD27 pKa = 3.94YY28 pKa = 11.05LNKK31 pKa = 9.84VDD33 pKa = 4.53APANIAAAIQLTQDD47 pKa = 2.93NTGLVTITPKK57 pKa = 10.94GDD59 pKa = 3.18GVVSFDD65 pKa = 4.15IYY67 pKa = 11.06FGDD70 pKa = 3.69EE71 pKa = 3.73TEE73 pKa = 4.53APVNIQLGEE82 pKa = 4.45VITHH86 pKa = 7.48AYY88 pKa = 8.6TEE90 pKa = 4.19GTYY93 pKa = 10.54DD94 pKa = 4.23LRR96 pKa = 11.84IIGIGITGLKK106 pKa = 9.02TEE108 pKa = 5.88AIQQVVISFNAPEE121 pKa = 4.03NLEE124 pKa = 4.07VVIANDD130 pKa = 3.37EE131 pKa = 4.25ATSKK135 pKa = 10.15QVNVTATADD144 pKa = 3.5YY145 pKa = 11.57AMSFDD150 pKa = 3.9VYY152 pKa = 10.69FGEE155 pKa = 4.75EE156 pKa = 4.26GNDD159 pKa = 3.42APVSATIGEE168 pKa = 4.51VASYY172 pKa = 9.08TYY174 pKa = 9.32QQPGTYY180 pKa = 8.71TIKK183 pKa = 10.69VVAKK187 pKa = 9.38GGAIATTEE195 pKa = 3.86YY196 pKa = 11.17SEE198 pKa = 4.27VFEE201 pKa = 4.34VTEE204 pKa = 3.79ILQPVASAPTPPARR218 pKa = 11.84AAQDD222 pKa = 3.43VMSVFSAAYY231 pKa = 8.14TDD233 pKa = 4.22EE234 pKa = 5.27PGTDD238 pKa = 3.88YY239 pKa = 11.16FPDD242 pKa = 3.48WGQGSQGSSWSLFDD256 pKa = 5.2LNGDD260 pKa = 4.61AMLQYY265 pKa = 10.94INLSYY270 pKa = 10.81QGIQFGAAVDD280 pKa = 3.87VSAMEE285 pKa = 4.47FFHH288 pKa = 6.43MDD290 pKa = 2.32VWTSGDD296 pKa = 3.18VTAIEE301 pKa = 4.44TSLISQTNGEE311 pKa = 4.54KK312 pKa = 10.47PFSNDD317 pKa = 2.58LVAGQWTAIDD327 pKa = 3.73IPISAFTDD335 pKa = 3.18QGLTVADD342 pKa = 3.28IHH344 pKa = 5.73QLKK347 pKa = 10.14FVGTPWAEE355 pKa = 3.58GTVFIDD361 pKa = 4.21NIYY364 pKa = 9.89FYY366 pKa = 10.35KK367 pKa = 10.91GSAQTVTAAPTPTVAAANVISMFSDD392 pKa = 3.9AYY394 pKa = 10.1TDD396 pKa = 3.51VTVDD400 pKa = 2.87TWRR403 pKa = 11.84TSWSDD408 pKa = 3.15AVLEE412 pKa = 4.28DD413 pKa = 3.51VTVEE417 pKa = 3.93GNATKK422 pKa = 10.24KK423 pKa = 10.51YY424 pKa = 9.79SALNFVGIEE433 pKa = 4.13TTSAPIDD440 pKa = 3.67ATAMTHH446 pKa = 5.52FHH448 pKa = 6.42TDD450 pKa = 2.33VWSDD454 pKa = 3.47DD455 pKa = 3.4FTEE458 pKa = 4.83FKK460 pKa = 10.53IKK462 pKa = 10.66LVDD465 pKa = 4.66FGADD469 pKa = 3.22GLFQGGDD476 pKa = 3.68DD477 pKa = 4.02VEE479 pKa = 4.73HH480 pKa = 7.47EE481 pKa = 4.28ITISNPAVGEE491 pKa = 4.21WVSLAIPLSDD501 pKa = 3.43FTGLTTRR508 pKa = 11.84ANIAQLIYY516 pKa = 10.6VGSPTGATTVYY527 pKa = 9.65IDD529 pKa = 3.64NVYY532 pKa = 10.36FHH534 pKa = 6.95NN535 pKa = 4.37

Molecular weight: 57.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S5BX28|A0A5S5BX28_9FLAO Ribosomal protein S18 acetylase RimI-like enzyme OS=Aquimarina intermedia OX=350814 GN=BD809_11124 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.66LSVSSEE47 pKa = 3.9LRR49 pKa = 11.84HH50 pKa = 5.43KK51 pKa = 10.29HH52 pKa = 5.04

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3132

0

3132

1033828

29

5960

330.1

37.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.566 ± 0.052

0.738 ± 0.016

5.686 ± 0.035

6.609 ± 0.048

5.04 ± 0.038

6.278 ± 0.059

1.863 ± 0.022

8.052 ± 0.045

7.452 ± 0.074

9.339 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.023

5.686 ± 0.04

3.374 ± 0.03

3.594 ± 0.028

3.692 ± 0.028

6.481 ± 0.033

6.124 ± 0.069

6.241 ± 0.038

0.98 ± 0.016

4.053 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski