Lactobacillus phage JNU_P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BDY2|A0A6M3BDY2_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P1 OX=2686379 PE=4 SV=1
MM1 pKa = 7.21ATMIKK6 pKa = 9.68FRR8 pKa = 11.84AWDD11 pKa = 3.62KK12 pKa = 11.36GNEE15 pKa = 3.69IYY17 pKa = 10.57LYY19 pKa = 10.12NVQDD23 pKa = 4.33AYY25 pKa = 10.66DD26 pKa = 3.89TLSGNVTYY34 pKa = 10.86DD35 pKa = 3.49DD36 pKa = 5.84GEE38 pKa = 4.34DD39 pKa = 3.06ACYY42 pKa = 10.88DD43 pKa = 3.85EE44 pKa = 6.24DD45 pKa = 5.36CAHH48 pKa = 5.67QQQ50 pKa = 3.17

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BDW0|A0A6M3BDW0_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P1 OX=2686379 PE=4 SV=1
MM1 pKa = 7.3FNLVEE6 pKa = 4.19RR7 pKa = 11.84RR8 pKa = 11.84LKK10 pKa = 10.24EE11 pKa = 3.8LPEE14 pKa = 4.24VIKK17 pKa = 10.98DD18 pKa = 3.52DD19 pKa = 3.78AYY21 pKa = 10.13KK22 pKa = 10.6VLRR25 pKa = 11.84STIEE29 pKa = 3.93SRR31 pKa = 11.84GLKK34 pKa = 9.29QNYY37 pKa = 4.9VAKK40 pKa = 10.6RR41 pKa = 11.84IGITPNYY48 pKa = 9.16LASILNGRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84LNANIAIRR66 pKa = 11.84ASQVLEE72 pKa = 3.85IPLDD76 pKa = 3.08IFLRR80 pKa = 11.84KK81 pKa = 9.26NN82 pKa = 3.0

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15769

36

1891

216.0

24.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.466 ± 0.539

0.457 ± 0.089

6.912 ± 0.388

5.745 ± 0.482

3.424 ± 0.229

6.494 ± 0.474

1.566 ± 0.136

6.151 ± 0.222

7.223 ± 0.46

8.162 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.263

6.227 ± 0.255

2.733 ± 0.276

4.61 ± 0.199

4.211 ± 0.342

7.242 ± 0.549

7.191 ± 0.633

5.72 ± 0.257

1.389 ± 0.108

3.646 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski