Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3580 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|A7HTA7|Y1521_PARL1 UPF0178 protein Plav_1521 OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=Plav_1521 PE=3 SV=1
MM1 pKa = 6.53 TTRR4 pKa = 11.84 HH5 pKa = 6.0 SKK7 pKa = 10.87 NITGTTGAIDD17 pKa = 3.58 SSLLGSAYY25 pKa = 10.15 SYY27 pKa = 11.25 YY28 pKa = 10.94 GGVVQTAHH36 pKa = 6.75 SSGKK40 pKa = 7.27 TASGVKK46 pKa = 9.76 RR47 pKa = 11.84 VFAAAMLSGASVLAVGIAGTGNAAAGTCVGPINEE81 pKa = 4.91 TYY83 pKa = 9.52 TCAGTFDD90 pKa = 3.9 DD91 pKa = 4.81 TIEE94 pKa = 4.24 YY95 pKa = 10.57 DD96 pKa = 3.56 SGVDD100 pKa = 4.48 DD101 pKa = 3.77 VTLVLGPGSFIDD113 pKa = 3.62 TTEE116 pKa = 3.86 NTSEE120 pKa = 4.43 HH121 pKa = 6.49 DD122 pKa = 3.46 HH123 pKa = 7.11 DD124 pKa = 3.99 NAGIVVVGEE133 pKa = 3.82 SDD135 pKa = 3.34 MAIVNNGSIFTGDD148 pKa = 2.73 NTTWVEE154 pKa = 3.59 TDD156 pKa = 3.15 EE157 pKa = 4.59 EE158 pKa = 4.44 YY159 pKa = 10.99 GYY161 pKa = 10.88 YY162 pKa = 10.42 AYY164 pKa = 10.88 LGDD167 pKa = 3.89 GHH169 pKa = 8.13 HH170 pKa = 7.29 GIAAYY175 pKa = 10.68 SNWDD179 pKa = 3.45 DD180 pKa = 3.74 AAVEE184 pKa = 4.06 NTAFGTIVTTSSEE197 pKa = 4.21 SHH199 pKa = 5.85 GMVAEE204 pKa = 4.1 ALEE207 pKa = 4.94 GSASADD213 pKa = 3.29 NAGLIGTSGEE223 pKa = 4.13 DD224 pKa = 3.1 SHH226 pKa = 7.73 GISATAKK233 pKa = 8.98 YY234 pKa = 10.52 SVDD237 pKa = 3.37 VVNSGTITTDD247 pKa = 2.61 GTYY250 pKa = 10.75 SHH252 pKa = 7.26 GIWAALDD259 pKa = 3.4 SEE261 pKa = 4.52 YY262 pKa = 10.93 GYY264 pKa = 11.47 GSFGGIIEE272 pKa = 4.34 ISNSGSIEE280 pKa = 4.02 TSGEE284 pKa = 4.13 SASGISANAEE294 pKa = 3.89 EE295 pKa = 5.1 GDD297 pKa = 3.93 VEE299 pKa = 4.31 IANSGSIVTEE309 pKa = 3.78 GDD311 pKa = 3.05 EE312 pKa = 4.93 ALGIWAHH319 pKa = 6.44 GDD321 pKa = 3.43 DD322 pKa = 3.74 VDD324 pKa = 5.16 VVNTADD330 pKa = 3.89 GSIVTYY336 pKa = 10.26 GADD339 pKa = 2.93 AHH341 pKa = 6.06 GVYY344 pKa = 10.3 IFGDD348 pKa = 3.87 TVSLSNAGTIEE359 pKa = 4.23 TYY361 pKa = 10.98 GEE363 pKa = 3.92 DD364 pKa = 3.02 AHH366 pKa = 8.29 AVVAYY371 pKa = 10.3 SGGIDD376 pKa = 3.14 TTTIVNTGLIQATGEE391 pKa = 3.82 EE392 pKa = 4.17 ADD394 pKa = 4.7 AIRR397 pKa = 11.84 ASGPTVRR404 pKa = 11.84 ITNDD408 pKa = 3.01 VIEE411 pKa = 4.51 EE412 pKa = 4.12 DD413 pKa = 4.35 EE414 pKa = 4.32 EE415 pKa = 4.57 VVDD418 pKa = 3.78 YY419 pKa = 11.18 GVIRR423 pKa = 11.84 SEE425 pKa = 4.34 DD426 pKa = 3.2 GAAIHH431 pKa = 5.6 VTEE434 pKa = 5.22 ADD436 pKa = 4.07 DD437 pKa = 4.5 ARR439 pKa = 11.84 LYY441 pKa = 11.41 NNGAIYY447 pKa = 10.58 GNVSIEE453 pKa = 3.94 TDD455 pKa = 3.2 EE456 pKa = 4.04 YY457 pKa = 11.09 GYY459 pKa = 11.42 AVNTGSISSDD469 pKa = 3.56 RR470 pKa = 11.84 KK471 pKa = 8.89 WKK473 pKa = 10.26 AALAIDD479 pKa = 4.14 AEE481 pKa = 4.41 EE482 pKa = 5.05 GDD484 pKa = 4.02 VVVVNDD490 pKa = 4.19 GDD492 pKa = 3.89 IEE494 pKa = 4.4 ASGRR498 pKa = 11.84 KK499 pKa = 9.17 AKK501 pKa = 10.35 GVEE504 pKa = 3.84 LEE506 pKa = 4.76 GYY508 pKa = 10.32 DD509 pKa = 3.51 VTLTNTGSIEE519 pKa = 4.0 TWGSDD524 pKa = 2.74 GHH526 pKa = 7.82 AVDD529 pKa = 5.38 LEE531 pKa = 4.05 GDD533 pKa = 3.67 EE534 pKa = 4.91 EE535 pKa = 4.52 YY536 pKa = 11.63 GEE538 pKa = 4.31 VTLNNSGLIQASGEE552 pKa = 3.9 EE553 pKa = 4.04 ADD555 pKa = 3.81 AVRR558 pKa = 11.84 ASGEE562 pKa = 4.23 TVRR565 pKa = 11.84 ITNQAVLVPGEE576 pKa = 3.91 TDD578 pKa = 3.64 AYY580 pKa = 9.93 DD581 pKa = 3.35 TTIYY585 pKa = 10.69 GIIRR589 pKa = 11.84 SEE591 pKa = 4.17 DD592 pKa = 3.16 GAAIRR597 pKa = 11.84 VDD599 pKa = 3.64 EE600 pKa = 4.44 SDD602 pKa = 3.18 VVYY605 pKa = 10.79 VVNDD609 pKa = 3.17 GFIYY613 pKa = 10.72 SNISIEE619 pKa = 4.33 ADD621 pKa = 3.19 DD622 pKa = 3.81 YY623 pKa = 11.83 AYY625 pKa = 11.1 VSNAGSVSSDD635 pKa = 3.44 RR636 pKa = 11.84 RR637 pKa = 11.84 WKK639 pKa = 10.41 AAVSADD645 pKa = 3.67 VEE647 pKa = 4.39 EE648 pKa = 4.96 GNAVIVNDD656 pKa = 4.22 GEE658 pKa = 4.49 IVTTASRR665 pKa = 11.84 AAGIEE670 pKa = 3.9 VDD672 pKa = 4.03 VEE674 pKa = 4.07 LGDD677 pKa = 4.07 AYY679 pKa = 11.11 VVNSGTITTGSLEE692 pKa = 4.04 EE693 pKa = 4.61 GEE695 pKa = 4.81 PEE697 pKa = 4.56 GGWRR701 pKa = 11.84 SHH703 pKa = 6.74 GIDD706 pKa = 3.44 VFAEE710 pKa = 4.03 DD711 pKa = 3.99 TVLVLNTGDD720 pKa = 3.33 VTTYY724 pKa = 8.85 GHH726 pKa = 6.64 KK727 pKa = 10.74 AKK729 pKa = 10.61 GIRR732 pKa = 11.84 IEE734 pKa = 4.3 TQEE737 pKa = 3.74 GTAAGVNSGTIDD749 pKa = 2.92 TWGEE753 pKa = 3.86 DD754 pKa = 3.42 STGLIVTATSRR765 pKa = 11.84 EE766 pKa = 4.23 HH767 pKa = 6.32 YY768 pKa = 10.29 DD769 pKa = 3.27 GYY771 pKa = 11.28 SYY773 pKa = 11.57 FDD775 pKa = 3.12 ISGIALAGNIGSVEE789 pKa = 4.07 TRR791 pKa = 11.84 GDD793 pKa = 3.4 DD794 pKa = 3.36 AMGVVAVAEE803 pKa = 4.64 GNFAAALNVLGGSISTSGDD822 pKa = 3.37 DD823 pKa = 3.37 AHH825 pKa = 7.63 GLVAVSGFEE834 pKa = 4.15 SVEE837 pKa = 3.99 DD838 pKa = 3.65 ALEE841 pKa = 4.2 GSEE844 pKa = 3.97 YY845 pKa = 10.98 SEE847 pKa = 4.64 GGEE850 pKa = 3.92 GSYY853 pKa = 10.9 AIAVNGLPPQLLAGFSGIGTEE874 pKa = 4.1 MGSGVLGEE882 pKa = 4.29 IADD885 pKa = 4.14 VLAEE889 pKa = 4.15 ALPEE893 pKa = 4.27 GEE895 pKa = 4.55 YY896 pKa = 11.11 DD897 pKa = 3.58 PADD900 pKa = 3.62 FRR902 pKa = 11.84 STIVTTGDD910 pKa = 3.18 GAIGVLAVSSNGGAVAGNLYY930 pKa = 10.48 GDD932 pKa = 3.81 VVTGVLHH939 pKa = 6.16 EE940 pKa = 4.66 EE941 pKa = 5.26 GYY943 pKa = 10.78 SVGGDD948 pKa = 3.28 HH949 pKa = 7.46 AYY951 pKa = 10.22 GVAAMSVDD959 pKa = 3.42 EE960 pKa = 4.88 GGAIAFNAYY969 pKa = 9.23 NASIVTNGAYY979 pKa = 10.18 AHH981 pKa = 6.65 GLLAISEE988 pKa = 5.08 DD989 pKa = 3.8 GDD991 pKa = 3.56 AMAMNKK997 pKa = 9.37 YY998 pKa = 10.26 ASSIVTNGLGAVGIRR1013 pKa = 11.84 VWTDD1017 pKa = 3.27 GEE1019 pKa = 4.07 SDD1021 pKa = 3.85 EE1022 pKa = 4.87 YY1023 pKa = 11.61 SSADD1027 pKa = 3.28 ALVWNVGSSVTTYY1040 pKa = 10.72 GQGAQGIVAFSEE1052 pKa = 4.17 EE1053 pKa = 4.25 GDD1055 pKa = 3.72 VTVANAPMTIGEE1067 pKa = 4.55 GEE1069 pKa = 4.33 DD1070 pKa = 3.53 EE1071 pKa = 4.97 EE1072 pKa = 4.65 ILAGLIEE1079 pKa = 4.29 THH1081 pKa = 6.64 GDD1083 pKa = 3.28 EE1084 pKa = 5.88 ADD1086 pKa = 4.67 GIWASGEE1093 pKa = 4.06 SDD1095 pKa = 3.04 VDD1097 pKa = 4.07 VYY1099 pKa = 11.82 NSGSIITHH1107 pKa = 5.56 GEE1109 pKa = 3.83 GANGISASGDD1119 pKa = 3.3 EE1120 pKa = 4.07 VDD1122 pKa = 4.55 IDD1124 pKa = 3.81 NSGTIQTSGDD1134 pKa = 3.37 EE1135 pKa = 4.16 AFGVDD1140 pKa = 3.36 MRR1142 pKa = 11.84 GWSVSLDD1149 pKa = 3.4 NSGTISTSGKK1159 pKa = 8.38 YY1160 pKa = 9.2 AHH1162 pKa = 6.92 AVVGDD1167 pKa = 4.17 SYY1169 pKa = 12.15 SDD1171 pKa = 3.46 LTTTVYY1177 pKa = 11.08 NSGTIEE1183 pKa = 4.04 ATGKK1187 pKa = 9.1 YY1188 pKa = 9.47 ANAVRR1193 pKa = 11.84 VSGPTVHH1200 pKa = 5.76 VTNTEE1205 pKa = 4.02 DD1206 pKa = 3.71 GVISSGDD1213 pKa = 3.28 VAIYY1217 pKa = 10.62 AFEE1220 pKa = 4.08 TKK1222 pKa = 9.91 YY1223 pKa = 11.42 ASIANHH1229 pKa = 6.26 GEE1231 pKa = 4.17 VEE1233 pKa = 4.29 GSIHH1237 pKa = 6.13 VSAYY1241 pKa = 10.32 DD1242 pKa = 3.73 YY1243 pKa = 11.55 EE1244 pKa = 5.65 DD1245 pKa = 3.57 FASTYY1250 pKa = 9.94 IVNTGSVSGDD1260 pKa = 2.56 IDD1262 pKa = 3.76 TSFGEE1267 pKa = 4.08 SDD1269 pKa = 4.95 DD1270 pKa = 4.46 IIIVDD1275 pKa = 4.08 GGTVGGAIFTGDD1287 pKa = 3.59 GEE1289 pKa = 4.79 DD1290 pKa = 3.49 EE1291 pKa = 4.3 VTISGTGVSIAKK1303 pKa = 9.79 GIHH1306 pKa = 6.21 GDD1308 pKa = 3.88 GEE1310 pKa = 4.65 GDD1312 pKa = 3.4 LHH1314 pKa = 8.15 VLFEE1318 pKa = 4.31 QDD1320 pKa = 3.25 DD1321 pKa = 4.39 TVTFSDD1327 pKa = 4.85 GIEE1330 pKa = 4.63 GYY1332 pKa = 10.5 AISNAHH1338 pKa = 5.75 FVGFSSGTTVFDD1350 pKa = 3.58 GVNIHH1355 pKa = 5.67 TEE1357 pKa = 3.72 EE1358 pKa = 5.47 GEE1360 pKa = 4.07 ILVGEE1365 pKa = 4.59 DD1366 pKa = 2.86 ATLATTIEE1374 pKa = 4.14 NAFAFADD1381 pKa = 3.87 EE1382 pKa = 4.34 TSVLGRR1388 pKa = 11.84 LKK1390 pKa = 10.7 VVAGSSFGFSGDD1402 pKa = 3.55 VVFDD1406 pKa = 3.57 EE1407 pKa = 5.38 GGTFEE1412 pKa = 4.77 TGLTGDD1418 pKa = 3.63 GAGVVTGDD1426 pKa = 3.51 TVRR1429 pKa = 11.84 FASGSTIHH1437 pKa = 6.48 VDD1439 pKa = 3.02 AGAGFTEE1446 pKa = 4.79 TVGSDD1451 pKa = 3.24 FLIASSEE1458 pKa = 4.33 SEE1460 pKa = 4.44 SGVTDD1465 pKa = 4.24 DD1466 pKa = 5.08 GASVTDD1472 pKa = 3.58 NLILFKK1478 pKa = 10.45 FLKK1481 pKa = 10.28 VMNGDD1486 pKa = 3.76 VVSEE1490 pKa = 4.41 GAADD1494 pKa = 3.78 EE1495 pKa = 4.3 LFLRR1499 pKa = 11.84 IEE1501 pKa = 4.19 VEE1503 pKa = 3.93 EE1504 pKa = 4.18 TAFDD1508 pKa = 4.78 LEE1510 pKa = 4.63 TEE1512 pKa = 4.3 AKK1514 pKa = 9.14 GTGNLLSVASALDD1527 pKa = 3.82 VYY1529 pKa = 11.07 IEE1531 pKa = 4.21 TQPLDD1536 pKa = 3.78 NPLVQYY1542 pKa = 10.64 LLQFEE1547 pKa = 4.66 TEE1549 pKa = 4.26 EE1550 pKa = 4.07 EE1551 pKa = 3.88 QLAALLKK1558 pKa = 10.11 VIKK1561 pKa = 9.66 DD1562 pKa = 3.85 TVPDD1566 pKa = 3.87 EE1567 pKa = 4.83 SNASAGATIASTDD1580 pKa = 4.46 LIYY1583 pKa = 11.38 DD1584 pKa = 4.05 MIMDD1588 pKa = 4.84 RR1589 pKa = 11.84 LSGGGFVVADD1599 pKa = 3.89 GGMTGLSAGDD1609 pKa = 3.56 AVLGGDD1615 pKa = 4.74 GNWALWGRR1623 pKa = 11.84 VGGSKK1628 pKa = 10.76 ASFTPSGVNGFDD1640 pKa = 3.4 ADD1642 pKa = 3.37 SWGVSLGLDD1651 pKa = 3.67 GEE1653 pKa = 4.61 VAPALRR1659 pKa = 11.84 LGFGGFYY1666 pKa = 10.04 IASDD1670 pKa = 3.82 VEE1672 pKa = 4.49 EE1673 pKa = 4.65 NGAGANSTNDD1683 pKa = 2.81 IAGYY1687 pKa = 10.9 GFTTYY1692 pKa = 10.15 MSWRR1696 pKa = 11.84 PGAWYY1701 pKa = 10.9 VNGALGYY1708 pKa = 11.3 GMNTYY1713 pKa = 10.96 DD1714 pKa = 4.89 SRR1716 pKa = 11.84 RR1717 pKa = 11.84 TSLGSVNTADD1727 pKa = 3.56 YY1728 pKa = 11.38 DD1729 pKa = 3.72 GTQFVARR1736 pKa = 11.84 AEE1738 pKa = 4.03 AGYY1741 pKa = 9.13 MIVSGQWDD1749 pKa = 3.78 LTPNVGLRR1757 pKa = 11.84 YY1758 pKa = 10.03 NRR1760 pKa = 11.84 VDD1762 pKa = 2.89 IDD1764 pKa = 4.21 GYY1766 pKa = 11.54 AEE1768 pKa = 4.27 TGPLPISVNSQTVDD1782 pKa = 2.8 SLRR1785 pKa = 11.84 AVAGVNARR1793 pKa = 11.84 YY1794 pKa = 7.84 TMPLDD1799 pKa = 3.96 GGGKK1803 pKa = 10.15 LIPEE1807 pKa = 4.88 FGVKK1811 pKa = 10.33 LLGEE1815 pKa = 4.28 LADD1818 pKa = 4.25 PDD1820 pKa = 4.16 GAITGQIVGGGVFVTQTTPRR1840 pKa = 11.84 DD1841 pKa = 4.18 DD1842 pKa = 3.04 ISYY1845 pKa = 10.78 GVGTGITYY1853 pKa = 7.98 EE1854 pKa = 4.2 ASDD1857 pKa = 3.62 KK1858 pKa = 10.85 FSIRR1862 pKa = 11.84 VTYY1865 pKa = 10.53 DD1866 pKa = 3.04 GEE1868 pKa = 4.22 FQSDD1872 pKa = 3.63 YY1873 pKa = 11.63 DD1874 pKa = 4.05 EE1875 pKa = 4.6 QALSAAIRR1883 pKa = 11.84 WAFF1886 pKa = 3.13
Molecular weight: 193.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.554
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.884
Patrickios 0.947
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A7HPY6|A7HPY6_PARL1 Copper-resistance protein CopA family OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=Plav_0346 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.58 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 TQTVGGRR28 pKa = 11.84 KK29 pKa = 8.9 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.53 GRR39 pKa = 11.84 KK40 pKa = 8.77 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3580
0
3580
1152854
29
3325
322.0
35.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.31 ± 0.058
0.811 ± 0.012
5.651 ± 0.031
6.518 ± 0.038
3.815 ± 0.027
8.689 ± 0.039
2.057 ± 0.018
5.15 ± 0.032
3.625 ± 0.035
9.87 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.594 ± 0.019
2.636 ± 0.023
5.112 ± 0.03
2.808 ± 0.022
7.026 ± 0.041
5.249 ± 0.032
5.167 ± 0.038
7.189 ± 0.032
1.353 ± 0.018
2.372 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here