Amino acid dipepetide frequency for Ruminococcus sp. CAG:330

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.53AlaAla: 10.53 ± 0.198	1.551AlaCys: 1.551 ± 0.046	5.686AlaAsp: 5.686 ± 0.09	6.756AlaGlu: 6.756 ± 0.126	3.482AlaPhe: 3.482 ± 0.094	6.831AlaGly: 6.831 ± 0.129	1.452AlaHis: 1.452 ± 0.059	4.998AlaIle: 4.998 ± 0.088	4.714AlaLys: 4.714 ± 0.093	8.168AlaLeu: 8.168 ± 0.119	2.819AlaMet: 2.819 ± 0.07	2.543AlaAsn: 2.543 ± 0.075	2.807AlaPro: 2.807 ± 0.074	3.619AlaGln: 3.619 ± 0.086	3.174AlaArg: 3.174 ± 0.081	4.687AlaSer: 4.687 ± 0.091	3.985AlaThr: 3.985 ± 0.105	7.755AlaVal: 7.755 ± 0.143	0.755AlaTrp: 0.755 ± 0.034	3.161AlaTyr: 3.161 ± 0.072	0.001AlaXaa: 0.001 ± 0.001
Cys	1.698CysAla: 1.698 ± 0.05	0.45CysCys: 0.45 ± 0.028	1.04CysAsp: 1.04 ± 0.042	1.0CysGlu: 1.0 ± 0.043	0.729CysPhe: 0.729 ± 0.038	1.989CysGly: 1.989 ± 0.066	0.457CysHis: 0.457 ± 0.027	1.127CysIle: 1.127 ± 0.039	0.781CysLys: 0.781 ± 0.038	1.426CysLeu: 1.426 ± 0.061	0.452CysMet: 0.452 ± 0.026	0.643CysAsn: 0.643 ± 0.031	0.831CysPro: 0.831 ± 0.039	0.653CysGln: 0.653 ± 0.037	1.087CysArg: 1.087 ± 0.043	1.255CysSer: 1.255 ± 0.051	1.188CysThr: 1.188 ± 0.045	1.154CysVal: 1.154 ± 0.04	0.174CysTrp: 0.174 ± 0.015	0.727CysTyr: 0.727 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.643AspAla: 5.643 ± 0.097	1.143AspCys: 1.143 ± 0.038	3.119AspAsp: 3.119 ± 0.085	3.471AspGlu: 3.471 ± 0.084	2.435AspPhe: 2.435 ± 0.069	5.328AspGly: 5.328 ± 0.11	0.875AspHis: 0.875 ± 0.038	3.671AspIle: 3.671 ± 0.079	2.556AspLys: 2.556 ± 0.062	4.531AspLeu: 4.531 ± 0.084	1.647AspMet: 1.647 ± 0.053	1.912AspAsn: 1.912 ± 0.05	1.868AspPro: 1.868 ± 0.063	1.383AspGln: 1.383 ± 0.049	2.291AspArg: 2.291 ± 0.054	3.696AspSer: 3.696 ± 0.087	3.657AspThr: 3.657 ± 0.077	4.067AspVal: 4.067 ± 0.069	0.63AspTrp: 0.63 ± 0.033	2.513AspTyr: 2.513 ± 0.068	0.001AspXaa: 0.001 ± 0.002
Glu	5.449GluAla: 5.449 ± 0.117	0.805GluCys: 0.805 ± 0.029	3.624GluAsp: 3.624 ± 0.082	5.452GluGlu: 5.452 ± 0.122	2.127GluPhe: 2.127 ± 0.058	3.422GluGly: 3.422 ± 0.073	1.453GluHis: 1.453 ± 0.048	4.777GluIle: 4.777 ± 0.092	5.19GluLys: 5.19 ± 0.125	5.935GluLeu: 5.935 ± 0.11	2.235GluMet: 2.235 ± 0.057	3.629GluAsn: 3.629 ± 0.069	2.043GluPro: 2.043 ± 0.074	3.55GluGln: 3.55 ± 0.079	3.271GluArg: 3.271 ± 0.081	3.664GluSer: 3.664 ± 0.091	4.338GluThr: 4.338 ± 0.096	3.848GluVal: 3.848 ± 0.082	0.547GluTrp: 0.547 ± 0.031	2.328GluTyr: 2.328 ± 0.065	0.0GluXaa: 0.0 ± 0.0
Phe	3.337PheAla: 3.337 ± 0.07	1.013PheCys: 1.013 ± 0.042	2.341PheAsp: 2.341 ± 0.061	2.049PheGlu: 2.049 ± 0.056	1.734PhePhe: 1.734 ± 0.059	3.037PheGly: 3.037 ± 0.069	1.017PheHis: 1.017 ± 0.039	1.724PheIle: 1.724 ± 0.062	1.064PheLys: 1.064 ± 0.043	4.095PheLeu: 4.095 ± 0.092	0.805PheMet: 0.805 ± 0.036	1.108PheAsn: 1.108 ± 0.048	1.617PhePro: 1.617 ± 0.058	1.772PheGln: 1.772 ± 0.05	2.247PheArg: 2.247 ± 0.056	3.247PheSer: 3.247 ± 0.075	2.208PheThr: 2.208 ± 0.058	2.483PheVal: 2.483 ± 0.06	0.417PheTrp: 0.417 ± 0.029	1.741PheTyr: 1.741 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	5.639GlyAla: 5.639 ± 0.116	1.593GlyCys: 1.593 ± 0.065	4.146GlyAsp: 4.146 ± 0.087	4.505GlyGlu: 4.505 ± 0.102	2.764GlyPhe: 2.764 ± 0.064	5.229GlyGly: 5.229 ± 0.117	1.238GlyHis: 1.238 ± 0.058	5.428GlyIle: 5.428 ± 0.108	5.051GlyLys: 5.051 ± 0.1	5.522GlyLeu: 5.522 ± 0.108	2.415GlyMet: 2.415 ± 0.062	3.032GlyAsn: 3.032 ± 0.082	1.08GlyPro: 1.08 ± 0.047	2.047GlyGln: 2.047 ± 0.054	2.842GlyArg: 2.842 ± 0.073	4.251GlySer: 4.251 ± 0.083	5.039GlyThr: 5.039 ± 0.101	5.541GlyVal: 5.541 ± 0.095	0.745GlyTrp: 0.745 ± 0.044	3.127GlyTyr: 3.127 ± 0.077	0.006GlyXaa: 0.006 ± 0.003
His	1.685HisAla: 1.685 ± 0.051	0.453HisCys: 0.453 ± 0.029	1.0HisAsp: 1.0 ± 0.038	1.009HisGlu: 1.009 ± 0.036	0.94HisPhe: 0.94 ± 0.041	1.693HisGly: 1.693 ± 0.058	0.529HisHis: 0.529 ± 0.03	1.324HisIle: 1.324 ± 0.044	0.823HisLys: 0.823 ± 0.036	1.875HisLeu: 1.875 ± 0.054	0.557HisMet: 0.557 ± 0.027	0.729HisAsn: 0.729 ± 0.032	1.037HisPro: 1.037 ± 0.042	0.742HisGln: 0.742 ± 0.039	1.086HisArg: 1.086 ± 0.041	1.113HisSer: 1.113 ± 0.044	1.2HisThr: 1.2 ± 0.042	1.356HisVal: 1.356 ± 0.049	0.245HisTrp: 0.245 ± 0.018	0.729HisTyr: 0.729 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.908IleAla: 5.908 ± 0.096	1.294IleCys: 1.294 ± 0.041	3.404IleAsp: 3.404 ± 0.07	3.231IleGlu: 3.231 ± 0.081	2.435IlePhe: 2.435 ± 0.08	4.649IleGly: 4.649 ± 0.116	1.257IleHis: 1.257 ± 0.046	3.287IleIle: 3.287 ± 0.089	2.067IleLys: 2.067 ± 0.062	6.173IleLeu: 6.173 ± 0.113	1.324IleMet: 1.324 ± 0.05	1.859IleAsn: 1.859 ± 0.054	3.322IlePro: 3.322 ± 0.083	2.56IleGln: 2.56 ± 0.056	3.61IleArg: 3.61 ± 0.079	4.485IleSer: 4.485 ± 0.086	3.889IleThr: 3.889 ± 0.079	4.186IleVal: 4.186 ± 0.092	0.621IleTrp: 0.621 ± 0.03	2.173IleTyr: 2.173 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.187LysAla: 4.187 ± 0.084	0.712LysCys: 0.712 ± 0.038	2.737LysAsp: 2.737 ± 0.064	3.701LysGlu: 3.701 ± 0.085	1.681LysPhe: 1.681 ± 0.055	3.025LysGly: 3.025 ± 0.061	1.02LysHis: 1.02 ± 0.037	3.565LysIle: 3.565 ± 0.081	4.317LysLys: 4.317 ± 0.099	4.796LysLeu: 4.796 ± 0.108	1.777LysMet: 1.777 ± 0.048	2.586LysAsn: 2.586 ± 0.059	2.1LysPro: 2.1 ± 0.061	2.275LysGln: 2.275 ± 0.066	2.812LysArg: 2.812 ± 0.077	2.909LysSer: 2.909 ± 0.068	3.307LysThr: 3.307 ± 0.081	2.881LysVal: 2.881 ± 0.069	0.541LysTrp: 0.541 ± 0.028	2.059LysTyr: 2.059 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	7.602LeuAla: 7.602 ± 0.123	2.11LeuCys: 2.11 ± 0.069	4.984LeuAsp: 4.984 ± 0.08	6.395LeuGlu: 6.395 ± 0.123	3.58LeuPhe: 3.58 ± 0.104	5.969LeuGly: 5.969 ± 0.114	2.16LeuHis: 2.16 ± 0.067	5.049LeuIle: 5.049 ± 0.121	4.492LeuLys: 4.492 ± 0.094	9.686LeuLeu: 9.686 ± 0.194	2.374LeuMet: 2.374 ± 0.058	3.01LeuAsn: 3.01 ± 0.062	4.29LeuPro: 4.29 ± 0.085	3.926LeuGln: 3.926 ± 0.087	4.956LeuArg: 4.956 ± 0.099	6.246LeuSer: 6.246 ± 0.113	5.551LeuThr: 5.551 ± 0.098	5.553LeuVal: 5.553 ± 0.11	0.852LeuTrp: 0.852 ± 0.044	3.24LeuTyr: 3.24 ± 0.069	0.004LeuXaa: 0.004 ± 0.003
Met	2.532MetAla: 2.532 ± 0.061	0.37MetCys: 0.37 ± 0.024	1.529MetAsp: 1.529 ± 0.047	2.166MetGlu: 2.166 ± 0.065	0.898MetPhe: 0.898 ± 0.035	1.922MetGly: 1.922 ± 0.062	0.598MetHis: 0.598 ± 0.023	1.712MetIle: 1.712 ± 0.052	1.842MetLys: 1.842 ± 0.054	2.906MetLeu: 2.906 ± 0.075	0.786MetMet: 0.786 ± 0.034	1.108MetAsn: 1.108 ± 0.042	1.187MetPro: 1.187 ± 0.039	1.54MetGln: 1.54 ± 0.046	1.463MetArg: 1.463 ± 0.054	1.412MetSer: 1.412 ± 0.046	1.871MetThr: 1.871 ± 0.055	1.624MetVal: 1.624 ± 0.05	0.217MetTrp: 0.217 ± 0.016	0.831MetTyr: 0.831 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	3.526AsnAla: 3.526 ± 0.073	0.775AsnCys: 0.775 ± 0.041	1.919AsnAsp: 1.919 ± 0.056	2.007AsnGlu: 2.007 ± 0.051	1.409AsnPhe: 1.409 ± 0.047	3.489AsnGly: 3.489 ± 0.09	0.778AsnHis: 0.778 ± 0.033	2.339AsnIle: 2.339 ± 0.062	1.473AsnLys: 1.473 ± 0.05	3.364AsnLeu: 3.364 ± 0.071	1.031AsnMet: 1.031 ± 0.038	1.321AsnAsn: 1.321 ± 0.058	2.022AsnPro: 2.022 ± 0.067	1.349AsnGln: 1.349 ± 0.041	1.92AsnArg: 1.92 ± 0.054	2.107AsnSer: 2.107 ± 0.06	2.109AsnThr: 2.109 ± 0.06	2.479AsnVal: 2.479 ± 0.059	0.426AsnTrp: 0.426 ± 0.029	1.524AsnTyr: 1.524 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	3.781ProAla: 3.781 ± 0.083	0.615ProCys: 0.615 ± 0.031	2.734ProAsp: 2.734 ± 0.067	4.147ProGlu: 4.147 ± 0.086	1.571ProPhe: 1.571 ± 0.053	1.685ProGly: 1.685 ± 0.053	0.747ProHis: 0.747 ± 0.031	2.005ProIle: 2.005 ± 0.05	1.841ProLys: 1.841 ± 0.062	3.113ProLeu: 3.113 ± 0.075	1.063ProMet: 1.063 ± 0.045	1.425ProAsn: 1.425 ± 0.048	1.167ProPro: 1.167 ± 0.054	1.563ProGln: 1.563 ± 0.055	1.304ProArg: 1.304 ± 0.047	1.972ProSer: 1.972 ± 0.063	1.841ProThr: 1.841 ± 0.055	3.214ProVal: 3.214 ± 0.069	0.345ProTrp: 0.345 ± 0.022	1.453ProTyr: 1.453 ± 0.05	0.001ProXaa: 0.001 ± 0.001
Gln	3.18GlnAla: 3.18 ± 0.082	0.584GlnCys: 0.584 ± 0.029	1.869GlnAsp: 1.869 ± 0.058	3.15GlnGlu: 3.15 ± 0.084	1.584GlnPhe: 1.584 ± 0.05	2.05GlnGly: 2.05 ± 0.063	0.812GlnHis: 0.812 ± 0.037	2.744GlnIle: 2.744 ± 0.065	2.915GlnLys: 2.915 ± 0.075	3.845GlnLeu: 3.845 ± 0.078	1.419GlnMet: 1.419 ± 0.047	1.888GlnAsn: 1.888 ± 0.058	1.472GlnPro: 1.472 ± 0.049	2.103GlnGln: 2.103 ± 0.077	2.0GlnArg: 2.0 ± 0.063	2.007GlnSer: 2.007 ± 0.051	2.453GlnThr: 2.453 ± 0.059	2.308GlnVal: 2.308 ± 0.059	0.405GlnTrp: 0.405 ± 0.026	1.644GlnTyr: 1.644 ± 0.051	0.0GlnXaa: 0.0 ± 0.0
Arg	3.463ArgAla: 3.463 ± 0.072	0.945ArgCys: 0.945 ± 0.047	2.234ArgAsp: 2.234 ± 0.054	3.666ArgGlu: 3.666 ± 0.092	1.993ArgPhe: 1.993 ± 0.057	2.527ArgGly: 2.527 ± 0.068	1.057ArgHis: 1.057 ± 0.042	3.53ArgIle: 3.53 ± 0.074	3.231ArgLys: 3.231 ± 0.068	4.233ArgLeu: 4.233 ± 0.087	1.551ArgMet: 1.551 ± 0.046	2.047ArgAsn: 2.047 ± 0.059	1.492ArgPro: 1.492 ± 0.046	2.254ArgGln: 2.254 ± 0.065	2.909ArgArg: 2.909 ± 0.085	2.762ArgSer: 2.762 ± 0.072	2.797ArgThr: 2.797 ± 0.061	2.785ArgVal: 2.785 ± 0.068	0.51ArgTrp: 0.51 ± 0.03	1.932ArgTyr: 1.932 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	5.82SerAla: 5.82 ± 0.101	1.081SerCys: 1.081 ± 0.055	3.582SerAsp: 3.582 ± 0.077	3.845SerGlu: 3.845 ± 0.081	2.44SerPhe: 2.44 ± 0.061	5.618SerGly: 5.618 ± 0.1	1.161SerHis: 1.161 ± 0.037	3.684SerIle: 3.684 ± 0.106	2.782SerLys: 2.782 ± 0.077	5.153SerLeu: 5.153 ± 0.101	1.839SerMet: 1.839 ± 0.046	2.14SerAsn: 2.14 ± 0.05	1.906SerPro: 1.906 ± 0.052	1.959SerGln: 1.959 ± 0.058	2.674SerArg: 2.674 ± 0.067	3.848SerSer: 3.848 ± 0.098	3.144SerThr: 3.144 ± 0.072	4.632SerVal: 4.632 ± 0.092	0.631SerTrp: 0.631 ± 0.03	2.431SerTyr: 2.431 ± 0.066	0.001SerXaa: 0.001 ± 0.001
Thr	6.39ThrAla: 6.39 ± 0.141	0.88ThrCys: 0.88 ± 0.038	3.528ThrAsp: 3.528 ± 0.074	4.311ThrGlu: 4.311 ± 0.088	2.314ThrPhe: 2.314 ± 0.065	4.909ThrGly: 4.909 ± 0.09	0.962ThrHis: 0.962 ± 0.034	3.801ThrIle: 3.801 ± 0.072	2.747ThrLys: 2.747 ± 0.073	5.408ThrLeu: 5.408 ± 0.096	1.556ThrMet: 1.556 ± 0.045	1.831ThrAsn: 1.831 ± 0.049	2.664ThrPro: 2.664 ± 0.067	1.959ThrGln: 1.959 ± 0.054	2.158ThrArg: 2.158 ± 0.054	3.337ThrSer: 3.337 ± 0.12	5.249ThrThr: 5.249 ± 0.484	4.921ThrVal: 4.921 ± 0.098	0.521ThrTrp: 0.521 ± 0.031	2.164ThrTyr: 2.164 ± 0.061	0.003ThrXaa: 0.003 ± 0.002
Val	5.15ValAla: 5.15 ± 0.1	1.504ValCys: 1.504 ± 0.06	3.706ValAsp: 3.706 ± 0.074	3.935ValGlu: 3.935 ± 0.079	2.724ValPhe: 2.724 ± 0.068	4.144ValGly: 4.144 ± 0.103	1.349ValHis: 1.349 ± 0.045	4.361ValIle: 4.361 ± 0.076	3.248ValLys: 3.248 ± 0.068	7.185ValLeu: 7.185 ± 0.115	1.797ValMet: 1.797 ± 0.059	2.45ValAsn: 2.45 ± 0.06	3.052ValPro: 3.052 ± 0.068	2.876ValGln: 2.876 ± 0.076	3.459ValArg: 3.459 ± 0.078	4.696ValSer: 4.696 ± 0.095	4.905ValThr: 4.905 ± 0.109	4.697ValVal: 4.697 ± 0.112	0.728ValTrp: 0.728 ± 0.033	2.348ValTyr: 2.348 ± 0.058	0.001ValXaa: 0.001 ± 0.001
Trp	0.567TrpAla: 0.567 ± 0.031	0.167TrpCys: 0.167 ± 0.016	0.607TrpAsp: 0.607 ± 0.035	0.657TrpGlu: 0.657 ± 0.034	0.45TrpPhe: 0.45 ± 0.03	0.674TrpGly: 0.674 ± 0.037	0.262TrpHis: 0.262 ± 0.02	0.718TrpIle: 0.718 ± 0.032	0.645TrpLys: 0.645 ± 0.03	0.966TrpLeu: 0.966 ± 0.041	0.346TrpMet: 0.346 ± 0.024	0.64TrpAsn: 0.64 ± 0.03	0.152TrpPro: 0.152 ± 0.017	0.456TrpGln: 0.456 ± 0.026	0.449TrpArg: 0.449 ± 0.026	0.524TrpSer: 0.524 ± 0.028	0.473TrpThr: 0.473 ± 0.027	0.472TrpVal: 0.472 ± 0.026	0.12TrpTrp: 0.12 ± 0.013	0.463TrpTyr: 0.463 ± 0.031	0.001TrpXaa: 0.001 ± 0.002
Tyr	3.385TyrAla: 3.385 ± 0.073	0.813TyrCys: 0.813 ± 0.035	2.648TyrAsp: 2.648 ± 0.061	2.237TyrGlu: 2.237 ± 0.059	1.764TyrPhe: 1.764 ± 0.053	3.043TyrGly: 3.043 ± 0.075	0.942TyrHis: 0.942 ± 0.03	1.888TyrIle: 1.888 ± 0.058	1.207TyrLys: 1.207 ± 0.039	3.65TyrLeu: 3.65 ± 0.083	0.719TyrMet: 0.719 ± 0.032	1.467TyrAsn: 1.467 ± 0.051	1.476TyrPro: 1.476 ± 0.049	1.845TyrGln: 1.845 ± 0.057	2.201TyrArg: 2.201 ± 0.058	2.153TyrSer: 2.153 ± 0.059	2.409TyrThr: 2.409 ± 0.066	2.361TyrVal: 2.361 ± 0.064	0.378TyrTrp: 0.378 ± 0.025	1.799TyrTyr: 1.799 ± 0.071	0.001TyrXaa: 0.001 ± 0.002
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.004XaaGly: 0.004 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.003XaaIle: 0.003 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.004XaaArg: 0.004 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.027XaaXaa: 0.027 ± 0.01

Statistics based on 2234 proteins (701908 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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