Kalmanozyma brasiliensis (strain GHG001) (Yeast) (Pseudozyma brasiliensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Kalmanozyma; Kalmanozyma brasiliensis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5EQP3|V5EQP3_KALBG Hemerythrin domain-containing protein OS=Kalmanozyma brasiliensis (strain GHG001) OX=1365824 GN=PSEUBRA_SCAF6g00815 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.91EE3 pKa = 5.07HH4 pKa = 7.47DD5 pKa = 4.41HH6 pKa = 7.17ASDD9 pKa = 3.44AAEE12 pKa = 4.1DD13 pKa = 4.46FYY15 pKa = 11.17IEE17 pKa = 4.15GAEE20 pKa = 4.86DD21 pKa = 3.53EE22 pKa = 4.8NEE24 pKa = 3.89FDD26 pKa = 5.05GDD28 pKa = 3.92EE29 pKa = 4.24GDD31 pKa = 3.8HH32 pKa = 6.75ANVPEE37 pKa = 4.23GVYY40 pKa = 10.25KK41 pKa = 10.47VLYY44 pKa = 10.02EE45 pKa = 3.94FDD47 pKa = 3.75PVSEE51 pKa = 4.85HH52 pKa = 6.45EE53 pKa = 4.61LAVQPGDD60 pKa = 3.55VVHH63 pKa = 6.32VVGSLEE69 pKa = 4.23GGWAIAVTNGDD80 pKa = 3.81EE81 pKa = 4.44GVKK84 pKa = 10.59GLVPATYY91 pKa = 10.29LEE93 pKa = 4.38WSAPLPEE100 pKa = 4.13

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5GTZ6|V5GTZ6_KALBG Saccharopine dehydrogenase OS=Kalmanozyma brasiliensis (strain GHG001) OX=1365824 GN=PSEUBRA_SCAF11g01172 PE=4 SV=1
MM1 pKa = 7.85PLHH4 pKa = 7.26ADD6 pKa = 3.76LCTILQNASRR16 pKa = 11.84ARR18 pKa = 11.84LRR20 pKa = 11.84SIPIASTRR28 pKa = 11.84DD29 pKa = 3.42NLSILSILLQHH40 pKa = 6.32GFVHH44 pKa = 5.69NVVRR48 pKa = 11.84GTQTGPSPGSYY59 pKa = 8.78TAASPAARR67 pKa = 11.84RR68 pKa = 11.84LWVDD72 pKa = 3.41LKK74 pKa = 10.78FRR76 pKa = 11.84PDD78 pKa = 3.6DD79 pKa = 4.06RR80 pKa = 11.84PVLEE84 pKa = 4.31SMNVVSKK91 pKa = 9.96PSRR94 pKa = 11.84KK95 pKa = 8.42LTMDD99 pKa = 3.27KK100 pKa = 11.4DD101 pKa = 3.53EE102 pKa = 5.05LLRR105 pKa = 11.84FATGRR110 pKa = 11.84RR111 pKa = 11.84AKK113 pKa = 9.78FVKK116 pKa = 9.49PLDD119 pKa = 3.48MGEE122 pKa = 4.03IGIVDD127 pKa = 3.9CGKK130 pKa = 10.17NGWWEE135 pKa = 3.65VRR137 pKa = 11.84DD138 pKa = 4.28AIRR141 pKa = 11.84RR142 pKa = 11.84GYY144 pKa = 9.93GGEE147 pKa = 3.9VVARR151 pKa = 11.84VSHH154 pKa = 5.9SRR156 pKa = 11.84PTTASAQPHH165 pKa = 6.8AIDD168 pKa = 3.43VAPCRR173 pKa = 11.84RR174 pKa = 11.84SLVLARR180 pKa = 11.84TLSKK184 pKa = 10.52KK185 pKa = 10.16RR186 pKa = 11.84SEE188 pKa = 4.18RR189 pKa = 11.84LYY191 pKa = 10.14STMFGLGSAFYY202 pKa = 10.75LILFITNALAVLSEE216 pKa = 4.15DD217 pKa = 3.2RR218 pKa = 11.84FLARR222 pKa = 11.84VGWSSHH228 pKa = 5.19ALAGGNSFDD237 pKa = 5.63QGFQSNPYY245 pKa = 8.6AAGAGGEE252 pKa = 4.15NVTIKK257 pKa = 10.81ARR259 pKa = 11.84LINLISAVRR268 pKa = 11.84TLMRR272 pKa = 11.84IPLVVINIVVVIYY285 pKa = 9.6LIVLGG290 pKa = 4.37

Molecular weight: 31.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5765

0

5765

3250758

60

5157

563.9

61.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.564 ± 0.034

0.92 ± 0.009

5.965 ± 0.026

6.005 ± 0.03

3.361 ± 0.018

7.193 ± 0.034

2.247 ± 0.013

4.143 ± 0.019

4.799 ± 0.029

8.899 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.011

3.238 ± 0.017

5.788 ± 0.031

4.077 ± 0.024

6.226 ± 0.025

9.124 ± 0.039

5.833 ± 0.018

6.042 ± 0.02

1.212 ± 0.01

2.345 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski