Amino acid dipepetide frequency for Stx1 converting phage AU6Stx1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.91AlaAla: 8.91 ± 1.002
1.307AlaCys: 1.307 ± 0.384
5.108AlaAsp: 5.108 ± 0.759
7.9AlaGlu: 7.9 ± 0.87
3.623AlaPhe: 3.623 ± 0.639
8.316AlaGly: 8.316 ± 1.198
1.663AlaHis: 1.663 ± 0.396
4.811AlaIle: 4.811 ± 0.666
4.693AlaLys: 4.693 ± 0.524
6.593AlaLeu: 6.593 ± 0.667
2.911AlaMet: 2.911 ± 0.507
2.851AlaAsn: 2.851 ± 0.411
2.732AlaPro: 2.732 ± 0.4
5.405AlaGln: 5.405 ± 0.75
5.762AlaArg: 5.762 ± 0.556
5.584AlaSer: 5.584 ± 0.536
5.227AlaThr: 5.227 ± 0.898
6.237AlaVal: 6.237 ± 0.751
1.663AlaTrp: 1.663 ± 0.286
2.138AlaTyr: 2.138 ± 0.324
0.0AlaXaa: 0.0 ± 0.0
Cys
1.069CysAla: 1.069 ± 0.26
0.297CysCys: 0.297 ± 0.139
0.416CysAsp: 0.416 ± 0.161
0.713CysGlu: 0.713 ± 0.243
0.535CysPhe: 0.535 ± 0.2
0.832CysGly: 0.832 ± 0.262
0.238CysHis: 0.238 ± 0.129
0.832CysIle: 0.832 ± 0.284
0.535CysLys: 0.535 ± 0.186
0.653CysLeu: 0.653 ± 0.208
0.297CysMet: 0.297 ± 0.122
0.356CysAsn: 0.356 ± 0.219
0.475CysPro: 0.475 ± 0.207
0.178CysGln: 0.178 ± 0.105
1.307CysArg: 1.307 ± 0.377
0.713CysSer: 0.713 ± 0.263
0.653CysThr: 0.653 ± 0.235
0.95CysVal: 0.95 ± 0.255
0.178CysTrp: 0.178 ± 0.114
0.297CysTyr: 0.297 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
5.94AspAla: 5.94 ± 0.687
0.416AspCys: 0.416 ± 0.187
3.92AspAsp: 3.92 ± 0.47
4.514AspGlu: 4.514 ± 0.565
1.604AspPhe: 1.604 ± 0.31
4.752AspGly: 4.752 ± 0.526
0.832AspHis: 0.832 ± 0.25
3.742AspIle: 3.742 ± 0.434
4.158AspLys: 4.158 ± 0.377
4.277AspLeu: 4.277 ± 0.493
1.901AspMet: 1.901 ± 0.301
2.911AspAsn: 2.911 ± 0.448
2.435AspPro: 2.435 ± 0.435
1.485AspGln: 1.485 ± 0.35
3.208AspArg: 3.208 ± 0.386
2.911AspSer: 2.911 ± 0.453
2.614AspThr: 2.614 ± 0.5
3.802AspVal: 3.802 ± 0.356
1.129AspTrp: 1.129 ± 0.315
1.485AspTyr: 1.485 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
6.475GluAla: 6.475 ± 0.693
0.832GluCys: 0.832 ± 0.247
2.138GluAsp: 2.138 ± 0.288
3.564GluGlu: 3.564 ± 0.489
2.257GluPhe: 2.257 ± 0.387
3.861GluGly: 3.861 ± 0.556
1.069GluHis: 1.069 ± 0.256
3.861GluIle: 3.861 ± 0.482
4.217GluLys: 4.217 ± 0.618
5.168GluLeu: 5.168 ± 0.63
2.079GluMet: 2.079 ± 0.331
2.911GluAsn: 2.911 ± 0.452
1.604GluPro: 1.604 ± 0.322
4.336GluGln: 4.336 ± 0.657
5.584GluArg: 5.584 ± 0.678
3.98GluSer: 3.98 ± 0.433
3.623GluThr: 3.623 ± 0.648
4.514GluVal: 4.514 ± 0.632
1.188GluTrp: 1.188 ± 0.252
1.96GluTyr: 1.96 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
2.673PheAla: 2.673 ± 0.422
0.356PheCys: 0.356 ± 0.141
1.782PheAsp: 1.782 ± 0.371
1.188PheGlu: 1.188 ± 0.339
1.307PhePhe: 1.307 ± 0.289
2.495PheGly: 2.495 ± 0.299
0.772PheHis: 0.772 ± 0.195
1.901PheIle: 1.901 ± 0.327
1.663PheLys: 1.663 ± 0.34
2.02PheLeu: 2.02 ± 0.347
0.95PheMet: 0.95 ± 0.256
1.307PheAsn: 1.307 ± 0.292
1.485PhePro: 1.485 ± 0.336
0.772PheGln: 0.772 ± 0.217
2.376PheArg: 2.376 ± 0.371
2.97PheSer: 2.97 ± 0.435
2.317PheThr: 2.317 ± 0.272
2.138PheVal: 2.138 ± 0.374
0.832PheTrp: 0.832 ± 0.225
1.129PheTyr: 1.129 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
6.356GlyAla: 6.356 ± 0.956
0.891GlyCys: 0.891 ± 0.326
4.752GlyAsp: 4.752 ± 0.895
5.821GlyGlu: 5.821 ± 1.603
2.673GlyPhe: 2.673 ± 0.454
5.405GlyGly: 5.405 ± 0.766
1.01GlyHis: 1.01 ± 0.253
3.92GlyIle: 3.92 ± 0.677
4.514GlyLys: 4.514 ± 0.889
5.287GlyLeu: 5.287 ± 0.493
2.554GlyMet: 2.554 ± 0.377
2.614GlyAsn: 2.614 ± 0.339
4.099GlyPro: 4.099 ± 2.586
2.732GlyGln: 2.732 ± 0.439
4.396GlyArg: 4.396 ± 0.601
3.742GlySer: 3.742 ± 0.532
3.683GlyThr: 3.683 ± 0.518
5.524GlyVal: 5.524 ± 0.53
1.188GlyTrp: 1.188 ± 0.298
2.317GlyTyr: 2.317 ± 0.408
0.0GlyXaa: 0.0 ± 0.0
His
1.604HisAla: 1.604 ± 0.288
0.238HisCys: 0.238 ± 0.11
1.069HisAsp: 1.069 ± 0.265
1.01HisGlu: 1.01 ± 0.261
0.891HisPhe: 0.891 ± 0.283
1.604HisGly: 1.604 ± 0.355
0.297HisHis: 0.297 ± 0.152
0.713HisIle: 0.713 ± 0.218
0.891HisLys: 0.891 ± 0.277
1.485HisLeu: 1.485 ± 0.389
0.297HisMet: 0.297 ± 0.136
1.307HisAsn: 1.307 ± 0.218
0.832HisPro: 0.832 ± 0.31
0.713HisGln: 0.713 ± 0.249
0.891HisArg: 0.891 ± 0.244
0.891HisSer: 0.891 ± 0.203
0.653HisThr: 0.653 ± 0.164
1.069HisVal: 1.069 ± 0.259
0.238HisTrp: 0.238 ± 0.139
0.713HisTyr: 0.713 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
4.336IleAla: 4.336 ± 0.469
0.95IleCys: 0.95 ± 0.225
3.92IleAsp: 3.92 ± 0.548
3.029IleGlu: 3.029 ± 0.434
1.426IlePhe: 1.426 ± 0.335
2.495IleGly: 2.495 ± 0.501
1.01IleHis: 1.01 ± 0.244
2.376IleIle: 2.376 ± 0.353
2.911IleLys: 2.911 ± 0.424
3.564IleLeu: 3.564 ± 0.697
1.366IleMet: 1.366 ± 0.282
3.386IleAsn: 3.386 ± 0.411
2.732IlePro: 2.732 ± 0.432
1.901IleGln: 1.901 ± 0.262
4.811IleArg: 4.811 ± 0.499
4.039IleSer: 4.039 ± 0.565
3.98IleThr: 3.98 ± 0.654
2.02IleVal: 2.02 ± 0.437
0.535IleTrp: 0.535 ± 0.219
1.782IleTyr: 1.782 ± 0.378
0.0IleXaa: 0.0 ± 0.0
Lys
5.821LysAla: 5.821 ± 0.661
0.535LysCys: 0.535 ± 0.231
3.029LysAsp: 3.029 ± 0.415
3.742LysGlu: 3.742 ± 0.393
1.069LysPhe: 1.069 ± 0.203
5.108LysGly: 5.108 ± 1.315
1.188LysHis: 1.188 ± 0.257
3.623LysIle: 3.623 ± 0.452
3.92LysLys: 3.92 ± 0.56
4.752LysLeu: 4.752 ± 0.569
1.901LysMet: 1.901 ± 0.371
3.148LysAsn: 3.148 ± 0.359
2.911LysPro: 2.911 ± 0.531
3.029LysGln: 3.029 ± 0.5
2.911LysArg: 2.911 ± 0.406
2.97LysSer: 2.97 ± 0.365
3.92LysThr: 3.92 ± 0.621
2.97LysVal: 2.97 ± 0.486
0.772LysTrp: 0.772 ± 0.236
1.901LysTyr: 1.901 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
8.613LeuAla: 8.613 ± 0.9
1.188LeuCys: 1.188 ± 0.377
4.217LeuAsp: 4.217 ± 0.521
3.267LeuGlu: 3.267 ± 0.407
2.554LeuPhe: 2.554 ± 0.417
4.574LeuGly: 4.574 ± 0.481
1.129LeuHis: 1.129 ± 0.266
3.386LeuIle: 3.386 ± 0.491
4.752LeuLys: 4.752 ± 0.514
6.415LeuLeu: 6.415 ± 0.662
2.495LeuMet: 2.495 ± 0.436
4.99LeuAsn: 4.99 ± 0.626
3.98LeuPro: 3.98 ± 0.503
2.911LeuGln: 2.911 ± 0.737
4.693LeuArg: 4.693 ± 0.641
6.059LeuSer: 6.059 ± 0.591
5.405LeuThr: 5.405 ± 0.477
4.396LeuVal: 4.396 ± 0.489
0.594LeuTrp: 0.594 ± 0.219
2.317LeuTyr: 2.317 ± 0.471
0.0LeuXaa: 0.0 ± 0.0
Met
3.267MetAla: 3.267 ± 0.371
0.178MetCys: 0.178 ± 0.099
1.307MetAsp: 1.307 ± 0.293
1.188MetGlu: 1.188 ± 0.247
0.594MetPhe: 0.594 ± 0.185
1.426MetGly: 1.426 ± 0.314
0.416MetHis: 0.416 ± 0.153
1.069MetIle: 1.069 ± 0.209
2.317MetLys: 2.317 ± 0.38
2.317MetLeu: 2.317 ± 0.364
0.832MetMet: 0.832 ± 0.254
1.663MetAsn: 1.663 ± 0.393
1.544MetPro: 1.544 ± 0.318
1.307MetGln: 1.307 ± 0.262
2.257MetArg: 2.257 ± 0.337
2.079MetSer: 2.079 ± 0.39
2.376MetThr: 2.376 ± 0.368
1.366MetVal: 1.366 ± 0.362
0.356MetTrp: 0.356 ± 0.16
0.594MetTyr: 0.594 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
4.752AsnAla: 4.752 ± 0.721
0.297AsnCys: 0.297 ± 0.135
2.554AsnAsp: 2.554 ± 0.437
3.683AsnGlu: 3.683 ± 0.456
1.544AsnPhe: 1.544 ± 0.295
3.564AsnGly: 3.564 ± 0.342
1.01AsnHis: 1.01 ± 0.306
2.97AsnIle: 2.97 ± 0.432
2.435AsnLys: 2.435 ± 0.387
3.564AsnLeu: 3.564 ± 0.473
1.426AsnMet: 1.426 ± 0.263
2.079AsnAsn: 2.079 ± 0.383
1.841AsnPro: 1.841 ± 0.343
1.901AsnGln: 1.901 ± 0.377
2.792AsnArg: 2.792 ± 0.504
2.911AsnSer: 2.911 ± 0.514
2.079AsnThr: 2.079 ± 0.363
2.257AsnVal: 2.257 ± 0.38
0.535AsnTrp: 0.535 ± 0.163
1.188AsnTyr: 1.188 ± 0.336
0.0AsnXaa: 0.0 ± 0.0
Pro
3.386ProAla: 3.386 ± 0.576
0.356ProCys: 0.356 ± 0.154
4.217ProAsp: 4.217 ± 0.473
4.871ProGlu: 4.871 ± 0.625
1.188ProPhe: 1.188 ± 0.243
2.911ProGly: 2.911 ± 0.613
0.653ProHis: 0.653 ± 0.177
1.188ProIle: 1.188 ± 0.321
3.029ProLys: 3.029 ± 0.849
2.732ProLeu: 2.732 ± 0.408
0.535ProMet: 0.535 ± 0.17
1.307ProAsn: 1.307 ± 0.29
1.307ProPro: 1.307 ± 0.315
2.376ProGln: 2.376 ± 0.627
1.901ProArg: 1.901 ± 0.377
2.614ProSer: 2.614 ± 0.409
1.901ProThr: 1.901 ± 0.366
4.336ProVal: 4.336 ± 0.557
0.713ProTrp: 0.713 ± 0.208
1.723ProTyr: 1.723 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
4.158GlnAla: 4.158 ± 0.591
0.713GlnCys: 0.713 ± 0.234
2.257GlnAsp: 2.257 ± 0.369
3.089GlnGlu: 3.089 ± 0.486
1.604GlnPhe: 1.604 ± 0.322
3.386GlnGly: 3.386 ± 0.833
0.832GlnHis: 0.832 ± 0.224
2.02GlnIle: 2.02 ± 0.387
3.148GlnLys: 3.148 ± 0.556
3.445GlnLeu: 3.445 ± 0.438
1.069GlnMet: 1.069 ± 0.279
1.841GlnAsn: 1.841 ± 0.397
1.604GlnPro: 1.604 ± 0.311
3.564GlnGln: 3.564 ± 0.906
3.505GlnArg: 3.505 ± 0.587
2.495GlnSer: 2.495 ± 0.428
2.138GlnThr: 2.138 ± 0.347
2.792GlnVal: 2.792 ± 0.489
0.653GlnTrp: 0.653 ± 0.187
1.663GlnTyr: 1.663 ± 0.34
0.0GlnXaa: 0.0 ± 0.0
Arg
4.633ArgAla: 4.633 ± 0.461
0.356ArgCys: 0.356 ± 0.149
3.802ArgAsp: 3.802 ± 0.638
4.752ArgGlu: 4.752 ± 0.664
2.317ArgPhe: 2.317 ± 0.343
4.871ArgGly: 4.871 ± 1.019
1.604ArgHis: 1.604 ± 0.279
3.683ArgIle: 3.683 ± 0.498
3.92ArgLys: 3.92 ± 0.521
5.108ArgLeu: 5.108 ± 0.408
2.02ArgMet: 2.02 ± 0.336
3.386ArgAsn: 3.386 ± 0.427
2.079ArgPro: 2.079 ± 0.359
3.089ArgGln: 3.089 ± 0.387
5.821ArgArg: 5.821 ± 0.753
4.217ArgSer: 4.217 ± 0.566
3.802ArgThr: 3.802 ± 0.55
4.277ArgVal: 4.277 ± 0.526
1.366ArgTrp: 1.366 ± 0.272
2.257ArgTyr: 2.257 ± 0.511
0.0ArgXaa: 0.0 ± 0.0
Ser
5.881SerAla: 5.881 ± 0.74
0.535SerCys: 0.535 ± 0.207
4.039SerAsp: 4.039 ± 0.453
4.455SerGlu: 4.455 ± 0.466
1.663SerPhe: 1.663 ± 0.281
6.237SerGly: 6.237 ± 0.652
0.772SerHis: 0.772 ± 0.215
2.554SerIle: 2.554 ± 0.393
3.089SerLys: 3.089 ± 0.593
6.415SerLeu: 6.415 ± 0.684
1.663SerMet: 1.663 ± 0.433
2.376SerAsn: 2.376 ± 0.383
3.326SerPro: 3.326 ± 0.474
3.148SerGln: 3.148 ± 0.409
3.802SerArg: 3.802 ± 0.614
3.267SerSer: 3.267 ± 0.664
3.98SerThr: 3.98 ± 0.603
3.92SerVal: 3.92 ± 0.524
0.653SerTrp: 0.653 ± 0.202
1.366SerTyr: 1.366 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
5.287ThrAla: 5.287 ± 0.505
0.535ThrCys: 0.535 ± 0.227
3.267ThrAsp: 3.267 ± 0.489
3.683ThrGlu: 3.683 ± 0.471
1.723ThrPhe: 1.723 ± 0.347
5.584ThrGly: 5.584 ± 0.922
1.188ThrHis: 1.188 ± 0.24
3.683ThrIle: 3.683 ± 0.463
3.208ThrLys: 3.208 ± 0.413
5.108ThrLeu: 5.108 ± 0.618
0.832ThrMet: 0.832 ± 0.193
2.198ThrAsn: 2.198 ± 0.322
3.505ThrPro: 3.505 ± 0.364
2.198ThrGln: 2.198 ± 0.458
2.257ThrArg: 2.257 ± 0.367
4.336ThrSer: 4.336 ± 0.679
2.792ThrThr: 2.792 ± 0.368
3.742ThrVal: 3.742 ± 0.507
0.713ThrTrp: 0.713 ± 0.162
1.366ThrTyr: 1.366 ± 0.363
0.0ThrXaa: 0.0 ± 0.0
Val
6.593ValAla: 6.593 ± 0.611
0.95ValCys: 0.95 ± 0.258
3.92ValAsp: 3.92 ± 0.478
3.326ValGlu: 3.326 ± 0.424
2.079ValPhe: 2.079 ± 0.362
3.029ValGly: 3.029 ± 0.475
0.653ValHis: 0.653 ± 0.188
4.099ValIle: 4.099 ± 0.488
3.505ValLys: 3.505 ± 0.442
5.94ValLeu: 5.94 ± 0.671
1.901ValMet: 1.901 ± 0.315
2.673ValAsn: 2.673 ± 0.353
2.495ValPro: 2.495 ± 0.338
2.138ValGln: 2.138 ± 0.34
4.811ValArg: 4.811 ± 0.83
4.396ValSer: 4.396 ± 0.589
3.98ValThr: 3.98 ± 0.539
4.099ValVal: 4.099 ± 0.517
0.713ValTrp: 0.713 ± 0.17
1.901ValTyr: 1.901 ± 0.341
0.0ValXaa: 0.0 ± 0.0
Trp
0.713TrpAla: 0.713 ± 0.247
0.297TrpCys: 0.297 ± 0.149
0.594TrpAsp: 0.594 ± 0.192
0.356TrpGlu: 0.356 ± 0.129
0.713TrpPhe: 0.713 ± 0.176
0.832TrpGly: 0.832 ± 0.2
0.416TrpHis: 0.416 ± 0.169
0.713TrpIle: 0.713 ± 0.188
1.129TrpLys: 1.129 ± 0.244
1.663TrpLeu: 1.663 ± 0.38
0.535TrpMet: 0.535 ± 0.155
0.713TrpAsn: 0.713 ± 0.212
0.772TrpPro: 0.772 ± 0.292
1.247TrpGln: 1.247 ± 0.204
1.307TrpArg: 1.307 ± 0.289
1.069TrpSer: 1.069 ± 0.247
0.475TrpThr: 0.475 ± 0.199
0.832TrpVal: 0.832 ± 0.239
0.356TrpTrp: 0.356 ± 0.207
0.416TrpTyr: 0.416 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.97TyrAla: 2.97 ± 0.393
0.297TyrCys: 0.297 ± 0.144
1.723TyrAsp: 1.723 ± 0.367
1.069TyrGlu: 1.069 ± 0.292
1.069TyrPhe: 1.069 ± 0.246
2.198TyrGly: 2.198 ± 0.435
0.653TyrHis: 0.653 ± 0.198
1.426TyrIle: 1.426 ± 0.28
1.188TyrLys: 1.188 ± 0.26
1.426TyrLeu: 1.426 ± 0.32
0.832TyrMet: 0.832 ± 0.235
1.366TyrAsn: 1.366 ± 0.297
1.485TyrPro: 1.485 ± 0.276
1.604TyrGln: 1.604 ± 0.29
2.97TyrArg: 2.97 ± 0.508
1.901TyrSer: 1.901 ± 0.303
1.604TyrThr: 1.604 ± 0.436
1.96TyrVal: 1.96 ± 0.336
0.772TyrTrp: 0.772 ± 0.227
1.129TyrTyr: 1.129 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (16836 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski