Lactobacillus phage Semele

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Harbinvirus; Lactobacillus virus Semele

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VDB0|A0A2K9VDB0_9CAUD Uncharacterized protein OS=Lactobacillus phage Semele OX=2079433 PE=4 SV=1
MM1 pKa = 7.86GYY3 pKa = 8.79TDD5 pKa = 4.52GVAKK9 pKa = 10.67AMSEE13 pKa = 3.7ASAQGKK19 pKa = 9.29LFLIDD24 pKa = 3.16SFTYY28 pKa = 10.35KK29 pKa = 10.64DD30 pKa = 3.42SNYY33 pKa = 10.48NSDD36 pKa = 4.67DD37 pKa = 3.97LDD39 pKa = 4.82SEE41 pKa = 4.41ALSDD45 pKa = 5.49FLDD48 pKa = 3.39QKK50 pKa = 11.51LDD52 pKa = 3.78LDD54 pKa = 3.91STDD57 pKa = 3.56CVEE60 pKa = 4.67LLRR63 pKa = 11.84DD64 pKa = 4.12GYY66 pKa = 9.79TYY68 pKa = 10.92DD69 pKa = 3.58SEE71 pKa = 4.6GNEE74 pKa = 3.79IEE76 pKa = 4.9LVEE79 pKa = 4.42NKK81 pKa = 10.58DD82 pKa = 3.41GFTVDD87 pKa = 4.08AFQSALDD94 pKa = 4.47FIDD97 pKa = 5.55DD98 pKa = 3.8NSQGGLYY105 pKa = 10.45YY106 pKa = 10.08IFDD109 pKa = 4.03GAKK112 pKa = 9.33EE113 pKa = 3.77LLGAKK118 pKa = 9.24MGVCGEE124 pKa = 4.77HH125 pKa = 6.56IGSVSVWLDD134 pKa = 3.27TTTSQATLTQGNEE147 pKa = 4.23VYY149 pKa = 10.56HH150 pKa = 5.81LTSEE154 pKa = 4.2DD155 pKa = 3.61TNDD158 pKa = 3.03INEE161 pKa = 4.41ALNTLFYY168 pKa = 11.01DD169 pKa = 4.06

Molecular weight: 18.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VD67|A0A2K9VD67_9CAUD Uncharacterized protein OS=Lactobacillus phage Semele OX=2079433 PE=4 SV=1
MM1 pKa = 7.66LKK3 pKa = 9.59MKK5 pKa = 10.35LYY7 pKa = 10.11KK8 pKa = 10.52LLIAIVAGVTLSLFTTYY25 pKa = 10.63NASASKK31 pKa = 10.1KK32 pKa = 9.31PNYY35 pKa = 10.39AYY37 pKa = 9.68TLTRR41 pKa = 11.84AKK43 pKa = 10.11QHH45 pKa = 5.12TVKK48 pKa = 9.85LTNTGRR54 pKa = 11.84TEE56 pKa = 3.42NMYY59 pKa = 10.4RR60 pKa = 11.84VTVKK64 pKa = 10.39RR65 pKa = 11.84GKK67 pKa = 8.74KK68 pKa = 4.35TTWYY72 pKa = 10.94YY73 pKa = 9.82MALNAKK79 pKa = 9.23QSWTVKK85 pKa = 10.21QPNKK89 pKa = 10.12YY90 pKa = 8.49SVTVRR95 pKa = 11.84RR96 pKa = 11.84VSKK99 pKa = 10.81SDD101 pKa = 3.15EE102 pKa = 3.89KK103 pKa = 11.3RR104 pKa = 11.84NANPRR109 pKa = 11.84NHH111 pKa = 5.9FTPAGIKK118 pKa = 8.34NTQRR122 pKa = 11.84TVWNRR127 pKa = 2.9

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

177

0

177

40554

41

1421

229.1

25.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.002 ± 0.255

0.619 ± 0.074

7.299 ± 0.213

5.755 ± 0.232

3.467 ± 0.106

6.081 ± 0.279

1.625 ± 0.093

6.562 ± 0.166

8.031 ± 0.271

8.47 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.084

6.769 ± 0.154

2.954 ± 0.147

3.467 ± 0.139

3.445 ± 0.123

7.686 ± 0.343

6.618 ± 0.285

6.65 ± 0.146

1.013 ± 0.07

5.072 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski