Hydrogeniiclostidium mannosilyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Hydrogeniiclostidium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328UKG5|A0A328UKG5_9FIRM HTH araC/xylS-type domain-containing protein OS=Hydrogeniiclostidium mannosilyticum OX=2764322 GN=DPQ25_02840 PE=4 SV=1
MM1 pKa = 7.88FIRR4 pKa = 11.84RR5 pKa = 11.84HH6 pKa = 4.14WWAVLYY12 pKa = 8.64SVCLIAFTVYY22 pKa = 10.69LAMDD26 pKa = 3.93TFVISRR32 pKa = 11.84VYY34 pKa = 9.95TVVSEE39 pKa = 4.22TDD41 pKa = 2.81RR42 pKa = 11.84GTGSTATTDD51 pKa = 3.42TEE53 pKa = 4.07QDD55 pKa = 3.42GKK57 pKa = 11.08SDD59 pKa = 3.46SGTEE63 pKa = 4.15TVVSEE68 pKa = 4.17TSYY71 pKa = 11.49SDD73 pKa = 3.56EE74 pKa = 4.03NTQITLTEE82 pKa = 3.93YY83 pKa = 10.67RR84 pKa = 11.84EE85 pKa = 4.57CDD87 pKa = 3.07TSIYY91 pKa = 10.26VADD94 pKa = 3.7IVLSSSEE101 pKa = 3.95YY102 pKa = 10.35LQTAFAQNAYY112 pKa = 8.82GRR114 pKa = 11.84NVAEE118 pKa = 4.36KK119 pKa = 8.78TSEE122 pKa = 3.88IAEE125 pKa = 4.22SAGAILAINGDD136 pKa = 3.99YY137 pKa = 11.13YY138 pKa = 11.24GAQEE142 pKa = 4.43DD143 pKa = 5.8GYY145 pKa = 9.75VLRR148 pKa = 11.84NGVLYY153 pKa = 10.77RR154 pKa = 11.84NTAASGQEE162 pKa = 3.84DD163 pKa = 4.33LVIYY167 pKa = 10.4DD168 pKa = 5.0DD169 pKa = 4.88GSFSIINEE177 pKa = 3.97TDD179 pKa = 3.03VTAEE183 pKa = 3.91EE184 pKa = 4.28LLEE187 pKa = 6.0DD188 pKa = 4.68GAQQILSFGPALVEE202 pKa = 4.66DD203 pKa = 4.16GTVVVSEE210 pKa = 4.36DD211 pKa = 4.26DD212 pKa = 3.69EE213 pKa = 4.59VGKK216 pKa = 10.75AKK218 pKa = 10.09TSNPRR223 pKa = 11.84TAIGIIDD230 pKa = 4.14DD231 pKa = 3.71LHH233 pKa = 7.36YY234 pKa = 11.3VFVVSDD240 pKa = 3.27GRR242 pKa = 11.84TDD244 pKa = 3.29EE245 pKa = 4.52SAGLTLLQLAEE256 pKa = 4.16FMKK259 pKa = 10.75EE260 pKa = 3.91LGVTTAYY267 pKa = 10.99NLDD270 pKa = 3.92GGGSSTMYY278 pKa = 10.76FNGEE282 pKa = 4.29VINNPTTNGRR292 pKa = 11.84SIKK295 pKa = 9.64EE296 pKa = 3.84RR297 pKa = 11.84SVSDD301 pKa = 3.0IVYY304 pKa = 10.18IGYY307 pKa = 10.13

Molecular weight: 33.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328UEZ0|A0A328UEZ0_9FIRM Kinase to dihydroxyacetone kinase OS=Hydrogeniiclostidium mannosilyticum OX=2764322 GN=DPQ25_08355 PE=4 SV=1
MM1 pKa = 7.28GTRR4 pKa = 11.84KK5 pKa = 8.95PHH7 pKa = 6.0GLRR10 pKa = 11.84LPLCPYY16 pKa = 10.36CEE18 pKa = 3.97GRR20 pKa = 11.84FLYY23 pKa = 10.9GDD25 pKa = 3.61VRR27 pKa = 11.84RR28 pKa = 11.84HH29 pKa = 5.62IRR31 pKa = 11.84DD32 pKa = 3.31KK33 pKa = 11.35TGVCPNCGKK42 pKa = 10.44RR43 pKa = 11.84FIIRR47 pKa = 11.84AKK49 pKa = 10.51GRR51 pKa = 11.84IVLLWCIAGVLLCLLNLLFWSLEE74 pKa = 3.87QVNVLFIAGLTAVLLCCLFPLHH96 pKa = 6.56PFVVRR101 pKa = 11.84YY102 pKa = 9.53RR103 pKa = 11.84RR104 pKa = 11.84LQDD107 pKa = 3.53PLPPGRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84GADD118 pKa = 3.01GKK120 pKa = 11.1KK121 pKa = 10.26GGKK124 pKa = 7.81TGG126 pKa = 3.31

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2762

0

2762

860027

25

4097

311.4

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.281 ± 0.047

1.708 ± 0.018

5.166 ± 0.039

6.984 ± 0.04

4.144 ± 0.03

7.703 ± 0.037

1.837 ± 0.021

5.931 ± 0.044

5.297 ± 0.043

9.749 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.673 ± 0.022

3.713 ± 0.03

4.146 ± 0.038

3.664 ± 0.028

5.481 ± 0.049

5.794 ± 0.04

5.101 ± 0.043

6.806 ± 0.037

1.066 ± 0.02

3.756 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski