Inoviridae sp.
Average proteome isoelectric point is 5.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345N1M7|A0A345N1M7_9VIRU Uncharacterized protein OS=Inoviridae sp. OX=2219103 PE=4 SV=1
MM1 pKa = 7.85 KK2 pKa = 10.68 YY3 pKa = 10.52 LILPMALMSAFFVSANEE20 pKa = 3.95 PDD22 pKa = 4.69 LDD24 pKa = 4.3 DD25 pKa = 4.87 LVSPKK30 pKa = 9.98 QVPEE34 pKa = 3.59 IKK36 pKa = 10.6 YY37 pKa = 9.09 FNPNNSLLFNSPKK50 pKa = 10.47 DD51 pKa = 3.54 AFEE54 pKa = 4.28 SRR56 pKa = 11.84 FDD58 pKa = 3.53 EE59 pKa = 4.51 SQFCEE64 pKa = 4.72 GTWSCTIIDD73 pKa = 3.75 EE74 pKa = 4.71 SEE76 pKa = 4.49 TVTGTSVKK84 pKa = 10.36 YY85 pKa = 7.86 YY86 pKa = 10.33 RR87 pKa = 11.84 KK88 pKa = 9.46 YY89 pKa = 9.36 EE90 pKa = 4.15 TYY92 pKa = 10.44 SKK94 pKa = 10.48 CGYY97 pKa = 8.94 PDD99 pKa = 3.42 YY100 pKa = 10.83 YY101 pKa = 10.71 KK102 pKa = 10.85 CNRR105 pKa = 11.84 KK106 pKa = 8.75 EE107 pKa = 3.71 RR108 pKa = 11.84 GFAEE112 pKa = 4.45 VYY114 pKa = 10.36 PIVEE118 pKa = 4.6 EE119 pKa = 4.26 FTYY122 pKa = 10.54 SCPPDD127 pKa = 3.52 NRR129 pKa = 11.84 PLHH132 pKa = 6.36 KK133 pKa = 10.16 IPVPINPVPTDD144 pKa = 3.72 GPKK147 pKa = 10.45 FMCAKK152 pKa = 10.12 PLDD155 pKa = 4.21 NEE157 pKa = 4.17 PDD159 pKa = 3.68 PNAKK163 pKa = 9.77 CDD165 pKa = 3.68 EE166 pKa = 4.53 FGNNSMLPPKK176 pKa = 10.38 AGVGSEE182 pKa = 4.18 GQNACYY188 pKa = 9.48 TNPSNGLSCQYY199 pKa = 10.68 VQGGDD204 pKa = 3.03 NFTATGKK211 pKa = 10.01 QCTGDD216 pKa = 3.53 EE217 pKa = 4.19 NDD219 pKa = 3.6 YY220 pKa = 11.4 GDD222 pKa = 4.6 RR223 pKa = 11.84 PTPEE227 pKa = 4.78 PPPDD231 pKa = 3.84 GADD234 pKa = 3.48 PNCYY238 pKa = 10.2 NYY240 pKa = 10.7 GSQGQVLICDD250 pKa = 3.52 VDD252 pKa = 4.01 PNEE255 pKa = 4.38 GCNPLTINGTTQYY268 pKa = 10.4 QCPSGCGSIDD278 pKa = 3.07 GVYY281 pKa = 10.06 FCSYY285 pKa = 10.64 DD286 pKa = 3.92 DD287 pKa = 4.48 KK288 pKa = 11.66 DD289 pKa = 4.03 GDD291 pKa = 5.38 GIPDD295 pKa = 3.84 DD296 pKa = 4.56 KK297 pKa = 11.42 NGNGVPDD304 pKa = 3.83 KK305 pKa = 11.53 DD306 pKa = 3.77 EE307 pKa = 4.14 TCVNGKK313 pKa = 9.56 CKK315 pKa = 10.39 PNTPDD320 pKa = 3.38 DD321 pKa = 3.99 TTPPTTEE328 pKa = 4.29 KK329 pKa = 10.89 PDD331 pKa = 3.41 MTEE334 pKa = 3.54 TNTRR338 pKa = 11.84 LDD340 pKa = 3.75 GVIGEE345 pKa = 4.73 LDD347 pKa = 3.81 SIGGKK352 pKa = 9.24 INKK355 pKa = 7.96 TNQTLDD361 pKa = 4.31 GINSGIQGIKK371 pKa = 9.82 QGQDD375 pKa = 2.76 KK376 pKa = 10.78 TNGLLTGMSKK386 pKa = 9.22 DD387 pKa = 3.17 TGLIANNTDD396 pKa = 4.02 AIAGNTKK403 pKa = 10.35 GLLEE407 pKa = 4.52 TVSKK411 pKa = 9.99 TDD413 pKa = 3.04 VGNTFNPDD421 pKa = 3.11 LSTGFYY427 pKa = 9.98 EE428 pKa = 4.55 SSYY431 pKa = 10.87 EE432 pKa = 4.08 NGFEE436 pKa = 4.66 GIWAEE441 pKa = 3.91 KK442 pKa = 10.53 SILFEE447 pKa = 3.91 QTEE450 pKa = 4.79 TIQFLQQFRR459 pKa = 11.84 FNAGGSPPDD468 pKa = 3.6 TQLCFNMGGSMDD480 pKa = 4.88 FGCADD485 pKa = 4.83 LPTPSPQLLAILKK498 pKa = 9.92 IFILITAAFLCRR510 pKa = 11.84 ALIFGGG516 pKa = 3.67
Molecular weight: 56.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.925
IPC2_protein 4.19
IPC_protein 4.19
Toseland 3.986
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.012
Grimsley 3.897
Solomon 4.164
Lehninger 4.113
Nozaki 4.266
DTASelect 4.495
Thurlkill 4.012
EMBOSS 4.088
Sillero 4.304
Patrickios 1.291
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.189
Protein with the highest isoelectric point:
>tr|A0A345N1N0|A0A345N1N0_9VIRU DNA replication initiation protein OS=Inoviridae sp. OX=2219103 PE=4 SV=1
MM1 pKa = 7.49 HH2 pKa = 7.05 YY3 pKa = 9.95 KK4 pKa = 10.72 YY5 pKa = 10.97 SDD7 pKa = 3.29 TAQNLIDD14 pKa = 3.72 NRR16 pKa = 11.84 IKK18 pKa = 10.72 RR19 pKa = 11.84 FKK21 pKa = 10.43 KK22 pKa = 9.82 HH23 pKa = 5.69 EE24 pKa = 4.45 ANQTIIDD31 pKa = 3.99 HH32 pKa = 6.84 LSFSFPLSDD41 pKa = 4.43 LRR43 pKa = 11.84 HH44 pKa = 6.17 CKK46 pKa = 9.86 RR47 pKa = 11.84 AGSIGSTVDD56 pKa = 3.12 TQTLFPVVPNIKK68 pKa = 10.28 EE69 pKa = 4.0 EE70 pKa = 4.17 FSTEE74 pKa = 3.62 GLTPDD79 pKa = 3.75 EE80 pKa = 4.25 VLKK83 pKa = 11.15 SLEE86 pKa = 4.19 TQKK89 pKa = 10.58 QRR91 pKa = 11.84 INSRR95 pKa = 11.84 MSDD98 pKa = 3.57 FYY100 pKa = 11.14 INTLRR105 pKa = 11.84 VFSRR109 pKa = 11.84 YY110 pKa = 8.51 VLGFDD115 pKa = 4.99 LSAPRR120 pKa = 11.84 DD121 pKa = 3.77 KK122 pKa = 11.3 GFHH125 pKa = 6.58 GYY127 pKa = 9.25 HH128 pKa = 6.3 NSMNLVTSQGNKK140 pKa = 9.48 VGFVGIGGQRR150 pKa = 11.84 DD151 pKa = 3.36 TVYY154 pKa = 10.61 FQISGEE160 pKa = 4.34 GCKK163 pKa = 10.26 HH164 pKa = 5.88 LWSHH168 pKa = 5.58 TTPFILHH175 pKa = 6.21 HH176 pKa = 6.53 WLSKK180 pKa = 10.47 VLSISNLSRR189 pKa = 11.84 IDD191 pKa = 3.49 IARR194 pKa = 11.84 DD195 pKa = 3.41 CYY197 pKa = 11.5 DD198 pKa = 4.37 DD199 pKa = 5.05 VFNCVNAEE207 pKa = 3.64 KK208 pKa = 10.99 DD209 pKa = 3.64 FFNSAFRR216 pKa = 11.84 RR217 pKa = 11.84 NKK219 pKa = 10.16 GGPSPKK225 pKa = 9.44 MGNHH229 pKa = 6.81 DD230 pKa = 4.64 SISIDD235 pKa = 2.95 RR236 pKa = 11.84 VFDD239 pKa = 3.61 VEE241 pKa = 4.26 MKK243 pKa = 10.79 SFGKK247 pKa = 8.75 RR248 pKa = 11.84 TSPVYY253 pKa = 9.54 WRR255 pKa = 11.84 VYY257 pKa = 9.8 NKK259 pKa = 10.39 KK260 pKa = 10.26 LEE262 pKa = 3.94 QGIDD266 pKa = 3.52 DD267 pKa = 5.68 DD268 pKa = 4.64 SLVWYY273 pKa = 10.28 RR274 pKa = 11.84 NEE276 pKa = 3.87 VEE278 pKa = 4.14 LKK280 pKa = 9.76 KK281 pKa = 10.19 WSVDD285 pKa = 3.44 CLLDD289 pKa = 4.07 PDD291 pKa = 4.16 SAFAGICAFSQQMINTDD308 pKa = 3.58 GVQTSSSSKK317 pKa = 9.2 VSKK320 pKa = 10.81 AGTDD324 pKa = 3.2 LASRR328 pKa = 11.84 VKK330 pKa = 9.4 WVRR333 pKa = 11.84 RR334 pKa = 11.84 MCGKK338 pKa = 10.32 ALADD342 pKa = 3.07 ILEE345 pKa = 4.33 ITEE348 pKa = 4.74 GDD350 pKa = 3.64 MQTVLGLLVPHH361 pKa = 7.13 KK362 pKa = 10.32 YY363 pKa = 8.39 VTGKK367 pKa = 10.51 SLDD370 pKa = 3.26 VPNIYY375 pKa = 10.52 KK376 pKa = 10.5 HH377 pKa = 6.45 ILTEE381 pKa = 3.93 QLRR384 pKa = 11.84 SHH386 pKa = 7.02
Molecular weight: 43.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.667
IPC2_protein 7.936
IPC_protein 7.819
Toseland 7.761
ProMoST 8.346
Dawson 8.595
Bjellqvist 8.799
Wikipedia 8.551
Rodwell 8.639
Grimsley 7.761
Solomon 8.697
Lehninger 8.697
Nozaki 8.99
DTASelect 8.58
Thurlkill 8.668
EMBOSS 8.785
Sillero 8.946
Patrickios 4.596
IPC_peptide 8.697
IPC2_peptide 7.614
IPC2.peptide.svr19 7.758
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1511
105
516
302.2
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.427 ± 1.273
2.184 ± 0.806
7.346 ± 0.549
5.361 ± 0.632
5.493 ± 0.397
7.28 ± 1.173
2.449 ± 0.984
6.353 ± 0.907
7.015 ± 0.847
7.081 ± 0.92
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.052 ± 0.41
5.493 ± 0.894
5.162 ± 1.355
4.236 ± 0.686
3.309 ± 0.811
6.949 ± 0.935
5.824 ± 0.937
5.956 ± 0.962
1.059 ± 0.397
3.971 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here