Capybara microvirus Cap1_SP_48
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4V5|A0A4P8W4V5_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_48 OX=2584779 PE=4 SV=1
MM1 pKa = 7.02 NRR3 pKa = 11.84 KK4 pKa = 9.25 SFIEE8 pKa = 3.94 NSVSKK13 pKa = 9.87 YY14 pKa = 6.94 YY15 pKa = 10.79 HH16 pKa = 6.66 SIEE19 pKa = 4.41 TPPPTNDD26 pKa = 3.39 VVCHH30 pKa = 5.36 VVDD33 pKa = 3.81 VTDD36 pKa = 5.46 DD37 pKa = 3.62 EE38 pKa = 5.16 GNSYY42 pKa = 10.35 PVVAIAEE49 pKa = 4.19 KK50 pKa = 10.29 KK51 pKa = 9.31 YY52 pKa = 11.1 GSFHH56 pKa = 7.72 VSDD59 pKa = 4.82 FDD61 pKa = 4.08 LTNLINAGIDD71 pKa = 3.46 PRR73 pKa = 11.84 SVSISQSSAQYY84 pKa = 10.92 LDD86 pKa = 5.18 DD87 pKa = 4.65 IEE89 pKa = 6.16 HH90 pKa = 6.92 FDD92 pKa = 4.14 SLAKK96 pKa = 10.1 TILSRR101 pKa = 11.84 LDD103 pKa = 3.44 NLEE106 pKa = 4.15 VSQSPSGEE114 pKa = 4.17 TLSIPSPVEE123 pKa = 3.38 ATAPSQNN130 pKa = 3.16
Molecular weight: 14.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.359
IPC2_protein 4.418
IPC_protein 4.342
Toseland 4.164
ProMoST 4.482
Dawson 4.317
Bjellqvist 4.457
Wikipedia 4.228
Rodwell 4.177
Grimsley 4.075
Solomon 4.304
Lehninger 4.266
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.457
Patrickios 3.452
IPC_peptide 4.304
IPC2_peptide 4.444
IPC2.peptide.svr19 4.383
Protein with the highest isoelectric point:
>tr|A0A4P8W7G2|A0A4P8W7G2_9VIRU Peptidase M15 OS=Capybara microvirus Cap1_SP_48 OX=2584779 PE=4 SV=1
MM1 pKa = 7.2 RR2 pKa = 11.84 TCFSILNRR10 pKa = 11.84 SNYY13 pKa = 9.74 FDD15 pKa = 3.45 IEE17 pKa = 4.09 RR18 pKa = 11.84 SKK20 pKa = 11.13 SHH22 pKa = 7.38 FDD24 pKa = 3.18 IEE26 pKa = 5.0 SNALFLNQLNKK37 pKa = 10.29 CNSYY41 pKa = 10.39 EE42 pKa = 3.9 IRR44 pKa = 11.84 NHH46 pKa = 6.59 FEE48 pKa = 3.46 FEE50 pKa = 4.33 EE51 pKa = 4.16 IKK53 pKa = 10.93 KK54 pKa = 10.79 LGGQAFFYY62 pKa = 9.95 TLSYY66 pKa = 11.33 NDD68 pKa = 2.92 AHH70 pKa = 6.84 LPHH73 pKa = 6.53 FHH75 pKa = 7.61 GIPCHH80 pKa = 5.4 DD81 pKa = 4.51 HH82 pKa = 6.41 EE83 pKa = 5.83 DD84 pKa = 4.17 LRR86 pKa = 11.84 ALLNGAFSKK95 pKa = 10.49 RR96 pKa = 11.84 VKK98 pKa = 10.21 RR99 pKa = 11.84 YY100 pKa = 8.58 CNCSLRR106 pKa = 11.84 YY107 pKa = 9.49 FITLEE112 pKa = 4.03 LGEE115 pKa = 4.68 GKK117 pKa = 10.5 GSRR120 pKa = 11.84 GLFKK124 pKa = 10.71 NPHH127 pKa = 4.61 YY128 pKa = 10.96 HH129 pKa = 6.7 CIFYY133 pKa = 10.61 LRR135 pKa = 11.84 PKK137 pKa = 10.23 EE138 pKa = 3.83 GEE140 pKa = 4.04 IYY142 pKa = 11.09 NKK144 pKa = 9.68 IDD146 pKa = 3.75 PLTFRR151 pKa = 11.84 SFIRR155 pKa = 11.84 EE156 pKa = 3.61 YY157 pKa = 9.46 WQGVDD162 pKa = 3.84 IDD164 pKa = 4.66 FSHH167 pKa = 7.44 PSARR171 pKa = 11.84 AKK173 pKa = 10.52 DD174 pKa = 3.86 FRR176 pKa = 11.84 FGICQEE182 pKa = 3.66 GKK184 pKa = 10.09 YY185 pKa = 10.19 FGEE188 pKa = 4.27 ILTSAACRR196 pKa = 11.84 YY197 pKa = 7.96 CCKK200 pKa = 10.03 YY201 pKa = 9.0 TIKK204 pKa = 10.78 SNAVIQHH211 pKa = 5.52 YY212 pKa = 9.2 LSCVSEE218 pKa = 4.08 IQRR221 pKa = 11.84 DD222 pKa = 3.69 FDD224 pKa = 3.83 NSLGGIKK231 pKa = 10.49 YY232 pKa = 9.81 SVSPEE237 pKa = 4.07 EE238 pKa = 4.13 YY239 pKa = 10.43 NSFIDD244 pKa = 3.85 DD245 pKa = 3.39 QVKK248 pKa = 10.06 IFKK251 pKa = 9.95 NRR253 pKa = 11.84 YY254 pKa = 6.32 FVKK257 pKa = 10.17 VRR259 pKa = 11.84 CSQGLGSYY267 pKa = 10.42 GLSQVNDD274 pKa = 3.85 DD275 pKa = 4.4 KK276 pKa = 11.94 VVVPYY281 pKa = 10.82 KK282 pKa = 10.18 STFKK286 pKa = 10.65 SYY288 pKa = 10.17 PIPLYY293 pKa = 10.28 YY294 pKa = 9.98 YY295 pKa = 9.66 RR296 pKa = 11.84 KK297 pKa = 10.19 LYY299 pKa = 10.0 TNHH302 pKa = 6.69 CKK304 pKa = 9.99 NALGNVHH311 pKa = 6.62 YY312 pKa = 9.53 YY313 pKa = 10.66 HH314 pKa = 6.81 NSKK317 pKa = 10.43 GLEE320 pKa = 3.76 RR321 pKa = 11.84 LRR323 pKa = 11.84 NTLHH327 pKa = 6.92 LRR329 pKa = 11.84 IDD331 pKa = 3.71 KK332 pKa = 10.81 AKK334 pKa = 10.66 SDD336 pKa = 3.73 ALKK339 pKa = 10.37 LVSMLSDD346 pKa = 3.2 KK347 pKa = 11.14 DD348 pKa = 3.89 FNLSFTRR355 pKa = 11.84 WLEE358 pKa = 3.99 SNGIILSSSILDD370 pKa = 4.12 LGLCAQRR377 pKa = 11.84 YY378 pKa = 9.08 AEE380 pKa = 4.11 FSVVYY385 pKa = 10.15 KK386 pKa = 10.59 NRR388 pKa = 11.84 YY389 pKa = 6.9 YY390 pKa = 10.89 TDD392 pKa = 4.25 LNCPIDD398 pKa = 3.6 IDD400 pKa = 4.21 LDD402 pKa = 3.75 HH403 pKa = 7.35 EE404 pKa = 4.5 FFIIPVEE411 pKa = 4.26 TEE413 pKa = 3.74 NALHH417 pKa = 6.86 RR418 pKa = 11.84 LAVQKK423 pKa = 10.63 GRR425 pKa = 11.84 YY426 pKa = 7.05 EE427 pKa = 3.87 VHH429 pKa = 6.74 SYY431 pKa = 11.44 ANHH434 pKa = 7.18 PYY436 pKa = 9.39 FSPYY440 pKa = 9.84 YY441 pKa = 10.47 VQFCLLDD448 pKa = 3.62 LLKK451 pKa = 10.91 EE452 pKa = 4.42 FFRR455 pKa = 11.84 QTISTTMYY463 pKa = 9.91 RR464 pKa = 11.84 KK465 pKa = 9.67 KK466 pKa = 10.55 CAIEE470 pKa = 3.63 EE471 pKa = 4.18 TRR473 pKa = 11.84 RR474 pKa = 11.84 VHH476 pKa = 6.82 LSRR479 pKa = 11.84 RR480 pKa = 11.84 FPSS483 pKa = 3.19
Molecular weight: 56.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.035
IPC2_protein 8.112
IPC_protein 7.907
Toseland 7.629
ProMoST 8.463
Dawson 8.639
Bjellqvist 8.916
Wikipedia 8.609
Rodwell 8.682
Grimsley 7.673
Solomon 8.697
Lehninger 8.682
Nozaki 9.019
DTASelect 8.668
Thurlkill 8.712
EMBOSS 8.814
Sillero 9.004
Patrickios 3.757
IPC_peptide 8.682
IPC2_peptide 7.907
IPC2.peptide.svr19 8.076
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1773
130
624
354.6
40.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.979 ± 1.378
1.805 ± 0.53
5.697 ± 0.561
5.133 ± 0.566
5.979 ± 0.711
6.091 ± 0.791
2.538 ± 0.683
5.64 ± 0.546
5.753 ± 0.444
8.404 ± 0.474
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.715
6.43 ± 0.842
3.553 ± 0.478
3.892 ± 0.932
4.625 ± 0.681
9.081 ± 0.779
5.302 ± 0.746
6.035 ± 0.617
0.79 ± 0.283
5.133 ± 1.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here