Pseudonocardia broussonetiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M6JBY6|A0A6M6JBY6_9PSEU Glycosyl transferase OS=Pseudonocardia broussonetiae OX=2736640 GN=HOP40_00250 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84ISVDD6 pKa = 3.28SSLCEE11 pKa = 3.81AHH13 pKa = 5.79SQCYY17 pKa = 8.84VVDD20 pKa = 4.37PDD22 pKa = 5.82LFPLDD27 pKa = 4.2DD28 pKa = 5.31DD29 pKa = 5.26GYY31 pKa = 10.8SAVGQDD37 pKa = 3.1RR38 pKa = 11.84PVPAGEE44 pKa = 3.83EE45 pKa = 3.74DD46 pKa = 3.5AARR49 pKa = 11.84LGVSACPVAALRR61 pKa = 11.84ADD63 pKa = 3.9DD64 pKa = 4.78GGTT67 pKa = 3.18

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M6JJ75|A0A6M6JJ75_9PSEU Ketol-acid reductoisomerase (NADP(+)) OS=Pseudonocardia broussonetiae OX=2736640 GN=ilvC PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6850

0

6850

2156496

29

4812

314.8

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.602 ± 0.05

0.751 ± 0.008

6.381 ± 0.029

5.364 ± 0.028

2.621 ± 0.019

9.791 ± 0.028

2.259 ± 0.014

2.858 ± 0.019

1.235 ± 0.018

10.606 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.6 ± 0.009

1.371 ± 0.014

6.34 ± 0.027

2.377 ± 0.016

8.392 ± 0.029

4.582 ± 0.02

5.813 ± 0.022

9.894 ± 0.03

1.438 ± 0.011

1.725 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski