Amino acid dipepetide frequency for Roseburia sp. CAG:50

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.736AlaAla: 7.736 ± 0.126	1.203AlaCys: 1.203 ± 0.043	5.143AlaAsp: 5.143 ± 0.093	5.802AlaGlu: 5.802 ± 0.087	3.003AlaPhe: 3.003 ± 0.064	6.297AlaGly: 6.297 ± 0.112	1.322AlaHis: 1.322 ± 0.047	5.265AlaIle: 5.265 ± 0.096	4.888AlaLys: 4.888 ± 0.091	6.769AlaLeu: 6.769 ± 0.109	2.639AlaMet: 2.639 ± 0.061	2.791AlaAsn: 2.791 ± 0.068	2.079AlaPro: 2.079 ± 0.052	2.877AlaGln: 2.877 ± 0.071	2.75AlaArg: 2.75 ± 0.066	3.914AlaSer: 3.914 ± 0.083	3.47AlaThr: 3.47 ± 0.072	6.471AlaVal: 6.471 ± 0.115	0.711AlaTrp: 0.711 ± 0.034	3.023AlaTyr: 3.023 ± 0.06	0.004AlaXaa: 0.004 ± 0.002
Cys	1.135CysAla: 1.135 ± 0.045	0.333CysCys: 0.333 ± 0.022	0.929CysAsp: 0.929 ± 0.037	1.054CysGlu: 1.054 ± 0.038	0.627CysPhe: 0.627 ± 0.031	1.654CysGly: 1.654 ± 0.05	0.348CysHis: 0.348 ± 0.02	1.275CysIle: 1.275 ± 0.039	0.838CysLys: 0.838 ± 0.03	1.254CysLeu: 1.254 ± 0.042	0.534CysMet: 0.534 ± 0.028	0.647CysAsn: 0.647 ± 0.029	0.674CysPro: 0.674 ± 0.033	0.519CysGln: 0.519 ± 0.027	0.736CysArg: 0.736 ± 0.031	0.896CysSer: 0.896 ± 0.037	0.906CysThr: 0.906 ± 0.036	1.205CysVal: 1.205 ± 0.044	0.126CysTrp: 0.126 ± 0.015	0.642CysTyr: 0.642 ± 0.027	0.001CysXaa: 0.001 ± 0.001
Asp	4.873AspAla: 4.873 ± 0.086	0.883AspCys: 0.883 ± 0.033	2.812AspAsp: 2.812 ± 0.064	4.484AspGlu: 4.484 ± 0.079	2.776AspPhe: 2.776 ± 0.063	4.534AspGly: 4.534 ± 0.094	1.087AspHis: 1.087 ± 0.044	4.35AspIle: 4.35 ± 0.089	3.097AspLys: 3.097 ± 0.065	4.613AspLeu: 4.613 ± 0.085	1.847AspMet: 1.847 ± 0.047	2.074AspAsn: 2.074 ± 0.059	1.801AspPro: 1.801 ± 0.056	1.678AspGln: 1.678 ± 0.056	2.404AspArg: 2.404 ± 0.057	3.019AspSer: 3.019 ± 0.07	3.414AspThr: 3.414 ± 0.081	4.053AspVal: 4.053 ± 0.076	0.564AspTrp: 0.564 ± 0.029	2.773AspTyr: 2.773 ± 0.06	0.001AspXaa: 0.001 ± 0.001
Glu	5.672GluAla: 5.672 ± 0.101	0.913GluCys: 0.913 ± 0.035	4.165GluAsp: 4.165 ± 0.067	6.679GluGlu: 6.679 ± 0.115	2.387GluPhe: 2.387 ± 0.059	4.1GluGly: 4.1 ± 0.077	1.699GluHis: 1.699 ± 0.044	5.507GluIle: 5.507 ± 0.096	6.332GluLys: 6.332 ± 0.105	6.829GluLeu: 6.829 ± 0.102	2.451GluMet: 2.451 ± 0.055	4.054GluAsn: 4.054 ± 0.078	1.892GluPro: 1.892 ± 0.053	3.531GluGln: 3.531 ± 0.088	3.227GluArg: 3.227 ± 0.062	3.137GluSer: 3.137 ± 0.071	3.945GluThr: 3.945 ± 0.073	4.416GluVal: 4.416 ± 0.081	0.615GluTrp: 0.615 ± 0.029	3.08GluTyr: 3.08 ± 0.076	0.002GluXaa: 0.002 ± 0.002
Phe	3.045PheAla: 3.045 ± 0.068	0.823PheCys: 0.823 ± 0.03	2.504PheAsp: 2.504 ± 0.056	2.613PheGlu: 2.613 ± 0.058	1.718PhePhe: 1.718 ± 0.05	3.087PheGly: 3.087 ± 0.067	0.912PheHis: 0.912 ± 0.037	2.489PheIle: 2.489 ± 0.062	1.629PheLys: 1.629 ± 0.05	3.813PheLeu: 3.813 ± 0.082	1.115PheMet: 1.115 ± 0.037	1.374PheAsn: 1.374 ± 0.046	1.404PhePro: 1.404 ± 0.043	1.348PheGln: 1.348 ± 0.043	1.692PheArg: 1.692 ± 0.048	2.836PheSer: 2.836 ± 0.059	2.216PheThr: 2.216 ± 0.055	2.81PheVal: 2.81 ± 0.059	0.422PheTrp: 0.422 ± 0.026	1.674PheTyr: 1.674 ± 0.044	0.001PheXaa: 0.001 ± 0.001
Gly	5.215GlyAla: 5.215 ± 0.095	1.376GlyCys: 1.376 ± 0.048	3.546GlyAsp: 3.546 ± 0.083	4.269GlyGlu: 4.269 ± 0.078	3.07GlyPhe: 3.07 ± 0.066	4.833GlyGly: 4.833 ± 0.091	1.407GlyHis: 1.407 ± 0.05	6.234GlyIle: 6.234 ± 0.099	5.365GlyLys: 5.365 ± 0.086	5.818GlyLeu: 5.818 ± 0.097	2.645GlyMet: 2.645 ± 0.064	3.149GlyAsn: 3.149 ± 0.068	1.298GlyPro: 1.298 ± 0.049	2.306GlyGln: 2.306 ± 0.052	2.871GlyArg: 2.871 ± 0.07	4.075GlySer: 4.075 ± 0.073	4.605GlyThr: 4.605 ± 0.091	5.072GlyVal: 5.072 ± 0.098	0.911GlyTrp: 0.911 ± 0.054	3.33GlyTyr: 3.33 ± 0.07	0.004GlyXaa: 0.004 ± 0.002
His	1.411HisAla: 1.411 ± 0.041	0.373HisCys: 0.373 ± 0.021	0.97HisAsp: 0.97 ± 0.039	1.21HisGlu: 1.21 ± 0.038	0.865HisPhe: 0.865 ± 0.034	1.436HisGly: 1.436 ± 0.038	0.492HisHis: 0.492 ± 0.034	1.477HisIle: 1.477 ± 0.051	0.971HisLys: 0.971 ± 0.032	1.664HisLeu: 1.664 ± 0.052	0.719HisMet: 0.719 ± 0.033	0.763HisAsn: 0.763 ± 0.031	1.043HisPro: 1.043 ± 0.042	0.668HisGln: 0.668 ± 0.029	0.892HisArg: 0.892 ± 0.031	1.018HisSer: 1.018 ± 0.037	1.14HisThr: 1.14 ± 0.034	1.316HisVal: 1.316 ± 0.045	0.187HisTrp: 0.187 ± 0.014	0.844HisTyr: 0.844 ± 0.032	0.001HisXaa: 0.001 ± 0.001
Ile	5.937IleAla: 5.937 ± 0.087	1.56IleCys: 1.56 ± 0.054	3.909IleAsp: 3.909 ± 0.075	4.347IleGlu: 4.347 ± 0.08	2.892IlePhe: 2.892 ± 0.084	5.203IleGly: 5.203 ± 0.1	1.515IleHis: 1.515 ± 0.045	4.869IleIle: 4.869 ± 0.12	3.495IleLys: 3.495 ± 0.066	6.798IleLeu: 6.798 ± 0.116	1.974IleMet: 1.974 ± 0.053	2.717IleAsn: 2.717 ± 0.059	3.173IlePro: 3.173 ± 0.068	2.453IleGln: 2.453 ± 0.061	3.806IleArg: 3.806 ± 0.084	4.72IleSer: 4.72 ± 0.076	4.079IleThr: 4.079 ± 0.077	4.982IleVal: 4.982 ± 0.09	0.704IleTrp: 0.704 ± 0.029	2.739IleTyr: 2.739 ± 0.068	0.001IleXaa: 0.001 ± 0.001
Lys	4.756LysAla: 4.756 ± 0.092	0.777LysCys: 0.777 ± 0.04	3.541LysAsp: 3.541 ± 0.072	5.959LysGlu: 5.959 ± 0.099	1.883LysPhe: 1.883 ± 0.052	3.676LysGly: 3.676 ± 0.069	1.09LysHis: 1.09 ± 0.033	4.512LysIle: 4.512 ± 0.083	5.782LysLys: 5.782 ± 0.098	5.114LysLeu: 5.114 ± 0.084	2.194LysMet: 2.194 ± 0.053	3.571LysAsn: 3.571 ± 0.066	1.794LysPro: 1.794 ± 0.054	2.539LysGln: 2.539 ± 0.06	2.811LysArg: 2.811 ± 0.066	3.108LysSer: 3.108 ± 0.062	3.623LysThr: 3.623 ± 0.074	4.122LysVal: 4.122 ± 0.078	0.52LysTrp: 0.52 ± 0.028	2.695LysTyr: 2.695 ± 0.067	0.0LysXaa: 0.0 ± 0.0
Leu	6.968LeuAla: 6.968 ± 0.098	1.617LeuCys: 1.617 ± 0.05	4.905LeuAsp: 4.905 ± 0.093	6.191LeuGlu: 6.191 ± 0.098	3.573LeuPhe: 3.573 ± 0.095	5.63LeuGly: 5.63 ± 0.092	1.77LeuHis: 1.77 ± 0.048	5.65LeuIle: 5.65 ± 0.097	5.6LeuLys: 5.6 ± 0.089	7.986LeuLeu: 7.986 ± 0.139	2.638LeuMet: 2.638 ± 0.064	3.756LeuAsn: 3.756 ± 0.069	3.448LeuPro: 3.448 ± 0.067	3.516LeuGln: 3.516 ± 0.073	3.501LeuArg: 3.501 ± 0.081	5.633LeuSer: 5.633 ± 0.094	5.165LeuThr: 5.165 ± 0.088	5.514LeuVal: 5.514 ± 0.089	0.719LeuTrp: 0.719 ± 0.029	3.255LeuTyr: 3.255 ± 0.068	0.002LeuXaa: 0.002 ± 0.002
Met	2.451MetAla: 2.451 ± 0.056	0.401MetCys: 0.401 ± 0.024	1.914MetAsp: 1.914 ± 0.05	2.695MetGlu: 2.695 ± 0.064	1.007MetPhe: 1.007 ± 0.04	2.121MetGly: 2.121 ± 0.055	0.575MetHis: 0.575 ± 0.031	2.322MetIle: 2.322 ± 0.058	2.568MetLys: 2.568 ± 0.061	2.821MetLeu: 2.821 ± 0.062	1.033MetMet: 1.033 ± 0.037	1.617MetAsn: 1.617 ± 0.049	1.204MetPro: 1.204 ± 0.044	1.338MetGln: 1.338 ± 0.04	1.354MetArg: 1.354 ± 0.041	1.819MetSer: 1.819 ± 0.048	1.851MetThr: 1.851 ± 0.045	2.024MetVal: 2.024 ± 0.052	0.208MetTrp: 0.208 ± 0.018	0.974MetTyr: 0.974 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.463AsnAla: 3.463 ± 0.082	0.698AsnCys: 0.698 ± 0.033	2.093AsnAsp: 2.093 ± 0.055	2.799AsnGlu: 2.799 ± 0.072	1.57AsnPhe: 1.57 ± 0.048	4.008AsnGly: 4.008 ± 0.12	0.938AsnHis: 0.938 ± 0.032	3.082AsnIle: 3.082 ± 0.06	2.309AsnLys: 2.309 ± 0.056	3.668AsnLeu: 3.668 ± 0.073	1.339AsnMet: 1.339 ± 0.04	1.734AsnAsn: 1.734 ± 0.05	1.981AsnPro: 1.981 ± 0.054	1.482AsnGln: 1.482 ± 0.048	1.992AsnArg: 1.992 ± 0.052	2.105AsnSer: 2.105 ± 0.056	2.394AsnThr: 2.394 ± 0.069	2.964AsnVal: 2.964 ± 0.068	0.487AsnTrp: 0.487 ± 0.029	1.81AsnTyr: 1.81 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.453ProAla: 2.453 ± 0.065	0.493ProCys: 0.493 ± 0.023	2.364ProAsp: 2.364 ± 0.051	3.608ProGlu: 3.608 ± 0.069	1.415ProPhe: 1.415 ± 0.042	2.468ProGly: 2.468 ± 0.054	0.56ProHis: 0.56 ± 0.028	2.068ProIle: 2.068 ± 0.054	1.929ProLys: 1.929 ± 0.05	2.566ProLeu: 2.566 ± 0.058	0.997ProMet: 0.997 ± 0.033	1.277ProAsn: 1.277 ± 0.039	0.683ProPro: 0.683 ± 0.032	1.186ProGln: 1.186 ± 0.04	0.89ProArg: 0.89 ± 0.035	1.655ProSer: 1.655 ± 0.041	1.629ProThr: 1.629 ± 0.048	2.897ProVal: 2.897 ± 0.051	0.304ProTrp: 0.304 ± 0.021	1.481ProTyr: 1.481 ± 0.042	0.004ProXaa: 0.004 ± 0.002
Gln	2.747GlnAla: 2.747 ± 0.067	0.425GlnCys: 0.425 ± 0.021	1.782GlnAsp: 1.782 ± 0.047	3.168GlnGlu: 3.168 ± 0.076	1.265GlnPhe: 1.265 ± 0.035	2.007GlnGly: 2.007 ± 0.051	0.628GlnHis: 0.628 ± 0.033	3.133GlnIle: 3.133 ± 0.066	3.014GlnLys: 3.014 ± 0.069	3.217GlnLeu: 3.217 ± 0.071	1.484GlnMet: 1.484 ± 0.044	1.944GlnAsn: 1.944 ± 0.056	1.059GlnPro: 1.059 ± 0.034	1.752GlnGln: 1.752 ± 0.049	1.432GlnArg: 1.432 ± 0.049	1.705GlnSer: 1.705 ± 0.044	2.073GlnThr: 2.073 ± 0.052	2.422GlnVal: 2.422 ± 0.055	0.323GlnTrp: 0.323 ± 0.021	1.458GlnTyr: 1.458 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.603ArgAla: 2.603 ± 0.057	0.588ArgCys: 0.588 ± 0.029	2.131ArgAsp: 2.131 ± 0.051	3.48ArgGlu: 3.48 ± 0.078	1.805ArgPhe: 1.805 ± 0.055	2.459ArgGly: 2.459 ± 0.055	0.856ArgHis: 0.856 ± 0.029	3.416ArgIle: 3.416 ± 0.061	3.325ArgLys: 3.325 ± 0.069	3.595ArgLeu: 3.595 ± 0.073	1.671ArgMet: 1.671 ± 0.043	1.977ArgAsn: 1.977 ± 0.047	1.29ArgPro: 1.29 ± 0.047	1.884ArgGln: 1.884 ± 0.052	2.094ArgArg: 2.094 ± 0.06	2.063ArgSer: 2.063 ± 0.046	2.224ArgThr: 2.224 ± 0.055	2.688ArgVal: 2.688 ± 0.061	0.349ArgTrp: 0.349 ± 0.02	1.821ArgTyr: 1.821 ± 0.053	0.002ArgXaa: 0.002 ± 0.002
Ser	4.282SerAla: 4.282 ± 0.077	0.914SerCys: 0.914 ± 0.037	3.356SerAsp: 3.356 ± 0.071	3.934SerGlu: 3.934 ± 0.078	2.432SerPhe: 2.432 ± 0.061	4.997SerGly: 4.997 ± 0.092	0.978SerHis: 0.978 ± 0.035	3.748SerIle: 3.748 ± 0.07	2.835SerLys: 2.835 ± 0.059	4.527SerLeu: 4.527 ± 0.08	1.852SerMet: 1.852 ± 0.047	2.125SerAsn: 2.125 ± 0.058	1.54SerPro: 1.54 ± 0.039	1.713SerGln: 1.713 ± 0.051	2.474SerArg: 2.474 ± 0.062	3.225SerSer: 3.225 ± 0.079	2.791SerThr: 2.791 ± 0.065	4.491SerVal: 4.491 ± 0.078	0.553SerTrp: 0.553 ± 0.03	2.449SerTyr: 2.449 ± 0.071	0.004SerXaa: 0.004 ± 0.002
Thr	4.753ThrAla: 4.753 ± 0.097	0.803ThrCys: 0.803 ± 0.036	3.828ThrAsp: 3.828 ± 0.082	3.984ThrGlu: 3.984 ± 0.076	2.171ThrPhe: 2.171 ± 0.056	4.968ThrGly: 4.968 ± 0.095	0.928ThrHis: 0.928 ± 0.034	3.889ThrIle: 3.889 ± 0.074	2.933ThrLys: 2.933 ± 0.065	4.798ThrLeu: 4.798 ± 0.08	1.565ThrMet: 1.565 ± 0.044	2.105ThrAsn: 2.105 ± 0.052	2.262ThrPro: 2.262 ± 0.054	1.73ThrGln: 1.73 ± 0.046	1.961ThrArg: 1.961 ± 0.044	2.994ThrSer: 2.994 ± 0.058	3.022ThrThr: 3.022 ± 0.073	4.61ThrVal: 4.61 ± 0.099	0.486ThrTrp: 0.486 ± 0.025	2.234ThrTyr: 2.234 ± 0.061	0.001ThrXaa: 0.001 ± 0.001
Val	5.313ValAla: 5.313 ± 0.099	1.349ValCys: 1.349 ± 0.046	4.018ValAsp: 4.018 ± 0.087	4.615ValGlu: 4.615 ± 0.083	2.85ValPhe: 2.85 ± 0.07	4.232ValGly: 4.232 ± 0.083	1.213ValHis: 1.213 ± 0.039	5.187ValIle: 5.187 ± 0.086	4.188ValLys: 4.188 ± 0.08	6.595ValLeu: 6.595 ± 0.094	2.154ValMet: 2.154 ± 0.049	2.919ValAsn: 2.919 ± 0.058	2.592ValPro: 2.592 ± 0.056	2.358ValGln: 2.358 ± 0.063	3.008ValArg: 3.008 ± 0.052	4.641ValSer: 4.641 ± 0.078	4.681ValThr: 4.681 ± 0.094	5.055ValVal: 5.055 ± 0.084	0.701ValTrp: 0.701 ± 0.029	2.8ValTyr: 2.8 ± 0.066	0.0ValXaa: 0.0 ± 0.0
Trp	0.503TrpAla: 0.503 ± 0.028	0.145TrpCys: 0.145 ± 0.015	0.522TrpAsp: 0.522 ± 0.029	0.647TrpGlu: 0.647 ± 0.029	0.477TrpPhe: 0.477 ± 0.029	0.592TrpGly: 0.592 ± 0.031	0.202TrpHis: 0.202 ± 0.015	0.69TrpIle: 0.69 ± 0.032	0.841TrpLys: 0.841 ± 0.036	0.793TrpLeu: 0.793 ± 0.035	0.364TrpMet: 0.364 ± 0.019	0.512TrpAsn: 0.512 ± 0.026	0.167TrpPro: 0.167 ± 0.015	0.436TrpGln: 0.436 ± 0.026	0.365TrpArg: 0.365 ± 0.024	0.493TrpSer: 0.493 ± 0.025	0.394TrpThr: 0.394 ± 0.029	0.504TrpVal: 0.504 ± 0.026	0.296TrpTrp: 0.296 ± 0.042	0.582TrpTyr: 0.582 ± 0.047	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.843TyrAla: 2.843 ± 0.064	0.657TyrCys: 0.657 ± 0.03	2.706TyrAsp: 2.706 ± 0.067	3.09TyrGlu: 3.09 ± 0.061	1.741TyrPhe: 1.741 ± 0.055	3.091TyrGly: 3.091 ± 0.066	0.94TyrHis: 0.94 ± 0.035	2.708TyrIle: 2.708 ± 0.057	2.126TyrLys: 2.126 ± 0.058	3.797TyrLeu: 3.797 ± 0.075	1.079TyrMet: 1.079 ± 0.033	1.809TyrAsn: 1.809 ± 0.054	1.538TyrPro: 1.538 ± 0.049	1.727TyrGln: 1.727 ± 0.048	2.06TyrArg: 2.06 ± 0.057	2.157TyrSer: 2.157 ± 0.062	2.402TyrThr: 2.402 ± 0.07	2.851TyrVal: 2.851 ± 0.07	0.341TyrTrp: 0.341 ± 0.019	1.901TyrTyr: 1.901 ± 0.059	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.004XaaGlu: 0.004 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.005XaaGly: 0.005 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.002XaaLys: 0.002 ± 0.002	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.005XaaSer: 0.005 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.002	0.041XaaXaa: 0.041 ± 0.009

Statistics based on 2482 proteins (807152 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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