Spiroplasma helicoides

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3SKV9|A0A1B3SKV9_9MOLU Uncharacterized protein OS=Spiroplasma helicoides OX=216938 GN=SHELI_v1c06020 PE=4 SV=1
MM1 pKa = 7.5NKK3 pKa = 9.45CQNEE7 pKa = 4.57GCSNDD12 pKa = 4.54INAQDD17 pKa = 3.7PKK19 pKa = 10.61RR20 pKa = 11.84IYY22 pKa = 10.95VYY24 pKa = 10.14DD25 pKa = 3.85EE26 pKa = 4.67KK27 pKa = 10.96IEE29 pKa = 4.41DD30 pKa = 4.2EE31 pKa = 4.38IAVSVCDD38 pKa = 3.38QCYY41 pKa = 10.26KK42 pKa = 10.28KK43 pKa = 11.03SKK45 pKa = 10.69DD46 pKa = 3.55EE47 pKa = 4.36EE48 pKa = 4.16NDD50 pKa = 3.19IDD52 pKa = 3.71WSHH55 pKa = 7.12SVDD58 pKa = 3.82DD59 pKa = 4.26QEE61 pKa = 4.57EE62 pKa = 3.96

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3SJ68|A0A1B3SJ68_9MOLU Uncharacterized protein OS=Spiroplasma helicoides OX=216938 GN=SHELI_v1c00160 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31NGRR28 pKa = 11.84KK29 pKa = 9.01IIKK32 pKa = 9.57ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1144

0

1144

395712

29

1519

345.9

39.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.057 ± 0.075

0.75 ± 0.02

5.853 ± 0.056

6.699 ± 0.078

5.423 ± 0.073

5.005 ± 0.071

1.211 ± 0.024

9.469 ± 0.08

10.237 ± 0.089

9.134 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.131 ± 0.026

7.439 ± 0.076

2.413 ± 0.036

3.023 ± 0.042

2.735 ± 0.043

6.942 ± 0.063

5.046 ± 0.06

6.015 ± 0.054

0.97 ± 0.024

4.446 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski