Aedes camptorhynchus negev-like virus
Average proteome isoelectric point is 7.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RT39|A0A1Z2RT39_9VIRU RdRp OS=Aedes camptorhynchus negev-like virus OX=2010268 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 5.02 SSNVLQFFFDD12 pKa = 3.72 TVLPGSSLEE21 pKa = 4.1 FRR23 pKa = 11.84 EE24 pKa = 4.03 HH25 pKa = 5.67 DD26 pKa = 3.37 HH27 pKa = 7.47 LRR29 pKa = 11.84 FEE31 pKa = 5.07 YY32 pKa = 7.11 EE33 pKa = 3.99 TPMKK37 pKa = 10.84 AEE39 pKa = 4.63 GIMFEE44 pKa = 4.55 LSVPTFKK51 pKa = 10.91 KK52 pKa = 9.52 YY53 pKa = 10.77 DD54 pKa = 3.55 RR55 pKa = 11.84 YY56 pKa = 10.86 SSNIRR61 pKa = 11.84 TSIQYY66 pKa = 9.27 PLHH69 pKa = 6.54 NSQKK73 pKa = 10.31 LNAKK77 pKa = 10.17 AFLEE81 pKa = 4.26 RR82 pKa = 11.84 NGLVPQCQGEE92 pKa = 3.92 IDD94 pKa = 4.41 NISEE98 pKa = 4.17 ANKK101 pKa = 10.48 LLLSFKK107 pKa = 10.35 KK108 pKa = 10.48 LCSHH112 pKa = 6.9 NDD114 pKa = 3.43 FSSKK118 pKa = 10.41 PILPNYY124 pKa = 10.09 SNLEE128 pKa = 3.84 QWISGQPPSVLKK140 pKa = 10.56 ILEE143 pKa = 4.33 SEE145 pKa = 4.03 EE146 pKa = 4.49 GIYY149 pKa = 9.89 DD150 pKa = 3.73 QRR152 pKa = 11.84 FDD154 pKa = 3.54 VYY156 pKa = 11.51 DD157 pKa = 4.95 FIVKK161 pKa = 7.97 TIPKK165 pKa = 9.12 IDD167 pKa = 3.96 LEE169 pKa = 4.76 IGAEE173 pKa = 4.03 FRR175 pKa = 11.84 YY176 pKa = 9.75 KK177 pKa = 10.57 APQTIAYY184 pKa = 7.76 QRR186 pKa = 11.84 KK187 pKa = 7.84 IFNSVFCPLLKK198 pKa = 10.64 EE199 pKa = 3.98 FMDD202 pKa = 3.67 RR203 pKa = 11.84 VEE205 pKa = 4.83 IVMDD209 pKa = 4.56 KK210 pKa = 11.23 NILLYY215 pKa = 11.32 NNMSPVEE222 pKa = 3.86 FAQYY226 pKa = 7.7 FTNIFPVCRR235 pKa = 11.84 YY236 pKa = 9.59 RR237 pKa = 11.84 SLSNFFEE244 pKa = 5.12 IDD246 pKa = 3.24 FSKK249 pKa = 11.0 YY250 pKa = 10.27 DD251 pKa = 3.37 KK252 pKa = 11.26 SQGLVILLFEE262 pKa = 4.32 VLVMQEE268 pKa = 4.02 FGVPTIYY275 pKa = 10.97 LKK277 pKa = 9.97 MWVLMHH283 pKa = 7.3 RR284 pKa = 11.84 FTQIIDD290 pKa = 3.23 RR291 pKa = 11.84 SNRR294 pKa = 11.84 FSAYY298 pKa = 8.93 VQYY301 pKa = 10.58 QRR303 pKa = 11.84 KK304 pKa = 9.85 SGDD307 pKa = 3.06 AGTWRR312 pKa = 11.84 LNTVVQIAILNHH324 pKa = 4.9 VFRR327 pKa = 11.84 LYY329 pKa = 11.46 EE330 pKa = 3.82 MVKK333 pKa = 10.42 NDD335 pKa = 2.89 SCLACFSGDD344 pKa = 4.18 DD345 pKa = 3.51 SLIFCKK351 pKa = 10.45 EE352 pKa = 3.61 IVNIDD357 pKa = 3.48 QKK359 pKa = 11.76 LLFLQTRR366 pKa = 11.84 YY367 pKa = 10.43 NLEE370 pKa = 4.02 AKK372 pKa = 10.19 LMNFSVPYY380 pKa = 9.43 FCSKK384 pKa = 10.57 FLILVDD390 pKa = 3.87 NKK392 pKa = 10.28 WIFVPDD398 pKa = 3.92 TVKK401 pKa = 11.13 LIAKK405 pKa = 9.79 LGRR408 pKa = 11.84 NDD410 pKa = 4.37 LVDD413 pKa = 4.58 DD414 pKa = 4.15 EE415 pKa = 5.71 HH416 pKa = 8.23 VEE418 pKa = 4.24 CYY420 pKa = 10.28 RR421 pKa = 11.84 ISFEE425 pKa = 5.27 DD426 pKa = 3.08 NLYY429 pKa = 10.81 YY430 pKa = 10.8 YY431 pKa = 10.49 KK432 pKa = 10.75 NCNNWTVISYY442 pKa = 10.08 AINDD446 pKa = 3.73 RR447 pKa = 11.84 YY448 pKa = 9.24 KK449 pKa = 10.68 TIGEE453 pKa = 3.75 HH454 pKa = 6.79 DD455 pKa = 3.18 IVYY458 pKa = 10.05 RR459 pKa = 11.84 ALLSSVYY466 pKa = 9.11 TKK468 pKa = 10.87 SSFRR472 pKa = 11.84 RR473 pKa = 11.84 LYY475 pKa = 10.57 DD476 pKa = 3.22 KK477 pKa = 11.29 GVGFVKK483 pKa = 10.61 GKK485 pKa = 10.47 VSSKK489 pKa = 10.52 PKK491 pKa = 10.42 LDD493 pKa = 3.69 FF494 pKa = 4.72
Molecular weight: 58.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.751
IPC2_protein 7.044
IPC_protein 6.985
Toseland 6.751
ProMoST 7.468
Dawson 7.644
Bjellqvist 7.892
Wikipedia 7.571
Rodwell 7.658
Grimsley 6.81
Solomon 7.702
Lehninger 7.717
Nozaki 8.083
DTASelect 7.79
Thurlkill 7.834
EMBOSS 7.878
Sillero 8.112
Patrickios 4.012
IPC_peptide 7.688
IPC2_peptide 7.132
IPC2.peptide.svr19 7.231
Protein with the highest isoelectric point:
>tr|A0A1Z2RT34|A0A1Z2RT34_9VIRU Replicase OS=Aedes camptorhynchus negev-like virus OX=2010268 PE=4 SV=1
MM1 pKa = 6.73 STKK4 pKa = 10.19 PQVTEE9 pKa = 3.79 TSEE12 pKa = 4.24 VQSGTIIASTQSDD25 pKa = 4.17 VKK27 pKa = 10.91 SSSPQSTTKK36 pKa = 10.51 AVNPFGIKK44 pKa = 9.48 ILSGGSVLDD53 pKa = 3.45 SSFIQSVVDD62 pKa = 4.23 AYY64 pKa = 11.68 CNLFSYY70 pKa = 10.6 HH71 pKa = 7.22 PISLFCMVLSLVYY84 pKa = 10.09 FFHH87 pKa = 7.67 KK88 pKa = 10.12 IIKK91 pKa = 9.49 IDD93 pKa = 3.55 EE94 pKa = 4.38 KK95 pKa = 11.2 QDD97 pKa = 3.17 IFSTFHH103 pKa = 6.59 ANIGKK108 pKa = 8.94 AHH110 pKa = 6.51 NNSSSIFFKK119 pKa = 11.12 GFTTLLAIPASLLVNNKK136 pKa = 7.97 LTAATILAFAGPWFAKK152 pKa = 9.78 PSSRR156 pKa = 11.84 NAIIATILISYY167 pKa = 9.15 SLVVTHH173 pKa = 6.54 EE174 pKa = 4.31 PVSIMILSQAFFLLVEE190 pKa = 4.72 LRR192 pKa = 11.84 NPLHH196 pKa = 6.68 KK197 pKa = 10.9 LLVTLFALIVIIIGHH212 pKa = 5.23 GQFGAVFKK220 pKa = 11.11
Molecular weight: 23.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.768
IPC2_protein 8.58
IPC_protein 8.521
Toseland 9.311
ProMoST 9.165
Dawson 9.545
Bjellqvist 9.282
Wikipedia 9.663
Rodwell 9.926
Grimsley 9.575
Solomon 9.633
Lehninger 9.604
Nozaki 9.472
DTASelect 9.209
Thurlkill 9.414
EMBOSS 9.721
Sillero 9.516
Patrickios 5.194
IPC_peptide 9.633
IPC2_peptide 7.892
IPC2.peptide.svr19 7.836
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3492
184
1899
698.4
79.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.866 ± 0.465
1.919 ± 0.446
4.983 ± 0.523
5.584 ± 0.691
6.93 ± 1.027
4.696 ± 0.516
1.89 ± 0.21
8.104 ± 0.936
7.646 ± 0.599
10.195 ± 1.219
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.518 ± 0.192
6.901 ± 0.63
3.666 ± 0.116
2.291 ± 0.459
4.467 ± 0.69
7.446 ± 0.724
5.928 ± 0.693
7.302 ± 0.341
0.601 ± 0.184
4.066 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here