Aedes camptorhynchus negev-like virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Z2RT39|A0A1Z2RT39_9VIRU RdRp OS=Aedes camptorhynchus negev-like virus OX=2010268 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 5.02SSNVLQFFFDD12 pKa = 3.72TVLPGSSLEE21 pKa = 4.1FRR23 pKa = 11.84EE24 pKa = 4.03HH25 pKa = 5.67DD26 pKa = 3.37HH27 pKa = 7.47LRR29 pKa = 11.84FEE31 pKa = 5.07YY32 pKa = 7.11EE33 pKa = 3.99TPMKK37 pKa = 10.84AEE39 pKa = 4.63GIMFEE44 pKa = 4.55LSVPTFKK51 pKa = 10.91KK52 pKa = 9.52YY53 pKa = 10.77DD54 pKa = 3.55RR55 pKa = 11.84YY56 pKa = 10.86SSNIRR61 pKa = 11.84TSIQYY66 pKa = 9.27PLHH69 pKa = 6.54NSQKK73 pKa = 10.31LNAKK77 pKa = 10.17AFLEE81 pKa = 4.26RR82 pKa = 11.84NGLVPQCQGEE92 pKa = 3.92IDD94 pKa = 4.41NISEE98 pKa = 4.17ANKK101 pKa = 10.48LLLSFKK107 pKa = 10.35KK108 pKa = 10.48LCSHH112 pKa = 6.9NDD114 pKa = 3.43FSSKK118 pKa = 10.41PILPNYY124 pKa = 10.09SNLEE128 pKa = 3.84QWISGQPPSVLKK140 pKa = 10.56ILEE143 pKa = 4.33SEE145 pKa = 4.03EE146 pKa = 4.49GIYY149 pKa = 9.89DD150 pKa = 3.73QRR152 pKa = 11.84FDD154 pKa = 3.54VYY156 pKa = 11.51DD157 pKa = 4.95FIVKK161 pKa = 7.97TIPKK165 pKa = 9.12IDD167 pKa = 3.96LEE169 pKa = 4.76IGAEE173 pKa = 4.03FRR175 pKa = 11.84YY176 pKa = 9.75KK177 pKa = 10.57APQTIAYY184 pKa = 7.76QRR186 pKa = 11.84KK187 pKa = 7.84IFNSVFCPLLKK198 pKa = 10.64EE199 pKa = 3.98FMDD202 pKa = 3.67RR203 pKa = 11.84VEE205 pKa = 4.83IVMDD209 pKa = 4.56KK210 pKa = 11.23NILLYY215 pKa = 11.32NNMSPVEE222 pKa = 3.86FAQYY226 pKa = 7.7FTNIFPVCRR235 pKa = 11.84YY236 pKa = 9.59RR237 pKa = 11.84SLSNFFEE244 pKa = 5.12IDD246 pKa = 3.24FSKK249 pKa = 11.0YY250 pKa = 10.27DD251 pKa = 3.37KK252 pKa = 11.26SQGLVILLFEE262 pKa = 4.32VLVMQEE268 pKa = 4.02FGVPTIYY275 pKa = 10.97LKK277 pKa = 9.97MWVLMHH283 pKa = 7.3RR284 pKa = 11.84FTQIIDD290 pKa = 3.23RR291 pKa = 11.84SNRR294 pKa = 11.84FSAYY298 pKa = 8.93VQYY301 pKa = 10.58QRR303 pKa = 11.84KK304 pKa = 9.85SGDD307 pKa = 3.06AGTWRR312 pKa = 11.84LNTVVQIAILNHH324 pKa = 4.9VFRR327 pKa = 11.84LYY329 pKa = 11.46EE330 pKa = 3.82MVKK333 pKa = 10.42NDD335 pKa = 2.89SCLACFSGDD344 pKa = 4.18DD345 pKa = 3.51SLIFCKK351 pKa = 10.45EE352 pKa = 3.61IVNIDD357 pKa = 3.48QKK359 pKa = 11.76LLFLQTRR366 pKa = 11.84YY367 pKa = 10.43NLEE370 pKa = 4.02AKK372 pKa = 10.19LMNFSVPYY380 pKa = 9.43FCSKK384 pKa = 10.57FLILVDD390 pKa = 3.87NKK392 pKa = 10.28WIFVPDD398 pKa = 3.92TVKK401 pKa = 11.13LIAKK405 pKa = 9.79LGRR408 pKa = 11.84NDD410 pKa = 4.37LVDD413 pKa = 4.58DD414 pKa = 4.15EE415 pKa = 5.71HH416 pKa = 8.23VEE418 pKa = 4.24CYY420 pKa = 10.28RR421 pKa = 11.84ISFEE425 pKa = 5.27DD426 pKa = 3.08NLYY429 pKa = 10.81YY430 pKa = 10.8YY431 pKa = 10.49KK432 pKa = 10.75NCNNWTVISYY442 pKa = 10.08AINDD446 pKa = 3.73RR447 pKa = 11.84YY448 pKa = 9.24KK449 pKa = 10.68TIGEE453 pKa = 3.75HH454 pKa = 6.79DD455 pKa = 3.18IVYY458 pKa = 10.05RR459 pKa = 11.84ALLSSVYY466 pKa = 9.11TKK468 pKa = 10.87SSFRR472 pKa = 11.84RR473 pKa = 11.84LYY475 pKa = 10.57DD476 pKa = 3.22KK477 pKa = 11.29GVGFVKK483 pKa = 10.61GKK485 pKa = 10.47VSSKK489 pKa = 10.52PKK491 pKa = 10.42LDD493 pKa = 3.69FF494 pKa = 4.72

Molecular weight:
58.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Z2RT34|A0A1Z2RT34_9VIRU Replicase OS=Aedes camptorhynchus negev-like virus OX=2010268 PE=4 SV=1
MM1 pKa = 6.73STKK4 pKa = 10.19PQVTEE9 pKa = 3.79TSEE12 pKa = 4.24VQSGTIIASTQSDD25 pKa = 4.17VKK27 pKa = 10.91SSSPQSTTKK36 pKa = 10.51AVNPFGIKK44 pKa = 9.48ILSGGSVLDD53 pKa = 3.45SSFIQSVVDD62 pKa = 4.23AYY64 pKa = 11.68CNLFSYY70 pKa = 10.6HH71 pKa = 7.22PISLFCMVLSLVYY84 pKa = 10.09FFHH87 pKa = 7.67KK88 pKa = 10.12IIKK91 pKa = 9.49IDD93 pKa = 3.55EE94 pKa = 4.38KK95 pKa = 11.2QDD97 pKa = 3.17IFSTFHH103 pKa = 6.59ANIGKK108 pKa = 8.94AHH110 pKa = 6.51NNSSSIFFKK119 pKa = 11.12GFTTLLAIPASLLVNNKK136 pKa = 7.97LTAATILAFAGPWFAKK152 pKa = 9.78PSSRR156 pKa = 11.84NAIIATILISYY167 pKa = 9.15SLVVTHH173 pKa = 6.54EE174 pKa = 4.31PVSIMILSQAFFLLVEE190 pKa = 4.72LRR192 pKa = 11.84NPLHH196 pKa = 6.68KK197 pKa = 10.9LLVTLFALIVIIIGHH212 pKa = 5.23GQFGAVFKK220 pKa = 11.11

Molecular weight:
23.93 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3492

184

1899

698.4

79.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.866 ± 0.465

1.919 ± 0.446

4.983 ± 0.523

5.584 ± 0.691

6.93 ± 1.027

4.696 ± 0.516

1.89 ± 0.21

8.104 ± 0.936

7.646 ± 0.599

10.195 ± 1.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.518 ± 0.192

6.901 ± 0.63

3.666 ± 0.116

2.291 ± 0.459

4.467 ± 0.69

7.446 ± 0.724

5.928 ± 0.693

7.302 ± 0.341

0.601 ± 0.184

4.066 ± 0.463

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski