Hubei tombus-like virus 33

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGR2|A0A1L3KGR2_9VIRU RdRp catalytic domain-containing protein OS=Hubei tombus-like virus 33 OX=1923281 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 7.84EE3 pKa = 4.07TPGNLDD9 pKa = 2.87NRR11 pKa = 11.84ITVARR16 pKa = 11.84AAVIGILKK24 pKa = 8.32TCSHH28 pKa = 7.4DD29 pKa = 4.45LVTHH33 pKa = 6.19NNVATLVDD41 pKa = 4.04YY42 pKa = 11.63VMEE45 pKa = 4.34EE46 pKa = 3.95STNIFAEE53 pKa = 4.27SMEE56 pKa = 4.4NEE58 pKa = 3.82NKK60 pKa = 10.2FYY62 pKa = 11.09LGLHH66 pKa = 5.18NRR68 pKa = 11.84KK69 pKa = 9.33ALILDD74 pKa = 3.88RR75 pKa = 11.84QANSRR80 pKa = 11.84VLVGEE85 pKa = 4.6LGDD88 pKa = 3.89LTRR91 pKa = 11.84KK92 pKa = 10.15SLDD95 pKa = 3.06EE96 pKa = 4.04KK97 pKa = 10.91LRR99 pKa = 11.84HH100 pKa = 5.83FSCAVTNYY108 pKa = 9.22VVANQVRR115 pKa = 11.84LNAIMLSGVYY125 pKa = 8.05TEE127 pKa = 5.3CDD129 pKa = 3.36VSLLGYY135 pKa = 8.41TGYY138 pKa = 11.09VFFGGYY144 pKa = 8.05VSAMAILPHH153 pKa = 4.82VHH155 pKa = 7.25KK156 pKa = 10.14LTLDD160 pKa = 3.5QSDD163 pKa = 4.05LTQEE167 pKa = 4.01DD168 pKa = 4.26LNYY171 pKa = 10.55EE172 pKa = 4.6FVAKK176 pKa = 10.24CVEE179 pKa = 4.06EE180 pKa = 3.96AVKK183 pKa = 11.02NEE185 pKa = 3.68LEE187 pKa = 4.11QRR189 pKa = 11.84TKK191 pKa = 10.65MMSLYY196 pKa = 10.77GFYY199 pKa = 10.2HH200 pKa = 6.95RR201 pKa = 11.84KK202 pKa = 9.76NGSDD206 pKa = 3.77CKK208 pKa = 11.2VEE210 pKa = 4.25TPSLAVHH217 pKa = 7.44DD218 pKa = 4.02IDD220 pKa = 5.46KK221 pKa = 11.28LKK223 pKa = 10.87LLSKK227 pKa = 10.48AIRR230 pKa = 11.84KK231 pKa = 8.58RR232 pKa = 11.84HH233 pKa = 4.91EE234 pKa = 4.37LKK236 pKa = 10.78NVCQVSKK243 pKa = 11.33VSGAKK248 pKa = 9.43IAEE251 pKa = 4.3SMVALTGTTVAPVVATTKK269 pKa = 10.1ISTYY273 pKa = 10.82VPITSSSSTVVSSCDD288 pKa = 4.7DD289 pKa = 2.98IFKK292 pKa = 10.5PYY294 pKa = 9.26IKK296 pKa = 10.91SMGLNVMKK304 pKa = 10.35DD305 pKa = 2.99SSYY308 pKa = 11.12IDD310 pKa = 4.34DD311 pKa = 4.9ADD313 pKa = 4.03SVSDD317 pKa = 3.92DD318 pKa = 3.51STINSQEE325 pKa = 4.17SVEE328 pKa = 4.54LNLTNIIVHH337 pKa = 6.18TDD339 pKa = 3.29YY340 pKa = 11.29KK341 pKa = 10.16PAEE344 pKa = 3.97ILEE347 pKa = 4.66GYY349 pKa = 9.12IHH351 pKa = 6.41VNRR354 pKa = 11.84GKK356 pKa = 9.94IVNRR360 pKa = 11.84GDD362 pKa = 3.55IITPGTTCPVACLSIQGIDD381 pKa = 3.24SVKK384 pKa = 10.23PVNLKK389 pKa = 10.61LLTEE393 pKa = 4.53KK394 pKa = 10.62GVKK397 pKa = 10.05LIEE400 pKa = 5.36KK401 pKa = 10.3SDD403 pKa = 3.55TDD405 pKa = 4.07PNKK408 pKa = 10.4MKK410 pKa = 9.97DD411 pKa = 3.38TFEE414 pKa = 4.2SMHH417 pKa = 7.07IIGPVNVDD425 pKa = 3.54VIPGVFNKK433 pKa = 9.38TKK435 pKa = 9.87HH436 pKa = 5.46NEE438 pKa = 3.79YY439 pKa = 10.1VALLGRR445 pKa = 11.84HH446 pKa = 5.61INITLPEE453 pKa = 4.33HH454 pKa = 5.61NKK456 pKa = 9.55NWFKK460 pKa = 10.33YY461 pKa = 6.69QHH463 pKa = 6.31YY464 pKa = 9.26YY465 pKa = 11.57ANFFKK470 pKa = 10.82KK471 pKa = 10.68FNFDD475 pKa = 4.09DD476 pKa = 3.5IDD478 pKa = 4.71RR479 pKa = 11.84YY480 pKa = 8.68TLSFDD485 pKa = 3.19EE486 pKa = 4.86WVSQQKK492 pKa = 7.72TDD494 pKa = 3.4KK495 pKa = 11.16RR496 pKa = 11.84EE497 pKa = 4.24GYY499 pKa = 9.94LKK501 pKa = 10.55AVNSIAGAKK510 pKa = 9.85FDD512 pKa = 3.93DD513 pKa = 3.64ARR515 pKa = 11.84YY516 pKa = 9.38HH517 pKa = 6.09KK518 pKa = 10.41RR519 pKa = 11.84QFFIKK524 pKa = 10.06SEE526 pKa = 3.91IMPPPAEE533 pKa = 4.45GNLADD538 pKa = 4.15KK539 pKa = 10.61APRR542 pKa = 11.84GIQGFKK548 pKa = 11.18DD549 pKa = 3.46NISNMFLGSFIGGVSKK565 pKa = 10.26MVAKK569 pKa = 10.79AGTSDD574 pKa = 3.52KK575 pKa = 11.71NEE577 pKa = 3.72FSYY580 pKa = 11.14TSGWSSMEE588 pKa = 3.55LGAWFNKK595 pKa = 10.53YY596 pKa = 9.96YY597 pKa = 10.84GQTHH601 pKa = 6.09TKK603 pKa = 10.37SKK605 pKa = 10.34LKK607 pKa = 10.26YY608 pKa = 10.07IIMEE612 pKa = 4.96DD613 pKa = 3.74DD614 pKa = 3.58FSSYY618 pKa = 11.42DD619 pKa = 3.4STQGRR624 pKa = 11.84GAHH627 pKa = 5.29EE628 pKa = 4.67MEE630 pKa = 3.86LQFYY634 pKa = 10.81NDD636 pKa = 4.28VIDD639 pKa = 4.45KK640 pKa = 7.67TTLDD644 pKa = 3.7EE645 pKa = 4.11NLKK648 pKa = 10.82KK649 pKa = 10.51NIRR652 pKa = 11.84LTLVNQGHH660 pKa = 5.73TNGVGRR666 pKa = 11.84AHH668 pKa = 7.45KK669 pKa = 10.51YY670 pKa = 9.86SVPHH674 pKa = 5.5TRR676 pKa = 11.84KK677 pKa = 10.51SGDD680 pKa = 3.27QNTSIGNTVINFYY693 pKa = 9.85VHH695 pKa = 6.69YY696 pKa = 10.41IAIQEE701 pKa = 4.05WNKK704 pKa = 9.83IHH706 pKa = 7.46DD707 pKa = 3.8RR708 pKa = 11.84VNFGRR713 pKa = 11.84VADD716 pKa = 4.43FRR718 pKa = 11.84MLGLGDD724 pKa = 4.61DD725 pKa = 4.08NLMAIAIEE733 pKa = 4.09AEE735 pKa = 4.22YY736 pKa = 10.77KK737 pKa = 10.42EE738 pKa = 4.35EE739 pKa = 4.18FMLFVEE745 pKa = 5.83KK746 pKa = 10.33IILSLGLKK754 pKa = 9.94PKK756 pKa = 9.89LACNKK761 pKa = 9.42FPSYY765 pKa = 10.11CSSYY769 pKa = 10.77FMPVQLQNGLHH780 pKa = 6.33SYY782 pKa = 10.32VLAPSATKK790 pKa = 10.61SLTKK794 pKa = 10.12MGWTLKK800 pKa = 7.84TTSKK804 pKa = 10.51KK805 pKa = 10.61DD806 pKa = 3.61SVEE809 pKa = 3.87SRR811 pKa = 11.84VWGNLHH817 pKa = 6.97GIAAFKK823 pKa = 10.02FLPLTRR829 pKa = 11.84VLYY832 pKa = 10.16KK833 pKa = 10.59YY834 pKa = 11.19YY835 pKa = 10.77EE836 pKa = 4.38GLNVKK841 pKa = 9.4AVKK844 pKa = 10.15NDD846 pKa = 2.62EE847 pKa = 4.25WKK849 pKa = 10.92AHH851 pKa = 5.89DD852 pKa = 3.98TSACKK857 pKa = 10.48YY858 pKa = 10.03SSHH861 pKa = 7.61PDD863 pKa = 3.2LLRR866 pKa = 11.84WFSEE870 pKa = 4.38LYY872 pKa = 10.35EE873 pKa = 4.11ITEE876 pKa = 4.25SEE878 pKa = 4.31VTEE881 pKa = 4.27LEE883 pKa = 4.58SYY885 pKa = 10.72LSSSVTKK892 pKa = 10.59HH893 pKa = 5.47KK894 pKa = 10.72GKK896 pKa = 9.7PFVWSHH902 pKa = 5.45DD903 pKa = 3.76VFRR906 pKa = 11.84KK907 pKa = 8.3MLKK910 pKa = 10.12LAA912 pKa = 4.26

Molecular weight: 102.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGR2|A0A1L3KGR2_9VIRU RdRp catalytic domain-containing protein OS=Hubei tombus-like virus 33 OX=1923281 PE=4 SV=1
MM1 pKa = 7.71SSNQRR6 pKa = 11.84KK7 pKa = 7.21QVKK10 pKa = 9.83NNNNNKK16 pKa = 9.86SKK18 pKa = 10.81NALVTLQKK26 pKa = 10.57QGNAKK31 pKa = 9.98KK32 pKa = 10.33NKK34 pKa = 9.51KK35 pKa = 9.52KK36 pKa = 10.83VNQVTNGVRR45 pKa = 11.84LGYY48 pKa = 10.09QVTRR52 pKa = 11.84NSFLKK57 pKa = 10.77ALVSPFCPEE66 pKa = 3.43AYY68 pKa = 10.05GIRR71 pKa = 11.84VPDD74 pKa = 4.5PFPFPTVTHH83 pKa = 7.07HH84 pKa = 7.03LRR86 pKa = 11.84QTTVLGANATGSGAAIFLPSPVFSLIDD113 pKa = 3.37VGAVNGTGATVQSTPFTRR131 pKa = 11.84FNTVGATIPYY141 pKa = 9.72FLFKK145 pKa = 10.11STTVGALNAIYY156 pKa = 8.56GTYY159 pKa = 9.96RR160 pKa = 11.84VVSWGIKK167 pKa = 9.42ISNLQPEE174 pKa = 4.9LSATGRR180 pKa = 11.84ITIGMVPIGDD190 pKa = 3.95SVPSEE195 pKa = 4.09NEE197 pKa = 3.8LVNTSILASSITPVFGTPVSQLGSAGLLQLPSAQMFAVQNLLHH240 pKa = 7.04GDD242 pKa = 3.91LEE244 pKa = 4.56VSGMYY249 pKa = 9.63TNSSFWQFKK258 pKa = 7.86TTDD261 pKa = 3.51TQGNMYY267 pKa = 9.7TSHH270 pKa = 6.98TSGDD274 pKa = 3.47SATRR278 pKa = 11.84NTATGVIATSGYY290 pKa = 10.12KK291 pKa = 10.35DD292 pKa = 4.03PNRR295 pKa = 11.84MMGGCAIAIYY305 pKa = 10.49FEE307 pKa = 4.65GVPASTVNAFQIEE320 pKa = 4.63TIYY323 pKa = 10.64HH324 pKa = 6.19IEE326 pKa = 4.23GSPQISTSASTTPVPSGAEE345 pKa = 3.74KK346 pKa = 10.75SIVGTTDD353 pKa = 2.94VVDD356 pKa = 3.54QAMGVASKK364 pKa = 10.93LEE366 pKa = 3.97NVFTFIDD373 pKa = 3.43KK374 pKa = 11.05GADD377 pKa = 3.25FLNRR381 pKa = 11.84STAAFDD387 pKa = 3.53QIAALGTAAKK397 pKa = 10.83LLMM400 pKa = 4.97

Molecular weight: 42.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1312

400

912

656.0

72.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.707 ± 1.203

1.067 ± 0.298

5.03 ± 1.065

4.726 ± 1.168

4.421 ± 0.304

6.707 ± 1.072

2.668 ± 0.744

5.716 ± 0.114

7.546 ± 1.467

8.003 ± 0.657

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.139

6.174 ± 0.302

3.582 ± 0.875

3.049 ± 0.893

3.201 ± 0.237

8.537 ± 0.374

7.546 ± 1.55

8.003 ± 0.261

0.915 ± 0.218

3.887 ± 0.728
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski