Pelargonium flower break virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q70WU2|Q70WU2_9TOMB RNA-directed RNA polymerase OS=Pelargonium flower break virus OX=35291 GN=p99 PE=2 SV=1
MM1 pKa = 7.41LRR3 pKa = 11.84FGSQLVVGSTLIGGLTAVGLAGLSVRR29 pKa = 11.84ATIGVVEE36 pKa = 4.53FNLRR40 pKa = 11.84CARR43 pKa = 11.84VVGDD47 pKa = 4.51IIRR50 pKa = 11.84DD51 pKa = 3.69PFNALPSAPLFPGPSVEE68 pKa = 4.28VFAEE72 pKa = 4.45EE73 pKa = 3.97VRR75 pKa = 11.84KK76 pKa = 10.25DD77 pKa = 3.67LEE79 pKa = 4.52PEE81 pKa = 4.23LEE83 pKa = 4.27AKK85 pKa = 10.32DD86 pKa = 3.55YY87 pKa = 11.48LVIHH91 pKa = 6.37EE92 pKa = 4.51EE93 pKa = 4.19KK94 pKa = 10.97EE95 pKa = 3.66MDD97 pKa = 3.55EE98 pKa = 4.17EE99 pKa = 4.74GKK101 pKa = 10.72SKK103 pKa = 10.44VVRR106 pKa = 11.84QRR108 pKa = 11.84STVNRR113 pKa = 11.84HH114 pKa = 3.8KK115 pKa = 10.65KK116 pKa = 9.55GRR118 pKa = 11.84FVHH121 pKa = 6.94RR122 pKa = 11.84LVCDD126 pKa = 4.38GKK128 pKa = 9.88NHH130 pKa = 7.3FGGTPSASRR139 pKa = 11.84ANEE142 pKa = 3.98LAVMKK147 pKa = 10.2YY148 pKa = 10.21LVGKK152 pKa = 9.96CRR154 pKa = 11.84EE155 pKa = 3.81HH156 pKa = 7.69HH157 pKa = 6.76LVVQHH162 pKa = 5.21TRR164 pKa = 11.84EE165 pKa = 4.19VCSLAMAAIFTPDD178 pKa = 2.8HH179 pKa = 6.83HH180 pKa = 6.65EE181 pKa = 3.98VNMVRR186 pKa = 11.84EE187 pKa = 4.52MNSHH191 pKa = 6.13AAYY194 pKa = 9.82RR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84VALADD202 pKa = 3.49ASRR205 pKa = 11.84VHH207 pKa = 6.22SWQQEE212 pKa = 4.15LLRR215 pKa = 11.84SPLSWNAWGRR225 pKa = 11.84AWWIANGLPDD235 pKa = 4.01RR236 pKa = 11.84EE237 pKa = 4.16PVRR240 pKa = 11.84FTKK243 pKa = 9.67XGGLFCLEE251 pKa = 4.08GVEE254 pKa = 4.24TGVRR258 pKa = 11.84RR259 pKa = 11.84GTHH262 pKa = 4.61PQMIEE267 pKa = 3.88VEE269 pKa = 4.5KK270 pKa = 11.05NSPPKK275 pKa = 10.15LRR277 pKa = 11.84KK278 pKa = 9.63LFAQRR283 pKa = 11.84LVSSGVEE290 pKa = 3.86YY291 pKa = 10.27RR292 pKa = 11.84VHH294 pKa = 5.83NHH296 pKa = 5.19SFQNLRR302 pKa = 11.84RR303 pKa = 11.84GLLEE307 pKa = 3.48RR308 pKa = 11.84VFYY311 pKa = 11.26VEE313 pKa = 4.31EE314 pKa = 4.05NKK316 pKa = 10.19QLVSCPCPEE325 pKa = 3.83KK326 pKa = 10.95GAFKK330 pKa = 10.8EE331 pKa = 3.65MGYY334 pKa = 10.13LRR336 pKa = 11.84KK337 pKa = 9.76QFVRR341 pKa = 11.84LTPKK345 pKa = 9.93HH346 pKa = 5.79ARR348 pKa = 11.84ISAKK352 pKa = 10.4EE353 pKa = 3.79FVDD356 pKa = 4.8CYY358 pKa = 10.44HH359 pKa = 6.28GRR361 pKa = 11.84KK362 pKa = 9.32KK363 pKa = 10.55SVYY366 pKa = 8.73EE367 pKa = 3.98FAAMTLSEE375 pKa = 4.62RR376 pKa = 11.84PLDD379 pKa = 4.22RR380 pKa = 11.84KK381 pKa = 10.27DD382 pKa = 4.33ASLKK386 pKa = 9.87TFIKK390 pKa = 10.68AEE392 pKa = 4.3KK393 pKa = 9.57FCKK396 pKa = 9.97ADD398 pKa = 3.44PAPRR402 pKa = 11.84VIQPRR407 pKa = 11.84SPRR410 pKa = 11.84YY411 pKa = 8.45NVEE414 pKa = 3.57LGRR417 pKa = 11.84YY418 pKa = 7.81LKK420 pKa = 10.64KK421 pKa = 10.66FEE423 pKa = 3.92HH424 pKa = 5.6TAYY427 pKa = 8.71RR428 pKa = 11.84TLDD431 pKa = 4.02KK432 pKa = 10.64IWGGKK437 pKa = 6.93TVMKK441 pKa = 10.09GYY443 pKa = 10.55SVDD446 pKa = 4.05EE447 pKa = 4.31IGKK450 pKa = 9.2HH451 pKa = 6.05ISEE454 pKa = 4.65AWDD457 pKa = 3.6SFHH460 pKa = 8.03SPVAVGFDD468 pKa = 3.23MSRR471 pKa = 11.84FDD473 pKa = 3.32QHH475 pKa = 8.03VSVPALQFEE484 pKa = 4.69HH485 pKa = 6.59SCYY488 pKa = 10.09LACFPGDD495 pKa = 3.29QHH497 pKa = 7.83LAEE500 pKa = 4.54LLSWQLKK507 pKa = 8.66NSGVGFASNGMVRR520 pKa = 11.84YY521 pKa = 9.88KK522 pKa = 10.74KK523 pKa = 10.34DD524 pKa = 3.21GCRR527 pKa = 11.84MSGDD531 pKa = 3.48MNTALGNCLLACLITRR547 pKa = 11.84HH548 pKa = 5.61LMKK551 pKa = 10.53DD552 pKa = 3.19INCRR556 pKa = 11.84LVNNGDD562 pKa = 3.73DD563 pKa = 4.12CVLFLDD569 pKa = 5.07KK570 pKa = 10.8KK571 pKa = 10.42DD572 pKa = 3.8LPFVVSNLTTGWRR585 pKa = 11.84RR586 pKa = 11.84FGFKK590 pKa = 10.26CIAEE594 pKa = 4.21EE595 pKa = 3.62PVYY598 pKa = 10.29EE599 pKa = 4.12LEE601 pKa = 4.55HH602 pKa = 7.98VRR604 pKa = 11.84FCQMAPVYY612 pKa = 10.61DD613 pKa = 4.21GSQYY617 pKa = 11.6VMLRR621 pKa = 11.84DD622 pKa = 3.9PFVSMSKK629 pKa = 10.75DD630 pKa = 3.59SFSLTHH636 pKa = 6.69WNTTKK641 pKa = 10.61NAKK644 pKa = 8.59QWMKK648 pKa = 10.86SVGICGEE655 pKa = 4.5RR656 pKa = 11.84ITGGLPVVQEE666 pKa = 4.47YY667 pKa = 9.27YY668 pKa = 10.56RR669 pKa = 11.84KK670 pKa = 8.18YY671 pKa = 10.51QEE673 pKa = 3.6IAGDD677 pKa = 3.78VKK679 pKa = 10.63FSHH682 pKa = 6.43SLEE685 pKa = 4.05ITSSGTYY692 pKa = 10.13RR693 pKa = 11.84LAQNSSRR700 pKa = 11.84AYY702 pKa = 9.95GSVSEE707 pKa = 4.18MARR710 pKa = 11.84FSFFLAFGVIPDD722 pKa = 3.77AQIALEE728 pKa = 3.9KK729 pKa = 10.0HH730 pKa = 5.63IRR732 pKa = 11.84DD733 pKa = 3.58MPVAAEE739 pKa = 4.26FGPDD743 pKa = 3.14EE744 pKa = 4.53SLADD748 pKa = 3.84HH749 pKa = 6.66TIEE752 pKa = 4.11WLLKK756 pKa = 10.01

Molecular weight: 85.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q70WT9|Q70WT9_9TOMB Putative movement protein OS=Pelargonium flower break virus OX=35291 GN=p7 PE=2 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84LTCQWEE8 pKa = 4.2DD9 pKa = 2.98VGTGVNRR16 pKa = 11.84RR17 pKa = 11.84VRR19 pKa = 11.84YY20 pKa = 8.47PSQRR24 pKa = 11.84TLSPNGHH31 pKa = 6.82LMVVMGVLGLLWLRR45 pKa = 11.84PFRR48 pKa = 11.84SIYY51 pKa = 10.21TSTFSMPPLINLQHH65 pKa = 6.61ILNLTLLSLILSSFILAEE83 pKa = 3.94RR84 pKa = 11.84VTHH87 pKa = 5.79NHH89 pKa = 5.77YY90 pKa = 11.52SNDD93 pKa = 3.43NSKK96 pKa = 10.67AQYY99 pKa = 10.29IRR101 pKa = 11.84ISTGQQ106 pKa = 2.97

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2389

68

871

398.2

44.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.074 ± 0.43

1.967 ± 0.324

4.311 ± 0.231

5.735 ± 0.842

4.437 ± 0.45

6.949 ± 0.316

3.265 ± 0.526

3.809 ± 0.263

5.483 ± 0.481

8.707 ± 0.687

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.266

3.809 ± 0.266

4.479 ± 0.195

2.93 ± 0.158

7.283 ± 0.545

8.204 ± 0.996

4.981 ± 1.271

9.376 ± 0.649

1.716 ± 0.058

2.805 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski