Shewanella phage SppYZU01
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0DYH6|A0A1V0DYH6_9CAUD Uncharacterized protein OS=Shewanella phage SppYZU01 OX=1965372 GN=SppYZU01_22 PE=4 SV=1
MM1 pKa = 8.03 AEE3 pKa = 3.61 QCTNEE8 pKa = 4.41 DD9 pKa = 3.51 RR10 pKa = 11.84 FNCKK14 pKa = 9.33 YY15 pKa = 10.09 CSKK18 pKa = 11.09 AGVTCDD24 pKa = 3.67 GAPGDD29 pKa = 4.03 TSGTPPTGPRR39 pKa = 11.84 VCVRR43 pKa = 11.84 EE44 pKa = 4.22 KK45 pKa = 9.15 YY46 pKa = 8.96 TEE48 pKa = 3.91 AAGTYY53 pKa = 9.38 IGEE56 pKa = 4.35 KK57 pKa = 10.06 EE58 pKa = 4.18 LYY60 pKa = 10.32 DD61 pKa = 3.73 YY62 pKa = 11.09 VLKK65 pKa = 10.52 EE66 pKa = 3.88 WEE68 pKa = 4.2 FLYY71 pKa = 11.04 SDD73 pKa = 4.02 GTTEE77 pKa = 4.2 TVTSRR82 pKa = 11.84 EE83 pKa = 3.9 RR84 pKa = 11.84 VEE86 pKa = 3.89
Molecular weight: 9.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.315
IPC2_protein 4.647
IPC_protein 4.495
Toseland 4.342
ProMoST 4.495
Dawson 4.418
Bjellqvist 4.622
Wikipedia 4.266
Rodwell 4.329
Grimsley 4.253
Solomon 4.406
Lehninger 4.368
Nozaki 4.533
DTASelect 4.622
Thurlkill 4.342
EMBOSS 4.279
Sillero 4.584
Patrickios 2.003
IPC_peptide 4.418
IPC2_peptide 4.584
IPC2.peptide.svr19 4.554
Protein with the highest isoelectric point:
>tr|A0A1V0DYK1|A0A1V0DYK1_9CAUD Uncharacterized protein OS=Shewanella phage SppYZU01 OX=1965372 GN=SppYZU01_31 PE=4 SV=1
MM1 pKa = 7.1 TRR3 pKa = 11.84 INVVPVEE10 pKa = 4.04 EE11 pKa = 4.61 LTNKK15 pKa = 10.02 HH16 pKa = 5.88 LFAEE20 pKa = 4.43 YY21 pKa = 10.38 RR22 pKa = 11.84 EE23 pKa = 4.52 LPRR26 pKa = 11.84 AMRR29 pKa = 11.84 RR30 pKa = 11.84 AVLATYY36 pKa = 10.41 KK37 pKa = 10.34 SRR39 pKa = 11.84 RR40 pKa = 11.84 PSDD43 pKa = 3.21 IKK45 pKa = 10.56 IAPAYY50 pKa = 10.56 LLGTGHH56 pKa = 6.87 EE57 pKa = 4.55 LFFIDD62 pKa = 3.75 KK63 pKa = 10.18 CAWLYY68 pKa = 10.99 SRR70 pKa = 11.84 WLQLRR75 pKa = 11.84 NEE77 pKa = 4.23 LVARR81 pKa = 11.84 GYY83 pKa = 11.17 NLGEE87 pKa = 3.87 QFQTIVRR94 pKa = 11.84 SRR96 pKa = 11.84 ARR98 pKa = 11.84 FIRR101 pKa = 11.84 RR102 pKa = 11.84 HH103 pKa = 4.93 GSQFYY108 pKa = 10.9 GSYY111 pKa = 10.53 QPTADD116 pKa = 3.21 AAIINRR122 pKa = 11.84 QRR124 pKa = 11.84 IQEE127 pKa = 4.13 RR128 pKa = 11.84 LTT130 pKa = 3.35
Molecular weight: 15.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.882
IPC_protein 10.833
Toseland 10.526
ProMoST 10.438
Dawson 10.716
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 10.745
Grimsley 10.804
Solomon 10.847
Lehninger 10.789
Nozaki 10.511
DTASelect 10.511
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.643
Patrickios 10.379
IPC_peptide 10.833
IPC2_peptide 9.648
IPC2.peptide.svr19 8.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
13579
59
876
277.1
30.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.415 ± 0.481
1.105 ± 0.155
6.289 ± 0.262
6.267 ± 0.269
3.402 ± 0.151
8.44 ± 0.235
1.458 ± 0.197
5.162 ± 0.218
5.126 ± 0.396
7.615 ± 0.238
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.797 ± 0.191
4.05 ± 0.197
4.986 ± 0.367
3.69 ± 0.214
6.009 ± 0.29
5.354 ± 0.25
6.127 ± 0.301
7.327 ± 0.256
1.407 ± 0.139
2.975 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here