Synechococcus sp. (strain ATCC 27167 / PCC 6312)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9RZF1|K9RZF1_SYNP3 DUF4351 domain-containing protein OS=Synechococcus sp. (strain ATCC 27167 / PCC 6312) OX=195253 GN=Syn6312_3415 PE=4 SV=1
MM1 pKa = 7.28TAFTLNPDD9 pKa = 3.49TQLQLNPDD17 pKa = 3.18ITFGDD22 pKa = 3.36VDD24 pKa = 4.08GEE26 pKa = 4.2IIALNIQTGFFLNLNSSASYY46 pKa = 10.51IFSLLDD52 pKa = 3.03KK53 pKa = 9.53ATPRR57 pKa = 11.84TLGDD61 pKa = 2.9IYY63 pKa = 11.11KK64 pKa = 9.85QVQAVYY70 pKa = 10.31DD71 pKa = 3.92VQDD74 pKa = 3.59DD75 pKa = 4.17VCRR78 pKa = 11.84SEE80 pKa = 4.0VSGFITHH87 pKa = 6.43CLEE90 pKa = 4.57VGLLQQLEE98 pKa = 4.26

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9RZC2|K9RZC2_SYNP3 Uncharacterized protein OS=Synechococcus sp. (strain ATCC 27167 / PCC 6312) OX=195253 GN=Syn6312_3375 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 8.94RR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 7.32TGQQVLKK33 pKa = 10.11ARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.47GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.42

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3541

0

3541

1047018

29

5212

295.7

32.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.87 ± 0.042

0.965 ± 0.015

4.784 ± 0.032

5.629 ± 0.047

3.789 ± 0.024

7.205 ± 0.058

1.99 ± 0.025

6.23 ± 0.034

3.66 ± 0.037

11.676 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.882 ± 0.018

3.674 ± 0.052

5.574 ± 0.035

5.967 ± 0.044

5.269 ± 0.038

5.803 ± 0.032

5.883 ± 0.043

6.651 ± 0.033

1.62 ± 0.022

2.88 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski