Cribrihabitans marinus
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H6XM90|A0A1H6XM90_9RHOB Cytochrome oxidase maturation protein cbb3-type OS=Cribrihabitans marinus OX=1227549 GN=SAMN05444007_10419 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 10.64 SEE4 pKa = 4.16 GKK6 pKa = 8.49 TMKK9 pKa = 10.38 KK10 pKa = 9.71 ILFASTALVATAGVAAADD28 pKa = 3.85 VTFGGYY34 pKa = 10.02 GRR36 pKa = 11.84 FGLRR40 pKa = 11.84 YY41 pKa = 8.82 AEE43 pKa = 4.17 QPAGSTASDD52 pKa = 3.41 TFLEE56 pKa = 4.3 SRR58 pKa = 11.84 FRR60 pKa = 11.84 LNIDD64 pKa = 3.26 GTAEE68 pKa = 3.93 TDD70 pKa = 3.26 GGVQFSARR78 pKa = 11.84 VRR80 pKa = 11.84 MQADD84 pKa = 3.47 DD85 pKa = 4.1 NGALNTAGTAGLNGARR101 pKa = 11.84 FTTQYY106 pKa = 10.43 EE107 pKa = 4.46 GFRR110 pKa = 11.84 VDD112 pKa = 3.14 VGNIAGVIDD121 pKa = 4.41 NLPNYY126 pKa = 10.41 YY127 pKa = 10.42 GFEE130 pKa = 4.69 PGLSAFTGQYY140 pKa = 9.77 TGVDD144 pKa = 3.82 YY145 pKa = 11.45 AFDD148 pKa = 4.71 AYY150 pKa = 11.0 SSTGAGVNGVFAQYY164 pKa = 11.4 AFGDD168 pKa = 4.15 FAVSASFSPEE178 pKa = 3.39 EE179 pKa = 4.02 TGAGEE184 pKa = 4.04 QYY186 pKa = 10.7 GISAQYY192 pKa = 10.79 SGANWAVALGYY203 pKa = 10.01 SEE205 pKa = 5.31 NDD207 pKa = 4.12 PIGAGADD214 pKa = 3.53 SSDD217 pKa = 3.62 MLVLTASATFGAFTGVIFVGDD238 pKa = 4.06 EE239 pKa = 4.13 SSDD242 pKa = 3.89 LDD244 pKa = 3.5 PTGANFDD251 pKa = 3.89 TFYY254 pKa = 11.11 GLSVNYY260 pKa = 9.46 EE261 pKa = 3.65 VGAATDD267 pKa = 3.41 VMFSYY272 pKa = 11.6 GDD274 pKa = 3.64 GSATNDD280 pKa = 3.47 TQSFGLGVVQDD291 pKa = 4.02 LGGGVSLRR299 pKa = 11.84 GGIGQNDD306 pKa = 3.96 TAGVDD311 pKa = 3.58 QMVGDD316 pKa = 5.15 LGVQFNFF323 pKa = 4.07
Molecular weight: 33.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.528
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.884
Patrickios 1.125
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A1H7DZ91|A0A1H7DZ91_9RHOB DUF853 domain-containing protein OS=Cribrihabitans marinus OX=1227549 GN=SAMN05444007_11737 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.55 ILNSRR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4013
0
4013
1261868
29
2122
314.4
34.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.607 ± 0.05
0.904 ± 0.013
6.321 ± 0.034
5.965 ± 0.031
3.648 ± 0.024
8.862 ± 0.037
2.08 ± 0.019
4.889 ± 0.028
2.779 ± 0.032
10.16 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.708 ± 0.019
2.37 ± 0.019
5.286 ± 0.026
3.135 ± 0.019
7.383 ± 0.041
4.89 ± 0.021
5.199 ± 0.023
7.222 ± 0.03
1.433 ± 0.017
2.161 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here