Deinococcus koreensis
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K3UYP5|A0A2K3UYP5_9DEIO Restriction endonuclease subunit R OS=Deinococcus koreensis OX=2054903 GN=CVO96_09995 PE=4 SV=1
MM1 pKa = 6.91 NTTPRR6 pKa = 11.84 VLALMAALAAGTAAAATTNTTAGQIITNQATATFTDD42 pKa = 4.15 PTTLAAATPIVSNTVQTVVLPKK64 pKa = 10.18 PGFDD68 pKa = 2.85 IQYY71 pKa = 10.81 ADD73 pKa = 3.72 GSADD77 pKa = 3.38 NTTATAPAPSFDD89 pKa = 3.64 KK90 pKa = 11.04 TGVLPGTTVTTGYY103 pKa = 10.84 VVVNTGNVNGYY114 pKa = 8.52 VVNLAADD121 pKa = 3.84 STGGNAPQDD130 pKa = 3.41 VKK132 pKa = 11.58 YY133 pKa = 11.1 YY134 pKa = 11.08 LDD136 pKa = 4.05 ANNDD140 pKa = 3.72 GVPDD144 pKa = 3.49 SSTPVTSVTLNADD157 pKa = 3.86 NPNTPADD164 pKa = 3.75 EE165 pKa = 5.11 GIVRR169 pKa = 11.84 ILQVITVASTAGTGAQYY186 pKa = 10.78 SASPAGTAPSGSVSATDD203 pKa = 3.29 TSTGITTAYY212 pKa = 9.22 PYY214 pKa = 11.04 AALTEE219 pKa = 4.36 AQANPASTNGDD230 pKa = 3.41 LQYY233 pKa = 11.15 TRR235 pKa = 11.84 VTVFTPTVTNAPADD249 pKa = 4.15 GDD251 pKa = 4.07 PATAGQQTPTTTVVVPPSASTVIDD275 pKa = 3.89 PNNPTSAPGTPSSPSDD291 pKa = 3.42 PTQPGYY297 pKa = 9.62 TDD299 pKa = 3.81 PPVAGSTGAVAVTVVGNVQTAYY321 pKa = 10.12 PPADD325 pKa = 3.39 TNAAADD331 pKa = 3.56 AVNFKK336 pKa = 9.38 NTVTTPAGSPADD348 pKa = 3.94 TVNLFPVNPALNVGDD363 pKa = 4.23 AGYY366 pKa = 9.83 GAPYY370 pKa = 8.48 GTNNGDD376 pKa = 3.84 GSFTLPDD383 pKa = 3.4 GTVVRR388 pKa = 11.84 FLNADD393 pKa = 3.56 GSAPTLVTSPVDD405 pKa = 3.38 GKK407 pKa = 10.73 AYY409 pKa = 9.64 PVVSVPAGGATVSYY423 pKa = 8.55 ITQVVYY429 pKa = 10.45 PDD431 pKa = 4.22 SNSLTNPTPITVVVGADD448 pKa = 3.05 SGNDD452 pKa = 3.25 YY453 pKa = 11.48 NLIADD458 pKa = 4.39 GTTTDD463 pKa = 3.78 KK464 pKa = 11.16 VLPPALQFGDD474 pKa = 3.93 SNGVQTPPTADD485 pKa = 3.51 ASAQPSEE492 pKa = 4.42 TVTPGAAVSTGTPTPGMTTDD512 pKa = 3.45 SSAVFPMDD520 pKa = 2.76 IANPGEE526 pKa = 4.16 YY527 pKa = 10.27 GDD529 pKa = 4.62 TYY531 pKa = 10.21 TLSGSVLVPLSNGTTATVPVKK552 pKa = 10.78 YY553 pKa = 10.28 VDD555 pKa = 3.31 ASGTEE560 pKa = 4.09 LPKK563 pKa = 10.81 NAAGQYY569 pKa = 6.33 ITPVVDD575 pKa = 4.63 ANAEE579 pKa = 4.0 YY580 pKa = 10.12 RR581 pKa = 11.84 VYY583 pKa = 11.0 AVVDD587 pKa = 3.49 IPSNARR593 pKa = 11.84 LTLPGSPLSVQQTVTSNYY611 pKa = 10.8 SNITLQDD618 pKa = 3.46 TNDD621 pKa = 4.83 LIRR624 pKa = 11.84 VGAIGGITVDD634 pKa = 3.48 KK635 pKa = 9.49 YY636 pKa = 10.47 QAVGAAPSQTAAGKK650 pKa = 8.12 TIKK653 pKa = 9.4 TALPGEE659 pKa = 4.48 TINYY663 pKa = 9.52 AIVARR668 pKa = 11.84 NSYY671 pKa = 10.7 NDD673 pKa = 3.29 AVKK676 pKa = 10.87 NFVLSDD682 pKa = 3.37 VSGGSTNVYY691 pKa = 10.86 AFTTFQGAGVVLSGFGAYY709 pKa = 9.31 PSAQALYY716 pKa = 9.44 RR717 pKa = 11.84 LNGGASFSASVPAAGSVTSGLEE739 pKa = 3.78 VAVDD743 pKa = 3.88 SNGDD747 pKa = 3.63 GVFNSADD754 pKa = 3.63 VFPSGASITLNISAQVKK771 pKa = 9.56
Molecular weight: 76.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.694
IPC_protein 3.757
Toseland 3.503
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.567
Grimsley 3.401
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.215
Thurlkill 3.567
EMBOSS 3.757
Sillero 3.872
Patrickios 0.515
IPC_peptide 3.757
IPC2_peptide 3.846
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A2K3V247|A0A2K3V247_9DEIO Sodium:proton antiporter OS=Deinococcus koreensis OX=2054903 GN=CVO96_03895 PE=4 SV=1
MM1 pKa = 7.13 GRR3 pKa = 11.84 SRR5 pKa = 11.84 RR6 pKa = 11.84 QDD8 pKa = 2.96 EE9 pKa = 4.51 TTVLGSSEE17 pKa = 4.3 VGTDD21 pKa = 3.61 LLALRR26 pKa = 11.84 AQLHH30 pKa = 5.41 RR31 pKa = 11.84 AEE33 pKa = 4.52 KK34 pKa = 8.38 ATRR37 pKa = 11.84 RR38 pKa = 11.84 APTPPPVRR46 pKa = 11.84 PQRR49 pKa = 11.84 PQPVKK54 pKa = 9.28 PQRR57 pKa = 11.84 EE58 pKa = 4.16 AEE60 pKa = 4.14 LAGVLTDD67 pKa = 3.44 EE68 pKa = 4.95 FSLHH72 pKa = 6.29 RR73 pKa = 11.84 AHH75 pKa = 7.05 ARR77 pKa = 11.84 LRR79 pKa = 11.84 AAVYY83 pKa = 10.22 DD84 pKa = 4.2 GAYY87 pKa = 9.68 HH88 pKa = 6.99 LCPHH92 pKa = 7.46 AIGHH96 pKa = 6.1 ARR98 pKa = 11.84 AEE100 pKa = 4.37 GFLEE104 pKa = 4.06 HH105 pKa = 7.81 DD106 pKa = 3.87 ILNVLLSGRR115 pKa = 11.84 VRR117 pKa = 11.84 AVYY120 pKa = 10.17 PDD122 pKa = 3.25 DD123 pKa = 5.17 HH124 pKa = 6.78 RR125 pKa = 11.84 WLVCGQFEE133 pKa = 4.62 ACGVSLPLHH142 pKa = 5.72 VVVQHH147 pKa = 5.85 FRR149 pKa = 11.84 SGVGGGHH156 pKa = 6.63 IDD158 pKa = 2.91 IVTAFVPRR166 pKa = 11.84 HH167 pKa = 4.62 PHH169 pKa = 5.26 HH170 pKa = 6.7 VISRR174 pKa = 11.84 ARR176 pKa = 11.84 LAVMLRR182 pKa = 11.84 YY183 pKa = 9.93 DD184 pKa = 3.82 DD185 pKa = 3.92 EE186 pKa = 4.66 QIRR189 pKa = 11.84 THH191 pKa = 6.06 TAAPTPKK198 pKa = 9.83 AGHH201 pKa = 6.81 RR202 pKa = 11.84 SRR204 pKa = 11.84 GRR206 pKa = 11.84 WKK208 pKa = 10.52 KK209 pKa = 10.77 SSS211 pKa = 3.16
Molecular weight: 23.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.472
IPC_protein 10.467
Toseland 10.511
ProMoST 10.321
Dawson 10.628
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.657
Grimsley 10.687
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.394
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.57
Patrickios 10.35
IPC_peptide 10.774
IPC2_peptide 9.633
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3967
0
3967
1274002
29
3267
321.1
34.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.684 ± 0.053
0.566 ± 0.011
5.012 ± 0.034
5.317 ± 0.037
3.103 ± 0.024
9.508 ± 0.047
2.098 ± 0.024
3.398 ± 0.029
2.191 ± 0.03
11.87 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.832 ± 0.019
2.238 ± 0.024
6.027 ± 0.036
3.817 ± 0.025
7.553 ± 0.037
5.343 ± 0.031
5.941 ± 0.04
7.836 ± 0.029
1.395 ± 0.016
2.271 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here