Helicobacter didelphidarum
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2279 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8I7X6|A0A3D8I7X6_9HELI Uncharacterized protein OS=Helicobacter didelphidarum OX=2040648 GN=CQA53_10405 PE=4 SV=1
MM1 pKa = 7.21 YY2 pKa = 9.38 PLLEE6 pKa = 4.6 SDD8 pKa = 5.22 ILICPHH14 pKa = 6.56 NGIINLKK21 pKa = 9.77 SNNGSLLEE29 pKa = 3.99 IEE31 pKa = 4.46 GAGVITQSDD40 pKa = 4.56 LLNASIVGCTNSIAGFPTPCSCIATIPPNAISSMLEE76 pKa = 3.8 VNNEE80 pKa = 3.8 GVILQSQIHH89 pKa = 6.52 TILTDD94 pKa = 2.94 KK95 pKa = 10.84 GFPIQIQTKK104 pKa = 8.64 SPNNEE109 pKa = 3.66 VYY111 pKa = 10.95 LDD113 pKa = 4.35 DD114 pKa = 4.84 SDD116 pKa = 5.52 NLASDD121 pKa = 3.15 IDD123 pKa = 4.03 EE124 pKa = 4.7 NPNEE128 pKa = 4.0 NRR130 pKa = 11.84 RR131 pKa = 3.64
Molecular weight: 14.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.849
IPC2_protein 4.113
IPC_protein 4.024
Toseland 3.846
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.91
Rodwell 3.859
Grimsley 3.757
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.923
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.032
Protein with the highest isoelectric point:
>tr|A0A3D8ILT1|A0A3D8ILT1_9HELI Uncharacterized protein OS=Helicobacter didelphidarum OX=2040648 GN=CQA53_04580 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.32 RR14 pKa = 11.84 THH16 pKa = 6.21 GFRR19 pKa = 11.84 EE20 pKa = 3.79 RR21 pKa = 11.84 MKK23 pKa = 9.26 TKK25 pKa = 10.15 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.41 GRR39 pKa = 11.84 KK40 pKa = 8.41 RR41 pKa = 11.84 LSVAA45 pKa = 3.33
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.164
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.901
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2279
0
2279
689512
22
2882
302.6
34.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.784 ± 0.05
1.254 ± 0.023
5.25 ± 0.036
6.394 ± 0.064
5.279 ± 0.052
5.436 ± 0.063
2.163 ± 0.027
9.298 ± 0.05
8.12 ± 0.053
9.75 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.391 ± 0.021
6.504 ± 0.063
2.937 ± 0.03
4.184 ± 0.045
3.508 ± 0.029
6.86 ± 0.039
4.966 ± 0.039
4.868 ± 0.05
0.744 ± 0.015
4.31 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here