Streptococcus satellite phage Javan21

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZG89|A0A4D5ZG89_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan21 OX=2558570 GN=JavanS21_0008 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.87MTGSYY38 pKa = 7.51EE39 pKa = 4.41QPVIDD44 pKa = 3.87ATYY47 pKa = 10.82HH48 pKa = 6.24NDD50 pKa = 3.59DD51 pKa = 3.72QGGHH55 pKa = 6.59ANQLVLEE62 pKa = 4.2YY63 pKa = 11.33AEE65 pKa = 4.34DD66 pKa = 3.58RR67 pKa = 11.84KK68 pKa = 10.83LNKK71 pKa = 9.29VFKK74 pKa = 10.18RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.31DD89 pKa = 3.62MTDD92 pKa = 3.27EE93 pKa = 4.37EE94 pKa = 5.52LDD96 pKa = 3.85DD97 pKa = 3.95VAQEE101 pKa = 4.22TEE103 pKa = 3.94LLKK106 pKa = 10.77EE107 pKa = 4.24VEE109 pKa = 4.4STDD112 pKa = 3.21KK113 pKa = 11.08KK114 pKa = 10.57RR115 pKa = 11.84VVPLFDD121 pKa = 3.75NSQEE125 pKa = 4.21KK126 pKa = 10.53ADD128 pKa = 3.78KK129 pKa = 10.61QDD131 pKa = 2.52IFMAFISDD139 pKa = 3.71HH140 pKa = 5.87NQLAEE145 pKa = 4.25HH146 pKa = 6.45VSMNYY151 pKa = 10.65KK152 pKa = 10.63KK153 pKa = 8.08MTQEE157 pKa = 3.76DD158 pKa = 3.9LGAVLEE164 pKa = 4.88SISQGFNYY172 pKa = 9.9LYY174 pKa = 11.24DD175 pKa = 3.55MAIEE179 pKa = 4.68GEE181 pKa = 4.45LLVKK185 pKa = 10.71

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZIC0|A0A4D5ZIC0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan21 OX=2558570 GN=JavanS21_0011 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 10.27INEE5 pKa = 4.22VKK7 pKa = 10.77KK8 pKa = 10.72KK9 pKa = 10.13DD10 pKa = 3.24GTVIYY15 pKa = 9.86RR16 pKa = 11.84ASVYY20 pKa = 10.73LGTDD24 pKa = 2.51KK25 pKa = 10.85VTGKK29 pKa = 10.64KK30 pKa = 7.91VTTKK34 pKa = 9.18ITGRR38 pKa = 11.84TKK40 pKa = 10.6KK41 pKa = 9.66EE42 pKa = 3.58VRR44 pKa = 11.84EE45 pKa = 3.98KK46 pKa = 10.72AKK48 pKa = 10.48QGAVEE53 pKa = 4.81FIKK56 pKa = 11.1NGSTRR61 pKa = 11.84FKK63 pKa = 9.21ATSVTSYY70 pKa = 11.34QEE72 pKa = 4.91LATLWWDD79 pKa = 3.74SYY81 pKa = 11.1KK82 pKa = 9.85HH83 pKa = 4.21TVKK86 pKa = 10.92YY87 pKa = 8.4NTQLATEE94 pKa = 4.37KK95 pKa = 10.61LLTVHH100 pKa = 6.77VIPIFGAYY108 pKa = 9.53KK109 pKa = 10.15LDD111 pKa = 3.67KK112 pKa = 10.12LTTPLIQSIINKK124 pKa = 9.54LADD127 pKa = 3.45KK128 pKa = 9.75TNKK131 pKa = 10.06GEE133 pKa = 4.09RR134 pKa = 11.84KK135 pKa = 9.79AYY137 pKa = 9.6LHH139 pKa = 6.59YY140 pKa = 11.09DD141 pKa = 4.1RR142 pKa = 11.84IHH144 pKa = 6.67ALNKK148 pKa = 10.16RR149 pKa = 11.84ILQYY153 pKa = 10.96GVIMQAIPFNPARR166 pKa = 11.84EE167 pKa = 4.24VILPRR172 pKa = 11.84NTKK175 pKa = 9.3KK176 pKa = 10.99ANTKK180 pKa = 9.27RR181 pKa = 11.84VKK183 pKa = 10.5HH184 pKa = 5.63FEE186 pKa = 3.77NDD188 pKa = 3.02EE189 pKa = 3.81LRR191 pKa = 11.84TFFNYY196 pKa = 10.41LNNLDD201 pKa = 3.4KK202 pKa = 11.07SKK204 pKa = 10.89YY205 pKa = 7.95RR206 pKa = 11.84NFYY209 pKa = 10.5EE210 pKa = 3.92VTLYY214 pKa = 11.03KK215 pKa = 10.48FLLATGCRR223 pKa = 11.84INEE226 pKa = 3.8ALALNWSDD234 pKa = 3.65IDD236 pKa = 3.87LDD238 pKa = 3.8NAVVHH243 pKa = 5.5ITKK246 pKa = 9.24TLNYY250 pKa = 8.48KK251 pKa = 9.4QEE253 pKa = 4.1INSPKK258 pKa = 10.23SKK260 pKa = 10.48SSYY263 pKa = 10.17RR264 pKa = 11.84DD265 pKa = 2.84IDD267 pKa = 3.51IDD269 pKa = 3.84SQTITMLKK277 pKa = 9.08QYY279 pKa = 10.12RR280 pKa = 11.84RR281 pKa = 11.84RR282 pKa = 11.84QIQEE286 pKa = 2.96AWKK289 pKa = 9.76LGRR292 pKa = 11.84SEE294 pKa = 4.29TVVFSDD300 pKa = 6.41FIHH303 pKa = 7.11KK304 pKa = 10.24YY305 pKa = 8.57PNNRR309 pKa = 11.84TLQTRR314 pKa = 11.84LRR316 pKa = 11.84THH318 pKa = 6.95FKK320 pKa = 10.18RR321 pKa = 11.84ANVPNIGFHH330 pKa = 6.08GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.65ASLLLNTGIPYY347 pKa = 10.09KK348 pKa = 10.14EE349 pKa = 3.64LQYY352 pKa = 11.41RR353 pKa = 11.84LGHH356 pKa = 5.67STLSMTMDD364 pKa = 3.58IYY366 pKa = 11.68SHH368 pKa = 6.98LSKK371 pKa = 11.0EE372 pKa = 4.25NAKK375 pKa = 10.34KK376 pKa = 10.33AVSFFEE382 pKa = 4.08TAINSII388 pKa = 4.07

Molecular weight: 45.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

0

21

3540

40

464

168.6

19.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.932 ± 0.431

0.847 ± 0.217

5.96 ± 0.488

7.74 ± 0.491

4.04 ± 0.299

4.633 ± 0.31

1.949 ± 0.292

6.554 ± 0.402

9.633 ± 0.4

8.729 ± 0.587

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.354

5.424 ± 0.339

3.023 ± 0.485

4.633 ± 0.315

5.0 ± 0.407

6.158 ± 0.728

6.667 ± 0.584

5.0 ± 0.453

0.904 ± 0.166

5.085 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski