Rivularia sp. PCC 7116

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Rivulariaceae; Rivularia; unclassified Rivularia (in: Bacteria)

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9R7V8|K9R7V8_9CYAN Uncharacterized protein OS=Rivularia sp. PCC 7116 OX=373994 GN=Riv7116_0961 PE=4 SV=1
MM1 pKa = 7.1QVNFSNSDD9 pKa = 3.61LFSEE13 pKa = 4.68SANINYY19 pKa = 7.32ITPTNEE25 pKa = 3.89LSDD28 pKa = 4.74DD29 pKa = 3.84LTSNSLNFLARR40 pKa = 11.84SSIEE44 pKa = 3.78YY45 pKa = 9.79SHH47 pKa = 6.97NDD49 pKa = 3.4SAILNQNNSDD59 pKa = 3.88LQDD62 pKa = 3.44VGSTNQLSADD72 pKa = 4.1SYY74 pKa = 11.57SSNQTGTNQTANFSALDD91 pKa = 3.46SDD93 pKa = 4.69EE94 pKa = 4.54YY95 pKa = 11.41SSEE98 pKa = 4.0TGYY101 pKa = 11.81GLVDD105 pKa = 3.62ASEE108 pKa = 4.03AVARR112 pKa = 11.84VIGEE116 pKa = 4.12DD117 pKa = 3.47TFDD120 pKa = 4.41DD121 pKa = 4.24VPDD124 pKa = 4.03KK125 pKa = 11.38GGKK128 pKa = 8.26NWGADD133 pKa = 2.89AVNAPEE139 pKa = 4.16VWEE142 pKa = 3.78QGYY145 pKa = 7.91TGEE148 pKa = 4.35GVVVAVLDD156 pKa = 3.59TGVDD160 pKa = 3.7YY161 pKa = 9.97THH163 pKa = 7.89DD164 pKa = 3.92DD165 pKa = 3.69LKK167 pKa = 11.66NNIWTNSGEE176 pKa = 4.05IEE178 pKa = 4.54NNGKK182 pKa = 9.95DD183 pKa = 3.78DD184 pKa = 5.19DD185 pKa = 4.82GNGYY189 pKa = 9.67IDD191 pKa = 6.32DD192 pKa = 4.47FYY194 pKa = 11.72GWNFDD199 pKa = 3.52GDD201 pKa = 4.14NNSTIDD207 pKa = 3.46VDD209 pKa = 3.68GHH211 pKa = 5.11GTHH214 pKa = 6.47VSGTIAGEE222 pKa = 3.99KK223 pKa = 10.25NGFGVTGIAYY233 pKa = 8.1DD234 pKa = 3.84AQIMPVKK241 pKa = 10.67VLDD244 pKa = 4.31DD245 pKa = 4.24FGSGSNTAVADD256 pKa = 4.8GIYY259 pKa = 10.32YY260 pKa = 10.66AVDD263 pKa = 3.07NGADD267 pKa = 3.88VINLSLGGSFPSFGVSEE284 pKa = 5.25AIQYY288 pKa = 10.72ASEE291 pKa = 4.08QGVTVVMAAGNSGGDD306 pKa = 3.42MPLYY310 pKa = 8.33PARR313 pKa = 11.84YY314 pKa = 8.03ADD316 pKa = 3.68EE317 pKa = 4.26YY318 pKa = 10.61GIAVGAIDD326 pKa = 4.45EE327 pKa = 4.74DD328 pKa = 4.57KK329 pKa = 11.75NMASFSNQAGSEE341 pKa = 3.98EE342 pKa = 4.42LTYY345 pKa = 10.23VTAPGVDD352 pKa = 3.24IYY354 pKa = 11.25STLPDD359 pKa = 3.75NKK361 pKa = 9.76YY362 pKa = 10.06EE363 pKa = 4.37SYY365 pKa = 10.86SGTSMATPHH374 pKa = 5.65VAGVVALMLSANPSFDD390 pKa = 3.95SNLIRR395 pKa = 11.84QTLEE399 pKa = 3.73EE400 pKa = 4.05TSKK403 pKa = 11.44NEE405 pKa = 4.41ASNQPILPDD414 pKa = 3.34LTDD417 pKa = 3.88YY418 pKa = 11.11FPDD421 pKa = 5.65LIPSGDD427 pKa = 3.65SEE429 pKa = 5.15FNFDD433 pKa = 4.19FDD435 pKa = 5.72SNPIMPSFSRR445 pKa = 11.84SFDD448 pKa = 3.22SGKK451 pKa = 9.21TMVFDD456 pKa = 4.56SSMSIDD462 pKa = 3.86KK463 pKa = 10.45SAKK466 pKa = 9.48PYY468 pKa = 10.67SEE470 pKa = 4.12SQFINYY476 pKa = 7.33QQNAWKK482 pKa = 10.45NPNNRR487 pKa = 11.84LFSDD491 pKa = 5.01DD492 pKa = 3.73IEE494 pKa = 5.48SMLEE498 pKa = 3.65EE499 pKa = 5.37DD500 pKa = 4.02EE501 pKa = 4.37VLLRR505 pKa = 11.84LEE507 pKa = 4.2RR508 pKa = 5.34

Molecular weight: 54.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9R5Q9|K9R5Q9_9CYAN Uncharacterized protein OS=Rivularia sp. PCC 7116 OX=373994 GN=Riv7116_0077 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.93RR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.01KK37 pKa = 9.6GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6617

0

6617

2262525

29

6379

341.9

38.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.54 ± 0.033

0.968 ± 0.011

5.013 ± 0.031

6.543 ± 0.035

4.193 ± 0.026

6.546 ± 0.037

1.601 ± 0.017

7.251 ± 0.028

5.836 ± 0.035

10.469 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.785 ± 0.015

5.348 ± 0.037

4.212 ± 0.023

4.897 ± 0.028

4.661 ± 0.026

6.887 ± 0.026

5.465 ± 0.033

6.376 ± 0.023

1.315 ± 0.014

3.094 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski