Chelonobacter oris
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2284 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A3B8I1|A0A0A3B8I1_9PAST Uncharacterized protein OS=Chelonobacter oris OX=505317 GN=OA57_09770 PE=4 SV=1
MM1 pKa = 7.65 AIVGLFYY8 pKa = 11.01 GSDD11 pKa = 3.2 TGNTEE16 pKa = 4.47 NIAKK20 pKa = 9.27 MIQKK24 pKa = 10.41 KK25 pKa = 10.63 LGDD28 pKa = 3.9 NVVDD32 pKa = 3.46 IRR34 pKa = 11.84 DD35 pKa = 3.38 IAKK38 pKa = 10.28 SSKK41 pKa = 10.26 EE42 pKa = 4.2 DD43 pKa = 2.92 ISAYY47 pKa = 10.64 DD48 pKa = 4.34 FILIGIPTWYY58 pKa = 10.23 YY59 pKa = 11.56 GEE61 pKa = 4.97 AQADD65 pKa = 3.42 WDD67 pKa = 4.41 DD68 pKa = 4.28 FFPTLEE74 pKa = 4.91 EE75 pKa = 4.09 IDD77 pKa = 3.96 FTDD80 pKa = 4.3 KK81 pKa = 10.68 IVGIFGCGDD90 pKa = 3.21 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.34 AMGTVRR106 pKa = 11.84 NIIEE110 pKa = 4.05 PHH112 pKa = 5.94 GATIVGHH119 pKa = 6.62 WPTEE123 pKa = 4.28 GYY125 pKa = 10.5 SFEE128 pKa = 4.48 ASQALVDD135 pKa = 3.95 GNTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.9 RR148 pKa = 11.84 QPEE151 pKa = 4.27 LTEE154 pKa = 3.79 QRR156 pKa = 11.84 VSAWVKK162 pKa = 9.65 QIYY165 pKa = 10.52 DD166 pKa = 3.59 EE167 pKa = 4.62 MYY169 pKa = 10.46 LAEE172 pKa = 4.58 LVV174 pKa = 3.53
Molecular weight: 19.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.821
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|A0A0A3ANE3|A0A0A3ANE3_9PAST Transcriptional regulator OS=Chelonobacter oris OX=505317 GN=OA57_03595 PE=4 SV=1
MM1 pKa = 7.74 RR2 pKa = 11.84 GNWLIQSLMVACTFLVALVMEE23 pKa = 4.48 ITPWSSEE30 pKa = 3.81 FQGFRR35 pKa = 11.84 PSWLVLVLLYY45 pKa = 9.88 WVLAIPNKK53 pKa = 10.12 ISIGWAFVVGLIWDD67 pKa = 4.59 LSLGSILGVHH77 pKa = 6.72 ALVLSLFAYY86 pKa = 10.33 LIASNHH92 pKa = 4.95 LVLRR96 pKa = 11.84 NLSLWLQSILVIVSVVTIHH115 pKa = 7.37 IIIFLIEE122 pKa = 4.44 LFMHH126 pKa = 6.19 SARR129 pKa = 11.84 FNSQEE134 pKa = 4.2 LIGAVISGVLWPWVFLLLRR153 pKa = 11.84 TLRR156 pKa = 11.84 RR157 pKa = 11.84 KK158 pKa = 9.45 FHH160 pKa = 6.25 LRR162 pKa = 3.21
Molecular weight: 18.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.619
IPC_protein 10.57
Toseland 10.467
ProMoST 10.847
Dawson 10.628
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.701
Grimsley 10.701
Solomon 10.76
Lehninger 10.716
Nozaki 10.482
DTASelect 10.379
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.54
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2284
0
2284
744759
32
2505
326.1
36.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.223 ± 0.061
1.046 ± 0.02
5.118 ± 0.04
5.626 ± 0.049
4.243 ± 0.041
7.095 ± 0.056
2.173 ± 0.022
6.809 ± 0.05
5.164 ± 0.039
10.91 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.026
4.423 ± 0.043
3.908 ± 0.032
5.09 ± 0.054
4.863 ± 0.041
5.847 ± 0.042
5.016 ± 0.039
6.66 ± 0.042
1.201 ± 0.019
3.177 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here