Saccharibacillus sacchari DSM 19268

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Saccharibacillus; Saccharibacillus sacchari

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011AM60|A0A011AM60_9BACL Putative membrane protein OS=Saccharibacillus sacchari DSM 19268 OX=915437 GN=SacsacDRAFT_0026 PE=4 SV=1
MM1 pKa = 7.41SLNVTQQAADD11 pKa = 3.45WYY13 pKa = 9.84KK14 pKa = 11.02KK15 pKa = 10.42EE16 pKa = 5.31LGLQSGDD23 pKa = 3.44YY24 pKa = 9.58IRR26 pKa = 11.84FYY28 pKa = 11.47ARR30 pKa = 11.84YY31 pKa = 9.36SNTSDD36 pKa = 2.8IHH38 pKa = 6.68PGFSLGIATEE48 pKa = 4.45MPSSPGLTQSSEE60 pKa = 4.08GITFYY65 pKa = 10.62MEE67 pKa = 5.57EE68 pKa = 4.19KK69 pKa = 10.67DD70 pKa = 3.72LWYY73 pKa = 10.81LDD75 pKa = 3.85GYY77 pKa = 9.72RR78 pKa = 11.84LNVTYY83 pKa = 10.47LPEE86 pKa = 6.07HH87 pKa = 7.37DD88 pKa = 5.99DD89 pKa = 2.98ILYY92 pKa = 10.4AYY94 pKa = 8.32EE95 pKa = 3.98QEE97 pKa = 4.17

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A010ZXF2|A0A010ZXF2_9BACL Histidine kinase OS=Saccharibacillus sacchari DSM 19268 OX=915437 GN=SacsacDRAFT_0280 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 10.49AKK4 pKa = 10.53VIVVKK9 pKa = 10.57VLAGLVLLGYY19 pKa = 10.55GGLLLYY25 pKa = 10.36WMFWGFGRR33 pKa = 11.84SAHH36 pKa = 6.19ISGDD40 pKa = 3.03FRR42 pKa = 11.84YY43 pKa = 10.63NIVPFEE49 pKa = 4.29TIQLFARR56 pKa = 11.84SASWDD61 pKa = 3.57NLRR64 pKa = 11.84APLINLAGNVAVFVPFGVLFPILFGKK90 pKa = 8.98CRR92 pKa = 11.84NYY94 pKa = 10.61FGFLTRR100 pKa = 11.84FLLFIVILEE109 pKa = 4.43LAQGVLGAGVADD121 pKa = 3.81VDD123 pKa = 4.96DD124 pKa = 5.4VILNSIGASLGYY136 pKa = 9.69IGYY139 pKa = 9.95RR140 pKa = 11.84LIAGPEE146 pKa = 3.74QKK148 pKa = 10.47RR149 pKa = 11.84NGRR152 pKa = 11.84AHH154 pKa = 6.7ARR156 pKa = 11.84TRR158 pKa = 11.84SANARR163 pKa = 11.84SRR165 pKa = 11.84SGQEE169 pKa = 3.26RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 3.69

Molecular weight: 19.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

299

0

299

94455

32

1443

315.9

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.385 ± 0.184

0.66 ± 0.042

5.497 ± 0.084

7.94 ± 0.17

3.658 ± 0.088

7.891 ± 0.129

1.952 ± 0.075

5.995 ± 0.101

4.956 ± 0.124

9.825 ± 0.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.078

3.318 ± 0.086

3.994 ± 0.104

3.986 ± 0.115

5.93 ± 0.134

6.188 ± 0.106

5.268 ± 0.101

6.986 ± 0.097

0.925 ± 0.056

2.827 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski