Streptococcus satellite phage Javan743
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZZZ2|A0A4D5ZZZ2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan743 OX=2558837 GN=JavanS743_0024 PE=4 SV=1
MM1 pKa = 7.87 EE2 pKa = 4.51 EE3 pKa = 3.56 TTYY6 pKa = 11.03 DD7 pKa = 3.82 IIAKK11 pKa = 9.74 SLDD14 pKa = 4.0 RR15 pKa = 11.84 ISMEE19 pKa = 3.74 LHH21 pKa = 5.91 QADD24 pKa = 4.33 EE25 pKa = 4.59 NNDD28 pKa = 3.14 FLEE31 pKa = 4.3 YY32 pKa = 10.95 GFYY35 pKa = 10.31 QDD37 pKa = 3.87 NN38 pKa = 3.58
Molecular weight: 4.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A4D5ZV45|A0A4D5ZV45_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan743 OX=2558837 GN=JavanS743_0017 PE=4 SV=1
MM1 pKa = 7.37 TNYY4 pKa = 10.27 DD5 pKa = 3.45 RR6 pKa = 11.84 AKK8 pKa = 10.18 RR9 pKa = 11.84 FYY11 pKa = 11.3 SRR13 pKa = 11.84 ATYY16 pKa = 10.2 VLSDD20 pKa = 3.99 FLHH23 pKa = 6.11 GRR25 pKa = 11.84 IDD27 pKa = 3.42 EE28 pKa = 4.27 QEE30 pKa = 4.16 FRR32 pKa = 11.84 KK33 pKa = 9.32 QWQRR37 pKa = 11.84 LADD40 pKa = 3.58 KK41 pKa = 10.66 AVNEE45 pKa = 4.4 TKK47 pKa = 10.79 KK48 pKa = 10.85 NN49 pKa = 3.51
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.069
IPC2_protein 9.224
IPC_protein 9.326
Toseland 9.882
ProMoST 9.589
Dawson 10.101
Bjellqvist 9.765
Wikipedia 10.277
Rodwell 10.452
Grimsley 10.189
Solomon 10.16
Lehninger 10.131
Nozaki 9.838
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.292
Sillero 10.014
Patrickios 10.16
IPC_peptide 10.16
IPC2_peptide 8.317
IPC2.peptide.svr19 8.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29
0
29
4434
38
496
152.9
17.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.074 ± 0.295
0.699 ± 0.153
6.089 ± 0.329
8.683 ± 0.532
4.42 ± 0.289
5.074 ± 0.389
1.511 ± 0.181
6.969 ± 0.394
8.931 ± 0.495
9.968 ± 0.424
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.797 ± 0.276
5.39 ± 0.41
2.616 ± 0.3
4.06 ± 0.291
5.774 ± 0.37
5.616 ± 0.349
5.909 ± 0.574
5.097 ± 0.335
0.88 ± 0.194
4.443 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here