Glaciibacter sp. YIM 131861

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Glaciibacter

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4FZ50|A0A4S4FZ50_9MICO HAD family hydrolase OS=Glaciibacter sp. YIM 131861 OX=2565934 GN=E6C70_00195 PE=4 SV=1
MM1 pKa = 7.44SLFITCPVEE10 pKa = 4.1SVEE13 pKa = 4.5RR14 pKa = 11.84ATAFYY19 pKa = 8.82TALGWTLNTDD29 pKa = 3.35MSDD32 pKa = 3.56HH33 pKa = 6.15NVSCFAVAPDD43 pKa = 3.65QYY45 pKa = 11.84VMLGSRR51 pKa = 11.84EE52 pKa = 3.98MYY54 pKa = 10.38ASVGATEE61 pKa = 4.31EE62 pKa = 4.37MVGGPDD68 pKa = 3.38TPSKK72 pKa = 9.78VTVSFDD78 pKa = 3.2LGSRR82 pKa = 11.84EE83 pKa = 4.16AVDD86 pKa = 3.71EE87 pKa = 4.21LVDD90 pKa = 3.54RR91 pKa = 11.84AGAAGGRR98 pKa = 11.84VGDD101 pKa = 3.73TDD103 pKa = 5.49DD104 pKa = 3.91YY105 pKa = 11.22PFMYY109 pKa = 9.82QRR111 pKa = 11.84QFDD114 pKa = 4.23DD115 pKa = 3.67PDD117 pKa = 4.88GYY119 pKa = 10.83HH120 pKa = 6.7YY121 pKa = 11.36SPFWMKK127 pKa = 10.75PDD129 pKa = 3.74TDD131 pKa = 5.01PIAA134 pKa = 5.07

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4FX21|A0A4S4FX21_9MICO GNAT family N-acetyltransferase OS=Glaciibacter sp. YIM 131861 OX=2565934 GN=E6C70_07825 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84THH5 pKa = 6.02RR6 pKa = 11.84TQLVLTTLLLGEE18 pKa = 4.43FLLLVVGVTIAARR31 pKa = 11.84RR32 pKa = 11.84GASARR37 pKa = 11.84PQPFSTTIVALLPRR51 pKa = 11.84LPLATIPARR60 pKa = 11.84RR61 pKa = 11.84SPITTLIRR69 pKa = 11.84LPVTITARR77 pKa = 11.84RR78 pKa = 11.84TRR80 pKa = 11.84GRR82 pKa = 11.84AITLLPRR89 pKa = 11.84LPLTIPTRR97 pKa = 11.84SRR99 pKa = 11.84AIPTLVRR106 pKa = 11.84LTVAITARR114 pKa = 11.84RR115 pKa = 11.84TRR117 pKa = 11.84GRR119 pKa = 11.84AIALLPRR126 pKa = 11.84LPLTTITTGRR136 pKa = 11.84TRR138 pKa = 11.84GGAIALLPRR147 pKa = 11.84LPLATVPTGRR157 pKa = 11.84SPITTLIRR165 pKa = 11.84LTVAITTRR173 pKa = 11.84RR174 pKa = 11.84TRR176 pKa = 11.84RR177 pKa = 11.84SGTITLLPRR186 pKa = 11.84LPLPTITTGCGAIPTLIRR204 pKa = 11.84LTVTVTARR212 pKa = 11.84RR213 pKa = 11.84TRR215 pKa = 11.84RR216 pKa = 11.84GGTIPLLPWLPLTIGTRR233 pKa = 11.84PTTITTFVGLAVAIAARR250 pKa = 11.84RR251 pKa = 11.84TRR253 pKa = 11.84GRR255 pKa = 11.84PIALLPRR262 pKa = 11.84LPLTIATRR270 pKa = 11.84RR271 pKa = 11.84AAAARR276 pKa = 11.84RR277 pKa = 11.84TGVALLVGLALGPLPARR294 pKa = 11.84FARR297 pKa = 11.84PAGVGSLLPGLSVVALSGTCRR318 pKa = 11.84GEE320 pKa = 3.86IAHH323 pKa = 6.46GVHH326 pKa = 6.57LLVSAVATRR335 pKa = 11.84HH336 pKa = 5.18YY337 pKa = 10.8LPAGGSAVVLSGTQMRR353 pKa = 4.39

Molecular weight: 37.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3119

0

3119

990533

21

1964

317.6

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.37 ± 0.055

0.483 ± 0.008

6.39 ± 0.04

5.254 ± 0.045

3.175 ± 0.025

8.932 ± 0.038

1.937 ± 0.022

4.761 ± 0.03

2.028 ± 0.028

9.942 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.784 ± 0.017

2.03 ± 0.026

5.287 ± 0.03

2.695 ± 0.023

7.212 ± 0.053

6.123 ± 0.033

6.08 ± 0.044

9.009 ± 0.041

1.506 ± 0.018

2.003 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski