Geobacillus sp. (strain Y412MC10)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3EAN0|D3EAN0_GEOS4 Major facilitator superfamily MFS_1 OS=Geobacillus sp. (strain Y412MC10) OX=481743 GN=GYMC10_0458 PE=4 SV=1
MM1 pKa = 7.89AKK3 pKa = 9.0FTWVDD8 pKa = 2.8KK9 pKa = 9.28DD10 pKa = 3.45TCIACGACGATAPDD24 pKa = 3.47IYY26 pKa = 11.02DD27 pKa = 3.64YY28 pKa = 11.51DD29 pKa = 4.43DD30 pKa = 3.97EE31 pKa = 5.79GLAEE35 pKa = 4.55VIFDD39 pKa = 3.58GDD41 pKa = 3.41NNRR44 pKa = 11.84GVTEE48 pKa = 4.22IPEE51 pKa = 4.35DD52 pKa = 3.54MHH54 pKa = 9.58DD55 pKa = 5.44DD56 pKa = 3.71MLDD59 pKa = 3.59ACDD62 pKa = 4.25GCPTDD67 pKa = 4.53SIRR70 pKa = 11.84IADD73 pKa = 3.78EE74 pKa = 4.1PFNAEE79 pKa = 3.64

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3EJG4|D3EJG4_GEOS4 Two component transcriptional regulator winged helix family OS=Geobacillus sp. (strain Y412MC10) OX=481743 GN=GYMC10_1350 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLANRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 8.54VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6237

0

6237

2028501

30

2528

325.2

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.076 ± 0.037

0.723 ± 0.01

5.238 ± 0.024

6.874 ± 0.032

4.13 ± 0.024

7.591 ± 0.032

2.206 ± 0.017

6.599 ± 0.029

5.073 ± 0.029

10.035 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.017 ± 0.015

3.72 ± 0.021

4.189 ± 0.019

3.83 ± 0.016

5.133 ± 0.028

6.33 ± 0.022

5.277 ± 0.029

6.966 ± 0.025

1.382 ± 0.014

3.611 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski