Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus acidophilus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1859 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5FLF7|Q5FLF7_LACAC Ribokinase OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=rbsK PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.46ARR4 pKa = 11.84IVYY7 pKa = 10.79ASMTGNDD14 pKa = 3.66EE15 pKa = 4.22DD16 pKa = 3.82MADD19 pKa = 3.5ILEE22 pKa = 4.96EE23 pKa = 4.3DD24 pKa = 3.63LQDD27 pKa = 3.43YY28 pKa = 10.42GFDD31 pKa = 3.71VEE33 pKa = 4.45TSDD36 pKa = 5.32VGFTDD41 pKa = 3.64ASDD44 pKa = 3.95YY45 pKa = 11.12LDD47 pKa = 3.89SDD49 pKa = 3.32ICIFITYY56 pKa = 8.86TYY58 pKa = 11.03GEE60 pKa = 4.17GAMTDD65 pKa = 3.65EE66 pKa = 4.84LADD69 pKa = 4.23FYY71 pKa = 11.8EE72 pKa = 4.29EE73 pKa = 4.65LKK75 pKa = 11.17KK76 pKa = 10.76LDD78 pKa = 3.68LSGKK82 pKa = 8.54YY83 pKa = 8.86FAVMGSGDD91 pKa = 3.36KK92 pKa = 10.61TYY94 pKa = 11.3GEE96 pKa = 4.92HH97 pKa = 5.53YY98 pKa = 9.84CEE100 pKa = 4.26NVFDD104 pKa = 4.35FEE106 pKa = 5.32KK107 pKa = 11.18AFLDD111 pKa = 3.7CHH113 pKa = 5.42ATEE116 pKa = 3.69ITKK119 pKa = 10.13PVTIEE124 pKa = 3.79NAPDD128 pKa = 4.18DD129 pKa = 4.36DD130 pKa = 7.47AIDD133 pKa = 5.55LIDD136 pKa = 3.91NAAKK140 pKa = 10.44EE141 pKa = 4.23MADD144 pKa = 3.9KK145 pKa = 11.42LNDD148 pKa = 3.37

Molecular weight: 16.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5FHQ8|Q5FHQ8_LACAC 6-phosphogluconate dehydrogenase decarboxylating OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=LBA1973 PE=3 SV=1
MM1 pKa = 6.05TTKK4 pKa = 9.88RR5 pKa = 11.84TYY7 pKa = 9.64QPKK10 pKa = 8.89KK11 pKa = 7.38RR12 pKa = 11.84HH13 pKa = 5.81RR14 pKa = 11.84SRR16 pKa = 11.84VHH18 pKa = 6.84GFMKK22 pKa = 10.5RR23 pKa = 11.84MSTSNGRR30 pKa = 11.84KK31 pKa = 8.23VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.94GRR41 pKa = 11.84KK42 pKa = 8.67VLSAA46 pKa = 4.05

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1859

0

1859

583994

27

4326

314.1

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.05 ± 0.074

0.541 ± 0.015

6.129 ± 0.065

5.709 ± 0.061

4.309 ± 0.056

6.422 ± 0.052

2.023 ± 0.023

7.813 ± 0.073

8.001 ± 0.062

9.048 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.043

5.845 ± 0.063

3.562 ± 0.041

3.803 ± 0.036

3.695 ± 0.044

5.891 ± 0.04

5.791 ± 0.117

6.767 ± 0.049

1.035 ± 0.019

3.891 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski