Nostoc sp. PCC 7107

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; unclassified Nostoc

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9QBW6|K9QBW6_9NOSO Histidine kinase OS=Nostoc sp. PCC 7107 OX=317936 GN=Nos7107_2027 PE=4 SV=1
MM1 pKa = 6.68TTIPSDD7 pKa = 3.72PLFPNQWYY15 pKa = 9.91LYY17 pKa = 9.78NQGQSGGTPGIDD29 pKa = 2.91LNVVNVWEE37 pKa = 5.15DD38 pKa = 3.35YY39 pKa = 8.86TGRR42 pKa = 11.84GVVVGVIDD50 pKa = 4.99DD51 pKa = 4.25GFDD54 pKa = 3.34YY55 pKa = 10.59THH57 pKa = 7.09YY58 pKa = 11.3DD59 pKa = 3.33LDD61 pKa = 4.04NNYY64 pKa = 7.99DD65 pKa = 3.43TTRR68 pKa = 11.84DD69 pKa = 3.42YY70 pKa = 11.31DD71 pKa = 4.0TIEE74 pKa = 4.02QNFDD78 pKa = 3.37PFGNAIDD85 pKa = 3.69SHH87 pKa = 5.71GTSVAGIIASEE98 pKa = 4.2ANGVGTVGVAFNATISGIRR117 pKa = 11.84AIGFGGDD124 pKa = 3.26VVGALWRR131 pKa = 11.84AEE133 pKa = 4.05SFDD136 pKa = 3.58VVNNSWGVSGFFADD150 pKa = 3.72NFYY153 pKa = 10.41TIPQAGQAIANAVTNGRR170 pKa = 11.84NGLGTAIVFAAGNSRR185 pKa = 11.84QEE187 pKa = 4.09GDD189 pKa = 2.95NTNYY193 pKa = 10.42HH194 pKa = 6.09NFQNSRR200 pKa = 11.84QVITVAALDD209 pKa = 4.03HH210 pKa = 6.72NGFASPYY217 pKa = 7.46STPGASILVSAFGSGYY233 pKa = 10.48DD234 pKa = 3.76GSIVTTDD241 pKa = 3.14RR242 pKa = 11.84QGSDD246 pKa = 2.96GYY248 pKa = 11.37SFEE251 pKa = 5.13DD252 pKa = 3.42YY253 pKa = 10.67NYY255 pKa = 10.78SFNGTSAAAPQVSGVVALLLEE276 pKa = 4.61ANRR279 pKa = 11.84NLGYY283 pKa = 10.3RR284 pKa = 11.84DD285 pKa = 3.26IQEE288 pKa = 3.85ILAYY292 pKa = 9.4SARR295 pKa = 11.84QNDD298 pKa = 4.75FYY300 pKa = 11.6NQGGNYY306 pKa = 8.18IWQINGANNFNGGGLHH322 pKa = 6.22VSHH325 pKa = 8.04DD326 pKa = 3.98YY327 pKa = 11.66GFGLVDD333 pKa = 3.1AHH335 pKa = 6.37AAVRR339 pKa = 11.84LAEE342 pKa = 4.16TWQKK346 pKa = 11.04QSVFSNEE353 pKa = 3.39QSLSYY358 pKa = 11.01SSGNLGLVIPDD369 pKa = 4.02SNTGGISSTFTVAAGLDD386 pKa = 3.8VDD388 pKa = 3.86WVEE391 pKa = 4.25VEE393 pKa = 4.73LNLTHH398 pKa = 7.29SYY400 pKa = 10.71RR401 pKa = 11.84GDD403 pKa = 3.57LVVDD407 pKa = 5.54LISPSGTISRR417 pKa = 11.84LINQPGDD424 pKa = 3.14GWDD427 pKa = 3.35SGDD430 pKa = 3.67NIVFKK435 pKa = 10.87LSSSQHH441 pKa = 5.28WGEE444 pKa = 4.07SSAGNWTLRR453 pKa = 11.84IQDD456 pKa = 4.71LFLSDD461 pKa = 3.64TGILNSWNLRR471 pKa = 11.84LYY473 pKa = 11.15GDD475 pKa = 3.91TDD477 pKa = 3.93TANDD481 pKa = 3.71TYY483 pKa = 10.93FYY485 pKa = 8.94TNEE488 pKa = 3.65YY489 pKa = 9.88GFYY492 pKa = 10.03GTTTPSDD499 pKa = 3.37NTGTDD504 pKa = 3.67TINAAAITSNSYY516 pKa = 11.36LDD518 pKa = 4.58LNAGSTSILNGTTLIINTGTIIEE541 pKa = 4.29NAFGGDD547 pKa = 3.62GNDD550 pKa = 3.74TIIGNSAANVLSGGRR565 pKa = 11.84GDD567 pKa = 5.05DD568 pKa = 3.99SLDD571 pKa = 3.53GGAGNDD577 pKa = 3.64TLRR580 pKa = 11.84GGRR583 pKa = 11.84DD584 pKa = 2.99NDD586 pKa = 3.92TYY588 pKa = 11.13IVNTSGDD595 pKa = 3.85VVIEE599 pKa = 3.99NANEE603 pKa = 4.59GIDD606 pKa = 3.88TIRR609 pKa = 11.84TSISYY614 pKa = 8.57TLGANLEE621 pKa = 4.04NLILTGTAAINGTGNTLNNSITGNSGNNTLNGGVGNDD658 pKa = 3.93TLNGGAGNDD667 pKa = 3.73TYY669 pKa = 11.14TVDD672 pKa = 3.67SIGDD676 pKa = 3.69VVIEE680 pKa = 4.12NANEE684 pKa = 3.94GTDD687 pKa = 3.75TIRR690 pKa = 11.84TSISYY695 pKa = 8.62TLGSNLEE702 pKa = 3.95NLILTGTAAINGTGNTLNNSITGNSGNNTLNGGAGNDD739 pKa = 3.73TLTAGAGNDD748 pKa = 3.65TLTGGLGRR756 pKa = 11.84DD757 pKa = 3.08SFNFNSRR764 pKa = 11.84TEE766 pKa = 4.46GIDD769 pKa = 3.41RR770 pKa = 11.84ITDD773 pKa = 3.8FSVIDD778 pKa = 3.5DD779 pKa = 4.61TIFVSASGFGGGLVTGAAIAANRR802 pKa = 11.84FVIGAAATTSSQRR815 pKa = 11.84FIYY818 pKa = 10.88NNVTGGLFFDD828 pKa = 4.87ADD830 pKa = 3.73GTGAIARR837 pKa = 11.84IQFATLSAGLSLTNADD853 pKa = 3.29IFVNTT858 pKa = 4.48

Molecular weight: 89.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9QFD9|K9QFD9_9NOSO Formyltetrahydrofolate deformylase OS=Nostoc sp. PCC 7107 OX=317936 GN=purU PE=3 SV=1
MM1 pKa = 7.12SQSSLNRR8 pKa = 11.84RR9 pKa = 11.84LTQAFGILLGIGIAIWILRR28 pKa = 11.84GLGILTFLPGGIIWLFVLGAIALGIISFAQRR59 pKa = 11.84TWWRR63 pKa = 11.84FF64 pKa = 2.98

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5185

0

5185

1692159

31

2335

326.4

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.236 ± 0.037

0.965 ± 0.011

4.785 ± 0.026

6.096 ± 0.032

3.923 ± 0.02

6.508 ± 0.032

1.828 ± 0.015

7.033 ± 0.029

4.844 ± 0.03

11.017 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.787 ± 0.017

4.619 ± 0.033

4.629 ± 0.027

5.623 ± 0.03

4.891 ± 0.027

6.322 ± 0.029

5.8 ± 0.029

6.595 ± 0.028

1.392 ± 0.015

3.108 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski