Streptomyces jeddahensis
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7356 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A177HTM2|A0A177HTM2_9ACTN Uncharacterized protein OS=Streptomyces jeddahensis OX=1716141 GN=STSP_23090 PE=4 SV=1
MM1 pKa = 7.2 QFLAQIILDD10 pKa = 3.99 GQGRR14 pKa = 11.84 PIDD17 pKa = 3.6 QRR19 pKa = 11.84 RR20 pKa = 11.84 TQAGLVMAIFMCQNDD35 pKa = 4.13 PGMCDD40 pKa = 2.9 EE41 pKa = 5.02 WSPTSGGNKK50 pKa = 9.81 ALLFPSDD57 pKa = 4.06 GLQPMPAPEE66 pKa = 4.67 PDD68 pKa = 3.29 EE69 pKa = 5.35 DD70 pKa = 4.31 EE71 pKa = 4.94 GVLHH75 pKa = 6.85 LGAVNAVTLVSLPSADD91 pKa = 3.44 YY92 pKa = 11.06 GSARR96 pKa = 11.84 EE97 pKa = 4.11 EE98 pKa = 3.79 WGSRR102 pKa = 11.84 SGNDD106 pKa = 3.17 LGNVLGQLGGQPDD119 pKa = 4.0 WLQDD123 pKa = 3.93 DD124 pKa = 4.37 EE125 pKa = 5.49 TPTCPTCTQPMDD137 pKa = 4.11 LVAQFEE143 pKa = 4.62 EE144 pKa = 5.68 GPDD147 pKa = 3.09 HH148 pKa = 5.82 ATAMNFGGCGTAFAFACGPCTRR170 pKa = 11.84 AAFLWQCC177 pKa = 3.84
Molecular weight: 18.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 0.998
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A177HML4|A0A177HML4_9ACTN Transcriptional regulator NanR OS=Streptomyces jeddahensis OX=1716141 GN=STSP_52220 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.75 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7356
0
7356
2403687
29
3678
326.8
35.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.385 ± 0.043
0.798 ± 0.009
5.966 ± 0.026
5.697 ± 0.032
2.76 ± 0.017
9.468 ± 0.03
2.283 ± 0.014
3.414 ± 0.02
2.337 ± 0.026
10.098 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.788 ± 0.012
1.872 ± 0.016
5.981 ± 0.026
2.906 ± 0.017
7.784 ± 0.034
5.253 ± 0.023
6.12 ± 0.024
8.359 ± 0.022
1.545 ± 0.013
2.185 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here