Penicillium sp. occitanis
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11231 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H3I571|A0A2H3I571_9EURO Uncharacterized protein OS=Penicillium sp. 'occitanis' OX=290292 GN=PENO1_079590 PE=4 SV=1
MM1 pKa = 7.86 PSLTQVSLFLSLTIPGLVQAVPAPAPTTPPKK32 pKa = 10.35 LSEE35 pKa = 4.09 RR36 pKa = 11.84 ASSCTFSGSQGAASASKK53 pKa = 10.73 SKK55 pKa = 9.52 TSCSTIVLSDD65 pKa = 3.44 VAVPSGTTLDD75 pKa = 3.9 LTDD78 pKa = 5.36 LNDD81 pKa = 3.68 GTSVIFEE88 pKa = 4.31 GTTSFGYY95 pKa = 9.98 EE96 pKa = 3.81 EE97 pKa = 4.47 WDD99 pKa = 3.72 GPLISVSGADD109 pKa = 3.27 ITVTGADD116 pKa = 3.33 GHH118 pKa = 6.8 VIDD121 pKa = 5.78 GDD123 pKa = 4.0 GSRR126 pKa = 11.84 WWDD129 pKa = 3.42 GEE131 pKa = 3.95 GSNGGTTKK139 pKa = 10.62 PKK141 pKa = 10.28 FFYY144 pKa = 10.74 AHH146 pKa = 7.7 DD147 pKa = 4.24 LTSSTISGLSLKK159 pKa = 10.27 NSPVQTFSIDD169 pKa = 2.93 GSTDD173 pKa = 3.11 LTLSDD178 pKa = 3.63 ITIDD182 pKa = 5.56 DD183 pKa = 3.68 SDD185 pKa = 5.41 GDD187 pKa = 4.34 DD188 pKa = 3.62 GSAANTDD195 pKa = 3.33 AFDD198 pKa = 3.59 VGEE201 pKa = 4.22 STGIIISGATVYY213 pKa = 10.98 NQDD216 pKa = 3.5 DD217 pKa = 4.0 CLAINSGTNITFTGGYY233 pKa = 9.4 CSGGHH238 pKa = 5.92 GLSIGSVGGRR248 pKa = 11.84 DD249 pKa = 3.83 DD250 pKa = 3.76 NTVSGVTIEE259 pKa = 4.66 SSTITNSANGVRR271 pKa = 11.84 IKK273 pKa = 9.75 TVYY276 pKa = 10.4 DD277 pKa = 3.22 ATGSVTGVTYY287 pKa = 11.04 KK288 pKa = 10.86 DD289 pKa = 3.11 ITLSGITDD297 pKa = 3.45 YY298 pKa = 11.69 GIVIEE303 pKa = 4.22 QDD305 pKa = 3.68 YY306 pKa = 11.2 EE307 pKa = 4.24 NGSPTGTPTTGVPITDD323 pKa = 3.59 LTIDD327 pKa = 3.64 NVQGTVEE334 pKa = 3.96 SDD336 pKa = 2.77 ATEE339 pKa = 4.04 IYY341 pKa = 9.99 ILCGDD346 pKa = 4.84 GSCSDD351 pKa = 3.67 WTWTDD356 pKa = 3.16 VSVTGGEE363 pKa = 4.19 TSDD366 pKa = 3.54 KK367 pKa = 11.14 CEE369 pKa = 4.17 NAPDD373 pKa = 5.6 DD374 pKa = 4.02 ISCC377 pKa = 4.64
Molecular weight: 38.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.414
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.312
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.062
Thurlkill 3.478
EMBOSS 3.63
Sillero 3.77
Patrickios 0.757
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A2H3I952|A0A2H3I952_9EURO Mitochondrial export protein Som1 OS=Penicillium sp. 'occitanis' OX=290292 GN=PENO1_064200 PE=4 SV=1
MM1 pKa = 7.7 LLRR4 pKa = 11.84 YY5 pKa = 8.21 TEE7 pKa = 4.3 LGSRR11 pKa = 11.84 RR12 pKa = 11.84 TKK14 pKa = 9.22 MMEE17 pKa = 3.85 YY18 pKa = 10.45 VGTQLFWSQSEE29 pKa = 3.84 ARR31 pKa = 11.84 QAGDD35 pKa = 3.17 HH36 pKa = 6.84 RR37 pKa = 11.84 DD38 pKa = 3.21 AKK40 pKa = 9.61 TAAEE44 pKa = 3.73 ARR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 KK50 pKa = 8.79 VQNRR54 pKa = 11.84 KK55 pKa = 6.05 NQRR58 pKa = 11.84 VRR60 pKa = 11.84 RR61 pKa = 11.84 LRR63 pKa = 11.84 LKK65 pKa = 11.0 GGDD68 pKa = 2.87 AGNFQEE74 pKa = 4.82 SRR76 pKa = 11.84 PFRR79 pKa = 11.84 VKK81 pKa = 9.87 RR82 pKa = 11.84 WRR84 pKa = 11.84 LDD86 pKa = 3.59 EE87 pKa = 5.08 PDD89 pKa = 5.31 HH90 pKa = 6.74 IPSSEE95 pKa = 3.89 NSPASEE101 pKa = 3.99 RR102 pKa = 11.84 ATTTAFFHH110 pKa = 6.54 GPPHH114 pKa = 6.84 AGITASAAKK123 pKa = 9.93 GRR125 pKa = 11.84 VVLRR129 pKa = 11.84 EE130 pKa = 3.9 SLSTITHH137 pKa = 5.56 SQPLVNLDD145 pKa = 3.27 IQPFTFPFSSDD156 pKa = 2.43 HH157 pKa = 6.44 HH158 pKa = 6.56 LLHH161 pKa = 7.07 FIHH164 pKa = 6.7 YY165 pKa = 7.53 NVCRR169 pKa = 11.84 ALITNMRR176 pKa = 11.84 TLNTVPADD184 pKa = 3.54 STFCTIASPCRR195 pKa = 11.84 DD196 pKa = 3.8 DD197 pKa = 3.52 TTLFPLKK204 pKa = 10.21 PDD206 pKa = 3.93 IPPSLIPTAFQQTHH220 pKa = 5.32 DD221 pKa = 3.33 HH222 pKa = 6.53 FARR225 pKa = 11.84 IKK227 pKa = 10.45 SFPSLASARR236 pKa = 11.84 II237 pKa = 3.59
Molecular weight: 26.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.516
IPC_protein 10.335
Toseland 10.73
ProMoST 10.452
Dawson 10.804
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 10.921
Grimsley 10.833
Solomon 10.965
Lehninger 10.921
Nozaki 10.716
DTASelect 10.511
Thurlkill 10.716
EMBOSS 11.14
Sillero 10.745
Patrickios 10.657
IPC_peptide 10.965
IPC2_peptide 9.619
IPC2.peptide.svr19 8.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11231
0
11231
5743013
66
7905
511.4
56.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.343 ± 0.019
1.125 ± 0.007
5.732 ± 0.014
6.189 ± 0.022
3.856 ± 0.012
6.674 ± 0.02
2.345 ± 0.009
5.446 ± 0.014
4.768 ± 0.019
9.039 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.144 ± 0.009
3.966 ± 0.011
5.587 ± 0.022
4.012 ± 0.015
5.711 ± 0.019
8.214 ± 0.022
6.162 ± 0.014
6.223 ± 0.014
1.485 ± 0.009
2.979 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here