Staphylococcus phage phi575

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; unclassified Peeveelvirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q1PVT4|A0A1Q1PVT4_9CAUD CI-like repressor with LexA and peptidase domain OS=Staphylococcus phage phi575 OX=1955176 PE=4 SV=1
MM1 pKa = 7.39NIAWNIIKK9 pKa = 10.7YY10 pKa = 8.66PALTLAIVVEE20 pKa = 4.72FFIITSFSTTPIEE33 pKa = 4.02HH34 pKa = 6.88SFLFWIVTVAGFEE47 pKa = 4.02MADD50 pKa = 3.46QIFLEE55 pKa = 4.22QEE57 pKa = 4.21EE58 pKa = 4.48II59 pKa = 3.72

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q1PVW9|A0A1Q1PVW9_9CAUD Head-tail connector protein OS=Staphylococcus phage phi575 OX=1955176 PE=4 SV=1
MM1 pKa = 7.35GGKK4 pKa = 8.79IVKK7 pKa = 8.55NTVAEE12 pKa = 4.23GLKK15 pKa = 10.36RR16 pKa = 11.84EE17 pKa = 4.23LLQMSTLEE25 pKa = 4.04KK26 pKa = 10.38RR27 pKa = 11.84VLKK30 pKa = 10.77AGASTIIPILRR41 pKa = 11.84KK42 pKa = 8.36NTPLGEE48 pKa = 4.3QQRR51 pKa = 11.84HH52 pKa = 4.69ARR54 pKa = 11.84NHH56 pKa = 5.16IAISNVKK63 pKa = 8.47TDD65 pKa = 3.0RR66 pKa = 11.84STLEE70 pKa = 3.77KK71 pKa = 10.19YY72 pKa = 10.15VAVGYY77 pKa = 9.81EE78 pKa = 3.58KK79 pKa = 10.69GYY81 pKa = 7.97SHH83 pKa = 7.75RR84 pKa = 11.84IHH86 pKa = 5.45TTEE89 pKa = 4.4FGTMYY94 pKa = 10.1QRR96 pKa = 11.84PQLFITKK103 pKa = 9.19SEE105 pKa = 4.07KK106 pKa = 9.42EE107 pKa = 3.82SRR109 pKa = 11.84NIVFNAMKK117 pKa = 9.3TAMKK121 pKa = 10.3RR122 pKa = 11.84GLRR125 pKa = 3.75

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12818

51

1656

221.0

25.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.344 ± 0.413

0.694 ± 0.136

5.968 ± 0.333

7.778 ± 0.617

4.252 ± 0.24

5.703 ± 0.659

1.841 ± 0.16

7.724 ± 0.288

9.822 ± 0.435

8.324 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.205

6.686 ± 0.248

2.606 ± 0.162

3.682 ± 0.26

4.002 ± 0.224

6.397 ± 0.351

5.703 ± 0.338

5.773 ± 0.258

1.038 ± 0.127

4.158 ± 0.331
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski