Paraburkholderia eburnea

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S4M8G8|A0A2S4M8G8_9BURK Undecaprenyl-phosphate glucose phosphotransferase OS=Paraburkholderia eburnea OX=1189126 GN=B0G62_10764 PE=3 SV=1
MM1 pKa = 6.76GASYY5 pKa = 10.88ISVTDD10 pKa = 3.44NAASYY15 pKa = 9.52TLDD18 pKa = 3.09NTTFDD23 pKa = 3.97VQSGAGMTLYY33 pKa = 10.8GSSDD37 pKa = 3.65LVFLEE42 pKa = 4.64SGSGIPFGTQGYY54 pKa = 9.14IRR56 pKa = 11.84IVGSGNTVLGSSANGAEE73 pKa = 4.14IVVSGSDD80 pKa = 3.39NVISAGANSVIIDD93 pKa = 3.7GDD95 pKa = 3.53AGGGHH100 pKa = 5.81TLSGNGQNDD109 pKa = 4.61TITVGVNSNVTAGVNSRR126 pKa = 11.84INMTGGYY133 pKa = 10.55AFVATDD139 pKa = 3.47ASAIVSGSSDD149 pKa = 3.49SIEE152 pKa = 3.74AHH154 pKa = 5.96GAVSVAGSNNNIYY167 pKa = 10.72DD168 pKa = 3.46RR169 pKa = 11.84GIGVGAVTISGTSNTVSVGSNRR191 pKa = 11.84LVDD194 pKa = 4.16DD195 pKa = 3.76SQGRR199 pKa = 11.84GDD201 pKa = 5.89LITIGDD207 pKa = 3.95GNQIVLGSGGTLNINGSNNSVSFNTANGAEE237 pKa = 4.45TIQADD242 pKa = 3.0GGYY245 pKa = 9.35FVKK248 pKa = 10.51EE249 pKa = 4.05DD250 pKa = 3.3ASGNISMNASSFAVQNGVALIGLGNGNVVTVSNMKK285 pKa = 10.35SGLGADD291 pKa = 3.47TTANNQVSQLVSAMASYY308 pKa = 10.96SGGAAGITSTVAAQAPVEE326 pKa = 4.04ASLFASAHH334 pKa = 4.12QQ335 pKa = 3.55

Molecular weight: 32.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S4LSI7|A0A2S4LSI7_9BURK 2-methylisocitrate lyase-like PEP mutase family enzyme OS=Paraburkholderia eburnea OX=1189126 GN=B0G62_1349 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6148

0

6148

2001719

25

5337

325.6

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.229 ± 0.044

0.918 ± 0.009

5.433 ± 0.026

5.103 ± 0.03

3.678 ± 0.02

8.205 ± 0.029

2.363 ± 0.015

4.57 ± 0.024

2.918 ± 0.024

10.223 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.015

2.803 ± 0.021

5.071 ± 0.029

3.718 ± 0.018

6.97 ± 0.036

5.6 ± 0.027

5.416 ± 0.023

7.64 ± 0.024

1.416 ± 0.011

2.432 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski