Salmonella phage vB_SpuP_Spp16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Panjvirus; Salmonella virus Spp16

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P9JZR7|A0A2P9JZR7_9CAUD Metallophos domain-containing protein OS=Salmonella phage vB_SpuP_Spp16 OX=2081603 PE=4 SV=1
MM1 pKa = 5.84MHH3 pKa = 6.59KK4 pKa = 10.6VEE6 pKa = 4.52VFEE9 pKa = 6.3DD10 pKa = 3.49NDD12 pKa = 3.52NEE14 pKa = 4.27MVKK17 pKa = 10.17IVVNGEE23 pKa = 4.13MVFHH27 pKa = 6.85GNTWDD32 pKa = 3.18IHH34 pKa = 5.93EE35 pKa = 5.04DD36 pKa = 2.93TWISILSKK44 pKa = 10.73CGVLVITSEE53 pKa = 3.95YY54 pKa = 9.87SYY56 pKa = 11.27SYY58 pKa = 10.57EE59 pKa = 3.86

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346RPD2|A0A346RPD2_9CAUD Holin OS=Salmonella phage vB_SpuP_Spp16 OX=2081603 PE=4 SV=1
MM1 pKa = 7.56QGGLTLGVSVSTISLFGVPLNEE23 pKa = 3.48WVYY26 pKa = 10.74ILTIIILLTQLIRR39 pKa = 11.84TVVNVFIKK47 pKa = 10.11IRR49 pKa = 11.84RR50 pKa = 11.84GICQKK55 pKa = 10.73NN56 pKa = 3.02

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12985

37

1136

245.0

27.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.493 ± 0.543

1.086 ± 0.188

6.038 ± 0.148

7.054 ± 0.302

3.442 ± 0.194

6.669 ± 0.25

1.841 ± 0.192

5.63 ± 0.341

6.946 ± 0.272

8.286 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.205

5.221 ± 0.326

3.704 ± 0.206

4.29 ± 0.315

5.399 ± 0.228

6.03 ± 0.252

5.737 ± 0.239

6.777 ± 0.243

1.224 ± 0.11

4.359 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski