Festuca pratensis amalgavirus 2
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIN1|A0A2Z6JIN1_9VIRU ORF1p OS=Festuca pratensis amalgavirus 2 OX=2069325 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.35 SVDD4 pKa = 3.53 PLRR7 pKa = 11.84 FLEE10 pKa = 4.96 DD11 pKa = 2.99 AATAQAADD19 pKa = 4.45 AIKK22 pKa = 10.42 LRR24 pKa = 11.84 PLMLEE29 pKa = 4.08 LSGSKK34 pKa = 10.2 VLAKK38 pKa = 10.48 EE39 pKa = 3.82 CDD41 pKa = 3.26 IEE43 pKa = 4.27 HH44 pKa = 7.07 LLNAGFTVDD53 pKa = 5.38 DD54 pKa = 4.04 VEE56 pKa = 5.02 KK57 pKa = 10.58 LSKK60 pKa = 10.3 SLKK63 pKa = 9.46 PLIDD67 pKa = 3.1 QGVFDD72 pKa = 5.69 DD73 pKa = 4.28 AWTMAKK79 pKa = 10.2 GSGIILSAQEE89 pKa = 3.87 MTFPDD94 pKa = 3.71 LFMFRR99 pKa = 11.84 RR100 pKa = 11.84 WLTTPQGAQALSLVQARR117 pKa = 11.84 RR118 pKa = 11.84 KK119 pKa = 5.79 MTKK122 pKa = 9.78 AGKK125 pKa = 9.67 KK126 pKa = 9.5 VLGHH130 pKa = 6.0 QDD132 pKa = 3.07 VALLRR137 pKa = 11.84 LLQHH141 pKa = 6.36 YY142 pKa = 9.86 EE143 pKa = 3.52 DD144 pKa = 4.15 DD145 pKa = 4.23 ARR147 pKa = 11.84 RR148 pKa = 11.84 EE149 pKa = 3.75 LDD151 pKa = 3.14 AKK153 pKa = 10.49 RR154 pKa = 11.84 VEE156 pKa = 4.3 TEE158 pKa = 3.62 AAVAQLQAEE167 pKa = 4.09 IDD169 pKa = 3.8 KK170 pKa = 11.04 LKK172 pKa = 10.82 KK173 pKa = 10.28 KK174 pKa = 9.34 YY175 pKa = 10.74 AKK177 pKa = 10.31 AEE179 pKa = 4.13 KK180 pKa = 9.31 KK181 pKa = 9.8 QKK183 pKa = 10.41 RR184 pKa = 11.84 DD185 pKa = 3.37 FPLIANYY192 pKa = 9.96 VPLTDD197 pKa = 3.91 NEE199 pKa = 4.19 VRR201 pKa = 11.84 NQAWDD206 pKa = 3.9 MYY208 pKa = 9.91 CQQCINEE215 pKa = 4.36 GSVPMGRR222 pKa = 11.84 TSTNLKK228 pKa = 9.96 IVGDD232 pKa = 3.93 KK233 pKa = 10.78 FRR235 pKa = 11.84 DD236 pKa = 4.02 HH237 pKa = 7.06 IVQAHH242 pKa = 6.75 KK243 pKa = 9.63 LTYY246 pKa = 9.67 CQQPDD251 pKa = 3.42 HH252 pKa = 6.87 TDD254 pKa = 2.92 ALIEE258 pKa = 4.24 FGKK261 pKa = 10.49 QKK263 pKa = 10.34 ILRR266 pKa = 11.84 CQEE269 pKa = 4.0 AGSGKK274 pKa = 10.31 LEE276 pKa = 3.95 SSFRR280 pKa = 11.84 NLLTVLNPEE289 pKa = 4.22 NAATLPPRR297 pKa = 11.84 TEE299 pKa = 3.92 EE300 pKa = 4.38 ADD302 pKa = 3.81 DD303 pKa = 5.18 GVDD306 pKa = 3.44 TDD308 pKa = 4.68 GGSPNPPEE316 pKa = 5.17 PDD318 pKa = 2.95 THH320 pKa = 8.17 ADD322 pKa = 3.53 AEE324 pKa = 4.36 PAADD328 pKa = 3.61 QPTPPEE334 pKa = 3.8 ATGPGSSAGPTPPQSSRR351 pKa = 11.84 SHH353 pKa = 5.98 KK354 pKa = 9.89 RR355 pKa = 11.84 KK356 pKa = 9.63 QPAVDD361 pKa = 3.79 AQSEE365 pKa = 4.38 DD366 pKa = 3.76 PNPSKK371 pKa = 10.72 KK372 pKa = 10.08 RR373 pKa = 11.84 STRR376 pKa = 11.84 AGRR379 pKa = 11.84 HH380 pKa = 4.91 PNRR383 pKa = 11.84 AEE385 pKa = 3.69
Molecular weight: 42.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.101
IPC2_protein 5.982
IPC_protein 6.059
Toseland 6.415
ProMoST 6.275
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.338
Grimsley 6.547
Solomon 6.364
Lehninger 6.351
Nozaki 6.62
DTASelect 6.81
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.737
Patrickios 4.724
IPC_peptide 6.376
IPC2_peptide 6.605
IPC2.peptide.svr19 6.699
Protein with the highest isoelectric point:
>tr|A0A2Z6JIN1|A0A2Z6JIN1_9VIRU ORF1p OS=Festuca pratensis amalgavirus 2 OX=2069325 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.35 SVDD4 pKa = 3.53 PLRR7 pKa = 11.84 FLEE10 pKa = 4.96 DD11 pKa = 2.99 AATAQAADD19 pKa = 4.45 AIKK22 pKa = 10.42 LRR24 pKa = 11.84 PLMLEE29 pKa = 4.08 LSGSKK34 pKa = 10.2 VLAKK38 pKa = 10.48 EE39 pKa = 3.82 CDD41 pKa = 3.26 IEE43 pKa = 4.27 HH44 pKa = 7.07 LLNAGFTVDD53 pKa = 5.38 DD54 pKa = 4.04 VEE56 pKa = 5.02 KK57 pKa = 10.58 LSKK60 pKa = 10.3 SLKK63 pKa = 9.46 PLIDD67 pKa = 3.1 QGVFDD72 pKa = 5.69 DD73 pKa = 4.28 AWTMAKK79 pKa = 10.2 GSGIILSAQEE89 pKa = 3.87 MTFPDD94 pKa = 3.71 LFMFRR99 pKa = 11.84 RR100 pKa = 11.84 WLTTPQGAQALSLVQARR117 pKa = 11.84 RR118 pKa = 11.84 KK119 pKa = 5.79 MTKK122 pKa = 9.78 AGKK125 pKa = 9.67 KK126 pKa = 9.5 VLGHH130 pKa = 6.0 QDD132 pKa = 3.07 VALLRR137 pKa = 11.84 LLQHH141 pKa = 6.36 YY142 pKa = 9.86 EE143 pKa = 3.52 DD144 pKa = 4.15 DD145 pKa = 4.23 ARR147 pKa = 11.84 RR148 pKa = 11.84 EE149 pKa = 3.75 LDD151 pKa = 3.14 AKK153 pKa = 10.49 RR154 pKa = 11.84 VEE156 pKa = 4.3 TEE158 pKa = 3.62 AAVAQLQAEE167 pKa = 4.09 IDD169 pKa = 3.8 KK170 pKa = 11.04 LKK172 pKa = 10.82 KK173 pKa = 10.28 KK174 pKa = 9.34 YY175 pKa = 10.74 AKK177 pKa = 10.31 AEE179 pKa = 4.13 KK180 pKa = 9.31 KK181 pKa = 9.8 QKK183 pKa = 10.41 RR184 pKa = 11.84 DD185 pKa = 3.37 FPLIANYY192 pKa = 9.96 VPLTDD197 pKa = 3.91 NEE199 pKa = 4.19 VRR201 pKa = 11.84 NQAWDD206 pKa = 3.9 MYY208 pKa = 9.91 CQQCINEE215 pKa = 4.36 GSVPMGRR222 pKa = 11.84 TSTNLKK228 pKa = 9.96 IVGDD232 pKa = 3.93 KK233 pKa = 10.78 FRR235 pKa = 11.84 DD236 pKa = 4.02 HH237 pKa = 7.06 IVQAHH242 pKa = 6.75 KK243 pKa = 9.63 LTYY246 pKa = 9.67 CQQPDD251 pKa = 3.42 HH252 pKa = 6.87 TDD254 pKa = 2.92 ALIEE258 pKa = 4.24 FGKK261 pKa = 10.49 QKK263 pKa = 10.34 ILRR266 pKa = 11.84 CQEE269 pKa = 4.0 AGSGKK274 pKa = 10.27 LEE276 pKa = 4.28 SSFVTYY282 pKa = 10.46 SLSSIQRR289 pKa = 11.84 MLLRR293 pKa = 11.84 YY294 pKa = 9.0 PLAQRR299 pKa = 11.84 KK300 pKa = 7.47 QMMEE304 pKa = 5.05 WIPMGVVPTRR314 pKa = 11.84 PSLTRR319 pKa = 11.84 TQMLSQLPTNPLLLKK334 pKa = 10.39 PRR336 pKa = 11.84 VLGARR341 pKa = 11.84 PGPPLPKK348 pKa = 9.97 AVEE351 pKa = 4.67 AISGNSLLSTPNRR364 pKa = 11.84 RR365 pKa = 11.84 IQIHH369 pKa = 6.03 RR370 pKa = 11.84 RR371 pKa = 11.84 SGQPGQAGIPTARR384 pKa = 11.84 SRR386 pKa = 11.84 WEE388 pKa = 3.56 AGVRR392 pKa = 11.84 HH393 pKa = 6.67 IIGGGEE399 pKa = 3.93 LLEE402 pKa = 4.09 WRR404 pKa = 11.84 VDD406 pKa = 3.37 NNKK409 pKa = 9.56 YY410 pKa = 10.29 RR411 pKa = 11.84 GGGNLHH417 pKa = 7.22 DD418 pKa = 5.07 ALLLIGSADD427 pKa = 3.78 DD428 pKa = 3.67 VTPYY432 pKa = 9.98 TALSSLLSVEE442 pKa = 3.9 EE443 pKa = 3.8 ARR445 pKa = 11.84 RR446 pKa = 11.84 VLLLPDD452 pKa = 4.17 GLAVPDD458 pKa = 4.76 GKK460 pKa = 10.69 ACCVMKK466 pKa = 10.53 QFNDD470 pKa = 3.67 DD471 pKa = 3.34 ATAGPLLRR479 pKa = 11.84 AFGVKK484 pKa = 9.83 GKK486 pKa = 10.4 YY487 pKa = 8.78 GLKK490 pKa = 10.22 AAIEE494 pKa = 4.04 QFVWGLYY501 pKa = 10.32 DD502 pKa = 3.56 RR503 pKa = 11.84 VGSEE507 pKa = 4.19 DD508 pKa = 3.63 LKK510 pKa = 11.0 PRR512 pKa = 11.84 QLPGLLARR520 pKa = 11.84 VGYY523 pKa = 7.67 RR524 pKa = 11.84 TKK526 pKa = 10.96 LLDD529 pKa = 3.36 MEE531 pKa = 4.67 KK532 pKa = 10.61 ALKK535 pKa = 9.94 KK536 pKa = 9.72 IQAVEE541 pKa = 3.78 PLGRR545 pKa = 11.84 AVMMLDD551 pKa = 3.04 ATEE554 pKa = 4.19 QCFSSPLFNAISEE567 pKa = 4.55 AVTEE571 pKa = 4.06 LHH573 pKa = 6.54 SNPRR577 pKa = 11.84 SGWRR581 pKa = 11.84 NYY583 pKa = 9.62 LVRR586 pKa = 11.84 ASSAWADD593 pKa = 3.14 LWDD596 pKa = 3.9 EE597 pKa = 4.57 LKK599 pKa = 10.87 SCGTIVEE606 pKa = 4.5 LDD608 pKa = 3.02 WSKK611 pKa = 11.06 FDD613 pKa = 4.01 RR614 pKa = 11.84 DD615 pKa = 3.77 RR616 pKa = 11.84 PAEE619 pKa = 4.6 DD620 pKa = 2.75 IQFFVDD626 pKa = 4.62 VIISCFRR633 pKa = 11.84 PKK635 pKa = 10.44 NSRR638 pKa = 11.84 EE639 pKa = 3.63 RR640 pKa = 11.84 RR641 pKa = 11.84 LLAGYY646 pKa = 10.27 RR647 pKa = 11.84 KK648 pKa = 9.2 MMEE651 pKa = 3.77 NALVHH656 pKa = 5.72 RR657 pKa = 11.84 VMMLDD662 pKa = 3.75 DD663 pKa = 4.64 GSFFTLEE670 pKa = 3.86 GMVPSGSLWTGICDD684 pKa = 3.35 TALNIMYY691 pKa = 8.2 ITSALRR697 pKa = 11.84 KK698 pKa = 9.97 LGFDD702 pKa = 4.65 DD703 pKa = 4.07 STFSPKK709 pKa = 10.26 CAGDD713 pKa = 4.26 DD714 pKa = 4.03 NLTCFQRR721 pKa = 11.84 RR722 pKa = 11.84 QGLDD726 pKa = 2.88 VMEE729 pKa = 4.78 RR730 pKa = 11.84 LRR732 pKa = 11.84 TTLNEE737 pKa = 3.85 MFRR740 pKa = 11.84 ANIDD744 pKa = 3.3 KK745 pKa = 10.77 KK746 pKa = 10.94 DD747 pKa = 4.53 FIVHH751 pKa = 5.56 YY752 pKa = 9.38 PPYY755 pKa = 10.94 AVTTVQACFEE765 pKa = 4.93 PGTDD769 pKa = 3.55 LSKK772 pKa = 10.44 GTSRR776 pKa = 11.84 MMDD779 pKa = 3.04 KK780 pKa = 11.24 AEE782 pKa = 3.99 WVPFTGPCPIDD793 pKa = 3.34 QAAGRR798 pKa = 11.84 SHH800 pKa = 6.25 RR801 pKa = 11.84 WKK803 pKa = 10.83 YY804 pKa = 11.29 VFTNKK809 pKa = 9.86 PKK811 pKa = 10.24 FLANFFLPDD820 pKa = 3.91 GKK822 pKa = 9.73 PIRR825 pKa = 11.84 PAHH828 pKa = 6.75 DD829 pKa = 3.76 NLEE832 pKa = 4.15 KK833 pKa = 10.93 LLWPEE838 pKa = 5.16 GIHH841 pKa = 6.89 EE842 pKa = 4.64 SIEE845 pKa = 4.25 DD846 pKa = 3.39 YY847 pKa = 10.22 MAAVLSMVVDD857 pKa = 3.74 NPFNHH862 pKa = 6.68 HH863 pKa = 5.94 NVNHH867 pKa = 5.5 MMHH870 pKa = 7.36 RR871 pKa = 11.84 YY872 pKa = 9.94 LIAQQIQKK880 pKa = 10.24 QSIFIDD886 pKa = 3.58 PAVVMEE892 pKa = 4.45 LAKK895 pKa = 10.01 TRR897 pKa = 11.84 PKK899 pKa = 10.47 HH900 pKa = 5.75 GEE902 pKa = 3.61 AVPFPEE908 pKa = 3.16 IAYY911 pKa = 9.24 YY912 pKa = 9.86 RR913 pKa = 11.84 KK914 pKa = 10.02 CEE916 pKa = 4.11 GYY918 pKa = 10.72 VDD920 pKa = 3.9 IEE922 pKa = 4.31 RR923 pKa = 11.84 EE924 pKa = 3.99 PEE926 pKa = 3.67 FQEE929 pKa = 4.12 FFKK932 pKa = 11.02 SFRR935 pKa = 11.84 EE936 pKa = 4.57 FISSVSTLYY945 pKa = 10.78 ARR947 pKa = 11.84 RR948 pKa = 11.84 SEE950 pKa = 4.13 GGIDD954 pKa = 2.83 AWRR957 pKa = 11.84 FMDD960 pKa = 5.13 IIRR963 pKa = 11.84 GQHH966 pKa = 6.21 SIGAGQFGNSVHH978 pKa = 5.32 EE979 pKa = 4.07 WCRR982 pKa = 11.84 FLGEE986 pKa = 4.36 HH987 pKa = 6.9 PLTRR991 pKa = 11.84 SLRR994 pKa = 11.84 KK995 pKa = 9.44 ARR997 pKa = 11.84 RR998 pKa = 11.84 FRR1000 pKa = 11.84 PMKK1003 pKa = 9.28 EE1004 pKa = 3.64 AVVADD1009 pKa = 4.24 PEE1011 pKa = 4.49 TLGKK1015 pKa = 9.95 VEE1017 pKa = 5.52 KK1018 pKa = 10.85 AFTWVLDD1025 pKa = 3.63 LCEE1028 pKa = 5.48 ANDD1031 pKa = 3.56 PMTPLFYY1038 pKa = 10.85 ASAVSDD1044 pKa = 3.36 ILSSNVSAPP1053 pKa = 3.23
Molecular weight: 118.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.429
IPC2_protein 7.834
IPC_protein 7.644
Toseland 7.38
ProMoST 8.287
Dawson 8.434
Bjellqvist 8.741
Wikipedia 8.361
Rodwell 8.478
Grimsley 7.424
Solomon 8.478
Lehninger 8.492
Nozaki 8.96
DTASelect 8.478
Thurlkill 8.565
EMBOSS 8.639
Sillero 8.873
Patrickios 4.698
IPC_peptide 8.478
IPC2_peptide 7.556
IPC2.peptide.svr19 7.791
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1438
385
1053
719.0
80.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.11 ± 0.887
1.599 ± 0.148
6.745 ± 0.644
5.981 ± 0.253
3.894 ± 0.639
6.05 ± 0.293
2.225 ± 0.055
4.242 ± 0.554
6.537 ± 0.619
10.083 ± 0.361
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.99 ± 0.45
3.06 ± 0.028
6.05 ± 0.602
4.659 ± 0.648
6.815 ± 0.414
5.981 ± 0.131
4.798 ± 0.451
5.633 ± 0.472
1.46 ± 0.336
2.086 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here