Avian metaavulavirus 20
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4NB37|A0A2Z4NB37_9MONO Isoform of A0A2I6ECF1 W protein OS=Avian metaavulavirus 20 OX=2560314 GN=P PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 5.31 FSNDD6 pKa = 3.27 DD7 pKa = 5.08 DD8 pKa = 3.8 IAEE11 pKa = 4.39 LLNVSSQVIRR21 pKa = 11.84 EE22 pKa = 4.03 IQHH25 pKa = 6.83 AEE27 pKa = 3.91 GKK29 pKa = 9.6 PPQTVGSVKK38 pKa = 10.17 VSPGNTKK45 pKa = 8.59 TLTEE49 pKa = 4.04 LWEE52 pKa = 4.5 SEE54 pKa = 3.99 ASQSQAAGPSDD65 pKa = 3.49 KK66 pKa = 11.26 SSGEE70 pKa = 4.08 SSNQPNPPQTNQTHH84 pKa = 5.64 NEE86 pKa = 3.86 EE87 pKa = 4.74 DD88 pKa = 3.74 SAEE91 pKa = 4.34 LDD93 pKa = 3.8 TITAATTTCNDD104 pKa = 3.71 NEE106 pKa = 4.49 DD107 pKa = 3.8 TTQQSSQGIKK117 pKa = 10.84 SNMGKK122 pKa = 9.99 DD123 pKa = 3.62 LDD125 pKa = 3.92 SALAKK130 pKa = 10.38 LEE132 pKa = 4.22 KK133 pKa = 10.02 KK134 pKa = 10.03 AASIKK139 pKa = 10.19 SDD141 pKa = 3.27 KK142 pKa = 10.54 QVLKK146 pKa = 10.76 GGEE149 pKa = 4.03 KK150 pKa = 10.2 RR151 pKa = 11.84 STANLGIPLFRR162 pKa = 11.84 EE163 pKa = 4.38 DD164 pKa = 3.28 QQ165 pKa = 3.53
Molecular weight: 17.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.719
IPC2_protein 4.558
IPC_protein 4.469
Toseland 4.317
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.304
Rodwell 4.304
Grimsley 4.215
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.698
Thurlkill 4.317
EMBOSS 4.317
Sillero 4.584
Patrickios 4.329
IPC_peptide 4.431
IPC2_peptide 4.571
IPC2.peptide.svr19 4.543
Protein with the highest isoelectric point:
>tr|A0A2I6ECF5|A0A2I6ECF5_9MONO Fusion glycoprotein F0 OS=Avian metaavulavirus 20 OX=2560314 GN=F PE=3 SV=2
MM1 pKa = 7.44 ATTTLQLYY9 pKa = 10.28 LDD11 pKa = 3.65 QADD14 pKa = 4.17 RR15 pKa = 11.84 GLEE18 pKa = 3.91 LLSFPLIVIQTSTGSKK34 pKa = 9.91 EE35 pKa = 4.11 LQQQMRR41 pKa = 11.84 IDD43 pKa = 3.93 NVGEE47 pKa = 3.87 TSGEE51 pKa = 3.83 KK52 pKa = 10.94 GEE54 pKa = 4.52 TIFINLYY61 pKa = 10.89 GFIKK65 pKa = 10.3 SQGVKK70 pKa = 10.01 EE71 pKa = 4.26 SSTEE75 pKa = 3.84 FLSVNSVQPKK85 pKa = 8.92 DD86 pKa = 3.52 TITAGMVVLGATRR99 pKa = 11.84 MSSDD103 pKa = 3.42 YY104 pKa = 11.25 SAIAADD110 pKa = 4.31 ALSLSITIKK119 pKa = 10.56 KK120 pKa = 9.74 SALAQEE126 pKa = 4.9 KK127 pKa = 10.08 IAVSIHH133 pKa = 5.74 NSPYY137 pKa = 10.26 SLSGALAVRR146 pKa = 11.84 SGGFIASAEE155 pKa = 4.09 EE156 pKa = 4.22 TIKK159 pKa = 10.9 CSSKK163 pKa = 9.88 IQAGVYY169 pKa = 7.24 YY170 pKa = 8.74 TFKK173 pKa = 10.84 PMFVSITRR181 pKa = 11.84 LNNGKK186 pKa = 10.26 LYY188 pKa = 10.15 RR189 pKa = 11.84 VPKK192 pKa = 10.46 AMHH195 pKa = 7.11 TINSDD200 pKa = 2.87 IFFKK204 pKa = 11.07 VQLEE208 pKa = 4.45 VEE210 pKa = 4.78 FKK212 pKa = 11.03 LGIKK216 pKa = 9.73 SDD218 pKa = 3.61 HH219 pKa = 6.73 PQAKK223 pKa = 8.67 MLKK226 pKa = 9.65 KK227 pKa = 10.61 VEE229 pKa = 3.99 IDD231 pKa = 3.42 GKK233 pKa = 9.8 PEE235 pKa = 3.61 YY236 pKa = 10.09 FGYY239 pKa = 10.58 CWFHH243 pKa = 6.12 LCNFKK248 pKa = 9.72 RR249 pKa = 11.84 TNARR253 pKa = 11.84 GEE255 pKa = 4.22 SRR257 pKa = 11.84 SLEE260 pKa = 4.3 AIKK263 pKa = 10.93 DD264 pKa = 3.73 KK265 pKa = 10.35 VRR267 pKa = 11.84 KK268 pKa = 8.38 MGLKK272 pKa = 10.04 ISIHH276 pKa = 6.03 DD277 pKa = 3.57 LWGPTIIVQITGKK290 pKa = 9.54 SSKK293 pKa = 9.55 YY294 pKa = 9.19 AQGFFSTRR302 pKa = 11.84 GTCCLPVSKK311 pKa = 10.77 SNPEE315 pKa = 3.72 LAKK318 pKa = 11.06 LMWSCDD324 pKa = 3.8 TKK326 pKa = 10.59 IHH328 pKa = 5.89 SALVIVQAGDD338 pKa = 3.01 KK339 pKa = 10.66 SKK341 pKa = 10.92 LIKK344 pKa = 10.67 SEE346 pKa = 4.63 DD347 pKa = 3.54 IEE349 pKa = 4.2 ISGAVVASSSGLPKK363 pKa = 10.76 YY364 pKa = 10.91 NLFKK368 pKa = 10.71 KK369 pKa = 10.36 GQVKK373 pKa = 10.32 GSKK376 pKa = 9.76
Molecular weight: 41.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.887
IPC2_protein 8.756
IPC_protein 8.609
Toseland 9.531
ProMoST 9.238
Dawson 9.75
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 10.233
Grimsley 9.794
Solomon 9.78
Lehninger 9.75
Nozaki 9.648
DTASelect 9.399
Thurlkill 9.619
EMBOSS 9.94
Sillero 9.706
Patrickios 5.715
IPC_peptide 9.78
IPC2_peptide 7.966
IPC2.peptide.svr19 7.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5047
165
2242
630.9
70.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.34 ± 0.946
2.12 ± 0.253
4.835 ± 0.306
5.251 ± 0.433
3.547 ± 0.538
5.33 ± 0.44
2.12 ± 0.342
7.291 ± 0.845
5.409 ± 0.469
10.462 ± 1.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.249
4.874 ± 0.336
3.943 ± 0.357
5.053 ± 0.402
4.181 ± 0.382
10.402 ± 0.636
6.638 ± 0.391
6.004 ± 0.275
1.129 ± 0.114
2.714 ± 0.326
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here