Geodermatophilus obscurus (strain ATCC 25078 / DSM 43160 / JCM 3152 / KCC A-0152 / KCTC 9177 / NBRC 13315 / NRRL B-3577 / G-20)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Geodermatophilus; Geodermatophilus obscurus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2S9Z7|D2S9Z7_GEOOG Fibronectin type III domain protein OS=Geodermatophilus obscurus (strain ATCC 25078 / DSM 43160 / JCM 3152 / KCC A-0152 / KCTC 9177 / NBRC 13315 / NRRL B-3577 / G-20) OX=526225 GN=Gobs_1101 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84LSKK5 pKa = 10.67RR6 pKa = 11.84SAALIATGLTGAMVLSACGGGGDD29 pKa = 3.92EE30 pKa = 5.6DD31 pKa = 4.45GGSGAAAADD40 pKa = 3.76GGSFTVYY47 pKa = 9.78IAEE50 pKa = 4.68PEE52 pKa = 4.08NPLIPGQTTEE62 pKa = 4.15TEE64 pKa = 4.57GAQVLYY70 pKa = 11.03SLFTGLVQYY79 pKa = 10.98DD80 pKa = 4.01SEE82 pKa = 4.64TNEE85 pKa = 3.64AVYY88 pKa = 9.94TGVADD93 pKa = 4.37SIEE96 pKa = 4.47SADD99 pKa = 3.52QTTWTVKK106 pKa = 10.78LKK108 pKa = 10.87DD109 pKa = 2.95GWTFHH114 pKa = 7.35DD115 pKa = 5.19GSPVRR120 pKa = 11.84AQSFVDD126 pKa = 3.4AWNWTAYY133 pKa = 10.39SPNAANSSYY142 pKa = 10.65FFANIAGYY150 pKa = 9.73DD151 pKa = 3.68QLQAPTDD158 pKa = 3.63DD159 pKa = 4.32AGNVTGDD166 pKa = 3.69PAATAMSGLRR176 pKa = 11.84VVDD179 pKa = 4.26DD180 pKa = 3.66LTFEE184 pKa = 4.44VTLSAPYY191 pKa = 9.76AQWPTTVGYY200 pKa = 10.17SAFYY204 pKa = 9.33PLPPAFFEE212 pKa = 4.69DD213 pKa = 3.42SAAFGEE219 pKa = 4.34QPIGNGPFRR228 pKa = 11.84ADD230 pKa = 3.26EE231 pKa = 4.33PFVPGTGVTLTRR243 pKa = 11.84YY244 pKa = 10.21DD245 pKa = 3.73EE246 pKa = 4.38YY247 pKa = 11.68GGDD250 pKa = 3.66KK251 pKa = 9.67PANAASVQYY260 pKa = 10.76VVYY263 pKa = 10.67AEE265 pKa = 3.97QATAYY270 pKa = 10.36RR271 pKa = 11.84DD272 pKa = 3.74LQAGNLDD279 pKa = 4.05IMDD282 pKa = 5.12EE283 pKa = 4.39LPPDD287 pKa = 3.82ALASAEE293 pKa = 4.2AEE295 pKa = 4.05LGDD298 pKa = 4.44RR299 pKa = 11.84LLQVEE304 pKa = 4.3QGDD307 pKa = 3.86ITSLGFPTYY316 pKa = 10.13DD317 pKa = 4.1EE318 pKa = 5.18RR319 pKa = 11.84FADD322 pKa = 3.82PNVRR326 pKa = 11.84RR327 pKa = 11.84AFSMAIDD334 pKa = 3.57RR335 pKa = 11.84EE336 pKa = 4.65SITEE340 pKa = 4.53AIFQGTRR347 pKa = 11.84IPATSFINPVVDD359 pKa = 4.82GYY361 pKa = 11.45RR362 pKa = 11.84EE363 pKa = 4.22GACDD367 pKa = 3.42VCEE370 pKa = 4.65LNVEE374 pKa = 4.19EE375 pKa = 5.2ANRR378 pKa = 11.84LLDD381 pKa = 3.33EE382 pKa = 5.09AGFDD386 pKa = 3.37RR387 pKa = 11.84SQPVDD392 pKa = 2.84LWFNAGAGHH401 pKa = 6.92EE402 pKa = 4.04IWMEE406 pKa = 3.69AVGNQIRR413 pKa = 11.84EE414 pKa = 4.31GLGVDD419 pKa = 3.46YY420 pKa = 10.1TLQGNLDD427 pKa = 3.56FAEE430 pKa = 4.18YY431 pKa = 10.76LPLQDD436 pKa = 4.0EE437 pKa = 4.51QGMTGPFRR445 pKa = 11.84SGWIMDD451 pKa = 3.65YY452 pKa = 10.74PVAEE456 pKa = 4.34NFLGPLYY463 pKa = 10.64SSTALPPGGSNVTFYY478 pKa = 11.58SNPEE482 pKa = 3.45FDD484 pKa = 5.86ALLQQGNSADD494 pKa = 3.83TNEE497 pKa = 4.42AAIQAYY503 pKa = 8.69QAAEE507 pKa = 4.03DD508 pKa = 3.9LLLRR512 pKa = 11.84DD513 pKa = 4.21MPATPLFYY521 pKa = 10.32RR522 pKa = 11.84VNQSAHH528 pKa = 5.97SEE530 pKa = 4.13NVDD533 pKa = 3.45NVVVDD538 pKa = 3.69AFNRR542 pKa = 11.84IDD544 pKa = 3.36TAAVQVVGG552 pKa = 4.19

Molecular weight: 59.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2SDM7|D2SDM7_GEOOG Uncharacterized protein OS=Geodermatophilus obscurus (strain ATCC 25078 / DSM 43160 / JCM 3152 / KCC A-0152 / KCTC 9177 / NBRC 13315 / NRRL B-3577 / G-20) OX=526225 GN=Gobs_1770 PE=4 SV=1
MM1 pKa = 7.24SRR3 pKa = 11.84RR4 pKa = 11.84HH5 pKa = 5.4RR6 pKa = 11.84VLRR9 pKa = 11.84SAIAAAAAAAAGLLTRR25 pKa = 11.84SMVRR29 pKa = 11.84GVRR32 pKa = 11.84RR33 pKa = 11.84PP34 pKa = 3.11

Molecular weight: 3.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4795

0

4795

1492083

30

2124

311.2

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.337 ± 0.053

0.695 ± 0.01

6.228 ± 0.027

5.591 ± 0.03

2.503 ± 0.02

9.642 ± 0.034

2.063 ± 0.016

2.572 ± 0.025

1.252 ± 0.022

10.751 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.543 ± 0.013

1.386 ± 0.015

6.395 ± 0.031

2.718 ± 0.019

8.492 ± 0.038

4.727 ± 0.023

5.987 ± 0.026

9.896 ± 0.04

1.483 ± 0.014

1.739 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski