Anaeromyxobacter sp. (strain Fw109-5)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Myxococcales; Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter; unclassified Anaeromyxobacter

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7HG71|A7HG71_ANADF Methyl-accepting chemotaxis sensory transducer OS=Anaeromyxobacter sp. (strain Fw109-5) OX=404589 GN=Anae109_3536 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84AAHH5 pKa = 6.35WLIAALATSALHH17 pKa = 6.42CGGGEE22 pKa = 4.13GRR24 pKa = 11.84SVADD28 pKa = 3.62PAIVDD33 pKa = 3.41VDD35 pKa = 3.6RR36 pKa = 11.84DD37 pKa = 4.07GVVSAEE43 pKa = 4.2DD44 pKa = 4.45CDD46 pKa = 5.84DD47 pKa = 3.75FDD49 pKa = 6.72ANVWRR54 pKa = 11.84RR55 pKa = 11.84VSAHH59 pKa = 6.83RR60 pKa = 11.84DD61 pKa = 2.76VDD63 pKa = 3.66RR64 pKa = 11.84DD65 pKa = 3.43GRR67 pKa = 11.84GVAGEE72 pKa = 4.43GVTCAGDD79 pKa = 3.54RR80 pKa = 11.84LPEE83 pKa = 4.13GWSADD88 pKa = 3.43GTDD91 pKa = 4.08CDD93 pKa = 5.72DD94 pKa = 3.48YY95 pKa = 11.74DD96 pKa = 3.64ARR98 pKa = 11.84RR99 pKa = 11.84WTMGEE104 pKa = 4.58GYY106 pKa = 9.58PDD108 pKa = 3.87ADD110 pKa = 3.41GDD112 pKa = 4.03GRR114 pKa = 11.84AGGALAPVCRR124 pKa = 11.84GDD126 pKa = 3.92ALPSGWADD134 pKa = 3.22VATDD138 pKa = 4.38CAPEE142 pKa = 4.45DD143 pKa = 3.55SSRR146 pKa = 11.84WGEE149 pKa = 3.94LPYY152 pKa = 10.75LYY154 pKa = 10.78VDD156 pKa = 3.7ADD158 pKa = 3.64GDD160 pKa = 4.08GFTTEE165 pKa = 4.21GVGVVCSGEE174 pKa = 4.22SLPPGYY180 pKa = 10.52AADD183 pKa = 5.06PSGQDD188 pKa = 4.33CDD190 pKa = 5.51DD191 pKa = 3.97GDD193 pKa = 3.81PRR195 pKa = 11.84AFAFTSAFHH204 pKa = 7.34DD205 pKa = 3.86GDD207 pKa = 3.7GDD209 pKa = 3.93GRR211 pKa = 11.84GGEE214 pKa = 4.15PGQVCAGDD222 pKa = 4.09HH223 pKa = 6.68LPAGWAAQGGDD234 pKa = 3.68CAEE237 pKa = 4.62GDD239 pKa = 3.77GQRR242 pKa = 11.84WQWLSYY248 pKa = 10.77SYY250 pKa = 11.45VDD252 pKa = 4.11RR253 pKa = 11.84DD254 pKa = 3.31YY255 pKa = 11.71DD256 pKa = 3.8GYY258 pKa = 11.34SVYY261 pKa = 10.59EE262 pKa = 4.19PGSLCGGGGLPSPYY276 pKa = 9.17STGPGWRR283 pKa = 11.84GNGDD287 pKa = 3.73CDD289 pKa = 3.65DD290 pKa = 3.95TDD292 pKa = 3.55VRR294 pKa = 11.84THH296 pKa = 5.76AVVYY300 pKa = 9.62GYY302 pKa = 11.24ADD304 pKa = 3.65SDD306 pKa = 3.35WDD308 pKa = 3.92RR309 pKa = 11.84VGGGALLTLCTAGSLPLGYY328 pKa = 10.33LEE330 pKa = 5.0TGGDD334 pKa = 4.25CAPDD338 pKa = 3.59DD339 pKa = 3.58ATRR342 pKa = 11.84WRR344 pKa = 11.84EE345 pKa = 3.56YY346 pKa = 10.63AYY348 pKa = 10.46SYY350 pKa = 11.16RR351 pKa = 11.84DD352 pKa = 3.09ADD354 pKa = 3.43GDD356 pKa = 3.87GRR358 pKa = 11.84FVYY361 pKa = 10.64QSGKK365 pKa = 9.2VCYY368 pKa = 9.57GAQLPPGYY376 pKa = 9.1ATSTSSYY383 pKa = 11.29DD384 pKa = 3.86CDD386 pKa = 5.24DD387 pKa = 3.95GDD389 pKa = 4.5ASIHH393 pKa = 5.35TEE395 pKa = 3.23LWGYY399 pKa = 11.17ADD401 pKa = 4.86EE402 pKa = 6.45DD403 pKa = 4.93DD404 pKa = 4.41DD405 pKa = 4.73TVGAGPAVRR414 pKa = 11.84YY415 pKa = 7.01CTAGALPADD424 pKa = 3.93RR425 pKa = 11.84VVTGTDD431 pKa = 3.48CAPTDD436 pKa = 3.41PAAWQKK442 pKa = 11.13LSYY445 pKa = 10.73AGLDD449 pKa = 3.41EE450 pKa = 6.27DD451 pKa = 5.37GDD453 pKa = 4.29GFTTRR458 pKa = 11.84VGGTLCVGAEE468 pKa = 4.03LPEE471 pKa = 4.5PYY473 pKa = 9.64RR474 pKa = 11.84ASAAGNDD481 pKa = 4.12CDD483 pKa = 5.79DD484 pKa = 5.14ADD486 pKa = 3.88TALWRR491 pKa = 11.84WTVLYY496 pKa = 9.97PDD498 pKa = 4.82ADD500 pKa = 3.76GDD502 pKa = 4.91GIGTPPRR509 pKa = 11.84EE510 pKa = 3.97IRR512 pKa = 11.84CLGEE516 pKa = 4.22TIPAGYY522 pKa = 9.97SLQGWDD528 pKa = 4.55EE529 pKa = 4.12QPADD533 pKa = 4.24PGAQAAADD541 pKa = 4.02GLDD544 pKa = 3.67EE545 pKa = 4.45ATSPP549 pKa = 4.36

Molecular weight: 57.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7HEJ8|A7HEJ8_ANADF Cyclic diguanosine monophosphate-binding protein OS=Anaeromyxobacter sp. (strain Fw109-5) OX=404589 GN=Anae109_2945 PE=4 SV=1
MM1 pKa = 7.83PAQKK5 pKa = 10.73GNRR8 pKa = 11.84SKK10 pKa = 11.05RR11 pKa = 11.84KK12 pKa = 7.67VANRR16 pKa = 11.84SKK18 pKa = 10.94LKK20 pKa = 9.84RR21 pKa = 11.84TTATRR26 pKa = 11.84KK27 pKa = 9.21RR28 pKa = 11.84ALSVKK33 pKa = 10.17RR34 pKa = 11.84KK35 pKa = 9.4RR36 pKa = 11.84KK37 pKa = 9.66AKK39 pKa = 9.92RR40 pKa = 11.84APKK43 pKa = 10.19

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4461

0

4461

1575732

38

12684

353.2

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.051 ± 0.086

0.921 ± 0.021

5.12 ± 0.035

6.48 ± 0.043

3.054 ± 0.022

9.159 ± 0.039

2.015 ± 0.023

3.132 ± 0.026

2.384 ± 0.037

10.76 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.587 ± 0.018

1.561 ± 0.02

6.135 ± 0.034

2.401 ± 0.019

9.412 ± 0.068

4.686 ± 0.039

4.577 ± 0.05

8.439 ± 0.039

1.263 ± 0.016

1.864 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski