Nanosalinarum sp. (strain J07AB56)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Nanohaloarchaea; Candidatus Nanosalinarum; unclassified Candidatus Nanosalinarum

Average proteome isoelectric point is 5.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0QCW2|G0QCW2_NANSJ Small-conductance mechanosensitive channel OS=Nanosalinarum sp. (strain J07AB56) OX=889962 GN=J07AB56_08380 PE=3 SV=1
DDD2 pKa = 6.29DD3 pKa = 4.72SDDD6 pKa = 3.94KK7 pKa = 11.04YY8 pKa = 10.54QGSFGSTTSPVPNSWDDD25 pKa = 3.33RR26 pKa = 11.84STGFLGLFGAQEEE39 pKa = 4.37YY40 pKa = 10.67DDD42 pKa = 4.33PRR44 pKa = 11.84GDDD47 pKa = 3.52DD48 pKa = 5.19GDDD51 pKa = 3.58DDD53 pKa = 5.97DD54 pKa = 5.53DD55 pKa = 5.65EEE57 pKa = 4.56TLDDD61 pKa = 3.58PTDDD65 pKa = 3.05GMAEEE70 pKa = 4.71DD71 pKa = 4.58GSSDDD76 pKa = 3.49SVSQIEEE83 pKa = 4.62KKK85 pKa = 9.7EEE87 pKa = 3.94GTPNANNNIRR97 pKa = 11.84IEEE100 pKa = 4.12EE101 pKa = 4.24EEE103 pKa = 4.3TKKK106 pKa = 10.32CDDD109 pKa = 3.29DDD111 pKa = 4.59GSCPSISSNFLPTYYY126 pKa = 9.84GGPNYYY132 pKa = 10.39IAQSNSDDD140 pKa = 3.55LKKK143 pKa = 10.03EE144 pKa = 3.64SKKK147 pKa = 9.69SRR149 pKa = 11.84GTTTLGSTTDDD160 pKa = 3.28YY161 pKa = 11.25DD162 pKa = 4.03EEE164 pKa = 4.18YY165 pKa = 10.84SYYY168 pKa = 11.25VVQSTGSATDDD179 pKa = 3.28SCTATGNYYY188 pKa = 9.37CNLDDD193 pKa = 3.3SNTVTTSKKK202 pKa = 9.95EEE204 pKa = 4.08SHHH207 pKa = 7.35DD208 pKa = 4.11YYY210 pKa = 11.33LQPDDD215 pKa = 4.17EE216 pKa = 5.5DD217 pKa = 4.09HHH219 pKa = 6.88ATTSDDD225 pKa = 4.91EEE227 pKa = 4.44TSNGVGYYY235 pKa = 10.43LNEEE239 pKa = 3.81TDDD242 pKa = 3.58LRR244 pKa = 11.84LEEE247 pKa = 4.36AHHH250 pKa = 7.55DD251 pKa = 3.72DD252 pKa = 4.97FDDD255 pKa = 3.49GNDDD259 pKa = 3.86IEEE262 pKa = 4.55EE263 pKa = 4.39PGGSTTIRR271 pKa = 11.84SYYY274 pKa = 11.32CSLDDD279 pKa = 3.31TPKKK283 pKa = 10.75DD284 pKa = 3.82SCDDD288 pKa = 3.18SNTGTYYY295 pKa = 7.16TYYY298 pKa = 11.0FTTSSPKKK306 pKa = 9.0TGTTSSQEEE315 pKa = 3.74FRR317 pKa = 11.84YYY319 pKa = 9.96EE320 pKa = 4.06VPSSGWPKKK329 pKa = 9.99TVLDDD334 pKa = 4.23NNDDD338 pKa = 2.98TGAVGEEE345 pKa = 4.51DD346 pKa = 4.06YY347 pKa = 10.03TVHHH351 pKa = 6.61HH352 pKa = 6.8KK353 pKa = 10.95KK354 pKa = 10.48EE355 pKa = 4.07GTGYYY360 pKa = 10.59SVDDD364 pKa = 3.31GRR366 pKa = 11.84DDD368 pKa = 3.19VDDD371 pKa = 3.75GNNRR375 pKa = 11.84AFGWSTFDDD384 pKa = 3.18TMDDD388 pKa = 3.67DDD390 pKa = 3.9EE391 pKa = 4.68ATEEE395 pKa = 4.18TGSEEE400 pKa = 4.05YYY402 pKa = 10.09EE403 pKa = 3.75DDD405 pKa = 3.57NNGEEE410 pKa = 4.29DDD412 pKa = 3.7LVAVHHH418 pKa = 6.47EE419 pKa = 4.54EEE421 pKa = 4.54VGDDD425 pKa = 3.68SNFGTYYY432 pKa = 10.52SRR434 pKa = 11.84SDDD437 pKa = 4.33SVSCPGDDD445 pKa = 3.35YY446 pKa = 8.99KKK448 pKa = 10.87VAAVDDD454 pKa = 3.95SLNSFD

Molecular weight: 48.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0QDK0|G0QDK0_NANSJ DNA-binding ferritin-like protein oxidative damage protectant OS=Nanosalinarum sp. (strain J07AB56) OX=889962 GN=J07AB56_10770 PE=3 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84QLKK5 pKa = 10.26LGSKK9 pKa = 9.4HH10 pKa = 5.87SRR12 pKa = 11.84RR13 pKa = 11.84LTHH16 pKa = 5.57QTPKK20 pKa = 9.28MKK22 pKa = 10.18TMRR25 pKa = 11.84TMSLPLSRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84SWKK39 pKa = 9.08VHH41 pKa = 4.18VRR43 pKa = 11.84HH44 pKa = 6.31

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1402

0

1402

319963

13

1377

228.2

25.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.615 ± 0.08

0.711 ± 0.02

7.512 ± 0.073

9.843 ± 0.115

3.515 ± 0.048

7.875 ± 0.076

1.803 ± 0.037

4.376 ± 0.051

3.695 ± 0.059

8.79 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.034

3.361 ± 0.051

3.869 ± 0.043

3.577 ± 0.048

6.368 ± 0.07

7.575 ± 0.077

5.561 ± 0.06

8.051 ± 0.076

0.854 ± 0.025

2.701 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski