Pleurocapsa sp. PCC 7327
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4255 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9TB87|K9TB87_9CYAN Ferredoxin (2Fe-2S) OS=Pleurocapsa sp. PCC 7327 OX=118163 GN=Ple7327_4593 PE=3 SV=1
MM1 pKa = 7.76 ANNNDD6 pKa = 4.2 IIQLTNNNFDD16 pKa = 4.11 DD17 pKa = 4.17 YY18 pKa = 11.72 GPRR21 pKa = 11.84 IDD23 pKa = 3.83 GNKK26 pKa = 9.32 VVWTGYY32 pKa = 10.01 PDD34 pKa = 4.33 FNDD37 pKa = 3.64 SEE39 pKa = 4.76 IYY41 pKa = 10.41 FYY43 pKa = 11.03 DD44 pKa = 3.92 GSKK47 pKa = 8.59 ITQLTNNNFYY57 pKa = 10.86 DD58 pKa = 3.89 YY59 pKa = 11.18 GPQISGNNVVWSGSDD74 pKa = 3.47 GNDD77 pKa = 3.5 DD78 pKa = 4.99 EE79 pKa = 6.4 IYY81 pKa = 10.57 LYY83 pKa = 10.84 DD84 pKa = 3.83 GNKK87 pKa = 6.51 TTRR90 pKa = 11.84 LTDD93 pKa = 3.3 NNLYY97 pKa = 10.54 DD98 pKa = 4.23 YY99 pKa = 11.17 SPQIDD104 pKa = 3.82 GNKK107 pKa = 8.47 VVWYY111 pKa = 9.0 AYY113 pKa = 9.66 PPDD116 pKa = 3.96 YY117 pKa = 10.62 SDD119 pKa = 4.1 SEE121 pKa = 4.38 IYY123 pKa = 10.4 FYY125 pKa = 11.12 DD126 pKa = 3.75 GSKK129 pKa = 9.97 IVQLTDD135 pKa = 3.37 NNFSDD140 pKa = 4.57 YY141 pKa = 11.22 SPQISGNKK149 pKa = 8.47 VVWSGYY155 pKa = 10.33 DD156 pKa = 3.37 GNDD159 pKa = 3.52 DD160 pKa = 4.95 EE161 pKa = 6.35 IYY163 pKa = 10.54 LYY165 pKa = 10.83 DD166 pKa = 3.86 GSKK169 pKa = 9.7 TIQLTNNNTYY179 pKa = 10.55 DD180 pKa = 3.75 YY181 pKa = 10.84 SPQIDD186 pKa = 3.65 GDD188 pKa = 4.02 KK189 pKa = 10.78 VVWSGYY195 pKa = 10.35 DD196 pKa = 3.37 GNDD199 pKa = 3.52 DD200 pKa = 4.95 EE201 pKa = 6.35 IYY203 pKa = 10.5 LYY205 pKa = 10.74 DD206 pKa = 3.72 GSKK209 pKa = 9.94 IVQLTDD215 pKa = 3.37 NNFSDD220 pKa = 4.57 YY221 pKa = 11.22 SPQISGNKK229 pKa = 8.41 VVWSGYY235 pKa = 9.67 PDD237 pKa = 4.01 FNDD240 pKa = 3.82 SEE242 pKa = 4.59 IYY244 pKa = 10.53 LYY246 pKa = 10.89 DD247 pKa = 3.91 GNQTIQLTDD256 pKa = 3.33 NNFYY260 pKa = 10.76 DD261 pKa = 4.2 YY262 pKa = 11.16 SPQISGDD269 pKa = 3.36 KK270 pKa = 9.7 VVWYY274 pKa = 8.7 GYY276 pKa = 9.48 PDD278 pKa = 3.52 NADD281 pKa = 3.57 SEE283 pKa = 4.91 IYY285 pKa = 10.37 FYY287 pKa = 11.3 DD288 pKa = 4.3 GDD290 pKa = 4.59 VIQLTNNNTYY300 pKa = 10.72 DD301 pKa = 4.0 YY302 pKa = 10.95 DD303 pKa = 3.95 PQISGNKK310 pKa = 8.44 VVWSSYY316 pKa = 11.06 DD317 pKa = 3.23 GTDD320 pKa = 3.16 RR321 pKa = 11.84 EE322 pKa = 4.34 IYY324 pKa = 10.19 LYY326 pKa = 9.25 EE327 pKa = 4.34 IPPGIEE333 pKa = 4.24 LIGTEE338 pKa = 4.31 GDD340 pKa = 3.81 DD341 pKa = 3.42 VLTGSRR347 pKa = 11.84 GPDD350 pKa = 3.4 TISGLGGNDD359 pKa = 3.18 VLQGLAGNDD368 pKa = 3.54 QILGGSGNDD377 pKa = 4.67 LITAGDD383 pKa = 4.0 GKK385 pKa = 10.74 DD386 pKa = 3.27 KK387 pKa = 11.74 AEE389 pKa = 4.47 GGSGNDD395 pKa = 3.65 TISGNGGNDD404 pKa = 2.78 ILIGNEE410 pKa = 3.61 GRR412 pKa = 11.84 DD413 pKa = 4.0 DD414 pKa = 3.68 ILGGEE419 pKa = 4.76 DD420 pKa = 3.69 NDD422 pKa = 5.63 SIDD425 pKa = 3.53 GGAGRR430 pKa = 11.84 DD431 pKa = 3.48 RR432 pKa = 11.84 LLGQAGNDD440 pKa = 3.83 TIVGQGGDD448 pKa = 3.67 DD449 pKa = 4.08 TIDD452 pKa = 3.83 GGDD455 pKa = 3.79 GTDD458 pKa = 3.59 SLSGNAGSDD467 pKa = 3.64 RR468 pKa = 11.84 ILGGSGSDD476 pKa = 3.4 SLSGGTEE483 pKa = 3.69 ADD485 pKa = 3.69 TLVGGFDD492 pKa = 3.84 NDD494 pKa = 4.13 SLDD497 pKa = 4.04 GGAGNDD503 pKa = 3.36 NLVGINLAVVSGAKK517 pKa = 10.03 VGFGAGEE524 pKa = 4.26 IDD526 pKa = 3.91 TLSGGTGYY534 pKa = 8.56 DD535 pKa = 3.34 TFALGDD541 pKa = 3.87 NDD543 pKa = 3.75 RR544 pKa = 11.84 VYY546 pKa = 11.48 YY547 pKa = 10.56 DD548 pKa = 5.07 DD549 pKa = 5.94 GDD551 pKa = 4.06 PLTTGEE557 pKa = 4.48 SDD559 pKa = 3.37 YY560 pKa = 11.7 TLITDD565 pKa = 4.29 YY566 pKa = 11.4 DD567 pKa = 3.96 PSQDD571 pKa = 3.12 SLQRR575 pKa = 11.84 KK576 pKa = 9.37 GSDD579 pKa = 3.18 DD580 pKa = 4.28 LYY582 pKa = 11.26 KK583 pKa = 10.44 LDD585 pKa = 4.43 YY586 pKa = 8.73 FTTPSGRR593 pKa = 11.84 VDD595 pKa = 2.89 VAMIYY600 pKa = 10.67 DD601 pKa = 4.03 PGVEE605 pKa = 4.14 ARR607 pKa = 11.84 GEE609 pKa = 4.27 TIAILQGSGASSASTASLSAQQTTDD634 pKa = 3.2 NTDD637 pKa = 3.06 EE638 pKa = 4.42 SPIPEE643 pKa = 4.24 NTSPLARR650 pKa = 11.84 LAGLAEE656 pKa = 4.42 GKK658 pKa = 10.2 DD659 pKa = 3.86 YY660 pKa = 11.5 ASGEE664 pKa = 4.41 LIVKK668 pKa = 9.57 LKK670 pKa = 10.97 AGTEE674 pKa = 4.01 ANKK677 pKa = 10.13 ISLLQEE683 pKa = 3.75 RR684 pKa = 11.84 LGATVLEE691 pKa = 4.52 TTKK694 pKa = 10.55 TLGIQRR700 pKa = 11.84 WKK702 pKa = 10.79 LEE704 pKa = 4.01 EE705 pKa = 4.28 GTSVRR710 pKa = 11.84 DD711 pKa = 4.19 AIASFSADD719 pKa = 3.3 PAIEE723 pKa = 4.3 YY724 pKa = 10.2 IEE726 pKa = 4.35 PNYY729 pKa = 9.97 IRR731 pKa = 11.84 STTATIPNDD740 pKa = 3.34 PRR742 pKa = 11.84 FSEE745 pKa = 4.41 LWGLNNTGQTGGTPDD760 pKa = 4.04 ADD762 pKa = 3.22 IDD764 pKa = 4.82 APEE767 pKa = 3.94 AWDD770 pKa = 3.77 RR771 pKa = 11.84 QTGSDD776 pKa = 3.76 VVVGVIDD783 pKa = 3.44 TGVDD787 pKa = 3.57 YY788 pKa = 11.17 NHH790 pKa = 7.81 PDD792 pKa = 3.46 LNDD795 pKa = 3.71 NMWTNPGEE803 pKa = 4.38 TPNNGVDD810 pKa = 3.78 DD811 pKa = 5.12 DD812 pKa = 5.07 GNGYY816 pKa = 9.87 VDD818 pKa = 5.48 DD819 pKa = 4.46 YY820 pKa = 11.47 YY821 pKa = 11.58 GYY823 pKa = 11.24 DD824 pKa = 3.58 FVNEE828 pKa = 4.7 DD829 pKa = 4.25 GDD831 pKa = 3.95 PFDD834 pKa = 5.8 DD835 pKa = 4.74 FFHH838 pKa = 6.28 GTHH841 pKa = 5.55 VAGTIAAEE849 pKa = 4.44 GNNNTGVTGVNWNAKK864 pKa = 8.84 IMAIKK869 pKa = 10.23 FLDD872 pKa = 3.84 SGGSGTDD879 pKa = 3.45 FDD881 pKa = 5.82 AIEE884 pKa = 4.08 AVEE887 pKa = 4.14 YY888 pKa = 8.63 STLMGVKK895 pKa = 8.92 LTSNSWGGYY904 pKa = 8.3 FFSQGLYY911 pKa = 10.56 DD912 pKa = 5.52 AIAAAGKK919 pKa = 10.13 AGQLFIAAAGNGTNDD934 pKa = 3.36 NDD936 pKa = 4.6 GPNPAYY942 pKa = 9.09 PASYY946 pKa = 10.81 DD947 pKa = 3.45 LDD949 pKa = 4.46 NIISVAATDD958 pKa = 3.95 ASDD961 pKa = 3.39 RR962 pKa = 11.84 LAWFSNYY969 pKa = 9.6 GATSVDD975 pKa = 3.35 LGAPGVSVLSTVPGGGYY992 pKa = 10.5 ASYY995 pKa = 10.79 DD996 pKa = 3.87 GTSMATPHH1004 pKa = 5.64 VSGVASLLWSEE1015 pKa = 4.97 DD1016 pKa = 3.68 PNLSAQEE1023 pKa = 4.12 VKK1025 pKa = 10.66 EE1026 pKa = 4.03 LLLTTVDD1033 pKa = 3.84 PLTVLEE1039 pKa = 4.65 GKK1041 pKa = 7.87 TVSGGRR1047 pKa = 11.84 LNAFNALSEE1056 pKa = 4.38 LGPPNEE1062 pKa = 4.61 IIGTEE1067 pKa = 4.06 GDD1069 pKa = 3.89 DD1070 pKa = 4.79 VITGTNRR1077 pKa = 11.84 RR1078 pKa = 11.84 DD1079 pKa = 3.64 RR1080 pKa = 11.84 ISGLGGNDD1088 pKa = 3.51 IIQALAGRR1096 pKa = 11.84 DD1097 pKa = 3.76 EE1098 pKa = 4.39 IFGGDD1103 pKa = 3.72 GDD1105 pKa = 4.84 DD1106 pKa = 6.49 LITADD1111 pKa = 4.18 EE1112 pKa = 4.76 GNDD1115 pKa = 3.67 TVEE1118 pKa = 4.5 GGAGRR1123 pKa = 11.84 DD1124 pKa = 3.21 RR1125 pKa = 11.84 IFGNDD1130 pKa = 3.43 GDD1132 pKa = 5.53 DD1133 pKa = 3.6 LLKK1136 pKa = 11.4 GNGGRR1141 pKa = 11.84 DD1142 pKa = 3.51 NILGGDD1148 pKa = 3.88 GNDD1151 pKa = 4.09 SIEE1154 pKa = 4.34 GGAGNDD1160 pKa = 3.24 RR1161 pKa = 11.84 LLGEE1165 pKa = 4.89 SGDD1168 pKa = 3.91 DD1169 pKa = 3.8 TILGNAGQDD1178 pKa = 3.38 TLDD1181 pKa = 3.78 GGEE1184 pKa = 4.91 GKK1186 pKa = 10.29 DD1187 pKa = 3.68 SLNGGTDD1194 pKa = 2.93 NDD1196 pKa = 5.24 LIFGGKK1202 pKa = 10.34 GEE1204 pKa = 4.46 DD1205 pKa = 3.67 SLVGEE1210 pKa = 5.13 AGADD1214 pKa = 3.61 SLTGGASNDD1223 pKa = 3.89 SLDD1226 pKa = 3.93 GGAGSDD1232 pKa = 3.4 RR1233 pKa = 11.84 LVGVDD1238 pKa = 3.91 PSASYY1243 pKa = 10.77 GAGEE1247 pKa = 4.65 LDD1249 pKa = 3.76 VLTGFSDD1256 pKa = 3.19 SDD1258 pKa = 3.85 TFVLGDD1264 pKa = 3.79 ASRR1267 pKa = 11.84 VFYY1270 pKa = 11.11 SDD1272 pKa = 4.28 GDD1274 pKa = 3.9 PLTTGEE1280 pKa = 4.45 SDD1282 pKa = 3.62 YY1283 pKa = 12.0 ALISDD1288 pKa = 4.97 FDD1290 pKa = 4.16 SSQDD1294 pKa = 3.23 IIQLHH1299 pKa = 5.7 GAIAQYY1305 pKa = 11.26 DD1306 pKa = 4.0 LDD1308 pKa = 4.48 FFTTSSGSINAALIYY1323 pKa = 10.67 DD1324 pKa = 4.38 PGDD1327 pKa = 3.33 AARR1330 pKa = 11.84 GEE1332 pKa = 4.73 LISILQNVPTDD1343 pKa = 4.12 LNLGSPAFSFVV1354 pKa = 2.94
Molecular weight: 142.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.35
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.567
Rodwell 3.414
Grimsley 3.249
Solomon 3.605
Lehninger 3.567
Nozaki 3.706
DTASelect 4.024
Thurlkill 3.414
EMBOSS 3.579
Sillero 3.719
Patrickios 1.329
IPC_peptide 3.605
IPC2_peptide 3.694
IPC2.peptide.svr19 3.677
Protein with the highest isoelectric point:
>tr|K9T3J3|K9T3J3_9CYAN Calcineurin-like phosphoesterase OS=Pleurocapsa sp. PCC 7327 OX=118163 GN=Ple7327_1620 PE=4 SV=1
MM1 pKa = 7.29 TRR3 pKa = 11.84 RR4 pKa = 11.84 TLEE7 pKa = 3.73 GTNRR11 pKa = 11.84 KK12 pKa = 7.62 QKK14 pKa = 8.83 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 9.55 TKK26 pKa = 10.27 NGRR29 pKa = 11.84 KK30 pKa = 8.82 VLQARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.48 GRR40 pKa = 11.84 HH41 pKa = 4.07 QLTVV45 pKa = 2.92
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4255
0
4255
1342602
29
2921
315.5
35.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.574 ± 0.036
1.036 ± 0.014
4.819 ± 0.036
6.589 ± 0.04
3.957 ± 0.032
6.778 ± 0.041
1.691 ± 0.015
6.811 ± 0.031
4.768 ± 0.037
11.212 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.865 ± 0.017
3.83 ± 0.031
4.744 ± 0.025
4.975 ± 0.041
5.817 ± 0.028
6.274 ± 0.03
5.266 ± 0.027
6.477 ± 0.031
1.487 ± 0.019
3.029 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here