Pleurocapsa sp. PCC 7327

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Pleurocapsales; Hyellaceae; Pleurocapsa; unclassified Pleurocapsa

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|K9TB87|K9TB87_9CYAN Ferredoxin (2Fe-2S) OS=Pleurocapsa sp. PCC 7327 OX=118163 GN=Ple7327_4593 PE=3 SV=1
MM1 pKa = 7.76ANNNDD6 pKa = 4.2IIQLTNNNFDD16 pKa = 4.11DD17 pKa = 4.17YY18 pKa = 11.72GPRR21 pKa = 11.84IDD23 pKa = 3.83GNKK26 pKa = 9.32VVWTGYY32 pKa = 10.01PDD34 pKa = 4.33FNDD37 pKa = 3.64SEE39 pKa = 4.76IYY41 pKa = 10.41FYY43 pKa = 11.03DD44 pKa = 3.92GSKK47 pKa = 8.59ITQLTNNNFYY57 pKa = 10.86DD58 pKa = 3.89YY59 pKa = 11.18GPQISGNNVVWSGSDD74 pKa = 3.47GNDD77 pKa = 3.5DD78 pKa = 4.99EE79 pKa = 6.4IYY81 pKa = 10.57LYY83 pKa = 10.84DD84 pKa = 3.83GNKK87 pKa = 6.51TTRR90 pKa = 11.84LTDD93 pKa = 3.3NNLYY97 pKa = 10.54DD98 pKa = 4.23YY99 pKa = 11.17SPQIDD104 pKa = 3.82GNKK107 pKa = 8.47VVWYY111 pKa = 9.0AYY113 pKa = 9.66PPDD116 pKa = 3.96YY117 pKa = 10.62SDD119 pKa = 4.1SEE121 pKa = 4.38IYY123 pKa = 10.4FYY125 pKa = 11.12DD126 pKa = 3.75GSKK129 pKa = 9.97IVQLTDD135 pKa = 3.37NNFSDD140 pKa = 4.57YY141 pKa = 11.22SPQISGNKK149 pKa = 8.47VVWSGYY155 pKa = 10.33DD156 pKa = 3.37GNDD159 pKa = 3.52DD160 pKa = 4.95EE161 pKa = 6.35IYY163 pKa = 10.54LYY165 pKa = 10.83DD166 pKa = 3.86GSKK169 pKa = 9.7TIQLTNNNTYY179 pKa = 10.55DD180 pKa = 3.75YY181 pKa = 10.84SPQIDD186 pKa = 3.65GDD188 pKa = 4.02KK189 pKa = 10.78VVWSGYY195 pKa = 10.35DD196 pKa = 3.37GNDD199 pKa = 3.52DD200 pKa = 4.95EE201 pKa = 6.35IYY203 pKa = 10.5LYY205 pKa = 10.74DD206 pKa = 3.72GSKK209 pKa = 9.94IVQLTDD215 pKa = 3.37NNFSDD220 pKa = 4.57YY221 pKa = 11.22SPQISGNKK229 pKa = 8.41VVWSGYY235 pKa = 9.67PDD237 pKa = 4.01FNDD240 pKa = 3.82SEE242 pKa = 4.59IYY244 pKa = 10.53LYY246 pKa = 10.89DD247 pKa = 3.91GNQTIQLTDD256 pKa = 3.33NNFYY260 pKa = 10.76DD261 pKa = 4.2YY262 pKa = 11.16SPQISGDD269 pKa = 3.36KK270 pKa = 9.7VVWYY274 pKa = 8.7GYY276 pKa = 9.48PDD278 pKa = 3.52NADD281 pKa = 3.57SEE283 pKa = 4.91IYY285 pKa = 10.37FYY287 pKa = 11.3DD288 pKa = 4.3GDD290 pKa = 4.59VIQLTNNNTYY300 pKa = 10.72DD301 pKa = 4.0YY302 pKa = 10.95DD303 pKa = 3.95PQISGNKK310 pKa = 8.44VVWSSYY316 pKa = 11.06DD317 pKa = 3.23GTDD320 pKa = 3.16RR321 pKa = 11.84EE322 pKa = 4.34IYY324 pKa = 10.19LYY326 pKa = 9.25EE327 pKa = 4.34IPPGIEE333 pKa = 4.24LIGTEE338 pKa = 4.31GDD340 pKa = 3.81DD341 pKa = 3.42VLTGSRR347 pKa = 11.84GPDD350 pKa = 3.4TISGLGGNDD359 pKa = 3.18VLQGLAGNDD368 pKa = 3.54QILGGSGNDD377 pKa = 4.67LITAGDD383 pKa = 4.0GKK385 pKa = 10.74DD386 pKa = 3.27KK387 pKa = 11.74AEE389 pKa = 4.47GGSGNDD395 pKa = 3.65TISGNGGNDD404 pKa = 2.78ILIGNEE410 pKa = 3.61GRR412 pKa = 11.84DD413 pKa = 4.0DD414 pKa = 3.68ILGGEE419 pKa = 4.76DD420 pKa = 3.69NDD422 pKa = 5.63SIDD425 pKa = 3.53GGAGRR430 pKa = 11.84DD431 pKa = 3.48RR432 pKa = 11.84LLGQAGNDD440 pKa = 3.83TIVGQGGDD448 pKa = 3.67DD449 pKa = 4.08TIDD452 pKa = 3.83GGDD455 pKa = 3.79GTDD458 pKa = 3.59SLSGNAGSDD467 pKa = 3.64RR468 pKa = 11.84ILGGSGSDD476 pKa = 3.4SLSGGTEE483 pKa = 3.69ADD485 pKa = 3.69TLVGGFDD492 pKa = 3.84NDD494 pKa = 4.13SLDD497 pKa = 4.04GGAGNDD503 pKa = 3.36NLVGINLAVVSGAKK517 pKa = 10.03VGFGAGEE524 pKa = 4.26IDD526 pKa = 3.91TLSGGTGYY534 pKa = 8.56DD535 pKa = 3.34TFALGDD541 pKa = 3.87NDD543 pKa = 3.75RR544 pKa = 11.84VYY546 pKa = 11.48YY547 pKa = 10.56DD548 pKa = 5.07DD549 pKa = 5.94GDD551 pKa = 4.06PLTTGEE557 pKa = 4.48SDD559 pKa = 3.37YY560 pKa = 11.7TLITDD565 pKa = 4.29YY566 pKa = 11.4DD567 pKa = 3.96PSQDD571 pKa = 3.12SLQRR575 pKa = 11.84KK576 pKa = 9.37GSDD579 pKa = 3.18DD580 pKa = 4.28LYY582 pKa = 11.26KK583 pKa = 10.44LDD585 pKa = 4.43YY586 pKa = 8.73FTTPSGRR593 pKa = 11.84VDD595 pKa = 2.89VAMIYY600 pKa = 10.67DD601 pKa = 4.03PGVEE605 pKa = 4.14ARR607 pKa = 11.84GEE609 pKa = 4.27TIAILQGSGASSASTASLSAQQTTDD634 pKa = 3.2NTDD637 pKa = 3.06EE638 pKa = 4.42SPIPEE643 pKa = 4.24NTSPLARR650 pKa = 11.84LAGLAEE656 pKa = 4.42GKK658 pKa = 10.2DD659 pKa = 3.86YY660 pKa = 11.5ASGEE664 pKa = 4.41LIVKK668 pKa = 9.57LKK670 pKa = 10.97AGTEE674 pKa = 4.01ANKK677 pKa = 10.13ISLLQEE683 pKa = 3.75RR684 pKa = 11.84LGATVLEE691 pKa = 4.52TTKK694 pKa = 10.55TLGIQRR700 pKa = 11.84WKK702 pKa = 10.79LEE704 pKa = 4.01EE705 pKa = 4.28GTSVRR710 pKa = 11.84DD711 pKa = 4.19AIASFSADD719 pKa = 3.3PAIEE723 pKa = 4.3YY724 pKa = 10.2IEE726 pKa = 4.35PNYY729 pKa = 9.97IRR731 pKa = 11.84STTATIPNDD740 pKa = 3.34PRR742 pKa = 11.84FSEE745 pKa = 4.41LWGLNNTGQTGGTPDD760 pKa = 4.04ADD762 pKa = 3.22IDD764 pKa = 4.82APEE767 pKa = 3.94AWDD770 pKa = 3.77RR771 pKa = 11.84QTGSDD776 pKa = 3.76VVVGVIDD783 pKa = 3.44TGVDD787 pKa = 3.57YY788 pKa = 11.17NHH790 pKa = 7.81PDD792 pKa = 3.46LNDD795 pKa = 3.71NMWTNPGEE803 pKa = 4.38TPNNGVDD810 pKa = 3.78DD811 pKa = 5.12DD812 pKa = 5.07GNGYY816 pKa = 9.87VDD818 pKa = 5.48DD819 pKa = 4.46YY820 pKa = 11.47YY821 pKa = 11.58GYY823 pKa = 11.24DD824 pKa = 3.58FVNEE828 pKa = 4.7DD829 pKa = 4.25GDD831 pKa = 3.95PFDD834 pKa = 5.8DD835 pKa = 4.74FFHH838 pKa = 6.28GTHH841 pKa = 5.55VAGTIAAEE849 pKa = 4.44GNNNTGVTGVNWNAKK864 pKa = 8.84IMAIKK869 pKa = 10.23FLDD872 pKa = 3.84SGGSGTDD879 pKa = 3.45FDD881 pKa = 5.82AIEE884 pKa = 4.08AVEE887 pKa = 4.14YY888 pKa = 8.63STLMGVKK895 pKa = 8.92LTSNSWGGYY904 pKa = 8.3FFSQGLYY911 pKa = 10.56DD912 pKa = 5.52AIAAAGKK919 pKa = 10.13AGQLFIAAAGNGTNDD934 pKa = 3.36NDD936 pKa = 4.6GPNPAYY942 pKa = 9.09PASYY946 pKa = 10.81DD947 pKa = 3.45LDD949 pKa = 4.46NIISVAATDD958 pKa = 3.95ASDD961 pKa = 3.39RR962 pKa = 11.84LAWFSNYY969 pKa = 9.6GATSVDD975 pKa = 3.35LGAPGVSVLSTVPGGGYY992 pKa = 10.5ASYY995 pKa = 10.79DD996 pKa = 3.87GTSMATPHH1004 pKa = 5.64VSGVASLLWSEE1015 pKa = 4.97DD1016 pKa = 3.68PNLSAQEE1023 pKa = 4.12VKK1025 pKa = 10.66EE1026 pKa = 4.03LLLTTVDD1033 pKa = 3.84PLTVLEE1039 pKa = 4.65GKK1041 pKa = 7.87TVSGGRR1047 pKa = 11.84LNAFNALSEE1056 pKa = 4.38LGPPNEE1062 pKa = 4.61IIGTEE1067 pKa = 4.06GDD1069 pKa = 3.89DD1070 pKa = 4.79VITGTNRR1077 pKa = 11.84RR1078 pKa = 11.84DD1079 pKa = 3.64RR1080 pKa = 11.84ISGLGGNDD1088 pKa = 3.51IIQALAGRR1096 pKa = 11.84DD1097 pKa = 3.76EE1098 pKa = 4.39IFGGDD1103 pKa = 3.72GDD1105 pKa = 4.84DD1106 pKa = 6.49LITADD1111 pKa = 4.18EE1112 pKa = 4.76GNDD1115 pKa = 3.67TVEE1118 pKa = 4.5GGAGRR1123 pKa = 11.84DD1124 pKa = 3.21RR1125 pKa = 11.84IFGNDD1130 pKa = 3.43GDD1132 pKa = 5.53DD1133 pKa = 3.6LLKK1136 pKa = 11.4GNGGRR1141 pKa = 11.84DD1142 pKa = 3.51NILGGDD1148 pKa = 3.88GNDD1151 pKa = 4.09SIEE1154 pKa = 4.34GGAGNDD1160 pKa = 3.24RR1161 pKa = 11.84LLGEE1165 pKa = 4.89SGDD1168 pKa = 3.91DD1169 pKa = 3.8TILGNAGQDD1178 pKa = 3.38TLDD1181 pKa = 3.78GGEE1184 pKa = 4.91GKK1186 pKa = 10.29DD1187 pKa = 3.68SLNGGTDD1194 pKa = 2.93NDD1196 pKa = 5.24LIFGGKK1202 pKa = 10.34GEE1204 pKa = 4.46DD1205 pKa = 3.67SLVGEE1210 pKa = 5.13AGADD1214 pKa = 3.61SLTGGASNDD1223 pKa = 3.89SLDD1226 pKa = 3.93GGAGSDD1232 pKa = 3.4RR1233 pKa = 11.84LVGVDD1238 pKa = 3.91PSASYY1243 pKa = 10.77GAGEE1247 pKa = 4.65LDD1249 pKa = 3.76VLTGFSDD1256 pKa = 3.19SDD1258 pKa = 3.85TFVLGDD1264 pKa = 3.79ASRR1267 pKa = 11.84VFYY1270 pKa = 11.11SDD1272 pKa = 4.28GDD1274 pKa = 3.9PLTTGEE1280 pKa = 4.45SDD1282 pKa = 3.62YY1283 pKa = 12.0ALISDD1288 pKa = 4.97FDD1290 pKa = 4.16SSQDD1294 pKa = 3.23IIQLHH1299 pKa = 5.7GAIAQYY1305 pKa = 11.26DD1306 pKa = 4.0LDD1308 pKa = 4.48FFTTSSGSINAALIYY1323 pKa = 10.67DD1324 pKa = 4.38PGDD1327 pKa = 3.33AARR1330 pKa = 11.84GEE1332 pKa = 4.73LISILQNVPTDD1343 pKa = 4.12LNLGSPAFSFVV1354 pKa = 2.94

Molecular weight:
142.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|K9T3J3|K9T3J3_9CYAN Calcineurin-like phosphoesterase OS=Pleurocapsa sp. PCC 7327 OX=118163 GN=Ple7327_1620 PE=4 SV=1
MM1 pKa = 7.29TRR3 pKa = 11.84RR4 pKa = 11.84TLEE7 pKa = 3.73GTNRR11 pKa = 11.84KK12 pKa = 7.62QKK14 pKa = 8.83RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MKK24 pKa = 9.55TKK26 pKa = 10.27NGRR29 pKa = 11.84KK30 pKa = 8.82VLQARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.48GRR40 pKa = 11.84HH41 pKa = 4.07QLTVV45 pKa = 2.92

Molecular weight:
5.4 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4255

0

4255

1342602

29

2921

315.5

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.574 ± 0.036

1.036 ± 0.014

4.819 ± 0.036

6.589 ± 0.04

3.957 ± 0.032

6.778 ± 0.041

1.691 ± 0.015

6.811 ± 0.031

4.768 ± 0.037

11.212 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.017

3.83 ± 0.031

4.744 ± 0.025

4.975 ± 0.041

5.817 ± 0.028

6.274 ± 0.03

5.266 ± 0.027

6.477 ± 0.031

1.487 ± 0.019

3.029 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski