Clostridium sp. CAG:1013

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2437 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5A2M0|R5A2M0_9CLOT DNA polymerase III alpha subunit Gram-positive type OS=Clostridium sp. CAG:1013 OX=1262769 GN=BN452_01052 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.28KK3 pKa = 9.88VLAMLLVFAMIASFAACGSSSNSSTVNEE31 pKa = 4.05EE32 pKa = 3.88SSAVSSDD39 pKa = 3.14KK40 pKa = 10.97VSSSTEE46 pKa = 4.08EE47 pKa = 3.84EE48 pKa = 4.4SSTGEE53 pKa = 4.13KK54 pKa = 10.52FSGSISIACSSQEE67 pKa = 3.64AKK69 pKa = 10.72ALQEE73 pKa = 3.95VLKK76 pKa = 10.72SYY78 pKa = 11.2NEE80 pKa = 3.91QEE82 pKa = 4.67DD83 pKa = 3.8VDD85 pKa = 4.62EE86 pKa = 4.34VTIDD90 pKa = 4.33PITVEE95 pKa = 4.09SVGDD99 pKa = 3.6FEE101 pKa = 6.39GLMTSWISSDD111 pKa = 4.07TLPDD115 pKa = 3.38MYY117 pKa = 10.45ISQVGSLQQSYY128 pKa = 10.09AANGYY133 pKa = 8.9LLPLTEE139 pKa = 4.52YY140 pKa = 11.07GFLDD144 pKa = 3.6RR145 pKa = 11.84LVAGDD150 pKa = 4.02TEE152 pKa = 4.83LIKK155 pKa = 10.9YY156 pKa = 10.46DD157 pKa = 3.7GDD159 pKa = 4.05DD160 pKa = 3.47YY161 pKa = 11.55AVPMNQSISVIYY173 pKa = 10.17VNNAALKK180 pKa = 9.29EE181 pKa = 3.99AGIEE185 pKa = 3.85IDD187 pKa = 3.33EE188 pKa = 4.45TNYY191 pKa = 10.3PNTWDD196 pKa = 3.79EE197 pKa = 4.57FVDD200 pKa = 4.49ILDD203 pKa = 3.65QLVAAGVQYY212 pKa = 10.4PFSLAGQDD220 pKa = 3.53ASAVTALPFQYY231 pKa = 9.71IYY233 pKa = 10.85QVVYY237 pKa = 10.01GANPNWYY244 pKa = 9.78ADD246 pKa = 3.58MLKK249 pKa = 10.74GEE251 pKa = 5.27NGAAWNDD258 pKa = 3.47EE259 pKa = 4.27LFLGMFDD266 pKa = 5.28KK267 pKa = 11.35YY268 pKa = 11.37GEE270 pKa = 4.01LLKK273 pKa = 11.01YY274 pKa = 10.63VSEE277 pKa = 4.56DD278 pKa = 3.29VLGVSKK284 pKa = 10.64DD285 pKa = 3.29EE286 pKa = 4.03RR287 pKa = 11.84LARR290 pKa = 11.84SVTGEE295 pKa = 3.79SPIYY299 pKa = 10.41VDD301 pKa = 3.96TSGAMPGIRR310 pKa = 11.84EE311 pKa = 4.66LDD313 pKa = 3.63PEE315 pKa = 5.75ADD317 pKa = 3.71VIMLPSCFVDD327 pKa = 5.34DD328 pKa = 5.22PADD331 pKa = 3.44QTIISSFDD339 pKa = 3.28SAVSITTGAEE349 pKa = 3.66KK350 pKa = 10.41KK351 pKa = 10.98GNVEE355 pKa = 4.06LCVDD359 pKa = 4.55FLDD362 pKa = 4.34YY363 pKa = 10.53LTTVEE368 pKa = 5.27GSTIFNNITGYY379 pKa = 10.7VPTIVDD385 pKa = 4.18CEE387 pKa = 4.69SEE389 pKa = 4.09VDD391 pKa = 3.55PAFDD395 pKa = 3.17IVLSILNKK403 pKa = 10.61GEE405 pKa = 4.37LPNSPMLSRR414 pKa = 11.84EE415 pKa = 4.27WIPGIKK421 pKa = 9.46EE422 pKa = 3.9VMKK425 pKa = 10.8NGCQNWLAGEE435 pKa = 4.35DD436 pKa = 3.93PKK438 pKa = 11.46SVADD442 pKa = 4.68TIQAEE447 pKa = 4.17QDD449 pKa = 3.4RR450 pKa = 11.84LEE452 pKa = 4.38EE453 pKa = 4.44ASPDD457 pKa = 3.27WVEE460 pKa = 5.76DD461 pKa = 3.71FLANYY466 pKa = 9.46NYY468 pKa = 10.59KK469 pKa = 10.65

Molecular weight: 51.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5A769|R5A769_9CLOT N-acetylmuramoyl-L-alanine amidase OS=Clostridium sp. CAG:1013 OX=1262769 GN=BN452_01609 PE=4 SV=1
MM1 pKa = 7.19IQSKK5 pKa = 10.18LGRR8 pKa = 11.84ILGVVLLLAGLMVLSACVGSYY29 pKa = 10.11PLSPEE34 pKa = 4.52DD35 pKa = 2.63IWRR38 pKa = 11.84ILLGQGGDD46 pKa = 3.32TMEE49 pKa = 5.66ARR51 pKa = 11.84VFWQLRR57 pKa = 11.84LPRR60 pKa = 11.84VLMGVAAGAVMSLSGSVYY78 pKa = 7.49QTIFRR83 pKa = 11.84NPLASPDD90 pKa = 3.73LTGVASGASLGAAGAIVLGTGGAMQLMLGAFMAGMLSVVLVLFLVRR136 pKa = 11.84FSRR139 pKa = 11.84FRR141 pKa = 11.84QMSSYY146 pKa = 9.74ILAGIVVSSVADD158 pKa = 3.67AGLMCLKK165 pKa = 10.67VMADD169 pKa = 3.69PEE171 pKa = 4.29RR172 pKa = 11.84EE173 pKa = 3.71LAAIEE178 pKa = 4.35FWTMGSLGAITAGKK192 pKa = 9.95LFPNLLVMAVPFVLLLLFHH211 pKa = 6.83RR212 pKa = 11.84QAVMLSLGDD221 pKa = 3.58EE222 pKa = 4.56QARR225 pKa = 11.84SMGLNPSLWRR235 pKa = 11.84GIFLVLSTLMIAGLVSVTGVISFVGLIGPHH265 pKa = 4.84IAFWIRR271 pKa = 11.84RR272 pKa = 11.84RR273 pKa = 11.84RR274 pKa = 11.84DD275 pKa = 2.72RR276 pKa = 11.84GYY278 pKa = 11.07FLLSSALGAVLVLTADD294 pKa = 3.44MLARR298 pKa = 11.84SLWKK302 pKa = 10.42GAEE305 pKa = 4.12LPLSIFTVFLAVPILILLLRR325 pKa = 11.84GRR327 pKa = 11.84KK328 pKa = 9.24GDD330 pKa = 3.62FHH332 pKa = 6.3GTAAA336 pKa = 4.43

Molecular weight: 35.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2437

0

2437

752331

29

2703

308.7

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.141 ± 0.05

1.637 ± 0.023

5.382 ± 0.041

7.303 ± 0.057

4.225 ± 0.038

7.872 ± 0.05

1.894 ± 0.027

5.506 ± 0.046

5.14 ± 0.045

10.107 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.025

3.404 ± 0.031

4.289 ± 0.037

3.92 ± 0.032

5.03 ± 0.049

5.809 ± 0.048

5.423 ± 0.043

7.446 ± 0.043

1.137 ± 0.021

3.579 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski