Amino acid dipepetide frequency for Clostridium sp. CAG:1013

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.402AlaAla: 8.402 ± 0.147	1.445AlaCys: 1.445 ± 0.045	4.066AlaAsp: 4.066 ± 0.082	4.838AlaGlu: 4.838 ± 0.079	3.464AlaPhe: 3.464 ± 0.074	6.585AlaGly: 6.585 ± 0.099	1.432AlaHis: 1.432 ± 0.053	4.349AlaIle: 4.349 ± 0.09	4.16AlaLys: 4.16 ± 0.077	9.109AlaLeu: 9.109 ± 0.132	2.516AlaMet: 2.516 ± 0.06	2.132AlaAsn: 2.132 ± 0.057	2.969AlaPro: 2.969 ± 0.071	3.958AlaGln: 3.958 ± 0.085	3.715AlaArg: 3.715 ± 0.069	4.337AlaSer: 4.337 ± 0.084	3.385AlaThr: 3.385 ± 0.069	7.057AlaVal: 7.057 ± 0.103	0.829AlaTrp: 0.829 ± 0.032	2.649AlaTyr: 2.649 ± 0.06	0.007AlaXaa: 0.007 ± 0.003
Cys	1.377CysAla: 1.377 ± 0.048	0.381CysCys: 0.381 ± 0.025	0.865CysAsp: 0.865 ± 0.038	0.869CysGlu: 0.869 ± 0.035	0.678CysPhe: 0.678 ± 0.033	1.87CysGly: 1.87 ± 0.059	0.368CysHis: 0.368 ± 0.022	0.748CysIle: 0.748 ± 0.029	0.714CysLys: 0.714 ± 0.035	1.455CysLeu: 1.455 ± 0.048	0.364CysMet: 0.364 ± 0.024	0.457CysAsn: 0.457 ± 0.028	0.807CysPro: 0.807 ± 0.033	0.677CysGln: 0.677 ± 0.028	0.966CysArg: 0.966 ± 0.032	0.976CysSer: 0.976 ± 0.04	0.782CysThr: 0.782 ± 0.028	1.285CysVal: 1.285 ± 0.04	0.194CysTrp: 0.194 ± 0.016	0.534CysTyr: 0.534 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	3.954AspAla: 3.954 ± 0.076	0.843AspCys: 0.843 ± 0.032	2.432AspAsp: 2.432 ± 0.067	3.437AspGlu: 3.437 ± 0.079	2.569AspPhe: 2.569 ± 0.063	4.635AspGly: 4.635 ± 0.099	1.012AspHis: 1.012 ± 0.043	3.09AspIle: 3.09 ± 0.068	2.568AspLys: 2.568 ± 0.071	5.522AspLeu: 5.522 ± 0.082	1.566AspMet: 1.566 ± 0.049	1.7AspAsn: 1.7 ± 0.057	2.882AspPro: 2.882 ± 0.061	2.09AspGln: 2.09 ± 0.053	2.714AspArg: 2.714 ± 0.067	2.983AspSer: 2.983 ± 0.074	3.082AspThr: 3.082 ± 0.062	3.77AspVal: 3.77 ± 0.07	0.735AspTrp: 0.735 ± 0.038	2.23AspTyr: 2.23 ± 0.051	0.003AspXaa: 0.003 ± 0.002
Glu	5.702GluAla: 5.702 ± 0.096	0.802GluCys: 0.802 ± 0.04	4.17GluAsp: 4.17 ± 0.081	7.719GluGlu: 7.719 ± 0.14	2.549GluPhe: 2.549 ± 0.065	5.339GluGly: 5.339 ± 0.094	1.305GluHis: 1.305 ± 0.046	4.123GluIle: 4.123 ± 0.084	4.999GluLys: 4.999 ± 0.091	6.756GluLeu: 6.756 ± 0.11	2.157GluMet: 2.157 ± 0.05	3.562GluAsn: 3.562 ± 0.076	2.367GluPro: 2.367 ± 0.062	2.854GluGln: 2.854 ± 0.074	3.654GluArg: 3.654 ± 0.082	3.492GluSer: 3.492 ± 0.081	4.053GluThr: 4.053 ± 0.075	4.212GluVal: 4.212 ± 0.093	0.637GluTrp: 0.637 ± 0.028	2.575GluTyr: 2.575 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	3.183PheAla: 3.183 ± 0.072	0.811PheCys: 0.811 ± 0.036	2.414PheAsp: 2.414 ± 0.066	2.309PheGlu: 2.309 ± 0.057	2.206PhePhe: 2.206 ± 0.072	3.455PheGly: 3.455 ± 0.079	0.981PheHis: 0.981 ± 0.037	2.016PheIle: 2.016 ± 0.061	1.324PheLys: 1.324 ± 0.036	4.606PheLeu: 4.606 ± 0.106	0.893PheMet: 0.893 ± 0.036	1.2PheAsn: 1.2 ± 0.041	1.878PhePro: 1.878 ± 0.046	1.93PheGln: 1.93 ± 0.056	2.116PheArg: 2.116 ± 0.05	3.221PheSer: 3.221 ± 0.07	2.604PheThr: 2.604 ± 0.059	2.898PheVal: 2.898 ± 0.067	0.54PheTrp: 0.54 ± 0.028	1.662PheTyr: 1.662 ± 0.054	0.003PheXaa: 0.003 ± 0.002
Gly	6.126GlyAla: 6.126 ± 0.121	1.45GlyCys: 1.45 ± 0.039	4.22GlyAsp: 4.22 ± 0.077	6.049GlyGlu: 6.049 ± 0.1	3.461GlyPhe: 3.461 ± 0.072	5.842GlyGly: 5.842 ± 0.109	1.469GlyHis: 1.469 ± 0.048	4.969GlyIle: 4.969 ± 0.094	5.133GlyLys: 5.133 ± 0.099	7.226GlyLeu: 7.226 ± 0.109	2.533GlyMet: 2.533 ± 0.056	2.68GlyAsn: 2.68 ± 0.061	2.099GlyPro: 2.099 ± 0.054	2.684GlyGln: 2.684 ± 0.065	3.576GlyArg: 3.576 ± 0.077	4.277GlySer: 4.277 ± 0.078	4.386GlyThr: 4.386 ± 0.088	6.576GlyVal: 6.576 ± 0.093	0.913GlyTrp: 0.913 ± 0.039	3.047GlyTyr: 3.047 ± 0.07	0.008GlyXaa: 0.008 ± 0.003
His	1.276HisAla: 1.276 ± 0.039	0.413HisCys: 0.413 ± 0.027	0.888HisAsp: 0.888 ± 0.04	0.828HisGlu: 0.828 ± 0.038	0.887HisPhe: 0.887 ± 0.034	1.697HisGly: 1.697 ± 0.055	0.55HisHis: 0.55 ± 0.034	1.158HisIle: 1.158 ± 0.036	0.787HisLys: 0.787 ± 0.034	1.768HisLeu: 1.768 ± 0.05	0.492HisMet: 0.492 ± 0.026	0.711HisAsn: 0.711 ± 0.032	1.142HisPro: 1.142 ± 0.041	0.772HisGln: 0.772 ± 0.035	1.085HisArg: 1.085 ± 0.043	1.069HisSer: 1.069 ± 0.041	1.058HisThr: 1.058 ± 0.039	1.312HisVal: 1.312 ± 0.045	0.282HisTrp: 0.282 ± 0.022	0.764HisTyr: 0.764 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	4.667IleAla: 4.667 ± 0.091	0.954IleCys: 0.954 ± 0.041	3.105IleAsp: 3.105 ± 0.065	2.855IleGlu: 2.855 ± 0.073	2.157IlePhe: 2.157 ± 0.076	3.889IleGly: 3.889 ± 0.083	1.162IleHis: 1.162 ± 0.049	3.104IleIle: 3.104 ± 0.09	2.242IleLys: 2.242 ± 0.055	5.98IleLeu: 5.98 ± 0.108	1.22IleMet: 1.22 ± 0.04	1.736IleAsn: 1.736 ± 0.052	3.028IlePro: 3.028 ± 0.06	2.369IleGln: 2.369 ± 0.051	3.074IleArg: 3.074 ± 0.059	3.819IleSer: 3.819 ± 0.083	3.455IleThr: 3.455 ± 0.079	3.859IleVal: 3.859 ± 0.083	0.533IleTrp: 0.533 ± 0.03	1.853IleTyr: 1.853 ± 0.047	0.001IleXaa: 0.001 ± 0.001
Lys	4.623LysAla: 4.623 ± 0.081	0.59LysCys: 0.59 ± 0.034	2.943LysAsp: 2.943 ± 0.072	4.753LysGlu: 4.753 ± 0.086	1.458LysPhe: 1.458 ± 0.045	3.647LysGly: 3.647 ± 0.075	0.772LysHis: 0.772 ± 0.032	2.991LysIle: 2.991 ± 0.06	3.618LysLys: 3.618 ± 0.082	4.775LysLeu: 4.775 ± 0.079	1.47LysMet: 1.47 ± 0.037	2.188LysAsn: 2.188 ± 0.052	1.999LysPro: 1.999 ± 0.046	1.711LysGln: 1.711 ± 0.056	2.805LysArg: 2.805 ± 0.066	2.446LysSer: 2.446 ± 0.056	2.976LysThr: 2.976 ± 0.059	3.395LysVal: 3.395 ± 0.079	0.48LysTrp: 0.48 ± 0.027	1.761LysTyr: 1.761 ± 0.049	0.001LysXaa: 0.001 ± 0.001
Leu	8.169LeuAla: 8.169 ± 0.113	2.104LeuCys: 2.104 ± 0.055	5.506LeuAsp: 5.506 ± 0.095	7.513LeuGlu: 7.513 ± 0.13	4.58LeuPhe: 4.58 ± 0.11	7.691LeuGly: 7.691 ± 0.12	1.862LeuHis: 1.862 ± 0.055	4.813LeuIle: 4.813 ± 0.107	4.821LeuLys: 4.821 ± 0.085	10.724LeuLeu: 10.724 ± 0.172	2.573LeuMet: 2.573 ± 0.061	3.116LeuAsn: 3.116 ± 0.071	5.054LeuPro: 5.054 ± 0.087	3.017LeuGln: 3.017 ± 0.063	5.409LeuArg: 5.409 ± 0.098	7.054LeuSer: 7.054 ± 0.109	5.632LeuThr: 5.632 ± 0.097	6.825LeuVal: 6.825 ± 0.109	1.159LeuTrp: 1.159 ± 0.043	3.447LeuTyr: 3.447 ± 0.073	0.003LeuXaa: 0.003 ± 0.002
Met	2.604MetAla: 2.604 ± 0.058	0.34MetCys: 0.34 ± 0.021	1.615MetAsp: 1.615 ± 0.048	2.503MetGlu: 2.503 ± 0.07	0.881MetPhe: 0.881 ± 0.034	2.249MetGly: 2.249 ± 0.061	0.366MetHis: 0.366 ± 0.027	1.535MetIle: 1.535 ± 0.049	1.828MetLys: 1.828 ± 0.045	2.495MetLeu: 2.495 ± 0.065	0.815MetMet: 0.815 ± 0.037	1.07MetAsn: 1.07 ± 0.036	1.187MetPro: 1.187 ± 0.041	0.723MetGln: 0.723 ± 0.027	1.215MetArg: 1.215 ± 0.04	1.473MetSer: 1.473 ± 0.04	1.513MetThr: 1.513 ± 0.043	2.095MetVal: 2.095 ± 0.057	0.213MetTrp: 0.213 ± 0.017	0.739MetTyr: 0.739 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	2.757AsnAla: 2.757 ± 0.058	0.562AsnCys: 0.562 ± 0.031	1.628AsnAsp: 1.628 ± 0.051	1.802AsnGlu: 1.802 ± 0.049	1.445AsnPhe: 1.445 ± 0.043	3.137AsnGly: 3.137 ± 0.071	0.669AsnHis: 0.669 ± 0.033	2.131AsnIle: 2.131 ± 0.063	1.412AsnLys: 1.412 ± 0.048	3.367AsnLeu: 3.367 ± 0.07	1.012AsnMet: 1.012 ± 0.034	1.107AsnAsn: 1.107 ± 0.038	1.838AsnPro: 1.838 ± 0.053	1.289AsnGln: 1.289 ± 0.043	1.761AsnArg: 1.761 ± 0.053	1.937AsnSer: 1.937 ± 0.055	1.873AsnThr: 1.873 ± 0.05	2.541AsnVal: 2.541 ± 0.066	0.445AsnTrp: 0.445 ± 0.027	1.331AsnTyr: 1.331 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.385ProAla: 3.385 ± 0.079	0.673ProCys: 0.673 ± 0.032	2.487ProAsp: 2.487 ± 0.063	4.24ProGlu: 4.24 ± 0.095	1.886ProPhe: 1.886 ± 0.055	3.401ProGly: 3.401 ± 0.075	0.848ProHis: 0.848 ± 0.036	2.031ProIle: 2.031 ± 0.057	1.962ProLys: 1.962 ± 0.054	4.018ProLeu: 4.018 ± 0.073	1.171ProMet: 1.171 ± 0.037	1.323ProAsn: 1.323 ± 0.043	1.328ProPro: 1.328 ± 0.057	2.177ProGln: 2.177 ± 0.061	1.656ProArg: 1.656 ± 0.049	2.386ProSer: 2.386 ± 0.055	2.187ProThr: 2.187 ± 0.066	3.693ProVal: 3.693 ± 0.071	0.548ProTrp: 0.548 ± 0.028	1.49ProTyr: 1.49 ± 0.041	0.004ProXaa: 0.004 ± 0.002
Gln	3.299GlnAla: 3.299 ± 0.076	0.483GlnCys: 0.483 ± 0.023	1.949GlnAsp: 1.949 ± 0.056	3.69GlnGlu: 3.69 ± 0.076	1.376GlnPhe: 1.376 ± 0.05	3.035GlnGly: 3.035 ± 0.078	0.578GlnHis: 0.578 ± 0.027	2.145GlnIle: 2.145 ± 0.061	2.307GlnLys: 2.307 ± 0.058	3.614GlnLeu: 3.614 ± 0.071	1.122GlnMet: 1.122 ± 0.038	1.547GlnAsn: 1.547 ± 0.045	1.495GlnPro: 1.495 ± 0.047	1.546GlnGln: 1.546 ± 0.052	2.151GlnArg: 2.151 ± 0.055	1.917GlnSer: 1.917 ± 0.053	1.869GlnThr: 1.869 ± 0.051	3.215GlnVal: 3.215 ± 0.06	0.502GlnTrp: 0.502 ± 0.028	1.365GlnTyr: 1.365 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	3.461ArgAla: 3.461 ± 0.069	0.795ArgCys: 0.795 ± 0.031	2.71ArgAsp: 2.71 ± 0.058	4.603ArgGlu: 4.603 ± 0.097	2.179ArgPhe: 2.179 ± 0.057	3.218ArgGly: 3.218 ± 0.067	1.008ArgHis: 1.008 ± 0.044	2.761ArgIle: 2.761 ± 0.068	2.964ArgLys: 2.964 ± 0.063	5.028ArgLeu: 5.028 ± 0.092	1.481ArgMet: 1.481 ± 0.048	1.695ArgAsn: 1.695 ± 0.046	2.015ArgPro: 2.015 ± 0.06	2.12ArgGln: 2.12 ± 0.059	3.207ArgArg: 3.207 ± 0.081	2.506ArgSer: 2.506 ± 0.066	2.378ArgThr: 2.378 ± 0.052	3.606ArgVal: 3.606 ± 0.062	0.651ArgTrp: 0.651 ± 0.03	1.913ArgTyr: 1.913 ± 0.053	0.003ArgXaa: 0.003 ± 0.002
Ser	4.503SerAla: 4.503 ± 0.091	0.812SerCys: 0.812 ± 0.036	2.827SerAsp: 2.827 ± 0.07	3.061SerGlu: 3.061 ± 0.071	2.75SerPhe: 2.75 ± 0.067	5.165SerGly: 5.165 ± 0.091	1.241SerHis: 1.241 ± 0.043	3.201SerIle: 3.201 ± 0.071	2.458SerLys: 2.458 ± 0.057	6.187SerLeu: 6.187 ± 0.094	1.62SerMet: 1.62 ± 0.045	1.724SerAsn: 1.724 ± 0.05	2.595SerPro: 2.595 ± 0.07	2.878SerGln: 2.878 ± 0.069	3.008SerArg: 3.008 ± 0.062	3.891SerSer: 3.891 ± 0.104	2.955SerThr: 2.955 ± 0.074	4.433SerVal: 4.433 ± 0.074	0.687SerTrp: 0.687 ± 0.031	2.09SerTyr: 2.09 ± 0.057	0.003SerXaa: 0.003 ± 0.002
Thr	4.798ThrAla: 4.798 ± 0.083	0.735ThrCys: 0.735 ± 0.029	2.829ThrAsp: 2.829 ± 0.063	3.247ThrGlu: 3.247 ± 0.068	2.25ThrPhe: 2.25 ± 0.058	4.939ThrGly: 4.939 ± 0.089	0.978ThrHis: 0.978 ± 0.035	3.194ThrIle: 3.194 ± 0.078	2.205ThrLys: 2.205 ± 0.063	5.807ThrLeu: 5.807 ± 0.087	1.35ThrMet: 1.35 ± 0.043	1.546ThrAsn: 1.546 ± 0.053	2.916ThrPro: 2.916 ± 0.08	2.066ThrGln: 2.066 ± 0.054	2.199ThrArg: 2.199 ± 0.056	2.997ThrSer: 2.997 ± 0.08	2.773ThrThr: 2.773 ± 0.074	4.89ThrVal: 4.89 ± 0.092	0.61ThrTrp: 0.61 ± 0.031	1.897ThrTyr: 1.897 ± 0.053	0.001ThrXaa: 0.001 ± 0.001
Val	5.569ValAla: 5.569 ± 0.088	1.416ValCys: 1.416 ± 0.041	4.146ValAsp: 4.146 ± 0.074	5.495ValGlu: 5.495 ± 0.096	3.314ValPhe: 3.314 ± 0.068	5.189ValGly: 5.189 ± 0.107	1.24ValHis: 1.24 ± 0.042	4.374ValIle: 4.374 ± 0.094	3.787ValLys: 3.787 ± 0.075	7.905ValLeu: 7.905 ± 0.158	2.003ValMet: 2.003 ± 0.054	2.576ValAsn: 2.576 ± 0.059	3.3ValPro: 3.3 ± 0.072	2.245ValGln: 2.245 ± 0.058	3.46ValArg: 3.46 ± 0.082	4.704ValSer: 4.704 ± 0.078	4.635ValThr: 4.635 ± 0.094	5.733ValVal: 5.733 ± 0.105	0.845ValTrp: 0.845 ± 0.036	2.525ValTyr: 2.525 ± 0.065	0.001ValXaa: 0.001 ± 0.002
Trp	0.879TrpAla: 0.879 ± 0.033	0.178TrpCys: 0.178 ± 0.014	0.755TrpAsp: 0.755 ± 0.036	0.998TrpGlu: 0.998 ± 0.044	0.471TrpPhe: 0.471 ± 0.022	0.916TrpGly: 0.916 ± 0.034	0.238TrpHis: 0.238 ± 0.019	0.528TrpIle: 0.528 ± 0.029	0.637TrpLys: 0.637 ± 0.028	1.051TrpLeu: 1.051 ± 0.039	0.379TrpMet: 0.379 ± 0.022	0.54TrpAsn: 0.54 ± 0.026	0.359TrpPro: 0.359 ± 0.022	0.461TrpGln: 0.461 ± 0.028	0.494TrpArg: 0.494 ± 0.029	0.57TrpSer: 0.57 ± 0.029	0.574TrpThr: 0.574 ± 0.026	0.759TrpVal: 0.759 ± 0.031	0.152TrpTrp: 0.152 ± 0.016	0.432TrpTyr: 0.432 ± 0.023	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.669TyrAla: 2.669 ± 0.067	0.582TyrCys: 0.582 ± 0.029	2.262TyrAsp: 2.262 ± 0.069	2.218TyrGlu: 2.218 ± 0.052	1.685TyrPhe: 1.685 ± 0.056	2.817TyrGly: 2.817 ± 0.059	0.857TyrHis: 0.857 ± 0.035	1.797TyrIle: 1.797 ± 0.051	1.475TyrLys: 1.475 ± 0.052	3.674TyrLeu: 3.674 ± 0.077	0.718TyrMet: 0.718 ± 0.032	1.434TyrAsn: 1.434 ± 0.053	1.628TyrPro: 1.628 ± 0.047	1.639TyrGln: 1.639 ± 0.051	2.032TyrArg: 2.032 ± 0.057	2.032TyrSer: 2.032 ± 0.055	2.059TyrThr: 2.059 ± 0.065	2.306TyrVal: 2.306 ± 0.054	0.413TyrTrp: 0.413 ± 0.026	1.486TyrTyr: 1.486 ± 0.051	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.007XaaGly: 0.007 ± 0.003	0.003XaaHis: 0.003 ± 0.002	0.004XaaIle: 0.004 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.007XaaPro: 0.007 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.004XaaArg: 0.004 ± 0.002	0.004XaaSer: 0.004 ± 0.002	0.001XaaThr: 0.001 ± 0.002	0.004XaaVal: 0.004 ± 0.002	0.003XaaTrp: 0.003 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.052XaaXaa: 0.052 ± 0.014

Statistics based on 2437 proteins (752331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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