Bordetella hinzii
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4557 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4VVG9|A0A0H4VVG9_9BORD Putative HTH-type transcriptional regulator YdcR OS=Bordetella hinzii OX=103855 GN=ydcR PE=3 SV=1
MM1 pKa = 7.58 SSTVNLVEE9 pKa = 4.17 LQPGAVLLMTDD20 pKa = 3.98 GAVVEE25 pKa = 5.11 VIEE28 pKa = 4.65 NPQDD32 pKa = 4.33 GIWITCRR39 pKa = 11.84 YY40 pKa = 8.99 LSHH43 pKa = 7.64 PGNPALVNGEE53 pKa = 4.11 EE54 pKa = 4.12 QQVFATDD61 pKa = 3.2 IQAYY65 pKa = 10.28 AEE67 pKa = 4.22 APP69 pKa = 3.46
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.834
IPC2_protein 3.91
IPC_protein 3.681
Toseland 3.541
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.541
Grimsley 3.478
Solomon 3.617
Lehninger 3.567
Nozaki 3.834
DTASelect 3.897
Thurlkill 3.617
EMBOSS 3.617
Sillero 3.808
Patrickios 0.477
IPC_peptide 3.617
IPC2_peptide 3.783
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A0H4WLZ6|A0A0H4WLZ6_9BORD ATP-dependent RNA helicase HrpB OS=Bordetella hinzii OX=103855 GN=hrpB PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.99 TRR25 pKa = 11.84 AGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4557
0
4557
1500318
29
10506
329.2
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.374 ± 0.053
0.862 ± 0.013
5.266 ± 0.027
5.063 ± 0.036
3.322 ± 0.027
8.738 ± 0.057
2.106 ± 0.018
4.422 ± 0.026
2.724 ± 0.036
11.169 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.021
2.428 ± 0.028
5.364 ± 0.033
4.035 ± 0.021
7.518 ± 0.041
5.114 ± 0.035
4.717 ± 0.033
7.464 ± 0.031
1.451 ± 0.018
2.417 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here