Amino acid dipepetide frequency for Rufibacter tibetensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.93AlaAla: 6.93 ± 0.095	0.653AlaCys: 0.653 ± 0.026	3.821AlaAsp: 3.821 ± 0.059	4.722AlaGlu: 4.722 ± 0.07	3.743AlaPhe: 3.743 ± 0.054	6.077AlaGly: 6.077 ± 0.081	1.472AlaHis: 1.472 ± 0.037	4.666AlaIle: 4.666 ± 0.062	4.358AlaLys: 4.358 ± 0.063	7.943AlaLeu: 7.943 ± 0.098	1.881AlaMet: 1.881 ± 0.037	3.398AlaAsn: 3.398 ± 0.065	3.196AlaPro: 3.196 ± 0.078	3.794AlaGln: 3.794 ± 0.057	3.261AlaArg: 3.261 ± 0.053	5.081AlaSer: 5.081 ± 0.086	4.562AlaThr: 4.562 ± 0.076	5.247AlaVal: 5.247 ± 0.068	0.927AlaTrp: 0.927 ± 0.03	2.822AlaTyr: 2.822 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.515CysAla: 0.515 ± 0.021	0.105CysCys: 0.105 ± 0.009	0.36CysAsp: 0.36 ± 0.019	0.407CysGlu: 0.407 ± 0.018	0.409CysPhe: 0.409 ± 0.017	0.641CysGly: 0.641 ± 0.035	0.204CysHis: 0.204 ± 0.014	0.442CysIle: 0.442 ± 0.02	0.328CysLys: 0.328 ± 0.014	0.811CysLeu: 0.811 ± 0.025	0.135CysMet: 0.135 ± 0.01	0.297CysAsn: 0.297 ± 0.014	0.33CysPro: 0.33 ± 0.018	0.313CysGln: 0.313 ± 0.014	0.338CysArg: 0.338 ± 0.019	0.535CysSer: 0.535 ± 0.02	0.458CysThr: 0.458 ± 0.028	0.465CysVal: 0.465 ± 0.019	0.074CysTrp: 0.074 ± 0.007	0.241CysTyr: 0.241 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.865AspAla: 3.865 ± 0.064	0.351AspCys: 0.351 ± 0.016	2.019AspAsp: 2.019 ± 0.046	3.012AspGlu: 3.012 ± 0.06	2.793AspPhe: 2.793 ± 0.045	3.389AspGly: 3.389 ± 0.067	0.953AspHis: 0.953 ± 0.03	3.083AspIle: 3.083 ± 0.052	3.024AspLys: 3.024 ± 0.05	5.189AspLeu: 5.189 ± 0.064	1.077AspMet: 1.077 ± 0.027	2.105AspAsn: 2.105 ± 0.04	2.284AspPro: 2.284 ± 0.044	1.996AspGln: 1.996 ± 0.04	2.29AspArg: 2.29 ± 0.046	2.626AspSer: 2.626 ± 0.047	2.461AspThr: 2.461 ± 0.046	3.417AspVal: 3.417 ± 0.053	0.757AspTrp: 0.757 ± 0.026	2.169AspTyr: 2.169 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.397GluAla: 5.397 ± 0.07	0.297GluCys: 0.297 ± 0.015	3.009GluAsp: 3.009 ± 0.05	5.099GluGlu: 5.099 ± 0.077	2.48GluPhe: 2.48 ± 0.046	4.153GluGly: 4.153 ± 0.059	1.255GluHis: 1.255 ± 0.033	3.786GluIle: 3.786 ± 0.058	4.478GluLys: 4.478 ± 0.069	5.988GluLeu: 5.988 ± 0.078	1.557GluMet: 1.557 ± 0.034	3.148GluAsn: 3.148 ± 0.049	2.07GluPro: 2.07 ± 0.038	3.085GluGln: 3.085 ± 0.054	3.158GluArg: 3.158 ± 0.046	2.943GluSer: 2.943 ± 0.045	3.26GluThr: 3.26 ± 0.049	4.956GluVal: 4.956 ± 0.066	0.748GluTrp: 0.748 ± 0.024	2.151GluTyr: 2.151 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.226PheAla: 3.226 ± 0.05	0.432PheCys: 0.432 ± 0.019	2.554PheAsp: 2.554 ± 0.039	2.642PheGlu: 2.642 ± 0.038	2.417PhePhe: 2.417 ± 0.052	3.341PheGly: 3.341 ± 0.05	0.956PheHis: 0.956 ± 0.023	2.598PheIle: 2.598 ± 0.049	2.656PheLys: 2.656 ± 0.045	4.908PheLeu: 4.908 ± 0.069	1.098PheMet: 1.098 ± 0.032	2.304PheAsn: 2.304 ± 0.043	1.885PhePro: 1.885 ± 0.039	1.905PheGln: 1.905 ± 0.048	2.083PheArg: 2.083 ± 0.043	3.614PheSer: 3.614 ± 0.057	3.1PheThr: 3.1 ± 0.063	2.938PheVal: 2.938 ± 0.051	0.665PheTrp: 0.665 ± 0.023	1.951PheTyr: 1.951 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	5.191GlyAla: 5.191 ± 0.069	0.649GlyCys: 0.649 ± 0.039	3.153GlyAsp: 3.153 ± 0.055	3.947GlyGlu: 3.947 ± 0.06	3.627GlyPhe: 3.627 ± 0.056	5.609GlyGly: 5.609 ± 0.09	1.432GlyHis: 1.432 ± 0.031	4.663GlyIle: 4.663 ± 0.066	5.047GlyLys: 5.047 ± 0.06	6.689GlyLeu: 6.689 ± 0.073	1.853GlyMet: 1.853 ± 0.04	3.44GlyAsn: 3.44 ± 0.056	2.102GlyPro: 2.102 ± 0.048	2.945GlyGln: 2.945 ± 0.049	3.171GlyArg: 3.171 ± 0.05	4.361GlySer: 4.361 ± 0.067	4.724GlyThr: 4.724 ± 0.1	5.052GlyVal: 5.052 ± 0.069	0.979GlyTrp: 0.979 ± 0.028	2.928GlyTyr: 2.928 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.344HisAla: 1.344 ± 0.034	0.193HisCys: 0.193 ± 0.012	0.94HisAsp: 0.94 ± 0.029	1.119HisGlu: 1.119 ± 0.029	1.132HisPhe: 1.132 ± 0.032	1.379HisGly: 1.379 ± 0.034	0.614HisHis: 0.614 ± 0.024	1.143HisIle: 1.143 ± 0.028	0.999HisLys: 0.999 ± 0.028	2.371HisLeu: 2.371 ± 0.051	0.411HisMet: 0.411 ± 0.019	0.864HisAsn: 0.864 ± 0.024	1.186HisPro: 1.186 ± 0.029	1.027HisGln: 1.027 ± 0.03	1.001HisArg: 1.001 ± 0.027	1.089HisSer: 1.089 ± 0.027	1.128HisThr: 1.128 ± 0.033	1.242HisVal: 1.242 ± 0.032	0.287HisTrp: 0.287 ± 0.014	0.914HisTyr: 0.914 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	4.602IleAla: 4.602 ± 0.06	0.548IleCys: 0.548 ± 0.018	3.224IleAsp: 3.224 ± 0.054	3.37IleGlu: 3.37 ± 0.058	2.438IlePhe: 2.438 ± 0.053	4.492IleGly: 4.492 ± 0.065	1.111IleHis: 1.111 ± 0.028	3.609IleIle: 3.609 ± 0.057	3.56IleLys: 3.56 ± 0.053	5.297IleLeu: 5.297 ± 0.071	1.209IleMet: 1.209 ± 0.032	2.824IleAsn: 2.824 ± 0.049	2.728IlePro: 2.728 ± 0.046	2.32IleGln: 2.32 ± 0.045	2.743IleArg: 2.743 ± 0.044	4.229IleSer: 4.229 ± 0.057	3.924IleThr: 3.924 ± 0.073	3.398IleVal: 3.398 ± 0.049	0.658IleTrp: 0.658 ± 0.023	2.003IleTyr: 2.003 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	5.07LysAla: 5.07 ± 0.07	0.264LysCys: 0.264 ± 0.013	3.346LysAsp: 3.346 ± 0.063	4.656LysGlu: 4.656 ± 0.062	2.065LysPhe: 2.065 ± 0.039	4.418LysGly: 4.418 ± 0.064	1.143LysHis: 1.143 ± 0.028	3.384LysIle: 3.384 ± 0.051	4.224LysLys: 4.224 ± 0.069	5.499LysLeu: 5.499 ± 0.069	1.58LysMet: 1.58 ± 0.04	3.042LysAsn: 3.042 ± 0.053	2.763LysPro: 2.763 ± 0.048	2.531LysGln: 2.531 ± 0.042	2.764LysArg: 2.764 ± 0.048	3.191LysSer: 3.191 ± 0.048	3.479LysThr: 3.479 ± 0.055	4.419LysVal: 4.419 ± 0.056	0.714LysTrp: 0.714 ± 0.022	2.146LysTyr: 2.146 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	7.722LeuAla: 7.722 ± 0.09	0.792LeuCys: 0.792 ± 0.028	4.855LeuAsp: 4.855 ± 0.079	6.288LeuGlu: 6.288 ± 0.081	4.615LeuPhe: 4.615 ± 0.074	6.47LeuGly: 6.47 ± 0.082	2.105LeuHis: 2.105 ± 0.045	5.199LeuIle: 5.199 ± 0.077	6.047LeuLys: 6.047 ± 0.07	10.579LeuLeu: 10.579 ± 0.145	2.242LeuMet: 2.242 ± 0.043	4.662LeuAsn: 4.662 ± 0.054	4.959LeuPro: 4.959 ± 0.063	4.902LeuGln: 4.902 ± 0.072	4.666LeuArg: 4.666 ± 0.064	6.566LeuSer: 6.566 ± 0.075	5.899LeuThr: 5.899 ± 0.068	6.729LeuVal: 6.729 ± 0.077	1.079LeuTrp: 1.079 ± 0.03	3.366LeuTyr: 3.366 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.085MetAla: 2.085 ± 0.041	0.117MetCys: 0.117 ± 0.009	1.142MetAsp: 1.142 ± 0.03	1.49MetGlu: 1.49 ± 0.03	0.827MetPhe: 0.827 ± 0.022	1.699MetGly: 1.699 ± 0.041	0.477MetHis: 0.477 ± 0.021	1.237MetIle: 1.237 ± 0.032	1.677MetLys: 1.677 ± 0.038	2.231MetLeu: 2.231 ± 0.041	0.615MetMet: 0.615 ± 0.024	1.143MetAsn: 1.143 ± 0.027	1.116MetPro: 1.116 ± 0.031	1.047MetGln: 1.047 ± 0.027	1.109MetArg: 1.109 ± 0.029	1.309MetSer: 1.309 ± 0.036	1.186MetThr: 1.186 ± 0.032	1.667MetVal: 1.667 ± 0.039	0.204MetTrp: 0.204 ± 0.012	0.664MetTyr: 0.664 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.498AsnAla: 3.498 ± 0.059	0.338AsnCys: 0.338 ± 0.016	2.056AsnAsp: 2.056 ± 0.035	2.513AsnGlu: 2.513 ± 0.045	2.255AsnPhe: 2.255 ± 0.05	3.705AsnGly: 3.705 ± 0.09	0.909AsnHis: 0.909 ± 0.026	2.884AsnIle: 2.884 ± 0.056	2.576AsnLys: 2.576 ± 0.046	4.697AsnLeu: 4.697 ± 0.063	1.044AsnMet: 1.044 ± 0.029	2.308AsnAsn: 2.308 ± 0.052	2.793AsnPro: 2.793 ± 0.05	2.144AsnGln: 2.144 ± 0.04	2.424AsnArg: 2.424 ± 0.043	2.824AsnSer: 2.824 ± 0.052	2.812AsnThr: 2.812 ± 0.05	3.046AsnVal: 3.046 ± 0.052	0.733AsnTrp: 0.733 ± 0.024	1.945AsnTyr: 1.945 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	3.943ProAla: 3.943 ± 0.073	0.235ProCys: 0.235 ± 0.014	2.529ProAsp: 2.529 ± 0.044	3.677ProGlu: 3.677 ± 0.057	2.151ProPhe: 2.151 ± 0.037	2.949ProGly: 2.949 ± 0.049	0.905ProHis: 0.905 ± 0.025	2.305ProIle: 2.305 ± 0.045	2.397ProLys: 2.397 ± 0.039	3.875ProLeu: 3.875 ± 0.062	0.879ProMet: 0.879 ± 0.025	2.05ProAsn: 2.05 ± 0.044	1.224ProPro: 1.224 ± 0.04	1.837ProGln: 1.837 ± 0.04	1.532ProArg: 1.532 ± 0.035	2.666ProSer: 2.666 ± 0.044	2.562ProThr: 2.562 ± 0.046	3.459ProVal: 3.459 ± 0.049	0.531ProTrp: 0.531 ± 0.022	1.657ProTyr: 1.657 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	3.622GlnAla: 3.622 ± 0.056	0.192GlnCys: 0.192 ± 0.013	2.077GlnAsp: 2.077 ± 0.04	3.399GlnGlu: 3.399 ± 0.051	1.793GlnPhe: 1.793 ± 0.036	2.69GlnGly: 2.69 ± 0.039	1.012GlnHis: 1.012 ± 0.029	2.396GlnIle: 2.396 ± 0.05	3.041GlnLys: 3.041 ± 0.043	4.456GlnLeu: 4.456 ± 0.071	1.071GlnMet: 1.071 ± 0.03	2.297GlnAsn: 2.297 ± 0.049	1.86GlnPro: 1.86 ± 0.038	2.323GlnGln: 2.323 ± 0.041	2.11GlnArg: 2.11 ± 0.043	2.077GlnSer: 2.077 ± 0.038	2.447GlnThr: 2.447 ± 0.044	3.267GlnVal: 3.267 ± 0.051	0.515GlnTrp: 0.515 ± 0.02	1.589GlnTyr: 1.589 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.016ArgAla: 3.016 ± 0.051	0.26ArgCys: 0.26 ± 0.015	2.121ArgAsp: 2.121 ± 0.043	3.06ArgGlu: 3.06 ± 0.053	2.364ArgPhe: 2.364 ± 0.044	2.733ArgGly: 2.733 ± 0.05	0.985ArgHis: 0.985 ± 0.032	3.009ArgIle: 3.009 ± 0.046	2.908ArgLys: 2.908 ± 0.054	4.746ArgLeu: 4.746 ± 0.07	1.276ArgMet: 1.276 ± 0.033	2.325ArgAsn: 2.325 ± 0.045	1.724ArgPro: 1.724 ± 0.034	2.135ArgGln: 2.135 ± 0.046	2.247ArgArg: 2.247 ± 0.043	2.46ArgSer: 2.46 ± 0.048	2.615ArgThr: 2.615 ± 0.049	3.113ArgVal: 3.113 ± 0.052	0.628ArgTrp: 0.628 ± 0.023	1.999ArgTyr: 1.999 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.607SerAla: 4.607 ± 0.072	0.568SerCys: 0.568 ± 0.023	2.748SerAsp: 2.748 ± 0.046	3.401SerGlu: 3.401 ± 0.048	3.529SerPhe: 3.529 ± 0.052	4.794SerGly: 4.794 ± 0.072	1.169SerHis: 1.169 ± 0.028	3.822SerIle: 3.822 ± 0.051	3.419SerLys: 3.419 ± 0.053	6.182SerLeu: 6.182 ± 0.065	1.386SerMet: 1.386 ± 0.034	2.789SerAsn: 2.789 ± 0.059	2.785SerPro: 2.785 ± 0.046	2.284SerGln: 2.284 ± 0.041	2.727SerArg: 2.727 ± 0.046	4.344SerSer: 4.344 ± 0.072	3.71SerThr: 3.71 ± 0.067	4.065SerVal: 4.065 ± 0.061	0.758SerTrp: 0.758 ± 0.026	2.409SerTyr: 2.409 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.705ThrAla: 4.705 ± 0.09	0.424ThrCys: 0.424 ± 0.029	2.934ThrAsp: 2.934 ± 0.044	3.143ThrGlu: 3.143 ± 0.053	2.914ThrPhe: 2.914 ± 0.067	4.822ThrGly: 4.822 ± 0.071	1.173ThrHis: 1.173 ± 0.03	3.655ThrIle: 3.655 ± 0.061	2.981ThrLys: 2.981 ± 0.047	5.908ThrLeu: 5.908 ± 0.065	1.071ThrMet: 1.071 ± 0.027	2.617ThrAsn: 2.617 ± 0.06	3.212ThrPro: 3.212 ± 0.045	2.31ThrGln: 2.31 ± 0.043	2.358ThrArg: 2.358 ± 0.047	3.886ThrSer: 3.886 ± 0.061	3.704ThrThr: 3.704 ± 0.075	4.278ThrVal: 4.278 ± 0.097	0.77ThrTrp: 0.77 ± 0.026	2.427ThrTyr: 2.427 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	5.489ValAla: 5.489 ± 0.076	0.581ValCys: 0.581 ± 0.024	3.328ValAsp: 3.328 ± 0.046	4.306ValGlu: 4.306 ± 0.052	3.16ValPhe: 3.16 ± 0.05	4.594ValGly: 4.594 ± 0.069	1.284ValHis: 1.284 ± 0.036	3.894ValIle: 3.894 ± 0.053	4.098ValLys: 4.098 ± 0.065	7.014ValLeu: 7.014 ± 0.083	1.566ValMet: 1.566 ± 0.031	3.276ValAsn: 3.276 ± 0.053	3.12ValPro: 3.12 ± 0.045	2.966ValGln: 2.966 ± 0.052	3.085ValArg: 3.085 ± 0.05	4.491ValSer: 4.491 ± 0.063	4.213ValThr: 4.213 ± 0.081	5.168ValVal: 5.168 ± 0.072	0.821ValTrp: 0.821 ± 0.029	2.548ValTyr: 2.548 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.922TrpAla: 0.922 ± 0.026	0.114TrpCys: 0.114 ± 0.009	0.634TrpAsp: 0.634 ± 0.023	0.786TrpGlu: 0.786 ± 0.028	0.557TrpPhe: 0.557 ± 0.023	0.868TrpGly: 0.868 ± 0.029	0.303TrpHis: 0.303 ± 0.015	0.628TrpIle: 0.628 ± 0.022	0.743TrpLys: 0.743 ± 0.027	1.323TrpLeu: 1.323 ± 0.036	0.361TrpMet: 0.361 ± 0.016	0.662TrpAsn: 0.662 ± 0.022	0.412TrpPro: 0.412 ± 0.016	0.629TrpGln: 0.629 ± 0.023	0.655TrpArg: 0.655 ± 0.024	0.735TrpSer: 0.735 ± 0.026	0.684TrpThr: 0.684 ± 0.026	0.898TrpVal: 0.898 ± 0.028	0.211TrpTrp: 0.211 ± 0.013	0.457TrpTyr: 0.457 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.808TyrAla: 2.808 ± 0.05	0.291TyrCys: 0.291 ± 0.014	2.012TyrAsp: 2.012 ± 0.04	1.983TyrGlu: 1.983 ± 0.038	2.004TyrPhe: 2.004 ± 0.048	2.738TyrGly: 2.738 ± 0.049	0.92TyrHis: 0.92 ± 0.028	1.882TyrIle: 1.882 ± 0.037	2.137TyrLys: 2.137 ± 0.04	4.037TyrLeu: 4.037 ± 0.058	0.728TyrMet: 0.728 ± 0.025	1.949TyrAsn: 1.949 ± 0.04	1.613TyrPro: 1.613 ± 0.039	1.743TyrGln: 1.743 ± 0.034	2.002TyrArg: 2.002 ± 0.045	2.471TyrSer: 2.471 ± 0.053	2.364TyrThr: 2.364 ± 0.064	2.18TyrVal: 2.18 ± 0.043	0.523TyrTrp: 0.523 ± 0.02	1.603TyrTyr: 1.603 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4108 proteins (1394976 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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