Tritonibacter horizontis
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4579 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A132BYW2|A0A132BYW2_9RHOB Galactofuranosyl transferase GlfT2 OS=Tritonibacter horizontis OX=1768241 GN=glfT2_1 PE=4 SV=1
MM1 pKa = 7.84 CEE3 pKa = 3.69 FCAATTVFDD12 pKa = 4.31 PARR15 pKa = 11.84 HH16 pKa = 5.9 NDD18 pKa = 3.6 SEE20 pKa = 4.2 GLRR23 pKa = 11.84 INGANFFEE31 pKa = 5.69 SGDD34 pKa = 3.6 ASDD37 pKa = 4.8 NLNTGYY43 pKa = 10.54 SLPVGGSFYY52 pKa = 11.17 GALDD56 pKa = 3.71 SVGDD60 pKa = 4.04 RR61 pKa = 11.84 DD62 pKa = 3.79 WVAVTLEE69 pKa = 4.32 AGVTYY74 pKa = 10.37 QISQSGYY81 pKa = 7.78 WGGGGTLYY89 pKa = 10.97 DD90 pKa = 3.62 GFLRR94 pKa = 11.84 VYY96 pKa = 10.36 NSNGQQVAGNDD107 pKa = 4.19 DD108 pKa = 3.47 GRR110 pKa = 11.84 HH111 pKa = 5.08 TYY113 pKa = 10.17 DD114 pKa = 3.84 AEE116 pKa = 4.36 VNMTVQSGGTFYY128 pKa = 10.71 ISVGSYY134 pKa = 10.1 RR135 pKa = 11.84 DD136 pKa = 3.72 SLTGTYY142 pKa = 10.52 RR143 pKa = 11.84 LDD145 pKa = 3.51 IDD147 pKa = 4.55 SNAPTVSATEE157 pKa = 3.87 EE158 pKa = 3.97 AGVQVLADD166 pKa = 4.03 YY167 pKa = 10.99 LVDD170 pKa = 4.86 GYY172 pKa = 10.21 WEE174 pKa = 3.82 EE175 pKa = 3.97 SGRR178 pKa = 11.84 DD179 pKa = 3.42 EE180 pKa = 5.44 RR181 pKa = 11.84 SFDD184 pKa = 3.41 VSEE187 pKa = 4.5 SNEE190 pKa = 3.58 ITVNLTGLTAAGQQLARR207 pKa = 11.84 WAFEE211 pKa = 3.59 AWEE214 pKa = 4.29 MVADD218 pKa = 4.22 LQFVEE223 pKa = 4.6 VSGAAQITYY232 pKa = 10.72 SDD234 pKa = 4.47 DD235 pKa = 3.43 SSGAYY240 pKa = 7.73 ATTRR244 pKa = 11.84 TWGTEE249 pKa = 3.34 ITGVDD254 pKa = 3.39 INISQDD260 pKa = 2.19 WLTRR264 pKa = 11.84 YY265 pKa = 8.02 GTEE268 pKa = 3.94 LNSYY272 pKa = 10.89 SMVAHH277 pKa = 5.05 VHH279 pKa = 5.4 EE280 pKa = 5.54 LGHH283 pKa = 6.66 ALGLGHH289 pKa = 6.44 QGYY292 pKa = 9.61 YY293 pKa = 10.47 NGGAVYY299 pKa = 10.38 GSNQNFVNDD308 pKa = 3.51 SWQMSVMSYY317 pKa = 10.04 FSQQEE322 pKa = 4.1 NTSVDD327 pKa = 3.07 ASYY330 pKa = 11.38 GLPITAMMADD340 pKa = 3.41 IVAIQSMYY348 pKa = 10.31 GAPGADD354 pKa = 3.28 AATAGDD360 pKa = 4.37 SLWGHH365 pKa = 6.39 GSDD368 pKa = 4.47 FGNYY372 pKa = 9.61 LDD374 pKa = 5.84 DD375 pKa = 4.22 VFDD378 pKa = 4.27 SFLLGGDD385 pKa = 3.37 AALGSNRR392 pKa = 11.84 NFVFTIYY399 pKa = 10.85 DD400 pKa = 3.32 SDD402 pKa = 4.76 GDD404 pKa = 3.98 DD405 pKa = 3.84 TLDD408 pKa = 4.02 LSSLSTRR415 pKa = 11.84 NTVDD419 pKa = 3.36 LNGGSFSDD427 pKa = 3.3 IQGRR431 pKa = 11.84 TGSLAIAEE439 pKa = 4.3 DD440 pKa = 4.19 TVIEE444 pKa = 3.97 NLIMGSGNDD453 pKa = 3.29 TVTGNEE459 pKa = 3.69 VDD461 pKa = 3.35 NHH463 pKa = 6.25 IEE465 pKa = 3.86 LGGGHH470 pKa = 6.96 DD471 pKa = 3.91 VAHH474 pKa = 6.92 GGAGTDD480 pKa = 4.13 RR481 pKa = 11.84 IWGGTGTDD489 pKa = 3.74 TIYY492 pKa = 11.13 GGDD495 pKa = 4.42 DD496 pKa = 3.27 RR497 pKa = 11.84 DD498 pKa = 3.44 ILYY501 pKa = 10.84 GNSSNDD507 pKa = 3.17 VLFGEE512 pKa = 5.14 GGNDD516 pKa = 3.52 LLVGQHH522 pKa = 6.89 HH523 pKa = 7.39 NDD525 pKa = 3.48 TLNGGLGNDD534 pKa = 3.88 VLSGGHH540 pKa = 6.15 GADD543 pKa = 3.48 VFCFDD548 pKa = 4.94 AGSGSDD554 pKa = 3.48 RR555 pKa = 11.84 IKK557 pKa = 10.92 DD558 pKa = 3.67 FKK560 pKa = 10.84 LWQGDD565 pKa = 3.95 SLQLDD570 pKa = 3.54 RR571 pKa = 11.84 DD572 pKa = 3.83 LVEE575 pKa = 6.2 DD576 pKa = 4.56 EE577 pKa = 5.04 LALDD581 pKa = 4.02 QLISTYY587 pKa = 11.03 GQVTDD592 pKa = 4.07 GGLVLTFDD600 pKa = 3.89 TGDD603 pKa = 3.31 TLEE606 pKa = 4.24 FMGIFDD612 pKa = 6.22 LDD614 pKa = 3.81 SADD617 pKa = 3.87 NDD619 pKa = 3.21 ILAWVV624 pKa = 3.82
Molecular weight: 66.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.503
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.139
Thurlkill 3.554
EMBOSS 3.706
Sillero 3.859
Patrickios 0.642
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A132BUE0|A0A132BUE0_9RHOB Aerobic cobaltochelatase subunit CobN OS=Tritonibacter horizontis OX=1768241 GN=cobN PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.3 LLDD5 pKa = 3.66 IAEE8 pKa = 4.38 VAQAAGVTPSTLRR21 pKa = 11.84 YY22 pKa = 9.21 YY23 pKa = 9.83 EE24 pKa = 4.28 EE25 pKa = 4.97 KK26 pKa = 11.17 GLIQPVARR34 pKa = 11.84 HH35 pKa = 5.12 GLRR38 pKa = 11.84 RR39 pKa = 11.84 QYY41 pKa = 11.0 GPQVLQQLSLVAMGKK56 pKa = 8.33 MAGFSLDD63 pKa = 2.9 EE64 pKa = 4.12 VAAMFGPTGRR74 pKa = 11.84 PDD76 pKa = 3.21 MPRR79 pKa = 11.84 NDD81 pKa = 3.88 LLRR84 pKa = 11.84 RR85 pKa = 11.84 AEE87 pKa = 4.03 RR88 pKa = 11.84 MQAQARR94 pKa = 11.84 QLDD97 pKa = 3.9 ALAQMLRR104 pKa = 11.84 HH105 pKa = 5.43 VAEE108 pKa = 5.13 CPAPTHH114 pKa = 6.62 LDD116 pKa = 3.22 CDD118 pKa = 3.81 KK119 pKa = 10.82 FQQLLRR125 pKa = 11.84 LATATQKK132 pKa = 10.16 RR133 pKa = 11.84 RR134 pKa = 11.84 RR135 pKa = 11.84 RR136 pKa = 11.84 SGQSPSRR143 pKa = 3.69
Molecular weight: 16.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.516
IPC_protein 10.365
Toseland 10.54
ProMoST 10.54
Dawson 10.657
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.774
Grimsley 10.701
Solomon 10.774
Lehninger 10.745
Nozaki 10.54
DTASelect 10.379
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.526
IPC_peptide 10.774
IPC2_peptide 9.487
IPC2.peptide.svr19 8.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4579
0
4579
1430001
29
2153
312.3
33.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.25 ± 0.045
0.927 ± 0.01
6.088 ± 0.032
5.805 ± 0.034
3.736 ± 0.022
8.436 ± 0.035
2.067 ± 0.018
5.079 ± 0.027
3.126 ± 0.03
10.306 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.73 ± 0.016
2.602 ± 0.021
4.992 ± 0.027
3.508 ± 0.021
6.677 ± 0.036
5.358 ± 0.022
5.546 ± 0.022
7.213 ± 0.029
1.344 ± 0.015
2.211 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here