Areca palm velarivirus 1
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G2UK88|A0A0G2UK88_9CLOS p18 protein OS=Areca palm velarivirus 1 OX=1654603 PE=4 SV=1
MM1 pKa = 7.2 LTTMEE6 pKa = 4.87 EE7 pKa = 3.93 LVDD10 pKa = 3.42 WMKK13 pKa = 11.07 SFEE16 pKa = 3.83 ISYY19 pKa = 10.41 EE20 pKa = 3.99 VFDD23 pKa = 4.2 MGEE26 pKa = 3.98 GSDD29 pKa = 4.01 FIIVLSAFDD38 pKa = 4.36 DD39 pKa = 4.04 GRR41 pKa = 11.84 EE42 pKa = 4.09 KK43 pKa = 10.16 IVYY46 pKa = 8.45 MSNNKK51 pKa = 9.23 YY52 pKa = 10.62 FSFKK56 pKa = 10.41 KK57 pKa = 9.92 IIYY60 pKa = 9.17 VRR62 pKa = 11.84 LPYY65 pKa = 10.21 LYY67 pKa = 10.47 SIFHH71 pKa = 7.4 RR72 pKa = 11.84 YY73 pKa = 8.18 LQVAPFYY80 pKa = 10.8 HH81 pKa = 6.75 RR82 pKa = 11.84 EE83 pKa = 3.79 WVNNPEE89 pKa = 4.69 SITVRR94 pKa = 11.84 WPEE97 pKa = 3.75 KK98 pKa = 9.89 QAVLEE103 pKa = 4.75 LLDD106 pKa = 4.06 EE107 pKa = 4.38 LQIHH111 pKa = 5.96 YY112 pKa = 9.29 EE113 pKa = 3.82 RR114 pKa = 11.84 SGPSNGKK121 pKa = 9.79 NILVLVRR128 pKa = 11.84 RR129 pKa = 11.84 TVDD132 pKa = 2.95 GFEE135 pKa = 4.44 TINYY139 pKa = 9.2 LSNNSNFDD147 pKa = 2.89 FDD149 pKa = 4.4 LFIRR153 pKa = 11.84 VDD155 pKa = 3.03 ISEE158 pKa = 4.1 QHH160 pKa = 5.66 SLIDD164 pKa = 3.29 IFLPSLPFHH173 pKa = 6.13 KK174 pKa = 10.75 CEE176 pKa = 3.99 WLL178 pKa = 3.61
Molecular weight: 21.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.884
IPC2_protein 4.978
IPC_protein 4.889
Toseland 4.813
ProMoST 5.016
Dawson 4.863
Bjellqvist 4.991
Wikipedia 4.724
Rodwell 4.774
Grimsley 4.724
Solomon 4.863
Lehninger 4.813
Nozaki 4.978
DTASelect 5.118
Thurlkill 4.8
EMBOSS 4.762
Sillero 5.054
Patrickios 3.745
IPC_peptide 4.863
IPC2_peptide 5.041
IPC2.peptide.svr19 5.025
Protein with the highest isoelectric point:
>tr|A0A0G2UG82|A0A0G2UG82_9CLOS RNA-dependent RNA polymerase (Fragment) OS=Areca palm velarivirus 1 OX=1654603 PE=4 SV=1
MM1 pKa = 7.54 EE2 pKa = 6.4 KK3 pKa = 10.01 ILISIFEE10 pKa = 4.39 CALMSDD16 pKa = 3.42 AVTIYY21 pKa = 8.88 RR22 pKa = 11.84 TNRR25 pKa = 11.84 SFLYY29 pKa = 10.58 NNYY32 pKa = 9.64 SQLSPVLLSKK42 pKa = 11.23 ANLIRR47 pKa = 11.84 YY48 pKa = 8.03 FYY50 pKa = 10.93 GQDD53 pKa = 2.71 AVAAIQAGLLAVRR66 pKa = 11.84 RR67 pKa = 11.84 HH68 pKa = 5.24 SRR70 pKa = 11.84 HH71 pKa = 5.87 LPSDD75 pKa = 3.31 LRR77 pKa = 11.84 ISLFSIILPDD87 pKa = 4.3 FNQKK91 pKa = 9.31 ILEE94 pKa = 4.13 FLKK97 pKa = 11.3 VMDD100 pKa = 4.17 QFLDD104 pKa = 4.0 TYY106 pKa = 10.65 HH107 pKa = 7.56 KK108 pKa = 10.72 LILIQLEE115 pKa = 4.05 IVIAEE120 pKa = 4.4 TKK122 pKa = 9.68 AQRR125 pKa = 11.84 NYY127 pKa = 11.16 DD128 pKa = 3.6 SGSNRR133 pKa = 11.84 IYY135 pKa = 11.14 GRR137 pKa = 11.84 IFGYY141 pKa = 9.99 SLNKK145 pKa = 9.91 GGSILKK151 pKa = 9.69 IWISFIAKK159 pKa = 9.72 SALAEE164 pKa = 4.11 QKK166 pKa = 11.0 SLII169 pKa = 4.17
Molecular weight: 19.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.849
IPC2_protein 9.121
IPC_protein 9.107
Toseland 9.516
ProMoST 9.385
Dawson 9.838
Bjellqvist 9.575
Wikipedia 10.058
Rodwell 10.043
Grimsley 9.94
Solomon 9.882
Lehninger 9.838
Nozaki 9.545
DTASelect 9.56
Thurlkill 9.648
EMBOSS 9.955
Sillero 9.75
Patrickios 4.762
IPC_peptide 9.867
IPC2_peptide 8.229
IPC2.peptide.svr19 7.979
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5284
36
2049
480.4
55.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.637 ± 0.562
1.911 ± 0.295
5.829 ± 0.33
4.939 ± 0.414
6.037 ± 0.473
3.671 ± 0.509
2.101 ± 0.142
8.081 ± 0.577
6.737 ± 0.321
10.522 ± 0.745
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.684 ± 0.204
6.586 ± 0.284
3.785 ± 0.369
3.653 ± 0.44
4.542 ± 0.274
7.702 ± 0.34
6.245 ± 0.541
6.132 ± 0.42
0.814 ± 0.135
4.391 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here