Eidolon helvum papillomavirus 2
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8YVV0|A0A1P8YVV0_9PAPI Replication protein E1 OS=Eidolon helvum papillomavirus 2 OX=1335476 GN=E1 PE=3 SV=1
MM1 pKa = 7.57 PAAKK5 pKa = 9.57 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 VRR10 pKa = 11.84 RR11 pKa = 11.84 EE12 pKa = 3.3 TVYY15 pKa = 10.73 RR16 pKa = 11.84 RR17 pKa = 11.84 VPRR20 pKa = 11.84 DD21 pKa = 3.14 TRR23 pKa = 11.84 SRR25 pKa = 11.84 RR26 pKa = 11.84 VIRR29 pKa = 11.84 DD30 pKa = 3.28 SVGNIYY36 pKa = 10.37 RR37 pKa = 11.84 NCKK40 pKa = 8.32 QWGTCPPDD48 pKa = 3.56 VINKK52 pKa = 9.1 VEE54 pKa = 4.23 QKK56 pKa = 9.6 TPADD60 pKa = 4.1 NILKK64 pKa = 9.41 YY65 pKa = 10.64 GSSAVFFGGLGIGTGRR81 pKa = 11.84 GSGGAGGYY89 pKa = 9.56 IPLGSGGGVRR99 pKa = 11.84 VGSWPWVPRR108 pKa = 11.84 YY109 pKa = 7.87 TGPTVEE115 pKa = 4.99 SIGPATIPDD124 pKa = 3.71 VTGSGWPWLPTYY136 pKa = 8.6 PTEE139 pKa = 4.21 AAGAGEE145 pKa = 4.4 TIEE148 pKa = 5.55 LEE150 pKa = 4.49 TILPTNTTVDD160 pKa = 3.84 EE161 pKa = 4.73 TPVVAPDD168 pKa = 3.4 APAVVDD174 pKa = 4.05 EE175 pKa = 4.57 LTNYY179 pKa = 7.45 PQQPTVIDD187 pKa = 3.59 STQPTTGGGRR197 pKa = 11.84 VIDD200 pKa = 4.05 VVAEE204 pKa = 3.88 VHH206 pKa = 6.56 HH207 pKa = 6.61 PVDD210 pKa = 4.59 FLPEE214 pKa = 3.93 HH215 pKa = 5.78 TLEE218 pKa = 4.63 SGPSVGSEE226 pKa = 3.67 PAVLDD231 pKa = 3.63 VGEE234 pKa = 5.18 EE235 pKa = 3.71 IPMFPRR241 pKa = 11.84 SRR243 pKa = 11.84 IHH245 pKa = 7.15 DD246 pKa = 3.84 HH247 pKa = 6.71 LEE249 pKa = 3.57 PSILHH254 pKa = 5.67 GTTSRR259 pKa = 11.84 GTSADD264 pKa = 3.09 VGGRR268 pKa = 11.84 FSIIDD273 pKa = 3.43 SSVAEE278 pKa = 4.12 TFIGEE283 pKa = 4.35 EE284 pKa = 4.64 PYY286 pKa = 9.4 STTPVRR292 pKa = 11.84 YY293 pKa = 9.81 DD294 pKa = 3.43 PEE296 pKa = 4.2 EE297 pKa = 3.95 PLQFRR302 pKa = 11.84 TSTPEE307 pKa = 3.68 TSRR310 pKa = 11.84 GPVARR315 pKa = 11.84 LKK317 pKa = 10.75 ALYY320 pKa = 10.24 NRR322 pKa = 11.84 FVQQIPVEE330 pKa = 4.41 DD331 pKa = 4.15 PLFVEE336 pKa = 4.7 APGGFVEE343 pKa = 4.67 YY344 pKa = 10.44 DD345 pKa = 3.21 NPAYY349 pKa = 10.64 DD350 pKa = 3.99 PDD352 pKa = 3.57 ATIEE356 pKa = 4.19 FNTEE360 pKa = 3.52 DD361 pKa = 3.67 ALEE364 pKa = 4.27 ATVAPDD370 pKa = 3.33 TRR372 pKa = 11.84 LSDD375 pKa = 3.55 LRR377 pKa = 11.84 VLHH380 pKa = 6.85 RR381 pKa = 11.84 PLLSEE386 pKa = 4.1 TPTGHH391 pKa = 5.84 VRR393 pKa = 11.84 VSRR396 pKa = 11.84 LGNFATMRR404 pKa = 11.84 LRR406 pKa = 11.84 SGAIPGPRR414 pKa = 11.84 AHH416 pKa = 6.88 VYY418 pKa = 10.54 HH419 pKa = 7.32 DD420 pKa = 4.17 LSSIGEE426 pKa = 4.33 SFEE429 pKa = 3.98 LTPFGSGRR437 pKa = 11.84 VSASTPVVLDD447 pKa = 3.55 TPVDD451 pKa = 3.97 VVVDD455 pKa = 3.84 TPVDD459 pKa = 3.6 VLTEE463 pKa = 4.08 DD464 pKa = 3.46 TTGLEE469 pKa = 4.72 DD470 pKa = 3.86 GLEE473 pKa = 4.14 DD474 pKa = 5.41 VPLLDD479 pKa = 4.48 HH480 pKa = 7.32 GSGEE484 pKa = 4.23 VQRR487 pKa = 11.84 VGVGRR492 pKa = 11.84 GGSTNRR498 pKa = 11.84 SFVSMEE504 pKa = 3.91 LPSTGRR510 pKa = 11.84 SDD512 pKa = 4.34 GFGTTISDD520 pKa = 3.56 IDD522 pKa = 3.54 SDD524 pKa = 4.78 AYY526 pKa = 10.31 VHH528 pKa = 6.35 YY529 pKa = 8.42 PTDD532 pKa = 3.32 STEE535 pKa = 3.84 PVAPSFGPGIPVEE548 pKa = 4.07 PAGPSIDD555 pKa = 3.79 VGDD558 pKa = 3.72 VGIDD562 pKa = 3.66 YY563 pKa = 10.9 FLDD566 pKa = 4.57 PYY568 pKa = 10.99 LFRR571 pKa = 11.84 KK572 pKa = 8.7 RR573 pKa = 11.84 RR574 pKa = 11.84 KK575 pKa = 8.51 RR576 pKa = 11.84 KK577 pKa = 9.63 RR578 pKa = 11.84 FFSFADD584 pKa = 3.46 DD585 pKa = 4.21 HH586 pKa = 7.01 VDD588 pKa = 3.18 SS589 pKa = 4.89
Molecular weight: 63.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.806
IPC2_protein 4.774
IPC_protein 4.736
Toseland 4.609
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.584
Rodwell 4.596
Grimsley 4.52
Solomon 4.711
Lehninger 4.66
Nozaki 4.813
DTASelect 5.003
Thurlkill 4.609
EMBOSS 4.609
Sillero 4.876
Patrickios 4.062
IPC_peptide 4.711
IPC2_peptide 4.876
IPC2.peptide.svr19 4.807
Protein with the highest isoelectric point:
>tr|A0A1P8YVV8|A0A1P8YVV8_9PAPI Protein E6 OS=Eidolon helvum papillomavirus 2 OX=1335476 GN=E6 PE=3 SV=1
MM1 pKa = 7.38 LTTFFIFRR9 pKa = 11.84 EE10 pKa = 4.45 SKK12 pKa = 9.68 TDD14 pKa = 3.4 TGCGRR19 pKa = 11.84 SIKK22 pKa = 10.64 GPLRR26 pKa = 11.84 LPFAPMAHH34 pKa = 7.07 PEE36 pKa = 4.41 SITDD40 pKa = 3.98 LARR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 GTTVRR50 pKa = 11.84 DD51 pKa = 3.26 ITIPCGFCNEE61 pKa = 4.53 PLNDD65 pKa = 3.42 QDD67 pKa = 5.56 KK68 pKa = 10.71 YY69 pKa = 11.49 AFDD72 pKa = 3.95 VRR74 pKa = 11.84 NHH76 pKa = 6.43 RR77 pKa = 11.84 LLNRR81 pKa = 11.84 RR82 pKa = 11.84 GVYY85 pKa = 9.97 FGCCTPCVRR94 pKa = 11.84 ALARR98 pKa = 11.84 KK99 pKa = 9.89 DD100 pKa = 3.45 RR101 pKa = 11.84 DD102 pKa = 3.74 TGNLAPFTVAEE113 pKa = 4.17 AEE115 pKa = 4.26 AYY117 pKa = 9.09 VGKK120 pKa = 10.32 NIGQILVLCSCCLKK134 pKa = 10.76 RR135 pKa = 11.84 LDD137 pKa = 5.2 LIEE140 pKa = 4.63 KK141 pKa = 9.91 LIHH144 pKa = 5.49 QRR146 pKa = 11.84 AGFYY150 pKa = 10.75 LCMVRR155 pKa = 11.84 DD156 pKa = 3.9 GEE158 pKa = 4.25 FRR160 pKa = 11.84 GICRR164 pKa = 11.84 LCMVLEE170 pKa = 4.39 GQEE173 pKa = 3.84
Molecular weight: 19.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.816
IPC2_protein 7.849
IPC_protein 7.658
Toseland 7.088
ProMoST 8.244
Dawson 8.346
Bjellqvist 8.887
Wikipedia 8.244
Rodwell 8.361
Grimsley 7.044
Solomon 8.419
Lehninger 8.448
Nozaki 9.253
DTASelect 8.492
Thurlkill 8.551
EMBOSS 8.551
Sillero 8.946
Patrickios 4.291
IPC_peptide 8.419
IPC2_peptide 8.434
IPC2.peptide.svr19 8.525
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2439
166
663
406.5
45.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.56 ± 0.981
2.132 ± 0.633
6.191 ± 0.476
6.642 ± 0.402
3.526 ± 0.225
7.708 ± 0.916
2.009 ± 0.165
4.059 ± 0.296
3.649 ± 0.745
7.831 ± 0.591
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.517
3.198 ± 0.732
6.765 ± 1.139
4.223 ± 0.896
7.339 ± 0.926
6.56 ± 0.573
7.503 ± 0.516
7.298 ± 0.934
1.312 ± 0.262
3.403 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here