Sanxia water strider virus 19
Average proteome isoelectric point is 8.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHR7|A0A1L3KHR7_9VIRU Uncharacterized protein OS=Sanxia water strider virus 19 OX=1923403 PE=4 SV=1
MM1 pKa = 7.51 APIPTHH7 pKa = 6.7 LLTPFSDD14 pKa = 2.92 NHH16 pKa = 6.41 LAFQTSIMDD25 pKa = 3.95 ASNPVLDD32 pKa = 4.29 SSRR35 pKa = 11.84 MTIRR39 pKa = 11.84 SMMEE43 pKa = 2.99 KK44 pKa = 9.42 HH45 pKa = 6.7 ARR47 pKa = 11.84 RR48 pKa = 11.84 QRR50 pKa = 11.84 QEE52 pKa = 3.36 LSYY55 pKa = 10.35 VARR58 pKa = 11.84 LAEE61 pKa = 4.31 EE62 pKa = 4.23 YY63 pKa = 10.37 PRR65 pKa = 11.84 VRR67 pKa = 11.84 FGKK70 pKa = 10.42 GVAKK74 pKa = 10.33 EE75 pKa = 3.88 MSRR78 pKa = 11.84 EE79 pKa = 4.03 DD80 pKa = 4.62 KK81 pKa = 9.89 ATAWAQIEE89 pKa = 4.54 RR90 pKa = 11.84 KK91 pKa = 7.59 TPQLDD96 pKa = 3.59 TSPSLDD102 pKa = 3.57 LDD104 pKa = 3.81 SFLDD108 pKa = 3.73 ARR110 pKa = 11.84 PPCPVSQVSYY120 pKa = 10.52 HH121 pKa = 5.94 SVPLHH126 pKa = 6.57 PEE128 pKa = 3.76 NTCRR132 pKa = 11.84 SGPHH136 pKa = 5.04 VSATRR141 pKa = 11.84 MFGQIYY147 pKa = 9.83 KK148 pKa = 10.4 DD149 pKa = 3.14 KK150 pKa = 11.36 LMTDD154 pKa = 3.44 RR155 pKa = 11.84 LLALEE160 pKa = 4.29 PEE162 pKa = 4.79 FVKK165 pKa = 10.36 EE166 pKa = 3.82 HH167 pKa = 6.93 YY168 pKa = 9.98 YY169 pKa = 10.93 DD170 pKa = 3.42 QVYY173 pKa = 11.15 SDD175 pKa = 3.75 GTAQGLLNRR184 pKa = 11.84 MRR186 pKa = 11.84 KK187 pKa = 9.02 QMSNKK192 pKa = 7.54 VTPLSQLEE200 pKa = 4.38 KK201 pKa = 9.98 VAKK204 pKa = 9.67 TNLSTKK210 pKa = 10.37 RR211 pKa = 11.84 MSTLIGEE218 pKa = 4.44 MFPRR222 pKa = 11.84 KK223 pKa = 8.6 KK224 pKa = 8.99 TLPDD228 pKa = 2.89 WHH230 pKa = 7.39 RR231 pKa = 11.84 DD232 pKa = 2.95 LDD234 pKa = 3.99 LQLEE238 pKa = 4.44 EE239 pKa = 4.58 VVQSAKK245 pKa = 10.75 ASAGFPYY252 pKa = 9.98 CRR254 pKa = 11.84 PKK256 pKa = 11.3 GEE258 pKa = 4.21 VLDD261 pKa = 3.98 QVVCAGIPLLCDD273 pKa = 4.9 AIRR276 pKa = 11.84 EE277 pKa = 4.28 GKK279 pKa = 9.82 LAEE282 pKa = 4.08 FRR284 pKa = 11.84 AANPEE289 pKa = 3.91 FFLCEE294 pKa = 4.08 VKK296 pKa = 10.69 NKK298 pKa = 9.43 RR299 pKa = 11.84 DD300 pKa = 3.38 RR301 pKa = 11.84 YY302 pKa = 7.66 EE303 pKa = 3.79 TAKK306 pKa = 9.15 ITEE309 pKa = 3.96 KK310 pKa = 9.91 TRR312 pKa = 11.84 PYY314 pKa = 10.68 VAFPAHH320 pKa = 5.85 FQLLFSCLSQPFTKK334 pKa = 10.48 DD335 pKa = 2.7 LLLFHH340 pKa = 6.84 EE341 pKa = 5.23 HH342 pKa = 6.6 PKK344 pKa = 10.61 SVNAYY349 pKa = 8.77 GFTMAHH355 pKa = 6.37 GGSSKK360 pKa = 9.92 IRR362 pKa = 11.84 YY363 pKa = 6.49 RR364 pKa = 11.84 TAEE367 pKa = 3.86 LRR369 pKa = 11.84 EE370 pKa = 4.26 LGSKK374 pKa = 10.29 GGAPICRR381 pKa = 11.84 HH382 pKa = 5.96 YY383 pKa = 11.41 GDD385 pKa = 5.16 DD386 pKa = 3.23 ADD388 pKa = 3.55 IYY390 pKa = 10.24 VRR392 pKa = 11.84 RR393 pKa = 11.84 GGKK396 pKa = 8.88 LLRR399 pKa = 11.84 LCPDD403 pKa = 4.16 FSQMDD408 pKa = 3.69 GSVTHH413 pKa = 5.4 EE414 pKa = 4.24 TVRR417 pKa = 11.84 LTVDD421 pKa = 4.06 WILASYY427 pKa = 9.26 EE428 pKa = 4.29 EE429 pKa = 4.21 QWGPSDD435 pKa = 3.66 FWRR438 pKa = 11.84 TIGDD442 pKa = 3.72 LWVEE446 pKa = 4.32 MATSPLFVVHH456 pKa = 7.08 GKK458 pKa = 9.67 DD459 pKa = 3.04 VWEE462 pKa = 4.81 KK463 pKa = 9.12 RR464 pKa = 11.84 TQDD467 pKa = 3.32 GLMTGVVGTTLFDD480 pKa = 3.66 TVHH483 pKa = 6.69 AALAYY488 pKa = 7.21 WVWVEE493 pKa = 3.78 RR494 pKa = 11.84 VHH496 pKa = 6.29 YY497 pKa = 9.28 QRR499 pKa = 11.84 QYY501 pKa = 11.88 ALLEE505 pKa = 3.88 EE506 pKa = 4.6 APAIKK511 pKa = 10.18 LFKK514 pKa = 10.98 EE515 pKa = 3.7 MGLVVKK521 pKa = 10.3 PGTWGFEE528 pKa = 4.26 TVNEE532 pKa = 4.13 NPVQGTLWTTQRR544 pKa = 11.84 FLGVQYY550 pKa = 10.59 KK551 pKa = 9.02 WEE553 pKa = 4.17 TGPRR557 pKa = 11.84 EE558 pKa = 4.42 VEE560 pKa = 4.33 MVPHH564 pKa = 6.92 LAYY567 pKa = 10.18 EE568 pKa = 4.32 DD569 pKa = 3.78 WLSLLLCPRR578 pKa = 11.84 GDD580 pKa = 3.34 EE581 pKa = 4.41 DD582 pKa = 3.61 MRR584 pKa = 11.84 SSRR587 pKa = 11.84 TSEE590 pKa = 3.76 LTRR593 pKa = 11.84 EE594 pKa = 3.95 RR595 pKa = 11.84 VRR597 pKa = 11.84 FDD599 pKa = 3.34 RR600 pKa = 11.84 LRR602 pKa = 11.84 GMIVTGAFSTPEE614 pKa = 3.72 ALAIMYY620 pKa = 8.39 EE621 pKa = 4.55 LIDD624 pKa = 3.9 EE625 pKa = 4.57 VNPVAIIMDD634 pKa = 3.86 VQSGDD639 pKa = 3.74 GKK641 pKa = 11.19 GVTPDD646 pKa = 4.74 AIALLNEE653 pKa = 4.27 DD654 pKa = 4.2 FQWPGSEE661 pKa = 5.62 GVPDD665 pKa = 4.31 WKK667 pKa = 10.46 WCMNLYY673 pKa = 10.44 LSPEE677 pKa = 3.98 NQYY680 pKa = 11.39 EE681 pKa = 3.97 DD682 pKa = 4.4 AQWKK686 pKa = 9.45 LVIPEE691 pKa = 3.95 AHH693 pKa = 6.74 AALSDD698 pKa = 3.43 FKK700 pKa = 11.41 VRR702 pKa = 11.84 YY703 pKa = 9.79 RR704 pKa = 11.84 KK705 pKa = 9.56 DD706 pKa = 3.35 LKK708 pKa = 11.04 KK709 pKa = 10.57 LATVQKK715 pKa = 10.11 DD716 pKa = 3.81 VEE718 pKa = 4.56 GKK720 pKa = 9.48 PSAAAMLPIPDD731 pKa = 5.16 PAPKK735 pKa = 10.26 AQIPSMEE742 pKa = 4.27 IPTSGSRR749 pKa = 11.84 VPKK752 pKa = 10.17 TSEE755 pKa = 4.11 AYY757 pKa = 10.49 NKK759 pKa = 6.78 TTKK762 pKa = 10.39 PVDD765 pKa = 3.41 VTLDD769 pKa = 3.63 DD770 pKa = 3.25 QGLAVEE776 pKa = 4.42 EE777 pKa = 4.29 RR778 pKa = 11.84 TRR780 pKa = 11.84 RR781 pKa = 11.84 PTDD784 pKa = 3.12 VEE786 pKa = 4.32 QILSTFRR793 pKa = 11.84 DD794 pKa = 3.72 LPLSPKK800 pKa = 9.78 AWEE803 pKa = 4.55 EE804 pKa = 3.83 IVAGATEE811 pKa = 4.12 TQRR814 pKa = 11.84 AGLEE818 pKa = 3.96 ALQGGTKK825 pKa = 9.7 VVQPVFQASTLSWKK839 pKa = 10.71 LNWTVSKK846 pKa = 10.89 VLSTAQKK853 pKa = 10.03 AGLVVLPGGPKK864 pKa = 9.8 RR865 pKa = 11.84 KK866 pKa = 9.87 SLITALPPSDD876 pKa = 4.1 PSALEE881 pKa = 4.91 PIDD884 pKa = 3.73 HH885 pKa = 6.92 AAAAVEE891 pKa = 3.99 RR892 pKa = 11.84 ALDD895 pKa = 3.63 NSRR898 pKa = 11.84 PEE900 pKa = 3.82 PKK902 pKa = 9.32 EE903 pKa = 3.32 WSRR906 pKa = 11.84 EE907 pKa = 3.86 HH908 pKa = 6.21 SQLGLSRR915 pKa = 11.84 LKK917 pKa = 11.13 GPLTQEE923 pKa = 3.84 QAAKK927 pKa = 9.81 VCKK930 pKa = 9.94 EE931 pKa = 3.84 FLAHH935 pKa = 7.78 GYY937 pKa = 8.73 RR938 pKa = 11.84 CPMPKK943 pKa = 10.1 CKK945 pKa = 10.02 FGKK948 pKa = 10.51 GNAAPLTFVEE958 pKa = 5.32 VTLHH962 pKa = 6.98 WITNDD967 pKa = 3.14 PHH969 pKa = 7.38 QDD971 pKa = 2.81 KK972 pKa = 11.0 CLLSKK977 pKa = 11.28 CEE979 pKa = 4.22 LHH981 pKa = 7.21 DD982 pKa = 4.28 GCPTGPPMQLRR993 pKa = 11.84 PAKK996 pKa = 10.24 EE997 pKa = 3.29 ITLLKK1002 pKa = 10.16 KK1003 pKa = 9.9 QAEE1006 pKa = 4.31 RR1007 pKa = 11.84 LDD1009 pKa = 4.11 GPQAEE1014 pKa = 4.69 VLPPYY1019 pKa = 9.41 PRR1021 pKa = 11.84 PPLKK1025 pKa = 10.3 FSSMDD1030 pKa = 3.35 LEE1032 pKa = 4.77 EE1033 pKa = 4.28 IRR1035 pKa = 11.84 SIQAEE1040 pKa = 4.58 DD1041 pKa = 3.53 PSMARR1046 pKa = 11.84 SVFSSMCHH1054 pKa = 4.34 EE1055 pKa = 4.18 QGVAVVFTTKK1065 pKa = 9.83 GTDD1068 pKa = 3.31 HH1069 pKa = 7.07 EE1070 pKa = 4.76 KK1071 pKa = 10.98 GAVNAPTYY1079 pKa = 11.39 GEE1081 pKa = 4.16 MHH1083 pKa = 6.14 WTRR1086 pKa = 11.84 IKK1088 pKa = 10.82 KK1089 pKa = 9.65 QGQPTPSFKK1098 pKa = 10.58 RR1099 pKa = 11.84 EE1100 pKa = 3.59 PLAWNLYY1107 pKa = 9.47 LQVLGKK1113 pKa = 10.17 KK1114 pKa = 9.7 RR1115 pKa = 11.84 EE1116 pKa = 4.04 LHH1118 pKa = 5.96 NVLLTTALASLSDD1131 pKa = 3.89 LGKK1134 pKa = 9.88 PSLPPASWYY1143 pKa = 9.24 EE1144 pKa = 3.8 AAKK1147 pKa = 10.96 VEE1149 pKa = 4.4 LLPTASKK1156 pKa = 10.63 AYY1158 pKa = 9.26 STVKK1162 pKa = 10.08 TPAPVLLNSKK1172 pKa = 9.65 PSKK1175 pKa = 9.79 TPLSQSISHH1184 pKa = 7.0 AYY1186 pKa = 6.34 EE1187 pKa = 4.05 TKK1189 pKa = 10.08 PQPSKK1194 pKa = 10.86 PKK1196 pKa = 9.68 TEE1198 pKa = 4.39 SKK1200 pKa = 10.51 PISKK1204 pKa = 9.06 WGKK1207 pKa = 9.37 KK1208 pKa = 9.48 KK1209 pKa = 9.88 PAAPGQAKK1217 pKa = 9.75 KK1218 pKa = 10.72 KK1219 pKa = 9.97 KK1220 pKa = 9.02 GPKK1223 pKa = 8.58 KK1224 pKa = 9.65 PSYY1227 pKa = 9.91 GRR1229 pKa = 11.84 SGATGSLPNRR1239 pKa = 11.84 NSQQ1242 pKa = 3.15
Molecular weight: 138.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.878
IPC2_protein 8.068
IPC_protein 7.878
Toseland 8.083
ProMoST 8.507
Dawson 8.814
Bjellqvist 8.887
Wikipedia 8.829
Rodwell 8.931
Grimsley 8.185
Solomon 8.858
Lehninger 8.843
Nozaki 9.077
DTASelect 8.712
Thurlkill 8.829
EMBOSS 8.99
Sillero 9.063
Patrickios 4.736
IPC_peptide 8.843
IPC2_peptide 7.571
IPC2.peptide.svr19 7.745
Protein with the highest isoelectric point:
>tr|A0A1L3KI24|A0A1L3KI24_9VIRU Uncharacterized protein OS=Sanxia water strider virus 19 OX=1923403 PE=4 SV=1
MM1 pKa = 6.52 PTKK4 pKa = 10.55 RR5 pKa = 11.84 SRR7 pKa = 11.84 NPRR10 pKa = 11.84 NPKK13 pKa = 9.61 PNPNQSQNGAKK24 pKa = 10.23 RR25 pKa = 11.84 NPQPRR30 pKa = 11.84 ARR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GPRR40 pKa = 11.84 SPPMAGVAQQAAFQTGTRR58 pKa = 11.84 NDD60 pKa = 4.16 HH61 pKa = 6.2 IRR63 pKa = 11.84 VTGTDD68 pKa = 3.08 KK69 pKa = 11.32 LYY71 pKa = 11.43 GEE73 pKa = 5.34 DD74 pKa = 4.12 NVPQKK79 pKa = 10.92 HH80 pKa = 5.83 RR81 pKa = 11.84 GEE83 pKa = 4.03 VLLAIRR89 pKa = 11.84 INPASFPRR97 pKa = 11.84 LSRR100 pKa = 11.84 MASTFQKK107 pKa = 10.68 YY108 pKa = 8.61 RR109 pKa = 11.84 FLRR112 pKa = 11.84 MDD114 pKa = 3.54 FRR116 pKa = 11.84 IVSMASTSTSGGYY129 pKa = 9.96 IIGFIPDD136 pKa = 4.31 PDD138 pKa = 4.33 DD139 pKa = 5.94 LADD142 pKa = 3.24 TWTTEE147 pKa = 3.92 QLLSTPHH154 pKa = 5.68 CRR156 pKa = 11.84 IVKK159 pKa = 8.96 AWEE162 pKa = 3.92 SATIGATLEE171 pKa = 4.44 SKK173 pKa = 9.38 WLYY176 pKa = 9.9 TNTGMEE182 pKa = 4.16 SRR184 pKa = 11.84 LYY186 pKa = 10.66 SPGIFVIMVEE196 pKa = 4.31 GSIPGTAPLSVYY208 pKa = 10.16 VDD210 pKa = 3.82 WVAEE214 pKa = 3.87 MHH216 pKa = 6.15 TPSLEE221 pKa = 4.2 LVSKK225 pKa = 9.77 PVQQIEE231 pKa = 4.59 VVKK234 pKa = 10.68 NAYY237 pKa = 10.1 LRR239 pKa = 11.84 DD240 pKa = 3.42 AHH242 pKa = 6.36 FGLWFSDD249 pKa = 3.59 AEE251 pKa = 4.55 GGDD254 pKa = 4.08 DD255 pKa = 3.7 PHH257 pKa = 7.34 TIVPGIVYY265 pKa = 10.1 DD266 pKa = 3.8 VVYY269 pKa = 10.57 KK270 pKa = 10.43 LQSRR274 pKa = 11.84 VYY276 pKa = 11.01 VDD278 pKa = 3.5 YY279 pKa = 10.76 TDD281 pKa = 3.69 QAGSYY286 pKa = 9.78 DD287 pKa = 4.33 KK288 pKa = 10.9 IVATTGDD295 pKa = 3.5 SHH297 pKa = 7.41 GPTLWVADD305 pKa = 4.21 FKK307 pKa = 11.33 GRR309 pKa = 11.84 RR310 pKa = 11.84 IEE312 pKa = 4.26 KK313 pKa = 10.16 EE314 pKa = 3.67 PVKK317 pKa = 10.45 NTFVLEE323 pKa = 4.35 KK324 pKa = 10.63 GDD326 pKa = 3.49 VLTPVQGEE334 pKa = 4.2 VRR336 pKa = 11.84 RR337 pKa = 11.84 GTSCFPRR344 pKa = 11.84 SPLPSSQDD352 pKa = 2.87 GWEE355 pKa = 4.28 LLQKK359 pKa = 10.24 DD360 pKa = 4.43 LRR362 pKa = 11.84 NLSIEE367 pKa = 4.41 GSSKK371 pKa = 11.01
Molecular weight: 41.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.883
IPC2_protein 8.916
IPC_protein 8.902
Toseland 9.502
ProMoST 9.37
Dawson 9.794
Bjellqvist 9.516
Wikipedia 9.984
Rodwell 9.999
Grimsley 9.882
Solomon 9.823
Lehninger 9.78
Nozaki 9.545
DTASelect 9.487
Thurlkill 9.619
EMBOSS 9.94
Sillero 9.721
Patrickios 5.156
IPC_peptide 9.823
IPC2_peptide 8.112
IPC2.peptide.svr19 7.889
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1733
120
1242
577.7
64.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.501 ± 0.694
1.212 ± 0.3
5.309 ± 0.647
6.29 ± 0.703
3.001 ± 0.135
6.52 ± 1.231
2.308 ± 0.16
3.347 ± 0.586
6.694 ± 1.063
9.521 ± 1.517
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.366 ± 0.209
2.25 ± 0.646
8.194 ± 0.635
4.328 ± 0.165
6.636 ± 1.37
7.271 ± 0.317
6.174 ± 1.284
6.463 ± 0.788
1.962 ± 0.289
2.654 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here